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All Journal HAYATI Journal of Biosciences Media Veteriner Jurnal Sain Veteriner Jurnal Veteriner Jurnal Kedokteran Hewan
Tuty Laswardi Yusuf
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology Medicine & Pharmacology Veterinary

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Kualitas Semen Beku Babi dalam Pengencer Komersial yang Disuplementasi dengan Trehalosa (THE QUALITY OF BOAR FROZEN SEMEN IN COMMERCIAL EXTENDER SUPPLEMENTED WITH TREHALOSE) Tuty Laswardi Yusuf; Raden Iis Arifiantini; Reni Ratni Dapawole; Wilmientje Marlene Mesang Nalley
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (105.27 KB) | DOI: 10.19087/jveteriner.2017.18.1.69

Abstract

The aim of the research was to study trehalosa supplementation in Beltsville Thawing Solution (BTS) and MIII extender on the quality of frozen boar semen. Semen was collected from nine boars age 5-6 years using glove hand method. The semen were then evaluated macro and microscopically. Semen having >70% sperm motility and >200x106/mL sperm concentration were diluted in two tubes containing BTS and two tubes containing MIII, then kept at room temperature (20-22oC) for 2 hours (holding time). Following this the semen were centrifugated at 2000 RPM for 10 minutes and the supernatant was discarded, the pellet was re-diluted with BTS, BTS-Trehalose 100 mM (BTS-T), MIII, and MIII-Trehalose 100 mM (MIII-T) with 4% glycerol, packed into 5 mL macrotube, equilibrate at 5oC for 2 hours. After equilibration macrotube were frozen horizontally 4 cm above the liquid nitrogen vapor for 20 minutes and then submerged in liquid nitrogen (-196oC) for futher evaluation. After 24 hours the semen than thawed at 52oC for 45 seconds. The results demonstrated that sperm motility in BTS-T (30.00±4.47%) was higher (P<0.05) then MIII-T (26.67±8.16%), BTS (24.17±9.17%), or MIII (21.67±7.53%). This research conclude that suplementation of 100 mM trehalosein BTS extender improved motility of boar sperm after freezing and thawing. ABSTRAK Penelitian ini bertujuan untuk mempelajari penambahan trehalosa dalam pengencer Beltsville Thawing Solution (BTS) dan MIII terhadap kualitas semen beku babi. Semen dikoleksi dari sembilan ekor babi berumur 5-6 tahun menggunakan teknik glove hand method. Semen dievaluasi secara makro dan mikroskopis. Semen yang memiliki spermatozoa motil di atas 70% dengan konsentrasi di atas 200x106/mL dibagi ke dalam empat tabung, dua tabung pertama diencerkan dengan BTS dan dua tabung lainnya dengan MIII. Semen yang telah diencerkan disimpan pada suhu ruangan (20-22oC) selama dua jam (holding time). Semen kemudian disentrifugasi 2000 rpm selama 10 menit. Supernatan dibuang dan pellet diencerkan kembali. Semen yang disentrifugasi dengan BTS, diencerkan dengan BTS gliserol (BTSg) dan BTS gliserol Trehalosa 100 mM (BTSgT) dan yang disentrifugasi dengan MIII diencerkan dengan MIIIg dan MIIIgT. Gliserol yang digunakan adalah 4%. Semen yang telah diencerkan dikemas dalam macrotube (5 mL), diekuilibrasi pada 5oC selama dua jam. Setelah equilibrasi macrotube dibekukan 4 cm di atas permukaan nitrogen cair selama 20 menit dan disimpan dalam container nitrogen cair (-196oC) untuk pengujian lebih lanjut. Setelah penyimpanan 24 jam, terhadap semen beku dilakukan thawing pada suhu 52oC selama 45 detik. Hasil penelitian menunjukkan motilitas spermatozoa dalam BTSgT (30,00±4,47%) paling tinggi (p<0,05) daripada MIIIgT (26,67±8,16%), BTSg (24,17±9,17%), atau MIIIg (21,67±7,53%). Simpulan dari penelitian ini adalah penambahan 100 mM trehalosa dalam pengencer BTSg dapat mempertahankan motilitas spermatozoa babi lebih baik daripada pengencer lainnya.
Penentuan Waktu Optimal Kawin Berdasarkan Ultrasonografi Ovarium dengan Gejala Klinis Estrus pada Kambing Peranakan Etawa (DETERMINATION OF OPTIMAL MATING TIME BASED ON OVARY ULTRASONOGRAPHY WITH ESTROUS CLINICAL SYMPTOMS IN ETAWA CROSSBREED GOAT) Yudi Eka Satria; Tuty Laswardi Yusuf; Amrozi .
Jurnal Veteriner Vol 17 No 1 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (215.087 KB)

Abstract

The aim of this study was to determine the optimal mating time in Etawa Crossbred Goat. This studyused 10 ewes, 2-3 years old, with normal estrous cycle. Initiation of estrous using Controlled Internal DrugRelease Implants (CIDR) intra vaginal and released after 12 days. The development of follicles in theovaries wasobserved using transrectal Ultrasonography (USG) every six hours until reach for 66 hoursafter CIDR removal from the vagina. Clinical signs of standing heat and vulva signs were also observedwhile under USG observation. The follicles were divided into three groups based on their diameter:small(<3 mm), medium (4-4.9 mm) and large (>5 mm) which were observed during theestrous phase. Ovulationwas predicted when large follicles (dominant follicles) havenot been seen anymore. The resulta showedthat the average follicles number in ovary after 36 hours CIDR removal were 0-1 (0.8±0.4 mm) small, 0-1(0.9±0.6 mm) medium and 1-2 (1.7±0.8 mm) large, respectively. The large follicles (7-8 mm) wereseen after36 hours CIDR removal or 24 hours after standing heat. Forty two hours after CIDR removal, the largefollicles werenot being seen anymore. It means, the ovulation were predicted in 36-42 hours after CIDRremoval or 24-30 hours after standing heat. The relationship between follicular development and clinical vulva signs (redness, swelling, mucus) revealed that the highest vulva signs (+++) wereseen in 36-42 hoursafter CIDR removal. It was concluded that the ovulation time occurred in 36-42 hours after CIDR removal,with the high intensity of vulva changes. Therefore the optimal mating time in PE 24-36 hours of standingheat or 36-42 hours after CIDR removal.
QUALITY OF CHILLED CANINE SEMEN IN TRIS-EGG YOLK EXTENDER SUPPLEMENTED WITH SERICIN Kim Chwin Khye; Tuty Laswardi Yusuf; Faisal Amri Satrio; Ni Wayan Kurniani Karja
Jurnal Kedokteran Hewan Vol 15, No 1 (2021): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (269.519 KB) | DOI: 10.21157/j.ked.hewan.v15i1.17641

Abstract

                                                                 ABSTRACTThe objective of this research was to evaluate the quality of chilled canine semen in Tris-egg yolk (TEY) extenders containing different concentrations of sericin. Semen were collected from four dogs by massage method. Canine semen was collected using sterile urine pots and evaluated. Sperm-rich fractions were pooled and divided into four equal aliquots, which were then diluted with TEY extenders supplemented with different concentrations of sericin (0%, 0.1%, 0.25%, 0.5%). The diluted semen aliquots were preserved at 4 ℃ in sterile centrifuge tubes and were then evaluated for spermatozoa motility, viability, plasma membrane integrity and acrosome integrity every 12 hours up to 72 h. The TEY extenders supplemented with 0.25% and 0.5% sericin resulted in higher spermatozoa motility and viability at 72 h compared to other TEY extenders (P0.05). The integrity of plasma membrane and acrosome of spermatozoa showed no significant differences among the groups extenders at 72 h. In conclusion, sericin in concentration of 0.25% and 0.5% were able to prevent the motility and viability of canine spermatozoa after storage for 72 h.
Co-Authors Amrozi Anak Agung Gede Sugianthara Arief Boediono Bambang Purwantara Dondin Sajuthi ERIC HAYES Faisal Amri Satrio GONO SEMIADI Gozali Moekti HERA MAHESHWARI Iman Supriatna Kim Chwin Khye Lies Parede Hernomoadi LUTHFIRALDA SJAHFIRDI Luthfiralda Sjahfirji M Agus Setiadi MUHAMMAD AGIL Ni Wayan Kurniani Karja Oriza Savitri Ariantie PUDJI ASTUTI R. Iis Arifiantini Rachmat Herman Reni Ratni Dapawole Ristika Handarini Rusdin - Satya Gunawan Solly Aryza Srihadi Agungpriyono Sriyanto - Teguh Budipitojo Wahyuni, Sri Wilmientje Marlene Mesang Nalley Yudi Eka Satria