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Journal : HAYATI Journal of Biosciences

Effectiveness of Biolarvicides of Imperata cylindrica, Saccharum spotaneum and Andropogon aciculatus on Aedes aegypti larval Mortality and Egg-laying Ability in Adults Listiono, Heru; Damiri, Nurhayati; Kamaluddin, Totong; Irsan, Chandra; Rahim, Supli Effendi
HAYATI Journal of Biosciences Vol. 32 No. 3 (2025): May 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.3.649-660

Abstract

Vector-borne disease such as Dengue Hemorrghagic (DHF), transmitted by Aedes aegypti and Aedes albopictus, remain a significant public health concern in Indonesia. Controlling these disease often involves insectides; however, the negative impact of chemical insecticides have prompted interest in organic alternatives derived from plants. Certain weeds, including cogon grass (Imperata cylindrica), wild sugarcane (Saccharum spontaneoum), and needle grass (Andropogon aciculatus), have shown potential as botanical insecticides. Research findings showed that weed root extracts significantly affect larval mortality rate of Ae. Aegypti. At 1000 ppm, larval mortality was significantly higher compared to 100 ppm and the control, while treatments of 1 ppm and 10 ppm showed similar results to the control. Probit analysis revealed that I. cylindrica root extract achieved an LC50 of 974.99 ppm within 24 hours, indicating it could kill 50% of Ae. Aegypti larvae. Within 48 hours, the LC50 dropped to 889.20 ppm. Toxicity tests further revealed significant differences in Ae. Aegypti egg-laying abilities when treated with extracts. Analysis of variance yielded p-values of 0.000 for egg hatching within 72 and 96 hours, highlighting significant differences across samples. These findings suggest the extracts influence mosquito reproduction, warranting further studies to assess the quality of egg hatched from larvae exposed to these treatments. The potential of botanical insecticides derived from weeds represents a promising step toward sustainable mosquito control in the fight against vector-borne diseases.
Endophytic Entomopathogenic Fungi Negatively Impact on Growth and Development of Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae) Herlinda, Siti; Milinia Puspita Sari, Jelly; Elfita; Suwandi, Suwandi; Anggraini, Erise; Hong Lau, Wei; Arrwais, Rhani; Irsan, Chandra; Hamidson, Harman; Hasyim, Hamzah; Verawaty, Marieska
HAYATI Journal of Biosciences Vol. 32 No. 6 (2025): November 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.6.1564-1576

Abstract

An alternative method for controlling Spodoptera frugiperda applies entomopathogenic fungi (EPF). The present research aimed to determine the impact of the endophytic EPF  on the growth and development of S. frugiperda. Twenty molecularly identified isolates of the endophytic EPF were used in seed treatments with the fungal suspension 1 × 1010 conidia.mL-1. The endophytic EPF that colonized corn leaves eaten by the neonate S. frugiperda larvae was able to significantly decrease the body weight of the larvae and pupae. The endophytic EPF could prolong the developmental time of all instar larvae and pupae.  The endophytic EPF could raise larval and pupal mortality and reduce the ability of adults to emerge from pupae,  and decrease egg laying by females.  The lowest percentage of adult emergence was observed in the adults from larvae consuming corn leaves colonized with Beauveria bassiana JaGiP and JgSPK isolates, which were 34.67% and 24%, respectively. Consequently, a reduced adult emergence could lead to a high cumulative death rate of 76% from larvae to adults. Finally, the endophytic EPFs negatively affect S. frugiperda growth and development.  B. bassiana JaGiP and JgSPK isolates are the most pathogenic fungi in inhibiting the growth and development of S. frugiperda. They are promising candidates for controlling S. frugiperda in the corn field. 
Co-Authors A. Muslim Abu Umayah Aditya Warman Afifah, Miftah Agustin, Hera Agustin, Selvia Ahmad , Muslim Ahmad Muslim Amalia Rahmadani, Shinta Apriliani Putri, Ochi Apriyani, Mella Arif Hidayat ARINAFRIL ARINAFRIL Arrwais, Rhani Arsi, Arsi Artika, Aziza Arum Setiawan Astuti Kurnianingsih Azim, Saida Fitriani Bambang Gunawan Benyamin Lakitan Dwiyanti, Hana Elfita Ellisa Riyanti, Tia Erise Anggraini, Erise Fani, Gio Hamidah Aulia Hamzah Hasyim Harman Hamidson Harman, Hamidson Hefi Sugiarto, Muhammad HERU LISTIONO Hidayat, Ryan Hilda Zulkifli Hong Lau, Wei ISTIQOMAH, HAWARYAH Kamaluddin, Totong karenina, Tili Khabibah, Nuril Khotimah, Dini Husnul Laila, Mey Rahma Lailaturrahmi Latutoibin, Risal Lisdiana Lisdiana M. Umar Harun Margaretha, Shera Marieska Verawaty Marlinda, Astry Nurhalifa Mawarni, Aprilliyah Milinia Puspita Sari, Jelly Mirsya, Sabryna Aulia Mulawarman Mulawarman Nisa, Nabila Aulia Nuraini, Feni Nurhayati Damiri Oktaviani oktaviani Pratama, Risky Yuda Puspita Sari, Despi Rahmad Fadli Rahmat Pratama, Rahmat Riana, Nanda Risda Muli, Risda Riswan Riswan Rizkie, Lilian Salam, Beni Saputra, Eka Bayu Saputra, Muhamad Ridho Saripudin Saripudin Sefrilla, Marlin Setiawan, Irenius Tegar SHK, Suparman Shobiha, Salma Naswa SITI HERLINDA Sofia Sandi Suheyanto Suheryanto Suparman Suparman SUPLI EFFENDI RAHIM Susilawati . Susilawati, Susilawati Suwandi Suwandi . Suwandi Suwandi Syabella, Tantri Tiran Gunasena, Mahesh Umar Harun, Umar Umayya, Larasati Nur Weri Herlin Wiwik Handayani YULIA PUJIASTUTI