Article Per Year (5 Year)
p-Index From 2021 - 2026
1.431
P-Index
This Author published in this journals
All Journal Molecular and Cellular Biomedical Sciences (MCBS) Jurnal Ilmu Kefarmasian Indonesia Majalah Patologi Indonesia Journal of Stem Cell Research and Tissue Engineering Nommensen Journal of Medicine Makara Journal of Health Research Cerdika: Jurnal Ilmiah Indonesia Journal of Global Pharma Technology
Jeanne Adiwinata Pawitan
Department Of Histology, Faculty Of Medicine, Universitas Indonesia
Humanities Biochemistry, Genetics & Molecular Biology Dentistry Economics, Econometrics & Finance Education Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health Social Sciences Veterinary

Published : 11 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search
Journal : Journal of Global Pharma Technology

 1 
Prospect of umbilical cord mesenchymal stem cell culture waste in regenerative medicine Jeanne Adiwinata Pawitan; Melisa Leviana; Dewi Sukmawati; Isabella Kurnia Liem; Ria Margiana; Twidy Tarcisia
Journal of Global Pharma Technology Volume 9 Issue 07
Publisher : Journal of Global Pharma Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Abstract: Objective:to analyzevarious growth factor (GF) content in umbilical cord derived mesenchymal stem cell (UC-MSC) culture waste at harvest.Methods: We cultured UC- MSCs in complete medium, and collected the medium waste at harvests.  We noted the time of harvests, and measured the concentrations of EGF, VEGF, NGF and Pl GF in the various medium wastes using ELISA method. We grouped GF concentrations according to various culture lengthsat harvest, and calculated themean values and standard deviations of the various GF. We compareddescriptively the GF concentrations at various culture lengths at harvests to conclude which condition yielded the overall highest GF concentration.Results: EGF, VEGF and PlGF concentrations were highest at harvest day-3, whileNGF concentration was the highest at harvest-day-5, respectively. Therefore, overall highest concentration of most GF was at harvest day 3.Conclusion:UC-MSC culture waste at harvest day-3 containedEGF, VEGF, NGF and PlGF, which might be used in regenerative medicine.
PASSAGE INFLUENCE ON THE AGING PROFILE AFTER SUCCESSIVE CRYOPRESERVATION OF BONE MARROW MESENCHYMAL STEM CELLS Ismail Hadisoebroto Dilogo; Starifulkani Arif; Jeanne Adiwinata Pawitan; Ria Anggraeni; Yogi Ismail Gani
Journal of Global Pharma Technology Volume. 9 Issue 6
Publisher : Journal of Global Pharma Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Objective: Bone marrow mesenchymal stem cells are promising in cell therapy but the low frequency of this subpopulation necessitates their in vitro expansion and cryopreservation prior to clinical use. The aim of this study was to analyze the passage effect on the aging profile of previously successive cryopreserved bone marrow mesenchymal stem cells.  Methods: The samples of this in-vitro observational analytic study (April - September 2016) were iliac crest bone marrow mesenchymal stem cells that were cryopreserved in passage one and a second group was cryopreserved after being subcultured. It was obtained from UPT TK Sel Punca RSCM-FKUI. Samples were analyzed for each passage in terms of cell size, viability, population doubling time (PDT), and percentage of senescent cells by ANOVA test and independent t-test for the inter-cryopreservation group.Results: The remarkable manifestation of senescence appeared at passage six in both cryopreserved groups, but tend to appear earlier in the twice cryopreservation group. The senescent positive cell size became bigger along with the increasing number of passage. There were significant differences in PDT, 30% confluent cell size, viability, in passage six between the two cryopreservation groups (p<0.001, p<0.001, p=0.022), respectively, but no significant difference in percentage of senescent cells (p= 0.052). The cells became senescent in P6 in both groups (P=0.000).Conclusion: The onset of senescence of bone marrow mesenchymal stem cells was in passage six, while PDT, cell size and viability differ significantly due to successive cryopreservation.
Co-Authors Ahmad Aulia Antarianto, Radiana Barasila, Atikah Chalida Budiman Bela CAROLINE TAN SARDJONO Christine Verawaty Sibuea Cosphiadi Irawan Des Suryani Dewi Sukmawati Dian Ratih Laksmitawati Ecie Budiyanti Fasha, Iqbal Ismail Hadisoebroto Dilogo Jonlean, Reganedgary K Kartini Kousar, Iqra Liem, Isabella Kurnia Mahmood, Amer Melinda Remelia Melisa Leviana Melva Louisa Mohamad Sadikin Morioka, Hiroshi Murdani Abdullah Nishikawa, Satoshi Noza Hilbertina Noza Hilbertina, Noza Nuzli Fahdia Mazfufah Oktarina, Caroline Purwoko, Reza Yuridian Radiana Dhewayani Antarianto Rahyussalim Rahyussalim, Rahyussalim Retno Wilujeng Susilowati Ria Anggraeni Ria Margiana Silvia Tri Widyaningtyas Starifulkani Arif Tandarto, Kevin Twidy Tarcisia Uesugi, Seiichi Wahyunia Likhayati Septiana Yogi Ismail Gani