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Journal : Biocelebes

UJI DAYA HAMBAT EKSTRAK BATANG TUMBUHAN Harrisonia perforata Merr. TERHADAP PERTUMBUHAN BAKTERI Shigella dysentriae Dewi Permatasari; Ramadhanil Ramadhanil; Syariful Anam; Ivan Ivan
Biocelebes Vol. 9 No. 1 (2015)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

The research entitled inhibition test of stem extract of Harrisonia perforata Merr. on the growth of bacteria Shigella dysentriae has been conducted from April - May 2014. The objective of the research was to study the inhibition stem extract of H. perforate Merr. on the bactery studied. The extraction was used reflux method. The experiment was arranged in Completely Randomized Designed (CRD) by 4 treatments (concentrate extract 60%, 80%, 100% and Contrimoxazale 1% as positive control) and 3 repetitions. The result showed that the best of extract concentrate to inhibite of bacterial growth was 100%. The treatment by 60% and 80% were not significant different statistically. Keywords : Harrisonia perforata Merr.,  Shigella dysenriae, Extract concentrate, Inhibition zone.
UJI DAYA HAMBAT DAUN Harrisonia perforata Merr. TERHADAP PERTUMBUHAN BAKTERI Escherichia coli DAN Salmonella typhi Irna Olvaliani Aimang; Ramadhanil Ramadhanil; Syariful Anam; Ivan Ivan
Biocelebes Vol. 9 No. 1 (2015)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

Reseacrh of inhibition test of leaves extract of Harrisonia perforata Merr. against to the growth of Eschericia coli and Salmonella thypi had been conducted during period of March to May 2014. The aim of this research was to gain the effectivity of leaves extract to inhibite the growth of E. coli dan S.thypi. Maceration method was used as the extraction method and Agar Diffusion method. The research was design by Completely Randomized Design with four(4) treatments and three(3) replications (for both bacterias). The treatments were leaves extract concentrate 15% 10%, 15%, and positive control (2 mg of Ciprofloxacin for E.coli and 2 mg Cloramfenikol for S.thypi). The result showed that leaves extract of H. perforata Merr. had inhibition effect to the growth of bacterias. The extract concentrate 15% produced the biggest inhibition zone for both E. coli and S. thypi (20,9 mm and 19,3 mm repectively) than other concentrates.   Keywords : Extract concentration, Harrisonia perforata Merr., Bacterial inhibition zone, Eschericia coli, Salmonella thypi.