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All Journal Buletin Peternakan Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Matematika Sains dan Teknologi Agrinimal Jurnal Ilmu Ternak dan Tanaman INDRA: Jurnal Pengabdian kepada Masyarakat Pakem : Jurnal Pengabdian Kepada Masyarakat Jurnal Teknologi Lingkungan Lahan Basah Jurnal Kedokteran Hewan Jurnal Nusantara Mengabdi
Rony Marsyal Kunda
Department Of Biology, Faculty Of Mathematics And Natural Science, Universitas Pattimura, Ambon, 97233, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Preliminary PCR Screening and Optimization for Five Suspected Viruses in Haemaphysalis bispinosa Ticks from Moa Buffalo (Bubalus bubalis) Prasetyarti Utami; Rony Marsyal Kunda
Jurnal Penelitian Pendidikan IPA Vol 12 No 1 (2026)
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v12i1.13910

Abstract

Tick-borne viruses are becoming a serious threat to livestock health, especially in tropical regions where monitoring systems are still limited. H. bispinosa, the main external parasite of water buffalo in Eastern Indonesia, is suspected of carrying several veterinarians' important viral pathogens. This study aims to detect five viruses consisting of DNA viruses (BuGyKV, BoHV-1, and OvHV-2) and RNA viruses (FMDV and TAMV). FMDV and TAMV, both RNA viruses, need to be converted into complementary DNA (cDNA) through reverse transcription, which is then used as a template for PCR amplification using virus-specific primers. All viruses were detected using specific primers and extensive annealing temperature optimization. The results showed weak and nonspecific amplification bands at one temperature for BuGyKV, BoHV-1, and OvHV-2, but these bands did not match the expected amplicon size. No amplification bands were observed for FMDV and TAMV under any conditions. These findings suggest that this amplification failure is likely due to primer mismatch with local virus variants resulting from geographical differences. Overall, it can be concluded that the five target viruses were not detected in the H. bispinosa samples examined. This preliminary study provides an initial virological overview of H. bispinosa ticks from Moa Island and emphasizes the need for more sensitive detection methods, such as nested PCR, qPCR, or metagenomic approaches, to improve the detection of low-titer viruses in ticks.
Co-Authors Adriana Hakapaa Fredrik Manuhutu Fredrik Manuhutu, Fredrik Habibi Habibi Hendrina Lelloltery Hery Wijayanto Heryanus Jesajas Ivan Mambaul Munir JAMALUDIN JAMALUDIN Jamhari Jamhari Jilian Risky Huwae Lakoan, Milda Rianty Leonard R. Hutasoit Maman Rumanta Maman Rumanta Maria Nindatu Marleny Leasa Marthina Tjoa Maya MS Puttileihalat Mery Pattipeilohy Milda Rianty Lakoan Moniharapon, Mechiavel Niken Satuti Nur Handayani Niken Satuti Nur Handayani Nova, Lucky Starada Pieter Kakisina Porumau, Yandri Yanes Prasetyarti Utami Prasetyarti Utami Prasetyarti Utami Richard R Lokollo, Richard R Rini Widayanti Salamor, Reinhard Sandra Sukmaning Aji Selanno, Frenly M. Slamet Diah Volkandari Slamet Diah Volkandari Sri Kurniati Handayani Ucu Rahayu Utami, Prasetyarti Ventia Cika Tanikwele Woro Danur Wendo Yofian Anaktototy Yuli Tirtariandi El Anshori