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All Journal Jurnal Sain Veteriner METAMORFOSA Journal of Biological Sciences ASPIRATOR BIOTROPIC The Journal of Tropical Biology Jurnal Biologi Tropis Jurnal Entomologi Indonesia Jurnal Abdi Insani BALABA (JURNAL LITBANG PENGENDALIAN PENYAKIT BERSUMBER BINATANG BANJARNEGARA) BioEksakta : Jurnal Ilmiah Biologi Unsoed ASPIRATOR - Journal of Vector-Borne Diseases Studies
Trisnowati Budi Ambarningrum
Fakultas Biologi, Universitas Jenderal Soedirman
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Journal : BioEksakta : Jurnal Ilmiah Biologi Unsoed

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Efektivitas Zat Aktif Fipronil pada Berbagai Substrat Fagostimulan untuk Pengendalian Kecoak Jerman (Blattella germanica L.) Maula, Riska Isna; Pratiknyo, Hery; Susilo, Untung; Ambarningrum, Trisnowati Budi
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.2.1859

Abstract

German cockroach (Blattella germanica L.), is a residential insect pest and vector of various diseases. To control its population, alternative chemical control models with Fipronil 0.03% are now being developed. Fipronil has been proven to be effective and has no resistance. To increase the effectiveness of the bait in controlling German cockroaches, a fipronil 0.03% combination needs to be developed with a phagostimulant bait of bananas, durian, erythisol sugar, and sugar formulation, so this study needs to be done. This study used an experimental method with a randomized block design (RBD), five treatments such as A: bait with a combination of banana phagostimulant and 0.03% fipronil, B: bait with a combination of durian phagostimulant and 0.03% fipronil, C: bait with a combination erythisol phagostimulant and 0.03% fipronil, D: bait with a combination of sugar phagostimulant and 0.03% fipronil, K: bait with a combination of matrix and fipronil 0.03% (control positive) was used for the interest test, and bait with a matrix without fipronil 0.03% (control negative) was used for mortality testing. The observations the average results for durian, banana, sugar formulated, erytrisol sugar and controls, respectively 20%, 20%, 21.6%, 12.4%, and 22.8%, while the results bait consumed with durian stimulation are 0.29g, banana 0.31g, sugar formulation 0.28g, erytrisol sugar 0.26g and control 0.24g. However, based on statistical tests, the results of the interest test with the feed consumption test between treatments showed no significant difference (p>0.05). The test results in mortality of cockroaches Germany's feed with phagostimulan durian, banana, formulations sugar, sugar erytrisol and control consecutively 98% 100 %, 98%, 98%, and 20%, based on the statistical test there is a significant difference (p<0.05), where the difference based on the Duncan test is their treatment compared to control.
Ketertarikan dan Kesukaan Kecoak Jerman, Blatella germanica L. (Dictyoptera: Blattellidae) Terhadap Fagostimulan Berbeda Ilhami, Femilia Hajar; Ambarningrum, Trisnowati Budi; Pratiknyo, Hery
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.2.1983

Abstract

German cockroaches (Blatella germanica) L. are includes in settlement bugs that are hard to terminate. One alternative to control German cockroaches that are using bait. To make bait formulation, the first thing is look for the type of phagostimulant that can be interesting and attractive to German cockroaches. The purpose of this research is to study the type of phagostimulant that has the ability to attract and liked by German cockroaches. Phagostimulant types used are bananas, durian, sugar formulations, and erythrisol. Data from observations of interest in test interest were analyzed using the t test and calculation of the attraction index, while the data for German roaches favored were analyzed using ANOVA and index feeding calculations. The results obtained were that German cockroaches were attracted to bananas, durian, sugar formulations, and erythrisol. ANOVA analysis results obtained sig 0.029 (p <0.05). The results of the calculation of the attractiveness index and feeding index obtained German cockroaches are preferred by bananas compared to other phagostimulants. The conclusion of this study is the type of phagostimulant that can attract cockroaches Germany is banana, durian, sugar formulation, and erythrisol, while the type of phagostimulant most preferred by German cockroaches is banana.
Perilaku Memilih Umpan Dengan Fagostimulan Yang Berbeda Pada Kecoak Jerman Blattella germanica L. (Dictyoptera: Blattellidae) Aliefia, Rizky Arjunnajat; Ambarningrum, Trisnowati Budi; Basuki, Edi
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 3 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.2007

Abstract

German cockroach (Blattella germanica L.) is one of the important residential pest insects, because of its role as a vector of disease. To reduce its population, it can be used with bait techniques. However, there was a rejection of glucose against German cockroaches against phagostimulant-based commercial bait. Therefore it is necessary to review the phagostimulant as a component of the bait to minimize failure to control German cockroaches based on bait using German cockroaches strain VCRU (Vector Control Research Unit). The first step to formulating the bait is to find the phagostimulant most sought by German cockroaches. The ingredients used as fagostimlan are sugar, durian, erythritol, and banana. The purpose of this study was to determine the behavior of choosing German cockroaches against bait with different phagostimulants and the peak of feeding activity in German cockroaches. This study used an experimental method with a Completely Randomized Design (CRD) consisting of 4 treatments giving phagostimulant and repeated 4 times divided into four test arenas. The treatment consisted of bait which each contained sugar, durian, erythritol, and banana as phagostimulants. Observation of behavior using the method of behavior sampling is recorded in continuous recording for 24 hours using a Closed Circuit Television (CCTV) camera. The parameters measured were latency, frequency, duration, and peak feeding activity of German cockroaches. The data were analyzed with ANOVA at p <0.05. The results showed that the behavior of selecting cockroaches from the VCRU strain on the bait was not significantly different (p<0.05) and was attracted to all feeds provided, but gel bait with sugar phagostimulant was the preferred bait by looking at the three parameters of latency, frequency, and duration. The results of the average latency in the VCRU strain to the durian gel for 17 minutes, the average frequency of most visits to the sugar gel in the VCRU strain by 10 times, the longest average duration of the VCRU strain gel for 1 minute 46 seconds and peak eating activity VCRU strains occur between 17:00 - 20:00.
Kelimpahan Tungau Predator pada Tanaman Melati Gambir (Jasminum officinale) di Desa Cipawon, Bukateja, Purbalingga, Jawa Tengah Rakhmayani, Ika; Budianto, Bambang Heru; Ambarningrum, Trisnowati Budi
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 1 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3927

Abstract

Tanaman melati gambir (Jasminum officinale) yang terdapat di Kecamatan Bukateja, Desa Cipawon, Purbalingga, Jawa Tengah merupakan salah satu komoditas bernilai ekonomi tinggi, kegunaannya tidak hanya sebagai tanaman hias, tetapi sebgai bahan pengharum the, kosmetik, parfum, dan lain-lain. Salah satu penyebab penurunan produktivitas Melati Gambir di disebabkan oleh adanya serangan hama yaitu tungau. Populasi tungau hama dikendalikan oleh tungau predator. Penelitian ini bertujuan untuk mengetahui jenis-jenis tungau predator dan menentukan kelimpahan tungau predator pada tanaman Melati Gambir di Desa Cipawon, Purbalingga, Jawa Tengah. Metode penelitian ini menggunakan metode survey dengan teknik pengambilan sampel secara purposive sampling. Terdapat sembilan titik sampling, delapan titik di tepi luar dan satu titik di tengah perkebunan. Setiap sampling diwakili oleh tiga pohon. Sampel berupa daun melati gambir berasal dari 10 tangkai daun terbawah diperoleh sebanyak 270 sampel daun. Masing-masing titik sampling diambil sebanyak tiga kali ulangan dengan interval waktu satu minggu. Variabel yang diamati meliputi jumlah individu dan jumlah spesies tungau predator pada tanaman melati gambir dan parameter yang dihitung yaitu luas daun, sudut duduk daun, panjang dan kerapatan trikoma, temperature, kelembapan serta curah hujan. Data yang diperoleh dianalisis dengan analisis variansi (ANOVA) dengan tingkat kesalahan 20%. Hasil identifikasi tungau predator pada tanaman melati gambir didapatkan dua spesies yaitu Amblyseius largoensis dan Phytoseius amba. A. largoensis sebanyak 76 individu dengan presentase kelimpahan 61,8% dan P. amba sebanyak 47 individu dengan presentase kelimpahan 38,2%. Kelimpahan tungau predator tergolong sedang karena predator mudah dijumpai, setiap tiga daun dijumpai satu predator.
Frekuensi Pemberian Pakan Limbah Carica (Vasconcellea pubescens A.DC) terhadap Perkembangan Larva Black Soldier Fly (Hermetia illucens L.) Nuranisah, Annanda; Ambarningrum, Trisnowati Budi; Atang, Atang; Haryanto, Trisno; Setiyono, Eko
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 2 (2022): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.2.4621

Abstract

Carica (Vasconcellea pubescens A.DC) waste in Wonosobo can be a problem. Black Soldier Fly/ BSF (Hermetia illucens L.) is biodegradator insect can be right solution. BSF able to convert organic waste into nutrients for growth and development. The purpose of this study was to determine the effectiveness of the frequency of feeding carica industrial waste on the development of BSF larvae, to determine the effect of feeding frequency on the increase in larval biomass and to determine the survival rate of BSF larvae. The study used an experimental method with RAL factorial. The first factor is type of feed, carica and chicken pellets. Frequency of feeding as the second factor is the frequency of once a day, every two days and three days. Variable of the experiment is head capsule, number of prepupae larvae, larval biomass and survival rate. The data obtained were analyzed by ANOVA at an accuracy level of 95%, if it had a significant effect, it would be continued with the DMRT test. The results of the study The frequency of feeding both once a day (F1), twice a day (F2), and every three days (F3) had no effect on the development of BSF larvae (P> 0.05). The frequency of feeding affects biomass of larvae, the frequency of feeding every day (F1) has the highest biomass while the mechanism of feeding frequency every three days (F3), although given the same quantity of feed weight per day has the lowest biomass. The survival rate of carica feeding is lower, which is about 60-70% at the three different frequencies with chicken pellet feeding which still reaches 90% in each treatment.
Komposisi Lalat pada Bangkai Mencit (Mus musculus) Setelah 10 Hari Kematian di Darat dan di Air Nirwani, Shintya Nitra; Ambarningrum, Trisnowati Budi; Budianto, Bambang Heru
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 5 No 2 (2023): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2023.5.2.5101

Abstract

Penelitian ini bertujuan untuk mengetahui perbedaan komposisi lalat yang meliputi keragaman dan kemerataan pada bangkai mencit (Mus musculus) yang diletakkan di darat dan di air serta perbedaan proses dekomposisi pada kedua lokasi. Hasil penelitian menunjukkan bahwa lalat pada bangkai mencit yang diletakkan di darat ditemukan sebanyak 135 individu yang terdiri dari tiga spesies yaitu Sarcophaga sp., Chrysomya megacephala, dan Musca domestica, sedangkan lalat yang ditemukan pada bangkai yang diletakkan di air sebanyak 9 individu terdiri dari tiga spesies yaitu Sarcophaga sp., Musca domestica, dan Fanniia sp. Hasil perhitungan indeks Shannon-Wienner komposisi lalat pada bangkai yang diletakkan di darat sebesar 0,44 dan pada bangkai yang diletakkan di air sebesar 0,68, sehingga dapat disimpulkan bahwa keragaman dan kelimpahan spesies lalat pada kedua lokasi bangkai tergolong rendah. Nilai indeks Eveness komposisi lalat pada bangkai yang diletakkan di darat sebesar 0,40 dan bangkai di air sebesar 0,62 maka dapat disimpulkan bahwa sebaran lalat pada bangkai di air lebih merata dan seragam dibandingkan dengan sebaran lalat pada bangkai di darat. Hasil paired t test menunjukkan bahwa peran lalat sebagai fragmenter bangkai mencit pada kedua lokasi sama baik dan menunjukkan bahwa tidak ada korelasi jumlah lalat di antara dua kondisi lingkungan tersebut (P>0,05), serta hasil analisis korelasi kedua komposisi lalat menunjukkan bahwa peran lalat sebagai fragmenter mencapai 47,4%. Proses dekomposisi bangkai di darat lebih cepat mencapai tahap akhir yaitu skeletal stage yang mulai terjadi pada hari kesepuluh pengamatan, sedangkan proses dekomposisi di air membutuhkan waktu yang lebih lama untuk mencapai tahap akhir yaitu sunken remains.
A Reproductive Performance of Black Soldier Fly (Hermetia illucens) With Pennisetum purpureum Extract Rifai, Lutfin Agri; Haryanto, Trisno; Ambarningrum, Trisnowati Budi; Hashifah, Fathimah Nurfitri; -, Mutala'liah
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 6 No 3 (2024): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2024.6.3.10560

Abstract

Black soldier fly (Hermetia illucens) is one of the biological agents for organic waste decomposition and this process produced insect biomass with significant economic value. Giving of juvenile hormone (JH) which is thought to be present in nappier grass (Pennisetum purpureum) can increase the number of BSF egg production. The purpose of this research is to determine the effect of nappier grass extract on the reproductive performance of H. illucens based on the number of eggs produced, the percentage of eggs hatched, and the sex ratio of BSF flies. The research was conducted experimentally using a completely randomized design (CRD). The number of eggs produced and the percentage of eggs hatched analyzed using non-parametric analysis with the Kruskal-wallis test and followed by the Mann Whitney test. Statistical testing using SPSS version 16.0. The sex ratio of BSF flies was analyzed descriptively. Phytochemical test analysis was carried out to ensure the content of compounds present in nappier grass extract (P. purpureum). The result shows that nappier grass extract has significant effect (P<0.05) on number of eggs produced and the percentage of eggs hatched based on Kruskal-wallis test. Based on the results of the Mann Whitney test, it was found that all treatments of nappier grass extract on the number of BSF egg production and the percentage of eggs hatched was significantly different (P<0.05). The highest egg production effect which the average number of egg production was 209 mg at 600 ppm. At 600 ppm had the highest percentage egg that hatched effect which the average of percentage egg that hatched was 88,759%. The optimum sex ratio for the cultivation was at 200 ppm, which is 60.42% female and 39.58% male Keyword: . Black Soldier Fly, Pennisetum purpureum, Reproductive performance
Co-Authors -, Mutala'liah Aliefia, Rizky Arjunnajat Alvira Rifdah Sativa Annisa, Dyah Retno Arif Rahman Hikam Atang Atang Atang, Atang Bambang Heru Budianto Darsono Darsono Dwi Iva Fitriana Dwi Sarwani Sri Rejeki Dwiana Muflihah Yulianti Dyah Fitri Kusharyati Dyah Retno Annisa Edi Basuki Edi Basuki Edi Basuki Edy Riwidiharso Emyliana Listiowati, Emyliana Endang Srimurni Kusmintarsih Endang Srimurni Kusmintarsih ENDANG SRIMURNI KUSMINTARSIH ENDANG SRIMURNI KUSMINTARSIH ENDANG SRIMURNI KUSMINTARSIH Femilia Hajar Ilhami Fitriana, Dwi Iva Haryanto, Trisno Hashifah, Fathimah Nurfithri Hashifah, Fathimah Nurfitri Hery Pratiknyo Hery Pratiknyo Hery Pratiknyo Hilmiyah Al'alawiyah Ika Rakhmayani Ilhami, Femilia Hajar Intan Ahmad Irma Fatmanintyas Irwan Susanto Khafida, Wilda Kustiati Kustiati Maula, Riska Isna Muslihah, Niken Istikhari Nirwani, Shintya Nitra Nuranisah, Annanda Pamudji, Ganjar Rakhmayani, Ika Rifai, Lutfin Agri Riska Isna Maula Rizky Arjunnajat Aliefia Rokhmani Rokhmani Sativa, Alvira Rifdah Setiyono, Eko Setyanto, Refius Pradipta Setyawati, Endang Ariyani Sri Lestari Sri Lestari Sri Yusmalinar Taruna Dwi Satwika Untung Susilo Yerslin Mantolu