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All Journal Jurnal Teknologi Dan Industri Pangan Natural Science: Journal of Science and Technology JOURNAL OF COASTAL DEVELOPMENT ILMU KELAUTAN: Indonesian Journal of Marine Sciences Reaktor Jurnal Natur Indonesia Microbiology Indonesia AL KAUNIYAH Jurnal Matematika & Sains Jurnal Pangan dan Agroindustri agriTECH Indonesian Journal of Cancer Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Indonesian Journal of Chemistry Akuatik: Jurnal Sumberdaya Perairan Jurnal Perikanan Universitas Gadjah Mada Biosaintifika: Journal of Biology & Biology Education Indonesian Journal of Natural Pigments Cermin Dunia Kedokteran Jurnal Ilmiah Berkala: Sains dan Terapan Kimia Indonesian Journal of Cancer Jurnal Buana Pengabdian JURNAL REKAYASA KIMIA & LINGKUNGAN
Leenawaty Limantara
Universitas Pembangunan Jaya
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Journal : Microbiology Indonesia

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Exploration, Isolation and Quantification of β-carotene from Bacterial Symbion of Acropora sp. NAELY K. WUSQY; LEENAWATY LIMANTARA; FERRY F KARWUR
Microbiology Indonesia Vol. 8 No. 2 (2014): June 2014
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (257.52 KB) | DOI: 10.5454/mi.8.2.3

Abstract

In the microbial world, pigments are one of the most conspicuous traits. Marine bacteria associated with Acropora sp. collected from Taka Cemara, Karimunjawa Islands were screened for the production of a yellow pigment. The isolation of bacterial symbionts from Acropora sp. on Zobell 2216E medium resulted in one bacterium, KJ5, positively synthesized carotenoids. By reverse phase HPLC analysis, one peak of the pigment types was identified as a β-carotene peak which appeared at 60.24 min. Then, sample of the β-carotene was collected and identified according to their spectral characteristics and compared with the published data in different types of solvent. Based on the HPLC analysis, the total β-carotene contents were calculated by converting the broad absorption of β-carotene. Molecular identification of the bacterium KJ5 using 16S rDNA showed that bacterium KJ5 was closely related to Erythrobacter flavus with 96% homology value.
Co-Authors A. B. Susanto A.B. SUSANTO AB Susanto Adhiwibawa, Marcelinus A.S. Agus Sabdono Aji Wahyu Budiyanto Amelia Myristi Lolita Antonius Budi Susanto Ayu Nur Aida Bambang Cahyono Budi Prasetyo C Hanny Wijaya Christina Astutiningsih Dece Elisabeth Sahertian Diah Mustika Lukitasari Diah Mustika Lukitasari, Diah Mustika Dian Resita Dian Ridwan Nurdiana Dwi I Prayitmo DWI SUSILANINGSIH EDWIN MAHENDRA Elly Natalina Endrika Widyastuti Ferdiansyah Ferdiansyah Ferry Fredy Karwur Fitriyah Nurhidayah Gabriela Johana Septiany, Gabriela Johana Hana Susanti Maleta Hari Kristopo Helly de Fretes Heriyanto . Heriyanto Heriyanto Heriyanto Heriyanto Heriyanto Heriyanto Heriyanto Heriyanto Heriyanto Heriyanto Heriyanto Heriyanto Heriyanto Heriyanto Heryanto Heryanto Hong, Khoo Mun Indriatmoko Indriatmoko Jaya Mahar Maligan Junet. F. da Costa Katarina Purnomo Salim Lia Kusmita M. Rio Syahputra Madalena Madalena Maleta, Hana Susanti Maria Ika Putri Soegiarto, Maria Ika Putri Muhammad Diah Khoirul Huda Naely K Wusqy NAELY K. WUSQY Naomi Megananda Mulyadi Naomi Megananda Mulyadi, Naomi Megananda Naurissa Biasini Naurissa Biasini Neltji Herlina Ati Novita Wijayanti Nur Ida Panca Nugrahini Ocky K Radjasa Ocky K. Radjasa Ocky K. Radjasa Ocky Karna Radjasa Prasetyandaru Pirenantyo Prasetyandaru Pirenantyo, Prasetyandaru Pratika Riris Putrianti Pratika Riris Putrianti Puji Rahayu Puji Rahayu Rebecca Christiana Reni Dyanasari Reni Dyanasari Reni Subawati Kusumaningtyas Renny Indrawati Renny Indrawati Renny Indrawati, Renny Reny Pratiwi Rosita Dwi Chandra Rosita Dwi Chandra, Rosita Dwi Salim, Katarina Purnomo Sitepu, Rehmadanta Soenarto Notosoedarmo Soenarto Notosudarmo Sulistyowati Sulistyowati Suryasatriya Trihandaru Susanti Pudji Hastuti Tatas H.P. Brotosudarmo Teti Estiasih Tri Dewanti Widyaningsih Venny Santosa Widya Dwi Rukmi Putri Windu Merdekawati Yundari, Yundari _ Heriyanto