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All Journal ACTA VETERINARIA INDONESIANA Indonesian Journal of Electronics and Instrumentation Systems METAMORFOSA Journal of Biological Sciences Jurnal Ilmu Dasar AL KAUNIYAH BERKALA SAINSTEK Biogenesis: Jurnal Ilmiah Biologi UNEJ e-Proceeding Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Universa Medicina Jurnal Bioteknologi & Biosains Indonesia (JBBI) BIOEDUKASI Warta Pengabdian Jurnal Biolokus: Jurnal Penelitian Pendidikan Biologi dan Biologi Bioma : Jurnal Biologi dan Pembelajaran Biologi Jurnal Riset Biologi dan Aplikasinya Life Science and Biotechnology Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Rike Oktarianti
Jurusan Biologi FMIPA Universitas Jember, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Control & Systems Engineering Education Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Veterinary

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Journal : Jurnal Ilmu Dasar

 1 
Studies Polymorphism of Blood Plasm Protein in Gurami (Osphronemus gouramy Lac) in Jember County Rike Oktarianti; Mamik Pristiwindari
Jurnal ILMU DASAR Vol 8 No 1 (2007)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (172.51 KB)

Abstract

The objective of this research was to study the blood protein polymorphism of gurami (Osphronemus gouramy Lac). Blood sample were collected from 55 gurami. Polyaacrilamide Gel Electrophoresis (PAGE) was used in the blood plasm protein analysis. Identification of protein bands on gel are determinated by comparing the molecul weight of those test group protein with the marker protein. The result showed that the locus albumin, pre-albumin and transferin are polymorphic. Locus albumin, pre-albumin and transferin are controlled by three alleles ie A, B and C. The total heterozygozity of gurami is 52 %.
Identification of Immmunogenic Salivary Proteins of Anopheles vagus based on Mass Spectrometry Analysis Febriyantiningsih, Dwi Esti; Senjarini, Kartika; Oktarianti, Rike
Jurnal ILMU DASAR Vol 18 No 2 (2017)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (702.853 KB) | DOI: 10.19184/jid.v18i2.3106

Abstract

Malaria has been prevalent for a long time in tropical developing regions causing great morbidity and mortality. Among the malaria vectors, Anopheles vagus has been known as secondary malaria vector in East Java. Salivary glands of mosquitoes perform various functions for survival of the vectors and also conducive for blood feeding, harbouring of malaria parasites, and eventual parasite transmission. The salivary gland proteomes of An. vagus have not been carried out yet. The aim of our study was to identify and characterize the immunogenic proteins of salivary glands proteins of An. vagus. A proteomic approach combining one-dimensional electrophoresis (1DE) followed by western blot analysis using human sera from healthy people living in an endemic area (Kendal); liquid chromatography mass spectrometry (LC-MS/MS) and bioinformatic analysis was adopted to provide the first direct insight into identification and characterization of salivary proteins of An. vagus. Identification of immunogenic proteins using western blot analysis has revealed three immunogenic bands which had molecular weights of 69, 75 and 232 kDa. Among those proteins analysed by LC-MS/MS, there were alpha,1-4 glucan phosphorylase, putative myosin class I heavy chain which have the highest number of total spectrum count peptide. Other proteins like vitellogenin and heat shock protein 82 (Hsp82) were also identified. The majority of proteins were scrutinized marked for their role in metabolism, cytoskeleton protein and stress response. Keywords: Anopheles vagus, salivary gland, immunogenic, proteomics
The Diversity of Potential Malaria and Dengue Mosquito Vector from Bangsring Village Wongsorejo District Banyuwangi East Java Azkiyah, Siti Fat'hiyatul; Senjarini, Kartika; Oktarianti, Rike; Wiyono, Hidayat Teguh; Wathon, Syubanul
Jurnal ILMU DASAR Vol 22 No 1 (2021)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v22i1.13601

Abstract

Bangsring village in Wongsorejo regency has been reported as malaria endemic area in Banyuwangi since 2011. Understanding the diversity and behavior of mosquito vector in this area will be very impotant in developing vector control program. The masquitoes were collected by landing collection outside and inside house by human bites, we also observed the area around cattle cage. During observation periods, a total 633 masquitos were collected consist of 44% malaria potential vector (Anopheles spp.) 19% Dengue fever (DF) potential vector (Aedes spp.) and 36% were not belong to both of them. Out of total collected Anopheles spp. mosquitoes about 65% were indentified as An. vagus, 25% were An. indevinitus, 8% were An. vagus (limosus), and only 2% as well as 0,04 % were An. supictus and An. kochi. Meanwhile we found 19% of Aedes sp. were Ae. aegypti and 81% were Ae. albopictus. This study showed that the dominan potential malaria’s vector is An. Vagus and the dominan DF vector is Ae. albopictus. Mostly colleted Anopheles sp. were exophagic and zoophilic with the highest activities between 06.00-08.00 pm. This was in contras to Aedes sp. where mosfly found endophilic and it has 2 hightime of activities Aedes sp. between 06.00- 07.00 AM and 05.00-06.00 PM. Keywords: Dengue, Malaria, diversity, Anopheles, Aedes.
Co-Authors Agustin, Dita Paramytha Ahmad Tosin Aida Mursyidah Ainiyah, Durotun Amania, Novita Aminuyati Anam, Khairul Ardyah, Naura Paramitha Cindy Ari Satia Nugraha Arofa, Resmining Mega Azkiyah, Siti Fat'hiyatul Berlian Permata Dewi Erlambang Cizein Tri Cahyanti Devi Astikaningrum Devi Astikaningrum Dewi Masruroh Dita Paramytha Agustin Eva Tyas Utami Febriyantiningsih, Dwi Esti Fitria Mutiah Fitria Muti’ah Hidayat Teguh Wiyono Husnatun Nihayah Iflaha, Adriana Ihsanul Azmi Indriani, Fenny Intan Fitri Indrasari Kartika Senjarini Khasanah, Rochmatul Nuryu Kirana Eka Rezki Labes, Antje Laili, Alvina Nur Lailly Nur Uswatul Hasanah Lelono, Asmoro Lutfia Azizah Mahriani Mahriani Mamik Pristiwindari MAsruroh, Binti Maulana Jauharil Habib Miatin Alvin Septianasari Mochtar Gunawan Wibisono Muhammad Khalid Abdullah Mutiah, Fitria Nadya Rismana Fitriani Nailatur Rifdah Naura Paramitha Cindy Ardyah Naura Paramitha Cindy Ardyah Novita Amania Nur Aisyah Septiana Nuril Azizah Purwatiningsih Purwatiningsih Putra, Iguh Widigda Ratis Nour Sholichah Rehmann, Holger Renam Putra Arifianto Rendy Setiawan Riana Agatha Listiani Saraswati, Dyah Rahmadhani Silvya Fitri Nur Azizah Siti Agustina Wulandari Sri Mumpuni Sri Mumpuni Wahyu Widajati, Sri Mumpuni Wahyu Syubbanul Wathon, Syubbanul Syubbanul Waton Syubhanul Wathon Tri Yudani MR Utami, Diah Ayu Wahyu Tri Agustin Wathon, Syubanul Wathon, Syubbanul - Widya Cahyadi Widyadana, Kaysha Rahmadita Wulandari, Ayu Dwi Yasir Mubarok Yenny Febriana Ramadhan Abdi Zahniar, Zahniar