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Journal : Jurnal Sain Veteriner

Pengaruh Ukuran Ovarium dan Folikel terhadap Penampilan Reproduksi Pada Sapi PO dan SimPO di Kecamatan Jatinom, Kabupaten Klaten Priyo Jr, Topas Wicaksono; Budiyanto, Agung; Kusumawati, Asmarani
Jurnal Sain Veteriner Vol 38, No 1 (2020): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (423.167 KB) | DOI: 10.22146/jsv.57362

Abstract

The increasing beef cattle population in Indonesia is not significantly with high demand of meat every year. The insignificant increasing in population is caused by fertility decreasing, infectious disease and the reduction amount of forage land. The factor which causes cattle fertility decreasing can’t be separated from the ovarian and follicular diameter which have an effect on high various reproductive appearance. Parameters that used for knowing reproductive appearance of cattle are Service per Conception (S/C) and Calving Interval (CI). The aims of this study was to describe ovary sizes and follicular diameter in cattle, to describe ovary and follicular diameter toward S/C and CI variation. This research uses female cattle, 8 Simmental-PO (SimPO) and 9 Peranakan Ongole breeds (PO), multiparous, are not pregnant, 4-8 years old, Body Condition Score (BCS) 2.5-3.5, peak phase estrus, has no reproductive problems, clear recordings and live in the area of farmer in Jatinom District, Klaten Regency, Central Java Province. Cattle are examined for ovarian size and follicle size using ultrasonography. S/C and CI data were obtained from records of artificial insemination cards (AI). The data obtained were analyzed by t test. The results of this study showed there were no differences in the diameter of ovaries in the size of SimPO and PO cattle (P> 0.05), there were no differences in the diameter of SimPO and PO cattle follicles (> 0.05), there were differences in ovarian and follicular diameter sizes with respect to S/C and CI in cattle with good and bad reproductive performance (P <0.05). The conclusion of the study showed that there was no difference in ovarian and follicular diameter size in SimPO and PO cattle (P> 0.05) but there was an influence on S/C and CI (P <0.05). 
Efek Penambahan Antioksidan Selenium, Kurkumin dan Kombinasinya Terhadap Motilitas, Recovery Rate dan Viabilitas Spermatozoa pada Kriopreservasi Semen Sapi Peranakan Ongole Yuniar, Resa Miftahatu; Kusumawati, Asmarani; Setyawan, Erif Maha Nugraha
Jurnal Sain Veteriner Vol 42, No 3 (2024): Desember
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.85255

Abstract

Salah satu kendala dalam pelaksanaan kriopreservasi semen adalah tingginya kadar reactive oxygen species (ROS) yang dapat menyebabkan reduksi pada motilitas sperma dan viabilitas sperma post thawing. Strategi untuk mengatasi tingginya kadar ROS adalah dengan menambahkan antioksidan pada pengencer selama proses kriopreservasi. Tujuan dari penelitian ini adalah mengetahui pengaruh penambahan antioksidan selenium dan kurkumin dalam menurunkan stress oksidatif pada proses kriopreservasi semen sapi peranakan ongole (PO). Penelitian ini menggunakan semen yang dikoleksi dari sapi PO dengan vagina buatan. Sampel semen yang ditampung diperiksa secara mikroskopis dan makroskopis kemudian dibagi menjadi 4 perlakuan yaitu kontrol, penambahan selenium (sodium selenite) dengan konsentrasi 50 µM, kurkumin 10 µM dan kombinasi antara selenium 50 µM dengan kurkumin 10 µM.  Semen yang telah dicampur dengan pengencer dan antioksidan kemudian dibekukan dalam proses kriopreservasi, selanjutnya parameter yang diamati meliputi pemeriksaan motilitas sperma, recovery rate dan viabilitas sperma. Semua parameter dianalisis statistika menggunakan One Way ANNOVA dan uji DMRT. Berdasarkan penelitian menunjukkan bahwa perlakuan kurkumin 10 µM memiliki nilai motilitas 48.00 ± 4.47%, recovery rate 66.66 ± 5.54% dan viabilitas 67.00 ± 4.30%, sedangkan perlakuan selenium 50 µM memiliki nilai presentase motilitas 46,00 ± 4,18%, recovery rate 63.90 ± 5.59% dan viabilitas 64.00 ± 3.93%. Pemanfaatan kombinasi kurkumin dan selenium tidak memberikan pengaruh yang nyata dibandingkan dengan kontrol. Kesimpulan penelitian ini adalah penambahan antioksidan kurkumin dan selenium bermanfaat dalam mempertahankan kualitas sperma sapi PO, namun perlakuan kombinasi kurang baik dalam mempertahankan kualitas sperma.
Study of Ovary Follicular Profile and Estrogen Concentration of Peranakan Ongole (PO) Cows at Estrous Phase in Mountains and Lowland at Kulon Progo District, Yogyakarta Fani, Rifia Tiara; Budiyanto, Agung; Kusumawati, Asmarani
Jurnal Sain Veteriner Vol 42, No 2 (2024): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.77613

Abstract

Ovarian follicle dinamycs and estrogen concentration interrelated to cow reproduction traits. Some previous studies said that preovulatory follicle directly related to estrogen concentration which induce estrous quality, and increasing pregnancy rate when artificial insemination happened. Another important factor to optimizing reproduction performance is altitude. Here in Indonesia these factors has not been studied further in PO cows. A total of 24 Peranakan Ongole (PO) cows, 12 cows from highlands or mountains of Kalibawang, Kulon Progo, Yogyakarta, Indonesia and 12 cows from lowlands or lowland of Galur District, Kulon Progo, Yogyakarta, Indonesia selected based on criterias; these cows ever had calves, not pregnant, 3-8 years old, have a Body Condition Score (BCS) of 2.5-3.5, in the peak phase of estrus and not experienced reproductive problems. The diameter of the cow ovarian follicles were examined using ultrasound and sera were taken to calculated the estrogen concentration using ELISA, meanwhile the NSPC taken from recording and farmer interview. The result showed that no difference of follicle diameter and estrogen concentration between mountain and lowland (p>0,05), a significant difference found in NSPC between mountain and lowland (p<0,05), and a strong correlation found betweend follicle diameter, estrogen concentration and NSPC (p<0,05), proved that the larger the follicle diameter, the higher the estrogen level and will improve the reproductive trait of PO cows.