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All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Journal of Marine Research Jurnal Kelautan Tropis Panrita Abdi - Jurnal Pengabdian pada Masyarakat Best Journal (Biology Education, Sains and Technology) Journal of Tropical Pharmacy and Chemistry
Diah Permata Wijayanti
Department Of Marine Science, Faculty Of Fisheries And Marine Science, Diponegoro University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Other

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Fitness of Cassiopea polyps Inoculated with Different Types of Symbionts Mu'alimah Hudatwi; Diah permata Wijayanti; Ambariyanto Ambariyanto; Michio Hidaka
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 27, No 2 (2022): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.27.2.151-158

Abstract

The specificity of the relationship between cnidarian hosts and symbiotic dinoflagellates (zooxanthellae) differs among host species. Some cnidarian hosts can establish symbiotic relationship with various types of zooxanthellae, while others exhibit high fidelity to specific symbiont type. It is not known how compatibility or specificity of the relationship is determined. We hypothesized that some cnidarian hosts select symbiont type that leads to highest fitness when the host is flexible with symbiont type and more than one types of symbionts are available. As a first step to study this possibility, compatibility of clonal polyps of Cassiopea sp. with six strains of cultured zooxanthellae and the fitness of the host associated with different types of symbionts were studied. Polyp diameter was measured and the number of asexual buds were calculated as a measure of host fitness. The number of zooxanthellae in host and in asexual buds was also measured as a measure of symbiont fitness. Three strains KB8 (clade A), Y106 (clade A), and K100 (clade B) were compatible with the Cassiopea polyps, while other three strains, Y103 (clade C), K111 (clade D), and K102 (clade F) were incompatible. No clear difference in the fitness was found among the polyps inoculated with compatible and incompatible symbiont strains. In one experiment, a compatible strain Y106 seemed to decrease host fitness, but this should be checked by further studies. This study suggests that feeding regimes and long observation period might be important when fitness of hosts associated with different types of symbionts is investigated.
PEMBERDAYAAN MASYARAKAT DESA NYAMUK, KECAMATAN KARIMUNJAWA MELALUI TEKNOLOGI PEMBUATAN GARAM MENGGUNAKAN BAKTERI HALOFILIK DAN TEKNIK ULIR FILTER (TUF) Wilis Ari Setyati; Diah Permata Wijayanti; Dwi Haryanti; Andri Cahyo Kumoro
Panrita Abdi - Jurnal Pengabdian pada Masyarakat Vol. 6 No. 3 (2022): Jurnal Panrita Abdi - Juli 2022
Publisher : LP2M Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/pa.v6i3.14622

Abstract

Nyamuk Island is located in the west part of the Karimunjawa islands where most of the local people are fishers who depend on fisheries as their main livelihood. Due to limited knowledge and facilities, many fisheries commodities are not utilized optimally. One of the basic needs of the local community is salt. Salt is an essential food ingredient in life. Community service activities through a creative business development program on salt production are offered as a solution to overcome the problem of the lack of salt quality.  Ideally, salt would be produced using Thread Filter Technology (TUF) with the addition of halophilic bacteria to speed the crystallization process. The quality of seawater influences the success of making salt using halophilic bacteria. Old seawater (with a salt content of at least 20° Be) is an ideal material for manufacturing salt using halophilic bacteria. One method for obtaining old water is salt production through the Thread Filter Technology (TUF) method. The project results are the salt demplots that produce high-quality salt and the availability of alternative livelihoods for the locals in Nyamuk Village. In the long term, alternative livelihoods such as salt making and fisheries product diversification will reduce pressure on the environment and prevent fishing from being carried out extensively. The availability of good quality salt for fish processing can extend fish marketing distribution chain quality and create new job opportunities for homemakers/widows so that they are provided an opportunity to have an income. The project results concluded that the Community Leading Commodity Strengthening activity carried out in the Nyamuk Village was beneficial for the community's welfare and the environment. --- Pulau Nyamuk adalah salah satu pulau di kepulauan Karimunjawa dengan sebagian besar masyarakat menjadi nelayan dan memanfaatkan laut sebagai mata pencaharian utama. Namun karena keterbatasan pengetahuan dan fasilitas banyak hasil tangkap tidak dimanfaatkan secara optimal. Salah satu kebutuhan pokok makanan masyarakat setempat adalah garam. Garam merupakan bahan pokok makanan penting dalam kehidupan, untuk itu perlu upaya pemberdayaan masyarakat tentang pembuatan garam. Kegiatan pengabdian masyarakat melalui program pengembangan usaha kreatif berbasis pembuatan garam ditawarkan sebagai solusi untuk mengatasi permasalahan ketiadaan garam berkualitas di Desa Nyamuk. Pembuatan  garam idealnya dilakukan dengan menggunakan penambahan bakteri halofilik yang bertujuan mempercepat proses kristalisasi garam. Keberhasilan pembuatan garam menggunakan bakteri halofilik dipengaruhi oleh kualitas air laut. Air laut tua (yang memiliki kadar garam minimal sama dengan 20° Be) merupakan bahan yang ideal untuk pembuatan garam dengan memanfaatkan bakteri halofilik. Salah satu metode untuk memperoleh air tua dalam pembuatan garam adalah melalui metode Teknologi Ulir Filter (TUF).  Hasil pengabdian ini merupakan alternatif tersedianya mata pencaharian bagi penduduk Desa Nyamuk. Hal tersebut merupakan upaya untuk mengurangi tekanan terhadap lingkungan jika terus-menerus dilakukan penangkapan ikan tanpa jeda buka tutup aktivitas penangkapan. Tersedianya garam berkualitas untuk pengolahan ikan diharapkan dapat memperpanjang kualitas ikan maupun rantai distribusi pemasaran ikan. Disamping itu juga tersedianya lapangan kerja baru bagi para ibu rumah tangga/janda sehingga memiliki peluang untuk memiliki penghasilan. Hasil pengabdian dapat disimpulkan bahwa kegiatan Penguatan Komoditi Unggulan Masyarakat (PKUM) yang dilaksanakan di Desa Nyamuk bermanfaatkan untuk kesejahteraan masyarakat dan lingkungan.       
Isolasi, Identifikasi dan Karakteristik Antimicrobial Resistance Staphylococcus cohnii dari Perairan Semarang Siti Nur Kholishah; Diah Permata Wijayanti; Mada Triandala Sibero
BEST Journal (Biology Education, Sains and Technology) Vol 5, No 1 (2022): Juni 2022
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v5i1.5295

Abstract

Resistensi bakteri terhadap antibiotik merupakan salah satu masalah utama di bidang kesehatan saat ini, salah satunya adalah bakteri Staphylococcus cohnii. Bakteri tersebut dapat hidup di berbagai habitat termasuk lingkungan perairan. Cemaran antibiotik di lingkungan perairan adalah salah satu faktor yang meningkatkan resistensi bakteri. Penelitian ini bertujuan untuk mengetahui profil resistensi bakteri Staphylococcus cohnii dari perairan Semarang. Isolat didapat dari koleksi Laboratorium Natural Product Universitas Diponegoro. Isolat kemudian dikarakterisasi secara morfologi dan diidentifikasi secara molekuler. Selanjutnya resistensi isolat terhadap antibiotik diuji dengan metode Antimicrobial Susceptibility Test (AST). Diameter zona bening kemudian diukur setelah 24 jam dan disesuaikan dengan standar CLSI 2021. Karakteristik morfologi koloni ATL.215 yaitu berbentuk cicular atau bulat dengan elevasi convex atau cembung di bagian tengah dan margin entire atau tepian yang halus. Warna isolat pada kedua media adalah putih. Isolat diidentifikasi sebagai Staphylococcus cohnii berdasarkan identifikasi molekuler sekuens 16s rRNA dengan percent identity 99,93% dan query cover 100%. Berdasarkan hasil AST, isolat menunjukkan resistensi terhadap satu dari delapan antibiotik yaitu chloramphenicol dengan diameter zona bening 8,55 mm. Sedangkan pada tujuh antibiotik lain diameter zona bening yang didapatkan termasuk dalam kriteria susceptible.
Tingkah Laku dan Kemunculan Hiu Paus (Rhincodon typus, Smith 1828) di Pantai Bentar Probolinggo Puspa Kapinangasih; Diah Permata Wijayanti; Agus Sabdono
Journal of Marine Research Vol 11, No 1 (2022): Journal of Marine Research
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v11i1.31727

Abstract

Hiu Paus diketahui muncul di Pantai Bentar dari bulan Desember hingga Maret. Kemunculan Hiu Paus  diduga berhubungn dengan aktivitas makan. Fenomena ini dimanfaatkan oleh masyarakat sekitar Pantai Bentar untuk menarik kedatangan wisatawan. Kondisi tersebut berpotensi menimbulkan dampak negatif bagi Hiu Paus. Penelitian ini bertujuan untuk mengetahui lokasi kemunculan Hiu Paus di Pantai Bentar serta perubahan tingkah laku Hiu Paus saat kegiatan wisata berlangsung. Penelitian dilakukan pada bulan Maret 2021 menggunakan metode observasi langsung dengan melakukan pengamatan di lokasi yang diduga menjadi tempat kemunculan Hiu Paus. Data tambahan diperoleh dari hasil wawancara dengan pengelola dan pemandu wisata. Lokasi kemunculan dicatat menggunakan GPS. Total lokasi kemunculan yang ditemukan berjumlah 9 lokasi yang tersebar di Perairan Gending, yaitu di bagian barat dan timur Pantai Bentar. Kemunculan terbanyak berada di timur Pantai Bentar. Hal ini karena wilayah tersebut dekat dengan bagan dan menjadi wilayah pelayaran nelayan Ikan Teri yang merupakan makanan Hiu Paus. Hiu Paus yang ditemukan berjumlah 9 ekor. Tingkah laku Hiu Paus yang ditemukan ketika aktivitas wisata berlangsung, yaitu perubahan secara perlahan seperti menyelam dan mendekat secara perlahan, perubahan mendadak seperti menjauh secara mendadak, perubahan secara tajam seperti menjauh secara tajam dan tidak merespon. Faktor yang mempengaruhi perubahan tingkah laku diduga karena adanya aktivitas yang dilakukan oleh manusia seperti berenang, mengejar Hiu Paus, melihat dari kapal, pelayaran kapal dan pemberian pakan. Hasil dari penelitian ini diharapkan dapat memberi masukan dalam pengelolaan wisata Hiu Paus Pantai Bentar. Whale sharks are known to appear at Bentar Beach from December to March. The appearance of whale sharks is thought to be related to feeding activities. This phenomenon is used by the community around Bentar Beach to attract tourist arrivals. This condition has the potential to have a negative impact on the whale sharks. This study aims to determine the location of the appearance of whale sharks on Bentar Beach and changes in whale shark behavior during tourism activities. The study was conducted in March 2021 using the direct observation method by making observations at the location suspected to be the place where the Whale Shark appeared. Additional data obtained from interviews with managers and tour guides. The location of occurrence is recorded using GPS. The total location of the emergence found was 9 locations scattered in Gending Waters, namely in the west and east of Bentar Beach. Most occurrences are in the east of Bentar Beach. This is because the area is close to Bagan and is a shipping area for Anchovy fishermen which are the food for whale sharks. There were 9 whale sharks observed. Whale Shark behavior found during tourist activities, namely slow changes such as diving and approaching slowly, sudden changes such as moving away suddenly, sharp changes such as moving away sharply and not responding. Factors that influence behavior change are thought to be due to activities carried out by humans such as swimming, chasing whale sharks, watching from ships, ship sailing and feeding. The results of this study are expected to provide input in the management of Whale Shark tourism in Bentar Beach.
Perkembangan Diatom Perifiton Pada Substrat Buatan di Perairan Pulau Panjang, Jepara Syauqina Nashihi Aufar; Agus Sabdono; Diah Permata Wijayanti; Munasik Munasik
Jurnal Kelautan Tropis Vol 26, No 3 (2023): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v26i3.19779

Abstract

Degradation of marine waters in Panjang Island is increasing resulting in high production of benthic periphyton. Procurement of hard substrate as material for artificial reefs is one of the efforts to see the succession pattern of benthic periphyton microscopically. The purpose of this study was to determine the differences in the abundance and composition of diatoms in geopolymer concrete and ordinary concrete substrates. The method used is the field experimental method, immersing different concrete substrates, namely geopolymer concrete (BA) and ordinary concrete (BT) in the sea waters of Panjang Island at a depth of 3 m for 2 months. Periphyton growth on the substrate was observed microscopically using the Scanning Electronic Microscope (SEM) method. Observations were made at immersion time of 1 day (T1), 7 days (T2), 14 days (T3), 28 days (T4), and 56 days (T5). The results showed that there were differences in the development of Periphyton Diatoms between geopolymer (BA) and ordinary concrete (BT) substrates. On both substrates, periphyton growth was found starting from the observation of soaking for 1 day. The density and diversity of periphyton on the BA substrate was higher than that of the BT substrate, with a density of 2535 cell cm-2 and 73 cell cm-2, respectively, and the diversity of H'Shanon Wiener was 4.07 and 0.33. The benefit of this research is to determine the initial succession of biofilm formation on artificial substrates as a building material for artificial reefs for coral reef restoration programs, especially in marginal reef conditions.  Degradasi perairan laut di Pulau Panjang semakin meningkat mengakibatkan produksi perifiton bentik yang tinggi. Pengadaan substrat keras sebagai bahan terumbu buatan menjadi salah satu upaya untuk melihat pola suksesi perifiton bentik secara mikroskopis.  Tujuan dilakukannya penelitian ini untuk mengetahui perbedaan kelimpahan dan komposisi diatom pada substrat beton geopolimer dan beton biasa. Metode yang digunakan yaitu metode eksperimental lapangan, dilakukan perendaman substrat beton yang berbeda yaitu beton geopolymer (BA) dan beton biasa (BT) di perairan laut pulau Panjangdi pada kedalaman 3 m selama 2 bulan. Pertumbuhan perifiton pada substrat diamati secara mikroskopis menggunakan metode Scanning Electronic Microscope (SEM).  Pengamatan dilakukan pada lama perendaman 1 hari (T1), 7hari (T2), 14 hari (T3), 28 hari (T4), dan 56 hari (T5). Hasil penelitian menunjukkan ada perbedaan Perkembangan Diatom Perifiton antara substrat geopolymer (BA) dan beton biasa (BT). Pada ke dua substrat dijumpai pertumbuhan perifiton mulai pengamatan perendaman selama 1 hari. Densitas dan keragaman perifiton pada substrat BA lebih tinggi daripada substrat BT yaitu masing-masing dengan densitas 2535 sel cm-2 dan 73 sel cm-2, dan keragaman H’Shanon Wiener  sebesar 4,07 dan 0,33. Manfaat dari penelitian ini adalah untuk mengetahui suksesi awal pembentukan biofilm pada substrat buatan sebagai bahan penyusun terumbu buatan untuk program restorasi terumbu karang khususnya pada kondisi terumbu marginal.
Bacterial Symbionts of Soft Coral Lobophytum Sp. From Panjang Island, Jepara, Indonesia With Antimicrobial MDR TB Potency Yuvianti Dwi Franyoto; Ahmad Fuad Masduqi; Sakti Muchlisin; Prasetyo Abi Widyananto; Sulistiyani Sulistiyani; Diah Permata Wijayanti; Lia Kusmita
Journal of Tropical Pharmacy and Chemistry Vol. 4 No. 5 (2019): J. Trop. Pharm. Chem.
Publisher : Faculty of Pharmacy, Universitas Mulawarman, Samarinda, Indonesia, 75117, Gedung Administrasi Fakultas Farmasi Jl. Penajam, Kampus UNMUL Gunung Kelua, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jtpc.v4i5.200

Abstract

Tubercolusis is a disease that attacks the lungs. The disease is caused by the bacterium mycobacterium tubercolusis. The bacteria mycobacterium tubercolusis can be killed by antibiotics. However, continuous use of antibiotics can cause bacterial resistance. So we need to find new drugs that can prevent multi-drug resistant tuberculosis. This study aims to determine the antibacterial activity of MDR TB from soft coral symbiont bacteria Lobophytum sp. There were 6 bacterial isolates obtained from soft coral Lobophytum sp. One isolate from Lobophytum-associated bacteria were successfully screened for antimycobacterial against MDR TB bacteria. PLO2 was found to inhibit the growth of MDR TB (MDR TB strain SIRE and R). Based on the results of identification with PCR, soft coral symbionts of PLO2 was closely related to Virgibacillus marismortui with homology of 99%.
Co-Authors Afrinal Pilly Agus Sabdono Agus Trianto Ahmad Fuad Masduqi Aji Wahyu Anggoro Alferd Y. Ko'ou Ambariyanto Ambariyanto Ambariyanto Ambariyanto Analis Finansi Twindiko Andi Afriandi Andri Cahyo Kumoro Andrianus Sembiring Aufa Rifqi Widiardja Ayu Charismawaty Azis Rifai Bambang Yulianto Bayu Kreshna Adhitya Sumarto Bima Fatah Alam Burhan Habibi Yunus Christopher P. Meyer Destio . Dwi Haryanti Dwi Haryo Ismunarti Eka Maya Kurniasih Eko Puji Hartono Elis Indrayanti Ervia Yudiati Franyoto, Yuvianti Dwi Hendro Kisworo Hendry Syahputra Ropinus Siagian Hudanu Hapsara Ibnu Pratikto Irwani Irwani Justin Cullen Lia Kusmita Luh Putu Puspita Dewanti Lutfillah Arief Ghinaa Shabrina Mada Triandala Sibero Mea Pradnawati Michio Hidaka Michio Hidaka Mila Safitri Rizfa Muhammad Dailami, Muhammad Mustagfirin Mustagfirin Mu’alimah Hudatwi Mu’alimah Hudatwi Ni Kadek Dita Cahyani Ni Made Ernawati, Ni Made Ni Putu Dian Pertiwi Nur Taufiq-Spj Prastyo Abi Widyananto Puji Eka Purnama Puspa Kapinangasih Raden Ario Radisya N Nissa Retno Hartati Ria Azizah Tri Nuraini Rivan Novianto Madilana Rudi Pribadi Sakti Muchlisin Siti Nur Kholishah Sri Redjeki Subagiyo Subagiyo Sulistiyani Sulistiyani Suryono Suryono Suzana Kristy Satriani Fofied Syahrial V. Canavaro Syauqina Nashihi Aufar Tiara Anggita Tiwi Ajeng Prameliasari Widy Febriansyah Wilis Ari Setyati Yoko Nozawa