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Study Motility of Ongole Crossbred Semen with Soybean-Based Diluents Stored at Ambient Temperature (24-27 0C) Dian Ratnawati; Gatot Ciptadi; sri rahayu; trinil susilawati
Jurnal Ilmu-Ilmu Peternakan Vol. 33 No. 1 (2023): April 2023
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2023.033.01.011

Abstract

Artificial insemination with fresh semen is an alternative that can be done if there is a limitation of liquid nitrogen, bull, and facilities for processing liquid and frozen semen (costly). This study aimed to determine the optimal diluent formula based on CEP-3 (modified/m) and aqueous soybean extract (ASE) to support spermatozoa motility at ambient temperature storage. The research design was completely randomized, with eight treatments and six replications. The diluent formula treatments were as follows: T1 (CEP-3(m)), T2 (CEP-3(m)+10% Egg Yolk/EY), T3 (CEP-3(m)+ASE 1%), T4 (CEP-3 (m)+ASE 2%), T5 (CEP-3(m)+ASE 3%), T6 (CEP-3(m)+ASE 4%), T7 (CEP-3(m)+ASE 5%), and T8 (CEP-3(m)+ASE 6%). The parameters were measured, i.e., the motility of spermatozoa and its kinetic parameters. Data were analyzed using ANOVA with SPSS 16 program. The result showed that the progressive motility of spermatozoa at day one was more than 50% in diluents CEP-3(m)+ASE 1-2%. Diluent CEP-3(m) and aqueous soybean extract 1-2% can support spermatozoa's motility and kinetic parameters during storage at ambient temperature, including progressive motility, motility, motility, velocity, and BCF.
Comparative Study of Feeding and Nutrition for Beef Cattle in Smallholder Farmers: Case Study in Regencies of Malang, Pamekasan, and Tuban: Comparative Study of Feeding and Nutrition for Beef Cattle in Smallholder Farmers: Case Study in Regencies of Malang, Pamekasan, and Tuban Asri Nurul Huda; Kusmartono; Poespitasari Hazanah Ndaru; Trinil Susilawati; Mashudi
Jurnal Ilmu Nutrisi dan Teknologi Pakan Vol. 21 No. 1 (2023): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jintp.21.1.35-40

Abstract

This study evaluated feeds for fattening and breeding beef cattle in smallholder farmers located in Malang, Pamekasan, and Tuban regencies. The research was conducted using two methods i.e., survey and laboratory analysis. The data survey was carried out in each area, and 30 respondents (n=30) were observed per area in classification of low to medium livestock ownership. Laboratory analysis was performed to analyse the chemical composition of the feed and to measure the total gas production. The data analysis approach used quantitative descriptive to describe the nutritional value quality of beef cattle feed with available quantitative data. The results showed variations in the nutrient content of the beef cattle feed in three different study locations in smallholder farmers located in Regency of Malang, Pamekasan, and Tuban while the total gas production showed a high production in the fattening cattle feeds between three study locations. Thus, it was concluded that the feed for fattening cattle had better nutrient content in terms of crude protein content and total gas production than the breeding cattle feed. Key words: cattle feed, nutrition, gas production
Evaluation The Success of Artificial Insemination Using Frozen Sexed Semen Based on Different Estrus Characters Putri Utami; Muhammad Zainul Hanif; Aulia Puspita Anugra Yekti; Rizki Prafitri; Asri Nurul Huda; Kuswati Kuswati; Kusmartono Kusmartono; Trinil Susilawati
Jurnal Agripet Vol 22, No 2 (2022): Volume 22, No. 2, Oktober 2022
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v22i2.23423

Abstract

ABSTRACT. Knowledge of farmers about estrus detection is one of the factors that affect fixed-time artificial insemination (AI). This study aims to evaluate the success rate of AI using Y sexing frozen semen based on the estrus character of cattle. Forty-five Limousin Crossed Cows involved in this research with a Body Condition Score of 3-5 (1-9 scale), 1.8-7 years old. Y sexing frozen semen is produced by the Singosari Center for Artificial Insemination using Percoll's Gradient Density Centrifugation method. Observation of estrus character was done before insemination with the deep insemination technique of AI. Artificial insemination was carried out using double doses at the 2nd and 8th hours after estrus. Rheinbio vitamins as BioATP+ are injected after AI. The results showed that the Conception Rate and Pregnancy Rate based on the estrus character was higher as indicated by the red colour of the vulva was 17.78% and 22.22%; very swollen vulva was 20% and 22.22%; abundant cervical mucus was 15.56% and 17.78%, and vaginal temperatures ranging from 38.0-38.5oC was 13.33% and 20%. In conclusion, the low percentage of pregnancy in this study was caused by various factors, mainly the genetic quality, early embryonic efficacy, maintenance management especially feed, and there were cows that experienced reproductive disorders.(Evaluasi keberhasilan inseminasi buatan menggunakan semen beku sexing berdasarkan karakter estrus yang berbeda)ABSTRAK. Pengetahuan peternak mengenai deteksi estrus merupakan salah satu faktor yang berpengaruh terhadap ketepatan waktu inseminasi. Penelitian ini bertujuan untuk mengevaluasi tingkat keberhasilan IB menggunakan semen sexing Y berdasarkan karakter estrus ternak. Akseptor yang digunakan pada penelitian ini berjumlah 45 ekor Sapi Persilangan Limousin dengan BCS 3-5 dan umur berkisar antara 1,8-7 tahun. Semen beku sexing Y merupakan hasil produksi Balai Besar Inseminasi Buatan (BBIB) Singosari menggunakan metode Sentrifugasi Densitas Gradien Percoll (SGDP). Pengamatan karakter estrus dilakukan sebelum inseminasi. Inseminasi Buatan dilakukan dengan menggunakan double dosis pada jam ke-2 dan jam ke-8 dengan teknik deep insemination. Vitamin Rheinbio sebagai BioATP+ diinjeksikan setelah IB. Hasil penelitian menunjukkan bahwa keberhasilan kebuntingan ditinjau dari nilai conception rate (CR) dan pregnancy rate (PR) berdasarkan karakter estrus ternak lebih tinggi ditunjukkan pada kondisi warna vulva merah merata yaitu 17,78% dan 22,22%; vulva yang sangat bengkak yaitu 20% dan 22,22%; banyak mengeluarkan lendir servik yaitu 15,56% dan 17,78%, serta suhu vagina yang berkisar antara 38,0-38,5oC yaitu 13,33% dan 20%. Persentase kebuntingan yang rendah pada penelitian ini disebabkan oleh berbagai faktor, utamanya kualitas genetik ternak, kematian embrio dini, manajemen pemeliharaan khususnya pakan, dan terdapat ternak yang mengalami gangguan reproduksi.