Endang Supriyantini
Departement Of Marine Science, Faculty Of Fisheries And Marine Sciences Diponegoro University Jl. Prof. H. Soedarto, S.H, Tembalang Semarang. 50275 Telp/fax (024)7474698

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Bioenkapsulasi Naupli Artemia dengan Spirulina sp. dan Resistensinya terhadap Bakteri Vibrio spp. Sri Sedjati; Ervia Yudiati; Endang Supriyantini; Nuril Azhar; Chika Velita Anindya Yulian
Jurnal Kelautan Tropis Vol 25, No 1 (2022): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v25i1.12763

Abstract

Artemia is usually given as live feed in shrimp and fish hatcheries. Bioencapsulation of Artemia with Spirulina sp. is thought to increase disease resistance. One disease that often harms aquaculture is infection by Vibrio. The study aimed to determine the significance of artemia's nauplii biocapsulation to increased resistance against infection by Vibrio spp. The research method used is experimental laboratories with a non-parametric approach. The resistance variable is measured as the survival rate. Bioencapsulation of Artemia uses 4 levels of Spirulina sp. concentration: 0 (control), 300, 600, 900 mg/L, while the challenge test uses 8 levels of Vibrio: Non-Vibrio (control), Vibrio vulnificus (Vv), V. harveyi (Vh), V. parahaemolyticus (Vp), a combination of the two: (Vv+Vh), (Vv+Vp), (Vh+Vp), as well as a combination of the three: (Vh+Vp+Vv). Kruskal Wallis test was conducted to find out the difference in artemia survival rates between groups, then the U Mann-Whitney test to find out pairs between groups that have significant differences. Kruskal Wallis analysis showed only spirulina sp concentrations that produce a significant effect (p=0.00) on the survival rate of nauplii Artemia, while Vibrio spp. have no effect (p=0.32). Based on the U Mann-Whitney test, all spirulina sp concentration groups are significantly different from control (p<0.05), and only the group 300 to 600 mg/L is not different (p=0.42). Spirulina sp. 300 mg/L can increase survival rates to 77.92%, 600 mg/L to 77.50%, and 900 mg/L to 66.67%. In conclusion, it is optimal to increase Artemia's resistance to Vibrio spp. can be done with bioencapsulation of Spirulina sp. 300 mg/L.  Artemia biasa diberikan sebagai pakan hidup pada usaha pembenihan udang maupun ikan. Bioenkapsulasi Artemia dengan Spirulina sp. diduga dapat meningkatkan resistensi terhadap penyakit. Salah satu penyakit yang sering merugikan usaha akuakultur adalah infeksi oleh bakteri Vibrio. Tujuan dari penelitian ini adalah untuk melihat signifikansi bioenkapsulasi naupli Artemia terhadap peningkatan resistensi melawan infeksi oleh Vibrio spp. Metode penelitian yang digunakan adalah eksperimental laboratoris dengan pendekatan non-parametrik. Variabel resistensi diukur sebagai tingkat kelangsungan hidup. Bioenkapsulasi Artemia menggunakan 4 taraf konsentrasi Spirulina sp.: 0 (kontrol), 300, 600, 900 mg/L,sedangkan uji tantang menggunakan 8 taraf jenis Vibrio: Non-Vibrio (kontrol), Vibrio vulnificus (Vv), V. harveyi (Vh), V. parahaemolyticus (Vp), kombinasi dari dua Vibrio: (Vv+Vh), (Vv+Vp), (Vh+Vp), serta kombinasi dari ketiganya: (Vh+Vp+Vv). Uji Kruskal Wallis dilakukan untuk mengetahui perbedaan tingkat kelangsungan hidup Artemia antar kelompok, selanjutnya dilakukan uji U Mann-Whitney untuk mengetahui pasangan antar kelompok yang memiliki perbedaan signifikan. Hasil analisis Kruskal Wallis menunjukkan bahwa hanya konsentrasi Spirulina sp. yang menghasilkan pengaruh signifikan (p=0,00) terhadap tingkat kelangsungan hidup naupli Artemia, sedangkan jenis Vibrio spp. tidak berpengaruh (p=0,32). Berdasarkan uji U Mann-Whitney, semua kelompok konsentrasi Spirulina sp berbeda secara signifikan terhadap kontrol (p<0,05), dan hanya kelompok. 300 terhadap 600 mg/Lyang tidak berbeda (p=0,42). Pemberian Spirulina sp. sebanyak 300 mg/Ldapat meningkatkan tingkat kelangsungan hidup menjadi 77,92%, 600 mg/Lmenjadi 77,50%, dan 900 mg/Lmenjadi 66,67%. Kesimpulannya, secara optimum untuk meningkatkan resistensi Artemia terhadap Vibrio spp. dapat dilakukan dengan bioenkapsulasi Spirulina sp. 300 mg/L. 
Pemanfaatan Chitosan Dari Limbah Cangkang Rajungan (Portunus pelagicus) sebagai Adsorben Logam Timbal (Pb) Endang Supriyantini; Bambang Yulianto; Ali Ridlo; Sri Sedjati; Amtoni Caesario Nainggolan
Jurnal Kelautan Tropis Vol 21, No 1 (2018): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (610.261 KB) | DOI: 10.14710/jkt.v21i1.2399

Abstract

Chitosan is able to be one solution to reducing the pollution of heavy metals in waters, as capable of binding of heavy metal ions by utilizing the amine and hydroxyl groups on chitosan. This research aims to know the adsorption ability of chitosan against heavy metals lead (Pb). Chitosan is made from the waste of a small crab attaching shells were taken from the village of Betahwalang, Demak. The method used is the method with the demineralisasi using 1 N HCl for 1 hour, deproteinasi with 3.5% NaOH for 1 h, and deasetilasi using NaOH 50% for 2 hours. FTIR analysis of the results showed the degree of Deasetilasi Chitosan of 80.30% with 36.84% yield. Solution of Pb of Pb (CH3COO)2 at a concentration of 10 mg/L is used as pollutants, and the concentration of chitosan used in adsorption process i.e. 1, 2, 4, and 8%. Stirring on the process of adsorption using a magnetic stirrer with a speed of 200 rpm for 30 minutes. The metal content of Pb in the chitosan is then analyzed using AAS (Atomic Absorption Spectrophotometric). The results showed that chitosan obtained from shell small crab attaching has the potential as a bioadsorben metals Pb, the concentration of chitosan 1, 2, 4, 8% & each have an absorbance of 8.31; 35.62; 45.24 & 57.74%.Kitosan mampu menjadi salah satu solusi mengurangi pencemaran logam berat di perairan, karena mampu mengikat ion-ion logam berat dengan memanfaatkan gugus hidroksil dan amina yang terdapat pada chitosan. Penelitian ini bertujuan untuk mengetahui kemampuan adsorpsi chitosan terhadap logam berat Timbal (Pb). Chitosan dibuat dari limbah cangkang rajungan yang diambil dari desa Betahwalang, Demak. Metode yang digunakan adalah metode kimia dengan demineralisasi menggunakan HCl 1 N selama 1 jam, deproteinasi dengan NaOH 3,5 % selama 1 jam, dan deasetilasi menggunakan NaOH 50 % selama 2 jam. Hasil analisis FTIR menunjukkan Derajat Deasetilasi kitosan sebesar 80,30 % dengan rendemen 36,84 %. Larutan Pb dari Pb(CH3COO)2 pada konsentrasi 10 mg/L digunakan sebagai polutan, dan konsentrasi chitosan yang digunakan pada proses adsorpsi yaitu 1, 2, 4, dan 8 %. Pengadukan pada proses adsorpsi menggunakan magnetic stirrer dengan kecepatan 200 rpm selama 30 menit. Kandungan logam Pb yang ada di dalam chitosan kemudian dianalisis dengan menggunakan metode AAS (Atomic Absorption Spectrophotometric). Hasil penelitian menunjukkan bahwa chitosan yang didapatkan dari cangkang rajungan mempunyai potensi sebagai bioadsorben logam Pb, konsentrasi chitosan 1, 2, 4, & 8 % masing-masing memiliki daya serap sebesar 8,31; 35,62 ; 45,24 & 57,74 %.
Daya Serap Mangrove Rhizophora sp. Terhadap Logam Berat Timbal (Pb) Di Perairan Mangrove Park, Pekalongan Endang Supriyantini; Ria Azizah Tri Nuraini; Cintya Pramesthi Dewi
Jurnal Kelautan Tropis Vol 20, No 1 (2017): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (491.48 KB) | DOI: 10.14710/jkt.v20i1.1349

Abstract

Human activities in the Mangrove Park waters, Pekalongan area can  be increasing the present of heavy metal in acuatiq environmental and aquatic organisms. The purpose of this research is (1) to know the heavy metal content of Pb in water, sediment, root and mangrove leaves Rhizophora sp; (2) to know the level of Pb heavy metal pollution in Mangrove Park area; (3) Bioconcentration factor (BCF) on root and Rhizophora sp.This research was conducted in November 2015 and January 2016. The materials used in this study are water samples, sediments, roots, and leaves Rhizophora sp. The method of determining the location of research stations based on Purposive Sampling Method. Analyze heavy metal Pb using AAS (Atomic Absorption Spectrophotometry) at the Laboratory of Center for Industrial Pollution Prevention Technology (BBTPPI). The results showed that in Mangrove Park waters, Pekalongan has not polluted Pb metal since it has not exceeded the determined threshold limit. The content of Pb in sediment is still below the specified quality standard of 12.06-14.77 mg / kg (November 2015) and 10.65-13.26 mg / kg (January 2016). The content of Pb at the root is 0.25-0.32 mg / kg (November 2015) and 0.15-0.47 mg / kg (January 2016). The content of Pb metal on leaves is 0.17-0.22 mg / kg (November 2015) and 0.17-0.59 mg / kg (January 2016). Based on the bioconcentration factor, the ability of Rhizophora sp. In accumulating Pb metals is low.  Kegiatan manusia di kawasan Perairan Mangrove Park, Pekalongan dapat meningkatkan kehadiran logam berat di lingkungan perairan dan organisme air. Tujuan penelitian ini adalah (1) untuk mengetahui kandungan logam berat Pb dalam air, sedimen, akar dan daun mangrove Rhizophora sp. di kawasan Mangrove Park Pekalongan;(2) mengetahui tingkat pencemaran logam berat Pb di kawasan Mangrove Park Pekalongan; (3) Mengetahui Biokonsentrasi Faktor (BCF) pada akar dan daun Rhizophora sp. Penelitian dilakukan pada bulan November 2015 dan Januari 2016. Materi yang digunakan untuk penelitian adalah sampel air, sedimen, akar, dan daun Rhizophora sp. Metode penentuan lokasi penelitian berdasarkan Purposive Sampling Method. Analisis logam berat Pb menggunakan AAS (Atomic Absorption Spectrophotometry) di Laboratorium Balai Besar Teknologi Pencegahan Pencemaran Industri (BBTPPI). Hasil penelitian menunjukkan di perairan Mangrove Park, Pekalongan belum tercemar logam Pb karena belum melebihi batas ambang yang ditentukan. Kandungan Pb dalam sedimen masih dibawah baku mutu yang ditentukan yaitu 12.06-14.77 mg/kg (November 2015) dan 10.65-13.26 mg/kg (Januari 2016). Kandungan Pb pada akar sebesar 0.25-0.32 mg/kg (November 2015) dan 0.15-0.47 mg/kg (Januari 2016). Kandungan logam Pb pada daun 0.17-0.22 mg/kg (November 2015) dan 0.17-0.59 mg/kg (Januari 2016). Berdasarkan factor biokonsentrasi, kemampuan Rhizophora sp. dalam mengakumulasi logam Pb tergolong rendah.  
Aktivitas Antioksidan dan Kandungan Senyawa Fenolik Makroalga Coklat Sargassum sp. Sri Sedjati; Suryono Suryono; Adi Santosa; Endang Supriyantini; Ali Ridlo
Jurnal Kelautan Tropis Vol 20, No 2 (2017): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (496.632 KB) | DOI: 10.14710/jkt.v20i2.1737

Abstract

Present research was to evaluated the antioxidant activities of extracts from macroalga Sargassum sp.  In this study, seven filtrates from maceration (methanol pa and technical grade, ethanol pa and techinal grade, methanol technical grade 50%,  infused hot water, boiled water) were analyzed for total phenolic compound (TPC) and for antioxidant activities (DPPH method).  The results revealed that the water filtrates possessed more TPC and antioxidant antivity than alcoholic filtrates, and the highest was water filtrate from boiled Sargassum sp. (TPC =1,36±0,01 mgGAE/g  dry weight; % inhibtion DPPH = 81,35±0,42 %). The high positive correlation between TPC and antioxidant activity (r = 0.99 ) suggested that phenolic compound  were the mayor antioxidant components.  The effect of TPC (dependent variable) on DPPH radical inhibition (independent variable) with regression formula y = 55,39 x + 5,18.  Penelitian ini mengevaluasi potensial aktivitas antioksidan ekstrak Sargassum sp.  Pada kajian ini, 7 filtrat hasil maserasi (metanol pa dan teknis, etanol pa dan teknis, metanol teknis 50%, perendaman air panas/hot infused, dan perebusan dengan air) dianalisis kandungan total fenoliknya (TPC) dan aktivitas antioksidannya (metoda DPPH).  Hasil penelitian ini mengungkapkan bahwa filtrat air memiliki kandungan TPC dan antioksidan yang relatif lebih tinggi dibanding filtrat alkohol dan yang tertinggi adalah filtrat hasil perebusan Sargassum sp. (TPC =1,36±0,01 mgGAE/g  berat  kering;  inhibisi DPPH = 81,35±0,42 %),  Korelasi positif yang tinggi antara TPC dan aktivitas antioksidan (r = 0.99) menunjukkan bahwa  kandungan senyawa fenolik merupakan komponen utama yang menghasilkan  aktivitas  antioksidan.  Pengaruh  TPC (variabel bebas)  terhadap inhibisi radikal DPPH (variabel terikat)  sesuai dengan persamaan regresi  y = 55,39 x + 5,18.  
Kandungan Pigmen, Total Fenolik Dan Aktivitas Antioksidan Sargassum sp. Sri Sedjati; Endang Supriyantini; Ali Ridlo; Nirwani Soenardjo; Victorina Yulina Santi
Jurnal Kelautan Tropis Vol 21, No 2 (2018): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (594.309 KB) | DOI: 10.14710/jkt.v21i2.3329

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Pigmen content, total phenolic compound and antioxidant activity Sargassum sp.   Sargassum sp. contains secondary metabolites which potentially act as natural source of antioxidant. The purpose of this study was to determine bioactive contents (pigments, total phenolic compounds), and antioxidant activities of Sargassum sp. The method of this research is descriptive-explorative.  Sample was extracted with methanol, while pigments extraction with aseton 80%.  Chlorophyll a, b and carotenoids were carried out with spectrophotometer method. The total phenolic compounds were analyzed by spectrophotometer method using Follin-Ciocalteu reagent, and antioxidant activities were measured using DPPH (1,1-diphenyl-2- picrylhidrazyl) method. The result of this research showed that methanol extract of Sargassum sp. had the presence of chlorophyll a 2.84 mg/g, chlorophyll b 1.15 mg/g, and carotenoids 2.63 μmol/g sample. The total phenolic compounds of methanol extract were 57.97 mg GAE/g sample. Methanol extract of Sargassum sp. was showing a strong antioxidant activity that could be seen in IC50 value (69,27 ppm) in less than 100 ppm concentration. Sargassum sp. mengandung beberapa metabolit sekunder yang berpotensi sebagai sumber antioksidan alami. Tujuan dari penelitian ini adalah mengkaji komponen bioaktif (pigmen, total fenolik) dan aktivitas antioksidan dari rumput laut Sargassum sp. Metoda penelitian ini adalah diskriptif eksploratif.  Sampel Sargassum sp. basah diekstraksi dengan cara maserasi dengan pelarut  metanol, sedangkan ekstraksi pigmen menggunakan aseton 80%. Penentuan kadar pigmen (klorofil a, b, karotenoid ), total fenolik dan aktivitas antioksidan berdasarkan metode spektrofotometri. Total fenolik diukur berdasarkan uji Follin-Ciocalteu, dan   aktivitas antioksidan  berdasarkan uji DPPH (1,1-diphenyl-2- picrylhidrazyl). Hasil penelitian menunjukan bahwa ekstrak metanol Sargassum sp.  mengandung pigmen klorofil a sebesar 2,84 mg/g, klorofil b sebesar 1,15 mg/g, dan karotenoid sebesar 2,63 μmol/g. Kadar total fenolik ekstrak metanol adalah 57,97 mgGAE/g, sementara aktivitas antioksidannya  (IC50) sebesar 69,27 ppm.  Berdasarkan nilai IC50nya, ekstrak metanol Sargassum sp berpotensi sebagai antioksidan kuat (kurang dari 100 ppm).
Antimicrobial Activity of Fungal Extract of The Aspergillus flavus from Hiri Island, North Maluku to Pathogenic Bacteria Sri Sedjati; Ambariyanto Ambariyanto; Agus Trianto; Endang Supriyantini; Ali Ridlo; Muhammad S. Bahry; Rizky Rifatma Jezzi; Mahadika Fanindhita Sany
Jurnal Kelautan Tropis Vol 23, No 1 (2020): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v23i1.7049

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Antibacterial compounds from sponge association fungi are one of the alternatives to search for new antibiotics against resistant bacteria. This study aims to explore the secondary metabolites potential from sponge association fungi as MDR pathogens antibacterial and to cultivate these fungi using a variety of cultivation media. TE-BO-09.1. Isolate can inhibit 3 bacteria, K. pneumoniae, B subtilis, and S. aureus, but is not able to inhibit E. coli and P. aeruginosa. Cultivation using standard media of Malt Extract Agar (MEA) and media modified from fish broth (M1, M2) and cassava infusion (M3, M4) produce secondary metabolites with varying quantities and antimicrobial activity. The inhibition zone of the produced extract with 500 μg/disc concentration ranged from 7.14 to 10.32 mm. The strongest potential was shown by ethyl acetate extract from isolates cultured with M2 (9 days cultivation), of which is able to produce 10.32 mm inhibition zones against S. aureus and methanol extracts from isolates cultured with M4 (6 days cultivation), and able to produce 10.05 mm inhibition zones against K. pneumoniae. In conclusion, the fungus  Aspergillus flavus can be culture using fish broth added glucose and cassava infusion water added peptone media to potentially produce antibacterial compounds against MDR pathogens.
Analisis Kadar Tanin Dalam Buah Mangrove Avicennia marina Dengan Perebusan Dan Lama Perendaman Air Yang Berbeda Nirwani Soenardjo; Endang Supriyantini
Jurnal Kelautan Tropis Vol 20, No 2 (2017): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (454.072 KB) | DOI: 10.14710/jkt.v20i2.1701

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Mangrove fruit has the potential to be developed as a potential food source, but the presence of toxins in the fruit are considered harmful if consumed in the long run. Therefore, research needs to be done to eliminate or reduce the levels of toxins in the mangrove fruit, in order to increase the potential of the mangrove plants. The poison is in the mangrove fruit tannins. One alternative to reduce the levels of toxins in the fruit of the mangrove scrub with ash and water immersion.The purpose of this study was to determine the effect of treatment of ash powder content and the duration of water immersion on fruit tannin content of mangrove Avicennia.marina.The treatment was a long 24 hours of water immersion 4 times change of water (6 hours), 48 hours 8 times a change of water (6 hours), and 72 hours 12 times a change of water (6 hours). The results showed that treatment long soaking water to give a significant influence (P <0.05) toward decreased levels of mangrove Avicennia marina fruit tannins. Treatment a long 72 hours of water immersion 12 times a change of water (6 hours) to reduce levels of tannins by 28.80%.  Buah mangrove mempunyai potensi untuk dikembangkan menjadi sumber pangan yang potensial, akan tetapi dalam buahnya mengandung tannin . Tanin merupakan zat alami yang dihasilkan oleh buah mangrove yang memberi rasa getir. Oleh karena itu, perlu dilakukan penelitian untuk mengurangi kadar tannin dalam buah mangrove. Tujuan penelitian ini adalah untuk menganalisis pengaruh perlakuan  lama waktu perendaman air terhadap kadar tanin buah mangrove A.marina.Perlakuan  lama perendaman air 24 jam 4 kali pergantian air (6 jam), 48 jam 8 kali pergantian air (6 jam), dan 72 jam 12 kali pergantian air (6 jam). Hasil penelitian menunjukan bahwa perlakuan lama perendaman air memberikan pengaruh yang nyata (P < 0.05) terhadap penurunan kadar tanin buah mangrove Avicennia marina. Perlakuan lama perendaman air 72 jam 12 kali pergantian air (6 jam) menurunkan kadar tanin  sebesar 28,80 % 
Mikroplastik pada Kedalaman Sedimen yang Berbeda di Pantai Ayah Kebumen Jawa Tengah Ali Ridlo; Raden Ario; Arif Maa’ruf Al Ayyub; Endang Supriyantini; Sri Sedjati
Jurnal Kelautan Tropis Vol 23, No 3 (2020): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v23i3.7424

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Microplastics are one of the most significants pollutan in the marine environment and accumulate in sediments. The purpose of this research is to know the abundance of microplastics at different sediment depth in Ayah Beach Kebumen Central Java. The sediment samples were collected from three depth, 0-5 cm, 6–10 cm and 11–15 cm. microplastics were separated from sediment through density fracxination using NaCl solutiaon and ZnCl2 solution respectively. This study demonstrated that microplastics were consistenly found in all depth. The type of microplastics found were fiber, fragment and film. Fiber was the most frequent type (> 70%) in all samples. At 0–5 cm depth were found  578 microplastisc particle, at 6–10 cm depth were 459 particles, and at 11–15 cm depth were 610 particles.     Mikroplastik merupakan salah satu polutan penting di laut dan terakumulasi di sedimen.  Penelitian ini bertujuan untuk mengetahui kandungan mikroplastik di sedimen Pantai Ayah kebumen Jawa Tengah. Penelitian ini bertujuan untuk mengetahui kelimpahan mikroplastik pada kedalaman sedimen 0-5 cm, 6–10 cm dan 11–15 cm. Mikroplastik dipisahkan dari sedimen dengan cara fraksinasi berdasarkan densitasnya berturut-turut menggunakan larutan NaCl dan larutan ZnCl2. Hasil penelitian menunjukkan mikroplastik ditemukan pada setiap kedalaman. Bentuk mikroplastik yang ditemukan adalah fragmen, fiber dan film. Bentuk fiber menyusun lebih dari 70 % mikroplastik dalam sedimen di ketiga tingkat kedalaman. Pada kedalaman 0–5 cm terdapat 578 partikel mikroplastik, pada kedalaman 6–10 cm ditemukan 459 partikel, dan pada kedalaman 11–15 cm terdapat  610 partikel. 
Kandungan Total Fenolat pada Ekstrak Rhizophora sp Dari Teluk Awur, Jepara Ali Ridlo; Endang Supriyantini; Sri Sedjati
Jurnal Kelautan Tropis Vol 22, No 1 (2019): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (416.741 KB) | DOI: 10.14710/jkt.v22i1.4304

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The antioxidant activity and total phenolic content of 18 extracts from different plant parts (leaves and stem bark) of 3 mangroves R. mucronata,  R. apiculata andR. stylosa from Teluk Awur, Jeparawere examined. The content of total phenolics in the extracts was calculated as gallic acid equivalent (GAE) and antioxidant activity was estimated as IC50 values using 1,1-diphenyl-2-picrylhydrazyl (DPPH). The samples were extracted with n-hexane, ethyl acetate, and methanol respectively. The result showed that ethyl acetate extract of stem bark of R. apiculata holded the highest antioxidant activity with IC50 47.52ppm. Rates of total phenolic content ranged from 5.15 ± 1.02 to 64.79 ± 2.75 mg GAE/g.There was a significant correlation between total phenolic content and antioxidant activity. The results indicated promising mangrove Rhizophora sp. for the utilization as significant source of natural antioxidant. Telah dilakukan uji kandungan total fenolat dan aktivitas antioksidan 18 ekstrak dari bagian tanaman (daun dan kulit batang) 3 spesies mangrove asal Teluk Awur Jepara, yaitu mangroves R. mucronata,  R. apiculatadan R. stylosa. Kandungan total fenolat ditentukan sebagai ekuivalen asam galat (GAE) dan aktivitas antioksidan ditentukan berdasarInhibition Concentration 50 (IC50) dengan menggunakan radikal bebas DPPH (1,1-diphenyl-2-picrylhydrazyl). Sampel diekstraksi secara bertngkat menggunakan n-heksana, etil asetat dan metanol. Hasil penelitian menunjukkan ekstrak etil asetat kulit kayu mangrove R. apiculata memiliki aktivitas antioksidan terbaik dengan IC5047.52ppm. Nilai kandungan total fenolat ekstrak berkisar antara 5.15 ± 1.02 sampai  64.79 ± 2.75 mg GAE/g. Terdapat korelasi kuat antara kandungan total fenolat dengan aktivitas antioksidan. Hasil ini menunjukkan bahwa mangrove Rhizophora sp. dapat dijadikan sebagai sumber senyawa antioksidan alami.
Profil Vertikal Logam Berat Tembaga (Cu), Nikel (Ni), dan Mangan (Mn) di Core Sedimen Perairan Pantai Marunda, Teluk Jakarta Kresna Rangga Darmansyah; Sri Yulina Wulandari; Jarot Marwoto; Endang Supriyantini
Jurnal Kelautan Tropis Vol 23, No 1 (2020): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v23i1.5667

Abstract

Heavy metals contained in the waste  enter to the Marunda coastal waters  in Jakarta Bay through the Tiram river, come from the Jabodetabek region which is an industrial and residential area.  They  will be deposited and accumulated in the bed sediments and potentially to cause contamination in the waters as well. The study was conducted to investigate vertical profiles  of heavy metals Cu, Ni, and Mn based on sediment depth and the correlation between the concentration of heavy metals and the grain size of sediments. Coring method was used for sampling the sediments at 1-3 cm, 4-6 cm and 7-9 cm of depths.  Samples were analyzed using AAS method to determine Cu, Ni, and Mn concentrations. Sieving and pipetting methods were used to determine the grain size of the sediments. The results showed that the average concentration of heavy metals Cu, Ni, and Mn in the top layer  was 7.74 ppm; 8.17 ppm; 1345.03 ppm. The middle layer was 6.91 ppm; 7.48 ppm; 1279.35 ppm. The bottom layer was 7.36 ppm; 7.79 ppm; 1413.66 ppm. The concentration of Cu heavy metal had a positive correlation with the sand fraction, while the heavy metals Ni and Mn  had a positive correlation with the mud fraction. Logam berat yang terkandung dalam limbah masuk ke perairan pantai Marunda di Teluk Jakarta melalui sungai Tiram, berasal dari daerah Jabodetabek yang merupakan kawasan industri dan pemukiman. Logam berat tersebut akan  mengendap dan terakumulasi di sedimen dasar perairan dan berpotensi menimbulkan pencemaran perairan. Penelitian ini bertujuan untuk mengetahui profil logam berat Tembaga, Nikel, dan Mangan secara vertikal berdasarkan kedalaman sedimen dan korelasi antara konsentrasi logam berat dengan ukuran butir sedimen. Pengambilan sampel sedimen dilakukan menggunakan metode  coring untuk kedalaman 1-3 cm, 4-6 cm, dan 7-9 cm. Selanjutnya sampel sedimen dianalisis menggunakan metode AAS untuk mengetahui konsentrasi logam berat Cu, Ni, dan Mn. Metode sieving dan pipetting digunakan untuk mengetahui ukuran butir sedimen. Nilai konsentrasi rata-rata logam berat Cu, Ni, dan Mn  pada lapisan atas untuk setiap stasiun berurutan sebesar 7,74 ppm; 8,17 ppm; 1345,03 ppm. Lapisan tengah sebesar 6,91 ppm; 7,48 ppm; 1279,35 ppm. Lapisan bawah sebesar 7,36 ppm; 7,79 ppm; 1413,66 ppm. Konsentrasi logam berat Cu mempunyai korelasi positif terhadap fraksi pasir, sedangkan logam berat Ni  dan Mn mempunyai korelasi positif terhadap fraksi lumpur.
Co-Authors Ade Kurniawan Ade Kurniawan Adi Santosa Agus Dermawan Agus Trianto Ahmad Rayyis Ali Djunaedi Ali Djunaedi Ali Ridlo AMANDA, REGINA Ambariyanto , Ambariyanto Ambariyanto Amtoni Caesario Nainggolan Anggit Puji Muswantoro Anggit Puji Muswantoro Anindya Putri Fadmawati Arif Maa’ruf Al Ayyub Bahry, Muhammad Syaifudien Bambang Yulianto Chika Velita Anindya Yulian Chrisna Adhi Suryono Cintya Pramesthi Dewi Dimas Panji Budi Prasetyo Dinda Ayuniar Zanjabila Diyah Putri Ambarwati Dwi Haryo Ismunarti Dwi Nur Hanifah Eka Mulya Eko Wardana Parsaulian Tampubolon Emia Sayniri Sembiring Emilia Sijabat, Emilia Erick Samuel Frederico Hasibuan Ervia Yudiati Ester Tiurlan Faith Dibri Kimberly Fera Nur Idawati Sahara Fitrianisa Nur Widasari Fitrianisa Nur Widasari Gunawan Widi Santosa Hadi Endrawati Ibnu Wardani Ita Widowati Jamaludin Jamaludin Jarot Marwoto Khoirunnisah Riswanti Koesoemadji Koesoemadji Kresna Rangga Darmansyah Ladies Nikita Alamanda Lilik Maslukah Linggar Dirgantara Prasetyo Liningga Adiningtyas Mahadika Fanindhita Sany McCauley, Erin Mimie Saputri Muhammad S. Bahry Murraya Murraya Murraya, Murraya Mutiara Mega Septiningtyas Mutiara Nurul Fajar Utami Nada Kristiani Ginting Nadya Oktavia Nevanda Kusuma Wardani Nirwani Soenardjo Nirwani Soenardjo Nugroho Hendartono Nugroho Hendartono Nur Islamiah Sulastri Nur Taufiq-Spj Nuril Azhar Ocky Karna Radjasa Oetari Kusuma Putri Pramastuti, Fransisca Ria Raden Ario Rayyis, Ahmad Regina Amanda Ria Azizah Tri Nuraini Ria Azizah Trinuraini, Ria Azizah Rima Rosema Rini Pramesti Rizky Budhi Kusuma Rizky Rifatma Jezzi Rizqi Umi Arifah Rudhi Pribadi Sabrina Arifiani Nurtania Sri Redjeki Sri Sedjati Sri Yulina Wulandari Sunaryo Sunaryo Suryono Suryono Susilo Dwi Cahyanti, Susilo Dwi Tampubolon, Eko Wardana Parsaulian Taufiq-Spj, Nur Titik Mariyati Tunas Pulung Pramudya Victorina Yulina Santi Vivian Ayu Cyntya Wardani, Ibnu Widianingsih Widianingsih Wismayanti, Gita Yanuar Sandy Perdana Yanuar Sandy Perdana Yovita Noor Hidayah Yusup Bayu Permadi Yusup Bayu Permadi Zidny Nurfadhli