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Journal : Journal of Global Pharma Technology

Comparison of the effects of ethanol extract with aqueous extract of purple sweet potato tuber in expression of SOD-2, SOD-3, and eNOS on human vascular endothelial cells exposed by H2O2 in-Vitro I Made Jawi; I G K Arijana; I Wayan Putu Sutirta Yasa; AAN Subawa
Journal of Global Pharma Technology .
Publisher : Journal of Global Pharma Technology

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Abstract

Aqueous extract of purple sweet potato tubers have antioxidant potential in animal and human vascular endothelial, through increased expression of superoxide dismutase. This study aims to prove the comparison between ethanol extract and aqueous extract of purple sweet potato tubers increasing the expression of superoxide dismutase -2, superoxide dismutase -3, and endothelial nitric oxide synthase in human vascular endothelium. This study was an experimental study of the vascular endothelial cells which underwent oxidative stress in vitro, which is protected by ethanol extract or aqueous extract of purple sweet potato tuber at various concentrations. Observation of the superoxide dismutase -2, superoxide dismutase -3, and endothelial nitric oxide synthase in endothelial cells is done using the method immunohistochemistry with monoclonal antibodies. The results showed an increase in superoxide dismutase - 2, superoxide dismutase -3, and endothelial nitric oxide synthase was significantly (p < 0.05) in endothelium protected by ethanol extract or water extract of purple sweet potato tubers. The ethanol extract only showed its effect at concentration of 1.5625 µg/ml for superoxide dismutase -2 and superoxide dismutase -3. The effect of aqueous extract were at a concentration of 1.5625–3.125µg/ml. From these results it can be concluded that the ethanol extract or aqueous extract of purple sweet potato tubers can protect endothelial cells from oxidative stress through increased expression of superoxide dismutase -2 and superoxide dismutase -3. The ethanol extract requires much lower concentrations to show its effect compared with aqueous extracts. Keywords: Aqueous extract or ethanol  extract of purple sweet potato, Superoxide dismutase, Human umbilical vein endothelial cells.
Co-Authors ADNYANA PUTRA, I GEDE AGUS DIVA Agus Dody Pranata Suadi Putra Anak Agung Ngurah Subawa Apolonia Berenika Badu Ayu Gita Bhagawanti Ayu Saka Laksmita W D.A.A.S. Laksmi Desak Putu Citra Udiyani Desak Putu Risky Vidika Apriyanthi Dewa Ayu Agus Sri Laksmi Dinesh Tanabbal ELVINA VERONICA Eugenia Meidy Fedik Abdul Ratam Gayathiri Mohana Krishnan Gede Wirata Hamsu Kadriyan Henky Henky I Dewa Ayu Eka Widiari Putri I Dewa Ayu Inten Primayanti I Dewa Made Agus Paramarta Putra I Gde Raka Widiana I Gusti Ayu Dewi Ratnayanthi I Gusti Ayu Dewi Ratnayanti I Gusti Kamasan I Gusti Ngurah Mayun I Gusti Nyoman Sri Wiryawan I Kadek Swastika I Ketut Tunas I Made Adi Satria Darma I Made Agus Endra Permana I Made Jawi I Made Sudarmaja I Nyoman Hariyasa Sanjaya I Nyoman Wande I Nyoman Wiryawan I Putu Dema Prasetya I Wayan Gede Artawan Eka Putra I Wayan Putu Sutirta Yasa I Wayan Sugiritama I Wayan Sugiritama I. G. A. D. Ratnayanthi I.G.N.S. Wiryawan I.N. Wande I.W. Sugiritama Ida Ayu Dewi Wiryantini Ida Ayu Ika Wahyuniari Intan Syahirah Bt Abdul Rauap JOHAN AXEL PARIURY Joshua Ezra Ronaldo Bayak JUAN PAUL CHRISTIAN HERMAN Juven Luvianto Komang Jegek Triangga Apsari Made Satria Yudha Dewangga Mahen Isaac Pannir Chelvam N. M. Linawati N.M. Linawati Ni Ketut Susilawati Ni Luh Ariwati Ni Luh Putu Eka Diarthini Ni Made Linawati Ni Made Mertaniasih Ni Made Yuli Lestari Ni Nyoman Mas Utari Rena Ni Nyoman Sri Budayanti Ni Putu Candra Paramita Ni Putu Sriwidyani Ni Putu Widayanti Ni Wayan Devi Yulianti Nyoman Intan Cahaya Pertiwi Nyoman Pratiwi Hapsari Dewi P.P.A.P. Dewi Putu Ayu Asri Damayanti Raymond Elbert Budianto Romdhoni, Achmad Chusnu Ruthirar Kalaichelvam Sri Maliawan Tarvin Jit Singh Gill TIFFANY REBECCA Tjokorda Gde Agung Suwardewa Wayan Aryadana Wayan Aryadana Wayan Suardana Yogarani V.C Rajappan