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All Journal Buletin Peternakan Jurnal Veteriner Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Matematika Sains dan Teknologi Agrinimal Jurnal Ilmu Ternak dan Tanaman INDRA: Jurnal Pengabdian kepada Masyarakat Pakem : Jurnal Pengabdian Kepada Masyarakat Jurnal Teknologi Lingkungan Lahan Basah Jurnal Kedokteran Hewan Jurnal Nusantara Mengabdi
Rony Marsyal Kunda
Department Of Biology, Faculty Of Mathematics And Natural Science, Universitas Pattimura, Ambon, 97233, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Preliminary PCR Screening and Optimization for Five Suspected Viruses in Haemaphysalis bispinosa Ticks from Moa Buffalo (Bubalus bubalis) Utami, Prasetyarti; Kunda, Rony Marsyal
Jurnal Penelitian Pendidikan IPA Vol 12 No 1 (2026)
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v12i1.13910

Abstract

Tick-borne viruses are becoming a serious threat to livestock health, especially in tropical regions where monitoring systems are still limited. H. bispinosa, the main external parasite of water buffalo in Eastern Indonesia, is suspected of carrying several veterinarians' important viral pathogens. This study aims to detect five viruses consisting of DNA viruses (BuGyKV, BoHV-1, and OvHV-2) and RNA viruses (FMDV and TAMV). FMDV and TAMV, both RNA viruses, need to be converted into complementary DNA (cDNA) through reverse transcription, which is then used as a template for PCR amplification using virus-specific primers. All viruses were detected using specific primers and extensive annealing temperature optimization. The results showed weak and nonspecific amplification bands at one temperature for BuGyKV, BoHV-1, and OvHV-2, but these bands did not match the expected amplicon size. No amplification bands were observed for FMDV and TAMV under any conditions. These findings suggest that this amplification failure is likely due to primer mismatch with local virus variants resulting from geographical differences. Overall, it can be concluded that the five target viruses were not detected in the H. bispinosa samples examined. This preliminary study provides an initial virological overview of H. bispinosa ticks from Moa Island and emphasizes the need for more sensitive detection methods, such as nested PCR, qPCR, or metagenomic approaches, to improve the detection of low-titer viruses in ticks.
Strategi Konservasi Lingkungan Berbasis Kearifan Lokal: Studi Kasus Desa Kersik Tuo, Kerinci Nova, Lucky Starada; Rumanta, Maman; Kunda, Rony Marsyal
Jurnal Nusantara Mengabdi Vol 5 No 2 (2026): Februari
Publisher : Penerbit Goodwood

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35912/jnm.v5i2.6402

Abstract

Purpose: This study examines the role of local wisdom in supporting environmental conservation in Kersik Tuo Village, Kayu Aro District, Kerinci Regency, Jambi Province. Research Methodology: A mixed-methods approach was employed, combining qualitative data collection through interviews and participatory observation with quantitative analysis using SWOT and the Analytical Hierarchy Process (AHP). Results: Local wisdom practices, including hunting prohibitions, crop rotation, and communal land management (gotong royong), remain actively practiced in Kersik Tuo Village. However, these practices face pressures from modernization, environmental degradation caused by intensive agriculture, and declining awareness among younger generations. SWOT and AHP analyses identified three priority conservation strategies: strengthening customary institutions, integrating local knowledge with modern science, and enhancing community participation. Conclusions: Local wisdom has significant potential to serve as a foundation for sustainable and adaptive environmental conservation, particularly in responding to contemporary ecological challenges. Limitations: This study is geographically limited to Kersik Tuo Village, which may restrict the generalizability of the findings to other regions with different socio-cultural conditions. Contributions: This study contributes to the development of community-based conservation frameworks by demonstrating the strategic importance of indigenous knowledge systems as a complement to scientific conservation approaches.
Design and Construction of a Real-Time Air Quality Monitoring System Using IoT-Based ESP32 to Strengthen Environmental Policies El Anshori , Yuli Tirtariandi; Kunda, Rony Marsyal; Manuhutu, Fredrik
Jurnal Penelitian Pendidikan IPA Vol 11 No 2 (2025): February
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i2.9820

Abstract

Air quality monitoring is one of the important steps in maintaining public health and the environment. With the development of Internet of Things (IoT) technology, air quality monitoring can be done in real-time and more efficiently. This study aims to environmental policy and design of an IoT-based air quality monitoring system using the ESP32 microcontroller. This system is designed to measure air quality parameters such as CO, NO2, temperature, and humidity using factory-calibrated sensors (DFRobot) connected to the ESP32 microcontroller. Data obtained from the sensors are processed by the ESP32 and sent to a cloud server via Wi-Fi, allowing real-time monitoring via the ThingSpeak platform which can be monitored via mobile devices or the web. The results of the air quality monitoring system design show that devices using electrochemical CO and NO sensors₂and the SHT30 sensor connected to the ESP32 is capable of reading and measuring CO, NOconcentrations., temperature, and humidity with good accuracy with a sample time of ± 20 seconds. In addition, this system can be connected online with the ThingSpeak platform, allowing visualization of measurement data in graphical form in real-time. Thus, the designed system not only functions optimally in detecting air quality parameters, but also supports efficient remote monitoring through Internet of Things (IoT) technology
Co-Authors Adriana Hakapaa El Anshori , Yuli Tirtariandi Fredrik Manuhutu, Fredrik Habibi Habibi Hendrina Lelloltery Hery Wijayanto Heryanus Jesajas Hutasoit, Leonard Raden JAMALUDIN JAMALUDIN Jamhari Jamhari Jilian Risky Huwae Lakoan, Milda Rianty Leonard R. Hutasoit Lokollo, Richard R. Lokollo, Richard Rudolf Maman Rumanta Maria Nindatu Marleny Leasa Marthina Tjoa Maya MS Puttileihalat Mery Pattipeilohy Milda Rianty Lakoan Moniharapon, Mechiavel Munir, Ivan Mambaul Niken Satuti Nur Handayani Niken Satuti Nur Handayani Nova, Lucky Starada Pieter Kakisina Porumau, Yandri Yanes Prasetyarti Utami Prasetyarti Utami Rini Widayanti Salamor, Reinhard Sandra Sukmaning Aji Selanno, Frenly M. Slamet Diah Volkandari Slamet Diah Volkandari, Slamet Diah Sri Kurniati Handayani Ucu Rahayu Utami, Prasetyarti Ventia Cika Tanikwele Woro Danur Wendo Yofian Anaktototy Yuli Tirtariandi El Anshori