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Journal : Journal of Tropical Crop Science and Technology

The Effect of BAP and Kinetin Concentrations for shoot induction on Teak (Tectona grandis L.) with In Vitro method Prameswari, Mayang Arnindika; Karno, Karno; Anwar, Syaiful
Journal of Tropical Crop Science and Technology Vol. 1 No. 2 (2019): VOLUME 1, NO. 2, OCTOBER 2019
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/jtcst.v1i2.10417

Abstract

The purpose of this research was to examine interaction effect between the different concentration of BAP hormone (Benzylaminopurine) and Kinetin hormone on the shoot induction of Jati (Tectona grandis L.) with in vitro method. This research used a completely randomized factorial design with 4x3 factorial pattern. The first factor was the concentration of BAP (B0 = 0 ppm; B1 = 1 ppm; B2 = 2 ppm dan B3 = 3 ppm). The second factor was concentration of Kinetin (K0 = 0 ppm; K2 = 0,75 ppm; dan K3 = 1,5 ppm). The parameters observed were time of callus appearance, callus length, time of shoot emergance, number of shoots, length of shoots, time of leaf development, number of leaves, percentage of growth, and percentage of contamination. The parameters obtained were analysed descriptively because the percentage of total growth was under 50%. The result showed that the concentration 1 ppm BAP and 0,75 ppm kinetin was better for stimulating the appearance of callus, shoots, leaves, and number of shoots. Callus diameter was better at concentration of BAP 3 ppm. The callus texture that was formed was included in the compact texture. The percentage of explants life was 33,30 – 66,60%. No contamination occurred in all explant samples.
Growth and Production of Chinese Kale (Brassica oleracea L.) on Different Growth Media and AB Mix Substitution with Organic Nutrition of Float Hydroponic Systems Sinaga, Corry Dominika; Karno, Karno; Purbajanti, Endang
Journal of Tropical Crop Science and Technology Vol. 2 No. 2 (2020): VOLUME 2, NO. 2, OCTOBER 2020
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/jtcst.v2i2.10549

Abstract

This research aimed to assess the effect of several types of growing media and AB Mix substitution with organic nutrition on Kailan (Brassica oleraceae L.) growth. The study used a factorial 3 x 4 completely randomized design (CRD) with 3 replications. The first factor is the treatment of growing media which consists of Rockwool, Charcoal husk, and Cocopeat. The second is the AB Mix Substitution with organic nutrition consisting of AB Mix substitution with 0% organic nutrition, AB Mix Substitution with 25% organic nutrition, AB Mix Substitution with 50% organic nutrition, and AB Mix Substitution with 100% organic nutrition. Growth parameters observed included plant height, number of leaves, root length, canopy wet weight, root wet weight, canopy dry weight, root dry weight, and chlorophyll. Data were analyzed using ANOVA and continued by DMRT (Duncan's Multiple Range Test) at 5% level. The results showed that the planting media treatment increased growth in the plant height, number of leaves, root length, canopy wet weight, root wet weight, and canopy dry weight. The treatment of AB Mix substitution with 25% organic nutrition is equivalent to the treatment with 0% organic nutrition for all parameters except chlorophyll. The AB Mix substitution treatment with 50% organic nutrition is not equivalent to the treatment without substitution. The higher substitution treatment will affect growth.
Effect of KNO3 Concentration and Fruit Maturity Levelon the Viability of Papaya Seed (Carica papayaL.) cv.Callina pamungkas, wahyu widi kusuma; Kusmiyati, Florentina; Karno, Karno
Journal of Tropical Crop Science and Technology Vol. 2 No. 1 (2020): VOLUME 2, NO. 1, MARET 2020
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/jtcst.v2i1.10624

Abstract

The research was aimed to examine the effect of different concentration of KNO3 and fruit maturity level on the seed viability of papaya. This research used Completely Randomized Design (CRD) factorial with two main treatments. The first treatment was fruit maturity level (A0: 75% dan A1: 100%). The second treatment was concentration of KNO3 (B1 = 0 M ; B2 = 0,05 M ; B3 = 0,1 M ; B4 = 0,15 M ; B5 = 0,2 M). Seedling parameters observed were germination rate, seed growth rate, vigour index, and maximum growth potential. Nursery parameters observed were plant height and leaf area. Data were analyzed with Analysis of Variance (ANOVA) followed by Duncan Multiple Range Test (DMRT) at 5%. The interaction between fruit maturity and KNO3 concentration showed significantly in germination rate, seed growth rate, vigour index, maximum growth potential and plant height. Leaf area were onlyaffected by KNO3 concentration. This showed that fruit maturity level and KNO3 concentration were significantly affects seed viability.