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Journal : The Indonesian Biomedical Journal

Ethanol Extract of Spondias pinnata Leaves Reduce Parasite Number and Increase Macrophage Phagocytosis Capacity of Mice Infected by Plasmodium berghei Dewa Ayu Agus Sri Laksemi; I Gusti Kamasan Arijana; I Made Sudarmaja; Ni Luh Ariwati; Ketut Tunas; Putu Ayu Asri Damayanti; Ni Luh Putu Eka Diarthini; I Kadek Swastika; Ida Ayu Dewi Wiryantini
The Indonesian Biomedical Journal Vol 13, No 1 (2021)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v13i1.1286

Abstract

BACKGROUND: Currently, there is no vaccine against malaria in humans, the development of resistance to anti-malarial drugs, causing the need to find new alternatives to overcome malaria infections. This study aimed to determine effect of Spondias pinnata in increasing cellular immunity, especially phagocytosis activity of peritoneal macrophages against Plasmodium berghei infection.METHODS: This was an experimental study with two stages of research, each stage requires 36 Balb/c mice, aged 2 months and weight 20-25 grams. After one week of acclimatization, the mice were put into 6 different groups, each group consisted of 6 mice. The negative control was a group of mice given distilled water for 14 days then infected by P. berghei in the 15th day. Meanwhile, T1, T2, T3, T4 and T5 groups were given S. pinnata leaves ethanol extract with dose of 25, 50, 100, 200 and 400 mg/kg body weight (BW)/day, respectively, and then infected by P. berghei in the 15th day.RESULTS: The results showed that the lowest parasitemia and the highest capacity of macrophage to phagocytose latex was found in treatment group T3 that received 50 mg/kg BW of S. pinnata leaves ethanol extract. Based on analysis of the Pearson correlation test, there was a significant correlation between percent phagocytosis and parasitemia (p<0.05).CONCLUSION: Ethanol extract of S. pinnata leaves lower the parasite number of P. berghei in Balb/c mice and increase the capacity of macrophage to phagocytose latex. However, the mechanisms of how S. pinnata leaves extract in activating phagocytosis capacity and reducing parasitemia still need further investigation.KEYWORDS: phagocytosis, Plasmodium berghei, parasite number, Spondias pinnata
Serum DNase1, sTNFR1 and sTNFR2 as Risk Factors for Lupus Nephritis in Systemic Lupus Erythematosus Patients Manuaba, Ida Ayu Ratih Wulansari; Suryana, Ketut; Bakta, I Made; Sudarmaja, I Made
The Indonesian Biomedical Journal Vol 16, No 3 (2024)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v16i3.3052

Abstract

BACKGROUND: Early detection and management of lupus nephritis (LN) in systemic lupus erythematosus (SLE) are essential to prevent irreversible kidney damage and improve patient outcomes; therefore, identifying reliable biomarkers to predict LN is paramount. However, there are still relatively few studies examining the potential biomarkers for LN in SLE patients. This study was conducted to investigate serum deoxyribonuclease I (DNase), soluble tumor necrosing factor 1 (sTNFR1) and soluble tumor necrosing factor 2 (sTNFR2) as a risk factor for LN in SLE patients.METHODS: A case-control study involving SLE patients aged 20-60 years was conducted. Blood was withdrawn from each subject for the measurement of serum level of DNase1, sTNFR1, and sTNFR2 that was performed using enzyme-linked immunoassay (ELISA) methods. Data was then analyzed using Chi-Square test and logistic regression tests.RESULTS: A total of 22 patients with LN and 22 without LN were included. The cut-off value for DNase1, sTNFR1, and sTNFR2 were 5.05 ng/mL, 6.52 ng/mL, and 7.02 ng/mL, respectively. The risk factors of LN in SLE patients were the low level of serum DNase1 (aOR=6.64; 95%CI: 1.25-35.29; p=0.026), low level of serum sTNFR1 (aOR=8.12; 95% CI: 1.56-42.10; p=0.013), and low level of serum sTNFR2 (aOR=5.57; 95%CI: 1.03-30.11; p=0.046).CONCLUSION: Serum DNase1 lower than 5.05 ng/mL, sTNFR1 lower than 6.52 ng/mL, and sTNFR2 lower than 7.02 ng/mL were risk factors for lupus nephritis in SLE patients. Hence, serum DNase1, sTNFR1 and sTNFR2 could be used as risk factors predictors for LN in SLE patients.KEYWORDS: DNase1, sTNFR1, sTNFR2, SLE, lupus nephritis
Co-Authors A.A Indah Suadnyani A.A Indah Suadnyani Abdulhadi FA Adiputra, I Komang Hotra Agilan Sethupathy Amaral, Meriana Barreto Amelia, Irene Anak Agung Ayu Mirah Adi Ariwati, Ni Luh Ayu Surasmiati, Ni Made Bagus Komang Satriyasa Cokorda Agung Wahyu Purnamasidhi Daondy Friarsa Soeharto Dewa Ayu Agus Sri Laksemi Dewa Ayu Agus Sri Laksmi Divya Nirmala, Putu Ayu Edwin Adrianta Surijah Eka Sutyawan, I Wayan I Gusti Agung Ayu Chintya Cahyarini I Gusti Agung Dwi Putri Anjani I Gusti Ayu Prapti Adnyani I Gusti Ayu Prapti Adnyani I Gusti Kamasan Arijana I Kadek Swastika I Ketut Tunas I Made Bakta I Made Jawi I NYOMAN MANTIK ASTAWA I Putu Dema Prasetya I Putu Dodik Supartha I Wayan Ardyan Sudharta Putra I Wayan Putu Sutirta Yasa I Wayan Sugiritama Ida Ayu Dewi Wiryantini Ida Ayu Ide Larassanthi Pratiwi Ida Ayu Sintya Pratiwi Ida Bagus Putra Manuaba Ivy Cerelia Valerie Kadek Adit Wiryadana Kadek Yuda Dira Pratama Ketut Suryana Ketut Teddy Bayu Pradinata kumara, i made bagus bayu L. Ariwati Luh Ariwati Luh Putu Ratna Sundari Lusi Martha Indrayani Made Lady Adelaida Made Martha Pradnyana Made Sushmita Dharmasuari Maretta Rosabella Purnamasari Maretta Rosabella Purnamasari Maria Krishnandita N.L Ariwati N.L.P.E. Diarthini Ni Luh Ariwati Ni Luh Ariwati Ni Luh Jayanti Wulan Sari Ni Luh Komang Sumi Arcani Ni Luh Putu Eka Diarthini Ni Luh Putu Eka Diarthini, Ni Luh Putu Eka Ni Luh Rustini Ni Made Laksmi Utari Ni Made Linawati Ni Made Suasti Wulanyani Ni Nyoman Agustianingsih Ni Putu Akopita Devi Ni Putu Tamara Bidari Suweta Nurulhuda Binti Embong P.A.A. Damayanti Pandango, Ivana Griselarosa Koni Pratisthita Sukadana, I Made Ananda Prajna Putu Ari Paramitha Widiani Putu Ayu Asri Damayanti Putu Ayu Larasati Putu Bagus Onicha Baskaranatha Putu Diah Saraswati Rahayu Putu Indah Budi Apsari REFAYANTI, NI MADE ERNITA Saraswati Devi, Ni Made Kumbha Mella Shabrina Inderjit Sri Laksemi, Dewa Ayu Agus SugengO Juwono Mardihusodo Widiyanti, I Gusti Ayu Wulansari Manuaba, Ida Ayu Ratih Wulanyani Wulanyani