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All Journal HAYATI Journal of Biosciences Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter Microbiology Indonesia Jurnal Hortikultura Jurnal Matematika & Sains AGRIVITA, Journal of Agricultural Science Journal of Tropical Biodiversity and Biotechnology Acta Pharmaceutica Indonesia BERITA BIOLOGI Jurnal Biodjati Jurnal Mikologi Indonesia Lumbung: Jurnal Ilmiah Politeknik Pertanian Negeri Payakumbuh Makara Journal of Science Archipelago: Jurnal Pengabdian Kepada Masyarakat Iain Ternate Jurnal Kesehatan Bakti Tunas Husada

I Nyoman Pugeg Aryantha

Kelompok Keahlian Mikrobiologi-Genetika-Biologi Molekuler, Sekolah Ilmu dan Teknologi Hayati, Institut Teknologi Bandung

Author-ID : 284384

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Nursing Public Health Other

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AKTIVITAS LARVISIDAL EKSTRAK ETIL ASETAT DAN HEKSANA DARI FILTRAT Beauveria bassiana TERHADAP Aedes aegypti I Nyoman Pugeg Aryantha; Wahyu Setyaji Dwiantara
BERITA BIOLOGI Vol 18, No 3 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i3.3618

Beauveria bassiana produces several metabolites that are toxic to insects so that it can be used as a biological insect control agent as an alternative to synthetic pesticides. The aim of this study was to determine the larvicidal activity of ethyl acetate and hexane extract from B. bassiana filtrate culture against Aedes aegypti 2nd instar larvae. This research was it cooked by determining the optimum age of spore inoculum of B. bassiana on the Potato Dextrose Agar (PDA) based on the number of spores and its viability. Afterwards, we determine the incubation time of B. bassiana in the Potato Dextrose Broth (PDB) in order to obtain filtrate culture which have highest mortality effects against Ae. aegypti 2nd instar larvae. B. bassiana filtrate culture was extracted with hexane and ethyl acetate and tested aegypti for larvicidal activity with a concentration range of 50, 100, 200, 300 ppm. The LC50 value was carried out by probit analysis. The results showed that ninth day old culture in the PDA was the optimum age of spore inoculum with the spore number and viability were 2.54 x 107 spore/mL and 93.46% respectively. The filtrate of sixth day old culture in PDB medium gave 100% mortality against 2nd instar Ae. Aegypti larvae. LC50 values of ethyl acetate and hexane extract were 117.28 dan 287.09 ppm. These results showed that the ethyl acetate and hexane extract of B. bassiana filtrate culture have biopesticide potential against 2nd instar Ae. aegypti larvae.

Development of Simple-Sequence Repeats Markers from Durian (Durio zibethinus Murr. cultv. Matahari) Genomic Library Panca Jarot Santoso; Adi Pancoro; Sony Suhandono; I Nyoman Pugeg Aryantha
AGRIVITA, Journal of Agricultural Science Vol 39, No 3 (2017): OCTOBER
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v39i3.1171

Simple sequence repeats have been proved as powerful markers and widely used in molecular breeding to reduce cycles and cost efective. The availability of the marker is, however, very limited in durian. This research aimed to develop SSR markers from durian genomic library. Genomic DNA was isolated from durian shoot leaf, whilst SSR motifs were isolated using membrane-based oligonucleotide enrichment hybridization protocol. Annotation made on the library found 527 unique motifs from 354 durian libraries which form 425 loci. The SSR motifs obtained were generally short repeats which reached 89.6 %, whilst longer repeats were found consisted of compound motifs. Eleven loci were selected as representative for further test to prove their informativity. A number of unique allels were successfully amplified from 17 durian genomes. The analysis showed the polymorphic information content (PIC) values ranged from 0.000 to 0.662 with an average of 0.390. The SSR loci also showed their ability to be used for durian diversity analysis as the evident that the loci could be used as genetic markers for assisting further durian breeding program.

Upaya Kultivasi Jamur Morel (Morchella esculenta (L.) Pers.) dari Kawasan Lembang, Jawa Barat dan Optimasi Pertumbuhannya dengan Menggunakan Media Pati Adhityo Wicaksono; Reza Raihandhany; I Nyoman Pugeg Aryantha
Jurnal Mikologi Indonesia Vol 5, No 1 (2021): Juni 2021
Publisher : Perhimpunan Mikologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46638/jmi.v5i1.165

Jamur morel (Morchella esculenta (L.) Pers.) memiliki nilai gizi yang baik dan bernilai jual tinggi dari segi industri kuliner. Di Indonesia, jamur ini telah ditemukan di kawasan Gunung Rinjani, Nusa Tenggara Barat, dan di kawasan Lembang, Jawa Barat, yang menjadi spesimen dalam penelitian ini. Penelitian ini bertujuan untuk mencoba menginokulasi jamur morel pada media kultur murni, media bibit induk, dan media tanam. Kemudian pertumbuhan miselia dan laju tumbuhnya diamati pada media karbohidrat yang dibuat dari tepung terigu, beras, maizena, dan tapioka pada cawan Petri. Kultur murni dengan menggunakan media agar dekstrosa kentang di cawan Petri telah membuahkan hasil, sebagaimana jamur morel telah membentuk miselia dan sklerotia pada media kultur. Hasil positif juga ditemukan pada tahap lanjutan, yaitu kultur bibit induk dengan media buah kapuk dan dedak. Akan tetapi, upaya kultur di baglog berisi media tanah kompos belum membuahkan hasil, sehingga perlu diteliti lebih lanjut dan dilakukan optimasi. Pada percobaan optimasi media karbohidrat, miselia jamur morel tumbuh paling baik pada media tepung terigu, kemudian tepung beras, dan paling sedikit pada tepung maizena. Miselia tidak tumbuh pada media tepung tapioka.

ISOLASI DAN IDENTIFIKASI BAKTERI PELARUT FOSFAT DARI TANAH RHIZSOSFER KELAPA SAWIT (Eleis guineensis) Asrul Asrul; I Nyoman Pugeg Aryantha
LUMBUNG Vol. 19 No. 1 (2020): Februari
Publisher : Politeknik Pertanian Negeri Payakumbuh

Bakteri sebagai salah satu agent penyubur tanah, masih sangat minim diketahui petani. Kenyataannya bakteri mampu menyediakan unsur hara makro yang dibutuhkan oleh tanaman, contohnya fosfat. Fosfat di tanah umumnya dalam bentuk terikat, baik yang berbentuk anorganik (Ca-P, Fe-P dan Al-P) maupun organik (asam nukleat dan fosfolipida). Mengeksresikan asam organik, merupakan cara umum bakteri untuk melarutkan fosfat terikat khususnya yang anorganik. Isolasi bakteri pelarut fosfat dilakukan di tanah rhizosper Kelapa Sawit (Eleis guineensis) PT Astra Agro Lestari dengan menggunakan media pikovskaya yang di dalamnya terdapat kalsium fosfat (Ca-P). Hasilnya ada 6 bakteri yaitu A2, B5, C7, F1, F2, dan K1 yang mampu melarutkan kalsium fosfat (Ca-P). Uji kuantitatif dengan menggunakan spektrofotometer 410 nm, pada supernatant pikovskaya cair menunjukkan bakteri A2 mampu melarutkan fosfat 20,40 ppm paling baik jika dibandingkan dengan bakteri, C7= 7,69 ppm, F1= 5,77 ppm, F2= 5,53 ppm, B5= 1.19 ppm dan K1= 0,46 ppm. Identifikasi partial sequences 16s rRNA menunjukkan bakteri A2 putative Amycolatopsis albidoflavus.

Diversity of Culturable Bacterial in Various Parts of Luwakâ€™s (Paradoxurus hermaprodithus javanica) Gastrointestinal Tract SONY SUHANDONO; HERI SETIADI; TATI KRISTIANTI; ALI BUDHI KUSUMA; ANDINI WARIH WEDARINGTYAS; DEMI TRISTAN DJAJADI; I NYOMAN PUGEG ARYANTHA
Microbiology Indonesia Vol. 10 No. 2 (2016): June 2016
Publisher : Indonesian Society for microbiology

Luwak coffee is a highly-priced coffee produced exclusively by the palm civet or luwak (Paradoxurus hermaphrodites ssp.). The purpose of this study was to determine the diversity of culturable bacteria in the gastro intestinal tract of luwak. The bacterial isolates were phenotypically characterized by their morphology and molecularly by analysis of their1,500bp 16s rDNA sequence. The results showed that Enterobacter cloacae and Lactobacillus brevis were found all over luwakâ€™s digestive tract. Enterobacter cloacae was the most common species. The most diverse bacterial population was found in small intestine. Seven bacterial generawere successfully identified from the small intestine and colon, compared to only five genera found in the stomach.

Potential MGPB In Optimizing Paddy Straw Mushroom (Volvariella volvacea WW-08) Growth WINDI SILVANI JEMSI; I NYOMAN PUGEG ARYANTHA
Microbiology Indonesia Vol. 11 No. 2 (2017): Juni 2017
Publisher : Indonesian Society for microbiology

Paddy straw mushroom (Volvariella volvacea) contains high protein content and delicious flavor, makes it highly demand each year. Production of V.volvacea does not merit the requirements due to its limited production. Therefore, approach in increasing production using mushroom growth promoting bacteria (MGPB) are needed. This study aims to obtain MGPB isolate as potential agent to increase V. volvacea strain WW-08 growth. This is experimental research in laboratory that consisted of indigenous bacteria isolation, MGPB screening with dual culture, MGPB inoculum optimization, molecular identification of selected MGPB using 16S rRNA, protein profiling with SDS-PAGE, and fruting body production. Indigenous bacteria obtained from growth medium were 58 isolate, and W34 bacteria at concentration of 106 sel/ml showed most significant result on micellium growth. Sequence of 16S rRNA region showed W34 bacteria is Bacillus cereus. Visualization of SDS-PAGE showed new protein in result of interaction between Bacillus cereus and V. volvacea strain WW-08 with molecule weight of 17kDa. Average of fruting body of V. volvacea strain WW-08 in treatment of B. cereus harvested for 7 days, was 240.19g, whereas without treatment of B. cereus was 82.15g. These findings indicate treatement of B. cereus strain W34 increase V. volvacea WW-08 growth by 300%.

CONCEPTION OF ISLAMIC RELIGIOUS EDUCATION (PAI) ON EVOLUTION CONCEPT Susanti Wulandari; Nuryani Y. Rustaman; Ari Widodo; I Nyoman Pugeg Aryantha
Archipelago Vol 1, No 2 (2020): Edisi Desember 2020
Publisher : IAIN Ternate

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4633/arc.v1i2.809

Evolution is one of the content that is considered controversial in science because many people do not agree with the theories of evolution. This study aims to investigate the perceptions of Islamic religious education students’ regarding the concepts of evolution. This descriptive research was conducted through a survey method to 288 first-level students’ at a private university in Tasikmalaya Regency, West Java. The findings show that most of the PAI students’ have a wrong understanding of the concept of evolution. Based on the results of the interview, the fact is that this is due to the knowledge obtained at the previous level. This research indicates that there are still many misconceptions that occur in students’, including science or biology teachers who teach evolutionary material at the secondary school level.Keywords: misconception, evolution concept, Islamic religious education

Effect of ethanol extract of Ganoderma lucidum from Tasikmalaya against the growth of Salmonella sp bacteria and some Pathogenic Fungi using the Kirby-Bauer method in vitro Khusnul Khusnul; Meti Kusmiati; Rianti Nurpalah; I Nyoman Pugeg Aryantha; Tutus Gusdinar Kartawinata
Jurnal Kesehatan Bakti Tunas Husada: Jurnal Ilmu-ilmu Keperawatan, Analis Kesehatan dan Farmasi Vol 22, No 2 (2022)
Publisher : LPPM Universitas Bakti Tunas Husada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36465/jkbth.v22i2.956

Ganoderma lucidum (lingzhi) can be used as traditional medicine. This mushroom has many properties, one of which is as an antimicrobial. Antimicrobial functions as antibacterial, antiviral, and antifungal. Lingzhi contains organic alkaloid compounds, flavonoids, and triterpenoids. One of the bacteria that often cause disease in Indonesia is Salmonella sp and several dermatophyte diseases caused by infection with several pathogenic fungi such as Malassezia furfur, Trichophyton rubrum, and Trichophyton mentagrophytes . This study aimed to determine the inhibitory power of Lingzhi from Tasikmalaya ethanol extract against Pathogenic Microorganism. The method used is tested against the anti-microbial used by using the diffusion method using disc paper or Kirby Baure. The analysis showed that the 70% ethanol extract of Lingzhi mushrooms had antibacterial activity against salmonella sp as evidenced by the presence of an average inhibition zone at a concentration of 50% (16,57mm), 60% (18,1mm); 70% (18,55mm), 80% (19.17mm), 90% (21,2mm), and 100% (21,8mm). Inhibitory ability against several pathogenic fungi was able to inhibit at a concentration of 100% with a diameter of Trichophyton rubrum, Trichophyton mentagrophytes and Malassezia furfur with an average diameter of inhibition of 12.9 mm, 9.4 mm, and 6 mm.

Enhancing Lovastatin Biosynthesis in Oyster Mushrooms (Pleurotus ostreatus) using Phytohormones Mustafa, Fransisca Astrid; Kandar, Mamat; Aryantha, I Nyoman Pugeg
Makara Journal of Science Vol. 27, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

The biosynthesis of lovastatin, the anti-cholesterol compound, in oyster mushrooms (Pleurotus ostreatus), has the potential to be enhanced by utilizing phytohormones, which activate the expression of certain genes. This research aimed to determine the best type and concentration of phytohormone among auxin, gibberellin, and cytokinin, as well as the best mycelial colonization percentage in F2 medium to realize the greatest increase in lovastatin biosynthesis in oyster mushrooms. Lovastatin was extracted from the fruiting bodies and mycelia and analyzed by spectrophotometry. The analysis of CYP450 linked to lovastatin biosynthesis was conducted by quantitative polymerase chain reaction (qPCR) using samples containing the highest concentration of lovastatin. The results showed that adding phytohormones increased the lovastatin concentration in the fruiting bodies and mycelia of P. ostreatus. The highest lovastatin content was observed in the 10 ppm gibberellin treatment by applying 75% mycelial colonization in the F2 medium. Furthermore, this gibberellin treatment also demonstrated increased expression of the CYP450 gene in the fruiting bodies and mycelia. In conclusion, phytohormone treatments with the right timing and appropriate concentration increased lovastatin biosynthesis in P. ostreatus as well as related gene expression.

Co-Authors Adhityo Wicaksono Adi Pancoro Adi Pancoro ANDINI WARIH WEDARINGTYAS Andriana Susilowati Ari Widodo Asrul Asrul Darah Ibrahim DEMI TRISTAN DJAJADI Dwiantara, Wahyu Setyaji Enggar Utari Ewi Awaliyati FENNY MARTHA DWIVANY HERI SETIADI Indah Juwita Sari Indria Wahyuni Irmanida Batubara Karlina, Yenni Khusnul, Khusnul KUSUMA, ALI BUDHI Latifah Kosim Darusman LISTYA UTAMI KARMAWAN Mamat Kandar Mashuri Waite Meti Kusmiati Mustafa, Fransisca Astrid Nampiah Sukarno Nuryani Rustaman Panca Jarot Santoso Panca Jarot Santoso Rahmat Ariffudin Reza Raihandhany Rianti Nurpalah Rida Oktorida Khastini Rizkita Rachmi Esyanti Rohani Cinta Badia Ginting Sihegiko Kanaya SONY SUHANDONO Sony Suhandono Sukrasno Sukrasno Sukrasno, Sukrasno Susanti Wulandari Syarifah A. Rashid TATI KRISTIANTI Tutus Gusdinar Kartawinata Utut Widyastuti Wahyu Setyaji Dwiantara WINDI SILVANI JEMSI Yenni Karlina Yuniar Mulyani

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