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All Journal HAYATI Journal of Biosciences Biospecies Jurnal Natur Indonesia POSITRON AL KAUNIYAH Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biogenesis: Jurnal Ilmiah Biologi Prosiding Seminar Biologi Journal of Aceh Physics Society BIO-SITE |BIOLOGI Sains Terapan Jurnal Riau Biologia Jurnal Biologi Tropis Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains Minda Baharu Logista: Jurnal Ilmiah Pengabdian Kepada Masyarakat Jurnal Abdimas PHB : Jurnal Pengabdian Masyarakat Progresif Humanis Brainstorming Unri Conference Series: Community Engagement
Tetty Marta Linda
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Agriculture, Biological Sciences & Forestry Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mechanical Engineering Physics Public Health Other

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Identification and Classification of Thiamine with Amperometric Biosensor Based on Saccharomyces cerevisiae and Principal Component Analysis Umar, Lazuardi; Rosandi, Vira Annisa; Nainggolan, Leonardus Riski; Setiadi, Rahmondia Nanda; Linda, Tetty Marta
POSITRON Vol 13, No 1 (2023): Vol. 13 No. 1 Edition
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam, Univetsitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/positron.v13i1.59432

Abstract

Vitamin B1 (thiamine) plays an important role in various metabolic processes and is one of the main factors in the body's health. Thiamine excess and deficiency in the body might cause a variety of diseases such as shortness of breath, beriberi, and heart failure, hence thiamine levels must be detected as a reference for intake. The present research detected thiamine using the amperometric biosensor method and the yeast Saccharomyces cerevisiae as a bioreceptor. Dissolved oxygen (DO) level measurement parameters are considered based on yeast cell metabolism. Thiamine was treated with various concentrations of 15 mM, 30 mM, 45 mM, 60 mM, and 75 mM. The measurement results are in the voltage range of 1912–1964 mV, where the addition of thiamine causes an increase in the reproducibility and growth rate of yeast cells. The ideal characteristics of the biosensor based on the parameters of sensitivity, linearity, and stability was also carried out, which resulted in successive biosensor measurements of 0.925 mV/mM, a correlation coefficient of r = 0.9868, and a decrease in the voltage response of the biosensor up to 4.97% from its initial activity. The measured data were grouped and classified using principal component analysis (PCA), which resulted in a total accumulated data variance percentage of 84.5% and an eigenvalue > 1 for both PCs. It is intended that the findings of this research can be utilized as a reference for controlling vitamin consumption levels that have an impact on health.
Quantitative Caffeine Analysis in Robusta Coffee Utilizing Amperometric Biosensing Technology Rosandi, Vira Annisa; Umar, Lazuardi; Setiadi, Rahmondia Nanda; Rini, Ari Sulistyo; Erwin, Erwin; Yanuar, Yanuar; Linda, Tetty Marta
POSITRON Vol 13, No 2 (2023): Vol. 13 No. 2 Edition
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam, Univetsitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/positron.v13i2.70008

Abstract

Consuming caffeine in inappropriate amounts can disrupt various aspects, especially health. Controlling intake by knowing the caffeine levels in coffee is necessary to reduce the potential negative impacts. This research focuses on the detection of caffeine in Robusta coffee at two different concentrations (1:10 and 1:20 g/mL) and its relationship with yeast metabolism. An amperometric biosensor with a transimpedance amplifier to measure caffeine levels is used which has the advantages of sensitivity, cost-effectiveness, real time monitoring, biocompatibility, and reliable measurements. The data were statistically analyzed using ANOVA and visualized using Principal Component Analysis (PCA). The results revealed a concentration -dependent decrease in biosensor readings as caffeine levels increased (0.1, 0.5, 1, 1.5, and 2 mM), indicating caffeine's ability to inhibit yeast oxygen consumption and oxygen-dependent metabolic processes. The sensitivity of the biosensor in detecting caffeine is 36.66 mV/mM. PCA uncovered complex patterns, relationships, and variations within the caffeine data. PC1 and PC2, the first two principal components, collectively explained 86.3% of the data's variance. Eigenvalues for both PCs were greater than 1, highlighting their significance in understanding the dataset's complexity. This research enhances our understanding of caffeine content in Robusta coffee and its effects on yeast metabolism, providing valuable insights for the coffee industry. This use of yeast biosensors offers efficiency, and adaptability that make that biosensor valuable in a variety of scientific and industrial contexts.
Potensi Bacillus spp. Sebagai Penghasil Biosurfaktan untuk Pengolahan Limbah Minyak Pelumas Zia, Khaleda Zia; Linda, Tetty Marta
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 8, No 2 (2023): June 2023
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v8i2.6360

Abstract

Biosurfaktan adalah makromolekul ekstraseluler yang dihasilkan oleh mikroorganisme yang memiliki sifat ramah lingkungan serta memiliki berbagai fungsi salah satunya untuk emulsifikasi limbah minyak. Penelitian ini bertujuan mengetahui potensi isolat Bacillus spp. dalam menghasilkan senyawa biosurfaktan untuk pengolahan limbah minyak pelumas. Metode yang digunakan adalah penetapan indeks emulsifikasi dan analisis aktivitas emulsifikasi (drop collapse test, oil spreading technique, uji tegangan muka) dari supernatan kultur Bacillus spp., serta karakterisasi biosurfaktan dengan Kromatografi Lapis Tipis (KLT). Supernatan dari ketiga isolat Bacillus spp. menunjukkan hasil positif dengan uji drop collapse test dan oil spreading technique dengan diameter zona bening tertinggi diperoleh dari Bacillus sp 48 sebesar 10,2 mm, serta nilai indeks emulsifikasi (E24) sebesar 99,5%. Berdasarkan hasil KLT diketahui bahwa biosurfaktan yang dihasilkan oleh ketiga isolat bakteri Bacillus spp. adalah golongan lipopeptida. Bacillus spp. yang dapat menurunkan tegangan permukaan dari minyak pelumas adalah Bacillus sp 48 yaitu sebesar 19,7 dyne/cm, Bacillus sp 34 sebesar 17,9 dyne/cm dan Bacillus sp 84 sebesar 15,7 dyne/cm. Biosurfaktan dari ketiga isolat Bacillus spp. berpotensi dikembangkan dalam berbagai bidang industri. 
Endophytic bacteria isolated from stems and roots of Acrostichum aureum Linn. potential for hydrolytic enzyme and α-amylase inhibitor Linda, Tetty Marta; Defani, Syafiqah Amirah; Berliansyah, Azizul; Febriarti, Bernadeta Leni; Zul, Delita
Biogenesis: Jurnal Ilmiah Biologi Vol 12 No 1 (2024)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v12i1.41668

Abstract

Endophytic bacteria live symbiotically in plant tissues but do not hurt plants. Endophytic bacteria are widely used in the industrial sector as enzyme producers. This study aims to examine the potential of endophytic bacteria from the stems and roots of sea fern (Acrostichum aureum L.) as producers of hydrolytic enzymes and to determine their potential as α-amylase inhibitors. Macroscopic and biochemical tests characterized endophytic bacterial isolates. Hydrolytic enzyme activity test consisted of cellulase, lipase, and laccase enzyme. Isolates that were able to hydrolyze were tested for antidiabetic potential by α-amylase inhibitor test. A total of 24 bacterial isolates were selected for their ability to produce cellulase, lipase, and laccase. The results obtained 24 isolates of endophytic bacteria showed that as much as 33% of stem isolates and 52% of root isolates were able to produce hydrolytic enzymes. The α-amylase inhibition test results of the three endophytic bacteria tested were isolates A.T 2 (17%) and A.A 3 (8%) on 1% starch substrate, and A.T 2 (36%) on 2% starch substrate with a spectrophotometer at a wavelength of 540 nm. Endophytic bacteria isolated from the stems and roots of sea ferns can be developed as an alternative base material for herbal medicines for antidiabetics.
PELATIHAN PEMBUATAN SABUN ORGANIK BERBAHAN DASAR MINYAK KELAPA UNTUK PEMBERDAYAAN WARGA DI DESA BARU KABUPATEN KAMPAR Linda, Tetty Marta; Purba, Agnes Tasya; Fauzi, Tiara Nurfadila; Rahayu, Sri Jumaliza; Syahgiri, Aldiyan; Nilandra, Namira Ratu; Pangestu, Adjie; Ramadhan, Dhea Amanda; Respati, Reno Widi; Wahyuni, Levita; Fukuhara, Shafira Keiko
MINDA BAHARU Vol 7, No 1 (2023): Minda Baharu
Publisher : Universitas Riau Kepulauan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33373/jmb.v7i1.4548

Abstract

Berdasarkan data yang tertera disebutkan bahwa mayoritas mata pencaharian masyarakat Desa Baru adalah petani dan buruh, diharapkan masyarakat desa dapat mengembangkan potensinya untuk menciptakan usaha dalam bentuk UMKM, sehingga mampu untuk menunjang ekonomi dan menjadi ciri khas Desa Baru. Sabun merupakan campuran senyawa natrium dengan asam lemak yang dapat digunakan sebagai pembersih, berbentuk padat, berbusa dengan atau tanpa bahan tambahan lainnya dan tidak dapat menyebabkan iritasi pada kulit ketika digunakan. Pada pembuatan sabun organik sederhana ini menggunakan minyak kelapa sebagai bahan baku utama dan natrium hidroksida (NaOH) dengan penambahan kopi sebagai pengharum organik melalui reaksi saponifikasi. Metode pelaksanaan yang dilakukan dalam kegiatan ini antara lain: melakukan sosialisasi kegiatan terkait pembuatan sabun dan manfaatnya, mengisi kuisioner sebelum dan sesudah kegiatan sebagai tolak ukur keberhasilan kegiatan tersebut, melaksanakan praktik pembuatan sabun dan pengemasan sabun yang telah jadi. Melalui kegiatan tersebut terciptalah produk sabun berbahan dasar minyak kelapa melalui metode penyabunan yang sederhana. Produk ini akan memiliki nilai jual yang tinggi jika minat masyarakat terhadap produk tersebut juga tinggi
The Ureolytic Soil Bacteria Bacillus albus, a potential Agent for Biocement Linda, Tetty Marta; Rahmani, Syauqi Susana; Wati, Andini Saras; Febriarti, Bernadeta Leni; Futra, Dedi; Olivia, Monita; Juliantari, Erwina
HAYATI Journal of Biosciences Vol. 32 No. 3 (2025): May 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.3.829-839

Abstract

Concrete is a common building material and is very vulnerable to cracking caused by unstable temperature/humidity. Concrete crack repair can be done by using microorganism substitution that can produce CaCO3 (calcite) compounds that can be used as an environmentally friendly method in improving structural formation and increasing the strength and durability of concrete, one of which is using ureolytic bacteria. This study aimed to isolate and characterize ureolytic bacteria isolates and then to assess the calcite precipitation potential of ureolytic bacteria isolates from landfills. The ureolytic bacterial isolates were grown on NB-U/Ca and tap water medium. Analysis of Calcite Structure using Fourier Transform Infra-Red (FTIR), and molecular identification using 16S rRNA gene sequences. Bacterial isolate SP. 48 were able to grow and produce calcite in both media, especially in tap water medium. FTIR results showed that the precipitates produced by bacterial isolates on both mediums had strong absorption peaks, which were detected to be calcite. Molecular identification using the 16S rRNA gene sequence showed that the isolate is Bacillus albus. B. albus is a proteolytic bacterium collected from landfills that was proven to be a calcite-producing bacterium, a new finding in this study. B. albus can grow and produce calcite in a tap water medium with low pH. This finding can be used as an alternative to overcome concrete cracks and increase the strength and durability of concrete. This bacterial isolate could be developed as a biocement candidate.
Pengembangan UMKM Kelompok Usaha Bersama Citra Lestari Desa Tanah Merah Melalui Promosi Digitalisasi dan Branding Produk Linda, Tetty Marta; Sari, Avilia Dayang; Amanda, Dea; Husadha, Azura; Wijayanti, Indriani; Zahara, Latifah; Azzahra, Fhatya; Amalia, Rizki; Qosim, Muhammad Abdullah; Ramadhan, Fitra; Ramadhan, Ilmi Fajar
Jurnal Pengabdian Masyarakat Progresif Humanis Brainstorming Vol 6, No 2 (2023): Jurnal Abdimas PHB : Jurnal Pengabdian Masyarakat Progresif Humanis Brainstormin
Publisher : Politeknik Harapan Bersama

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30591/japhb.v6i2.4157

Abstract

Usaha Mikro Kecil Menengah (UMKM) merupakan bisnis sektor penting untuk mengembangkan ekonomi di masyarakat. Masa sekarang UMKM berkembang pesat seiring dengan meningkatnya kebutuhan ekonomi untuk menghadapi persaingan di sektor usaha. Guna menghadapi persaingan di sektor bisnis saat ini, diperlukan digitalisasi UMKM untuk mengembangkan potensi usaha jahit Kelompok Usaha Bersama (KUB) Citra Lestari di Desa Tanah Merah. Tujuan dari kegiatan pengabdian masyarakat ini adalah untuk mengembangkan UMKM KUB Citra Lestari melalui promosi dan digitalisasi serta branding produk. Metode kegiatan ini menggunakan pendekatan deskriptif dengan menggunakan platform media sosial Instagram dalam pemasaran produk dan pelabelan. Hasil dari kegiatan ini yaitu KUB Citra Lestari memiliki akun Instagram dengan username @kub_citralestari, pembuatan email, label produk dan template pengeditan foto produk melalui pengeditan Canva. Setiap karya jahit yang dihasilkan para anggota dilatih untuk mengunggah ke akun media sosial mereka sebagai salah satu bentuk promosi. Selama proses sosialisasi dan pemantauan jumlah followers pada Instagram @kub_citralestari berjumlah 135. Kegiatan ini diharapkan dapat meningkatkan dan memperluas pemesanan produk dan lebih dikenalnya KUB Citra Lestari.
Isolation and Characterization of Thermophilic Bacteria as Producing Enzyme Amylase from Kepanasan Hot Springs In Tapung Hulu, Kampar, Riau Herwanda, Fadia Rezika; Linda, Tetty Marta
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.7888

Abstract

Thermophilic bacteria are a group of bacteria that can live and survive at high temperatures ranging from 45°C-80°C.  Thermophilic bacteria are the best choice as a producer of thermostable enzymes that can endure high temperatures used in various industrial fields including the textile industry, food industry, detergent industry, and pharmaceutical industry. This study aims to isolate, characterize, and test the activity of thermostable amylase enzyme from thermophilic bacterial isolates in Kepanasan hot spring in Tapung Hulu District, Kampar Regency, Riau. Characterization of thermophilic bacterial isolates is macroscopic, physiological, and biochemical tests: Triple sugar iron agar, Sulphur indole motility, symmons citrate, and catalase. The results of study obtained ten isolates of thermophilic bacteria consisting of six isolates from hot water samples and four isolates from soil sediments isolated at temperatures of 45°C and 50°C using a nutrient agar (NA) medium. The results of Gram staining were obtained one Gram-positive isolate and nine Gram-negative isolates. Positive catalase test results, negative indole and motility tests for ten isolates. The results of the amylase enzyme activity test obtained three bacterial isolates, namely BTA 6, BTT 2 and BTT 4 with the highest amylolytic index of 1.32 produced by BTT 2 isolate at an incubation temperature of 50°C with a starch concentration of 1%.
Potensi Bacillus spp. Sebagai Penghasil Biosurfaktan untuk Pengolahan Limbah Minyak Pelumas Zia, Khaleda Zia; Linda, Tetty Marta
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 8, No 2 (2023): June 2023
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v8i2.6360

Abstract

Biosurfaktan adalah makromolekul ekstraseluler yang dihasilkan oleh mikroorganisme yang memiliki sifat ramah lingkungan serta memiliki berbagai fungsi salah satunya untuk emulsifikasi limbah minyak. Penelitian ini bertujuan mengetahui potensi isolat Bacillus spp. dalam menghasilkan senyawa biosurfaktan untuk pengolahan limbah minyak pelumas. Metode yang digunakan adalah penetapan indeks emulsifikasi dan analisis aktivitas emulsifikasi (drop collapse test, oil spreading technique, uji tegangan muka) dari supernatan kultur Bacillus spp., serta karakterisasi biosurfaktan dengan Kromatografi Lapis Tipis (KLT). Supernatan dari ketiga isolat Bacillus spp. menunjukkan hasil positif dengan uji drop collapse test dan oil spreading technique dengan diameter zona bening tertinggi diperoleh dari Bacillus sp 48 sebesar 10,2 mm, serta nilai indeks emulsifikasi (E24) sebesar 99,5%. Berdasarkan hasil KLT diketahui bahwa biosurfaktan yang dihasilkan oleh ketiga isolat bakteri Bacillus spp. adalah golongan lipopeptida. Bacillus spp. yang dapat menurunkan tegangan permukaan dari minyak pelumas adalah Bacillus sp 48 yaitu sebesar 19,7 dyne/cm, Bacillus sp 34 sebesar 17,9 dyne/cm dan Bacillus sp 84 sebesar 15,7 dyne/cm. Biosurfaktan dari ketiga isolat Bacillus spp. berpotensi dikembangkan dalam berbagai bidang industri. 
Seleksi Aktinomisetes dalam Menghasilkan Indole Acetic Acid dan Efektivitas Terhadap Perkecambahan Benih Cabai Merah (Capsicum annum L.) Anggraini, Yeyen Septia; Linda, Tetty Marta; Lestari, Wahyu
Biospecies Vol. 11 No. 2 (2018): Juli 2018
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (767.978 KB) | DOI: 10.22437/biospecies.v11i2.5746

Abstract

Indole Acetic Acid (IAA) is an important that could improve plant growth. Actinomycetes is one group of bacteria that could produce IAA. The purpose of this study was to examines the potential of actinomycetes from Siak, Riau Peatland in producing IAA the effects to red pepper (Capsicum annuum L.) germination. The result of this study was collected 21 isolates of actinomycetes which able only to produced IAA in SCB (Starch Casein Broth) medium with tryptophan addition. The produce highest IAA in L225 isolate with concentration 37,70 ± 1,00 µg/ml by medium SCB with typtophan addition. 14 isolates gave respons for length of sprouts parameters and 20 isolates gave effect to the root length parameters for red pepper seeds with incubation for 15 day.
Co-Authors , HERLINDA , TABRI Aini, Diah Nurul Amanda, Dea Anggraini, Yeyen Septia Atria Martina Azzahra, Fhatya Berliansyah, Azizul Berlyansah, Azizul BERNADETA LENI FEBRIANTI, BERNADETA LENI Binar Robinson M Cici Sri Wahyuni Dedi Futra Defani, Syafiqah Amirah Delita Zul Dessy Sari Mulfa Diah Nurul Aini Dwi Wahyuni Erwin Erwin ERWINA JULIANTARI, ERWINA Fauzi, Tiara Nurfadila Febriarti, Bernadeta Leni Fukuhara, Shafira Keiko Hanifa Hanifa Hanifa, Hanifa Herwanda, Fadia Rezika Husadha, Azura Imelda Wardani L. FIBRIARTI, BERNADETA LAMISSI NAPITUPULI Lazuardi Umar Liska Chairani Harahap MAWARTI, INDAH Maya Sari Mira Rianda Monita Olivia Mufidah Dwi Suci Ningsih Muhammad Rinaldi Mulfa, Dessy Sari Nainggolan, Leonardus Riski Nani Anggraini Nery Sofianti Nery Sofiyanti Nila Veronika Nilandra, Namira Ratu Ninik Nihayatul Wahibah Novalita Dwi Fanny Pangestu, Adjie PESRITA, ANALIA Purba, Agnes Tasya Qosim, Muhammad Abdullah Rahayu, Sri Jumaliza Rahmah Agnissah Rahmani, Syauqi Susana Rahmondia N. Setiadi Ramadhan, Dhea Amanda Ramadhan, Ilmi Fajar Rati, Yolanda Ratna Jelita REGIANA SINTAWATI, REGIANA Respati, Reno Widi Rini, Ari Sulistyo Rini, Ari Sulistyo Rizki Amalia RODESIA MESTIKA ROZA Rodesia Mustika Roza Rosandi, Vira Annisa Saberina Hasibuan Safrida Dwiningsih Sari, Avilia Dayang Sembiring, Rinawati Sezhianindi Firiola Silvera Devi Sri Ramadhani Syahgiri, Aldiyan Syauqi Susana Rahmani Tiara Elsita Masni Vanda Julita Julita Vanisa Sri Elvani Wahyu Lestari Wahyu Lestari Wahyu Lestari Wahyuliyanti, Wahyuliyanti Wahyuni, Levita Wati, Andini Saras Wijayanti, Indriani Windi Dona Fitri Fitri Yanuar Hamzah Yanuar Yanuar, Yanuar Yuliati, Rola Zahara, Latifah Zia, Khaleda Zia