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Ferry Fredy Karwur
Universitas Kristen Satya Wacana
Agriculture, Biological Sciences & Forestry Arts Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Neuroscience Nursing Physics Public Health Transportation Other

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Journal : Microbiology Indonesia

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Exploration, Isolation and Quantification of β-carotene from Bacterial Symbion of Acropora sp. NAELY K. WUSQY; LEENAWATY LIMANTARA; FERRY F KARWUR
Microbiology Indonesia Vol. 8 No. 2 (2014): June 2014
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (257.52 KB) | DOI: 10.5454/mi.8.2.3

Abstract

In the microbial world, pigments are one of the most conspicuous traits. Marine bacteria associated with Acropora sp. collected from Taka Cemara, Karimunjawa Islands were screened for the production of a yellow pigment. The isolation of bacterial symbionts from Acropora sp. on Zobell 2216E medium resulted in one bacterium, KJ5, positively synthesized carotenoids. By reverse phase HPLC analysis, one peak of the pigment types was identified as a β-carotene peak which appeared at 60.24 min. Then, sample of the β-carotene was collected and identified according to their spectral characteristics and compared with the published data in different types of solvent. Based on the HPLC analysis, the total β-carotene contents were calculated by converting the broad absorption of β-carotene. Molecular identification of the bacterium KJ5 using 16S rDNA showed that bacterium KJ5 was closely related to Erythrobacter flavus with 96% homology value.
Co-Authors A. B. Susanto Albina Eva Yolanda Anggreni Beatris Liunokas Angkit Kinasih Angreni B Liunokas Antonius Budi Susanto Binerd Anthon Im Toy Dalengkade, Mario Nikolaus Desi Desi Dispanstiani Abidin Dwi I Prayitmo Ferdy S Rondonuwu Ferdy S. Rondonuwu Ferdy S. Rondonuwu Ferdy Semuel Rondonuwu Fiktor I. Boleu Fitria Fitria Frity Lisa Taroreh Frity Lisa Taroreh Handung Nuryadi Haryono Semangun Iqna Kamila Abfa Jerry F Langkun Jubhar C Mangimbulude Jubhar C. Mangimbulude Jubhar C. Mangimbulude Jubhar Mangimbulude Junet F. da Costa Junet. F. da Costa Kristiani Desimina Tauho Kukuh Pambuka Putra Leenawaty Limantara Leviano Leviano Lodu, Widiyasri M. Rio Syahputra Martanto Martosupono Martanto Martosupono Mayang Januarti Permatasari Monika Rahardjo Naely K Wusqy NAELY K. WUSQY Naely Kurnia Wusqy Nur Auliani Lestariningrum, Nur Auliani Nurul Wahidatul Hidayati Ocky K Radjasa Ocky Karna Radjasa Prameshwari, Deshinta R.I.N.K Retno Triandhini Rambu L.N.K. Retno Triandhini Renny Indrawati, Renny Retno Triandhini Ria Novianti Ronaldo Lomi Djara Rosiana Eva Rayanti Rully Toar Tumanduk Sabrina Aprilisa Martha Sonya Titin Nge Venti Agustina Widiyasri Lodu Windu Merdekawati Wiwik Astuti Wiwik Astuti