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Karakterisasi Jeroan Ikan Cakalang sebagai Skrining Awal Bahan Baku Perangkap Lalat Rumah Musca domestica dan Antibakteri: Characterization of Skipjack Viscera as Initial Screening of Sources for Housefly Traps Musca domestica and Antibacterial Rahmatia Garwan; Harsi Kusumaningrum; Tati Nurhayati; Hanifah Nuryani Lioe
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 25 No 1 (2022): Jurnal Pengolahan Hasil Perikanan Indonesia 25(1)
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v25i1.38555

Abstract

Viscera of skipjack tuna which is rich in protein has the potential as a source of Musca domestica house fly trap-compounds as well as a source of antibacterial. This study aims to explore the potential of skipjack viscera in the form of stomach, intestines, liver and the mixtures as a source of house fly traps and antibacterial components. Skipjack viscera were stored for 2 days at room temperature. Moisture content, protein, TVB, amino acids, fly trap activity and antibacterial activity were then analyzed. The results showed that the water content, fat and TVB of all parts of skipjack viscera increased, while the protein content decreased during 2 days of storage. The water content ranged from 73,90% wb to 85,19% wb. Protein content was found from 14,46% wb to 18,07% wb. Fat content ranged from 0,95% wb to 2,71% wb, while TVB levels ranged from 64,74 mg N/100 g to 133,92 mg N/100 g. The main amino acid composition of skipjack viscera was glutamic acid, arginine, glycine, phenylalanine, tyrosine, lysine, serine, valine and histidine. The increase in TVB levels after 2 days of storage was associated with the highest activity of stomach viscera in trapping flies with an average of 13.50 flies. The greatest bacterial growth inhibition was showed by the mixture of viscera on day 0 at a concentration of 75% against Staphylococcus aureus and Escherichia coli, i.e., 9.30 mm and 8.78 mm zone of inhibition, respectively. The antibacterial activity against both bacteria was classified as a moderate. Flies-trapping and antimicrobial compounds are likely closely related to the amino acid composition of skipjack viscera. More in-depth research on the role of amino acids as the main component of flies-trapper and antimicrobial of skipjack viscera is the subject of further research.
Validasi Metode Analisis Kandungan Spesifik Residu Total Monomer Stiren Pada Kemasan Polistiren Dina Mariana; Nuri Andarwulan; Hanifah Nuryani Lioe
Jurnal Kimia dan Kemasan Vol. 35 No. 2 Oktober 2013
Publisher : Balai Besar Kimia dan Kemasan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24817/jkk.v35i2.1883

Abstract

Monomer stiren merupakan bahan dasar kemasan pangan yang menjadi isu perhatian terkait keamanan pangan. Saat ini di dalam peraturan nasional maupun internasional, peraturan persyaratan pada total residu dari monomer stiren dalam kemasan pangan. Dalam rangka menunjang pengawasan kemasan pangan polistiren, maka diperlukan peningkatan kapasitas pengujian kandungan spesifik residu total monomer stiren di laboratorium sesuai dengan peraturan yang berlaku. Penelitian ini bertujuan untuk melakukan validasi metode analisis pengujian kandungan spesifik residu total monomer stiren pada kemasan polistiren dengan heptana sebagai simulan pangan menggunakan kromatografi gas dengan pendeteksi ionisasi nyala, sesuai prosedur uji yang diatur dalam Peraturan Kepala Badan POM Nomor HK.03.1.23.07.11.6664 Tahun 2011 tentang Pengawasan Kemasan Pangan. Hasil validasi metode analisis adalah linieritas dengan persamaan regresi y = 0,186x nilai R2 = 0,999, presisi dengan nilai relatif standar deviasi (RSD) = 0,93 %, akurasi dengan persen perolehan kembali (% recovery) 98,04 ± 2,62 %, pada konsentrasi stiren yang ditambahkan 502 μg/g dan selektivitas yang baik. 
POLA PERTUMBUHAN ASPERGILLUS OCHRACEUS BIO 220 DAN PRODUKSI OKRATOKSIN A PADA JAGUNG DAN KEDELAI INVITRO (Growth Pattern of Aspergillus ochraceus and Ochratoxin A Production on Maize and Soybens invitro) Sinta Simatupang; Winiati P. Rahayu; Hanifah N. Lioe; Dian Herawati; Wisnu Broto; Santi Ambarwati
Jurnal Penelitian Pascapanen Pertanian Vol 11, No 1 (2014): Jurnal Penelitian Pascapanen Pertanian
Publisher : Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpasca.v11n1.2014.48-56

Abstract

Kapang toksigenik Aspergillus ochraceus penghasil mikotoksin dapat menimbulkan masalah kesehatan bila mengkontaminasi bahan pangan seperti jagung dan kedelai. Pertumbuhan kapang A. ochraceus dipengaruhi oleh perubahan iklim, seperti perubahan suhu dan kelembaban. Penelitian ini bertujuan untuk mengevaluasi pengaruh perubahan suhu dan kelembaban terhadap pertumbuhan A. ochraceus dan jumlah okratoksin A yang diproduksinya. Aspergillus ochraceus BIO 220 di diinokulasikan pada media Potato Dextrose Agar (PDA), jagung dan kedelai, kemudian diinkubasi selama 20 hari pada tiga kondisi suhu (20, 30, 40 oC) dan tiga tingkat kelembaban (70, 80 dan 90%). Pertumbuhan miselium dan spora diamati setiap dua hari dan konsentrasi okratoksin A yang terbentuk dianalisis dengan RP-HPLC yang dilengkapi dengan fluorescence detector setelah 40 hari. Pertumbuhan kapang toksigenik A. ochraceus BIO 220 pada media laboratorium PDA, jagung dan kedelai optimal pada suhu 30 oC dan kelembaban 90%. Pembentukan okratoksin A optimum pada jagung dan kedelai yang dikontaminasi dengan A. ochraceus BIO 220 pada suhu 20 oC dan RH 80% masing-masing sebanyak 93 dan 45 ppb. Kapang A. ochraceus BIO 220 tidak dapat tumbuh pada jagung dan kedelai bila kondisinya ekstrim yaitu pada suhu 40°C dengan kelembaban 70, 80 dan 90 %.Kata kunci :Aspergillus ochraceus, kelembaban, okratoksin, suhuEnglish Version AbstractToxigenic fungi, Aspergillus ochraceus producing ochratoxin A can cause serious health problem if the fungi contaminated food product such as maize and soybean. A. ochraceus growth is affected by climate change including the change of temperature and relative humidity. This study was performed to evaluate the effect of temperature and relative humidity on A. ochraceus BIO 220 growth and its ochratoxin A production. Aspergillus ochraceus BIO 220 was inoculated in Potatoes Dextrose Agar (PDA) media, maize and soybean, then incubated at 3 different temperatures (20, 30, 40 oC) dan 3 different relative humidities (70, 80 dan 90%) for 20 days. Mycelium and spores were in observed every two days and the level of ochratoxin A was analyzed using RP-HPLC equipped by fluorescence detector after 40 days. Optimum growth for A. ochraceus BIO 220 in laboratory media, maize and soybean was at temperature 30 °C and relative humidity 90%, while the highest ochratoxin A level was reached in maize (93 ppb) and soybean (45 ppb) at temperature of 20 °C and 80 % relative humidities. Aspergillus ochracheus BIO 220 could not grow in maize and soybean media at extreme condition (temperature 40 °C and relative humidity 70, 80 dan 90 %).Keywords :Aspergillus ochraceus, ochratoxin, relative humidity, temperature
Validasi Metode Analisis Kandungan Spesifik Residu Total Monomer Stiren Pada Kemasan Polistiren Dina Mariana; Nuri Andarwulan; Hanifah Nuryani Lioe
Jurnal Kimia dan Kemasan Vol. 35 No. 2 Oktober 2013
Publisher : Balai Besar Kimia dan Kemasan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1840.948 KB) | DOI: 10.24817/jkk.v35i2.1883

Abstract

Monomer stiren merupakan bahan dasar kemasan pangan yang menjadi isu perhatian terkait keamanan pangan. Saat ini di dalam peraturan nasional maupun internasional, peraturan persyaratan pada total residu dari monomer stiren dalam kemasan pangan. Dalam rangka menunjang pengawasan kemasan pangan polistiren, maka diperlukan peningkatan kapasitas pengujian kandungan spesifik residu total monomer stiren di laboratorium sesuai dengan peraturan yang berlaku. Penelitian ini bertujuan untuk melakukan validasi metode analisis pengujian kandungan spesifik residu total monomer stiren pada kemasan polistiren dengan heptana sebagai simulan pangan menggunakan kromatografi gas dengan pendeteksi ionisasi nyala, sesuai prosedur uji yang diatur dalam Peraturan Kepala Badan POM Nomor HK.03.1.23.07.11.6664 Tahun 2011 tentang Pengawasan Kemasan Pangan. Hasil validasi metode analisis adalah linieritas dengan persamaan regresi y = 0,186x nilai R2 = 0,999, presisi dengan nilai relatif standar deviasi (RSD) = 0,93 %, akurasi dengan persen perolehan kembali (% recovery) 98,04 ± 2,62 %, pada konsentrasi stiren yang ditambahkan 502 μg/g dan selektivitas yang baik. 
Evaluation of Major Fatty Acids Determination in Palm Oil by Gas Chromatography-Flame Ionization Detection Moh Taufik; Hanifah Nuryani Lioe; Nancy Dewi Yuliana
agriTECH Vol 36, No 3 (2016)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (549.026 KB) | DOI: 10.22146/agritech.16603

Abstract

The fatty acid composition of palm oil is the major factor influencing its physical and chemical properties. The purpose of this research was to evaluate the analytical performance of major fatty acids (palmitic acid, stearic acid, oleic acid, and linoleic acid) analysis in palm oil. Triglycerides of palm oil were derivatized to fatty acid methyl esters (FAMEs) by using boron trifluoride (BF3) in methanol. FAMEs were determined by gas chromatography-flame ionization detection (GC-FID) using DB-23 capillary column as stationary phase. The studied parameters were instrument performance analysis, the efficiency of fatty acid derivatization, stability of derivatized analytes, accuracy, repeatability, intra-lab reproducibility, ruggedness, and method uncertainty. The evaluation results showed the instrument linearity at a working range of 5 to 40 mg/mL marked by coefficient of determination (R2) between 0.991-0.995. Instrument limits of detection (LOD) and instrument limits of quantification (LOQ) for 4 major fatty acids analysis were 26-35 µg/mLand 86-128 µg/mL, respectively. The increase of fatty acid concentration led to the decrease of derivatization efficiency in the fatty acids analysis. The result also showed that derivatized analytes were stable during 24 h storage at freeze temperature. The average recovery values by spiking method with the spiking concentration at 50 and 90 mg/g sample were at 75-94 % for stearic and linoleic acids analysis, however those for palmitic and oleic acids analysis were considered very low (<40 %), due to their low derivatization efficiency. Repeatability and intra-lab reproducibility of 4 major fatty acids analysis were at acceptable ranges, 0.45-1.38 % and 1.15-2.03 %, respectively. Determination by varying the volume of derivatizing agent showed the rugged method. Uncertainty of repeatability (Ur) and uncertainty of reproducibility (Ur) were ranged at 1.84-9.02 mg/g and 1.40-10.65 mg/g, respectively. This method was considerably reliable for the analysis of less abundance fatty acids in palm oil, stearic and linoleic acids.
Kondisi Penyimpanan Kacang Tanah dan Potensi Cemaran Aspergillus flavus pada Pedagang Pengecer Pasar Tradisional di Wilayah Jakarta Widya Eka Prayitno; Harsi Dewantari Kusumaningrum; Hanifah Nuryani Lioe
agriTECH Vol 38, No 1 (2018)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3811.375 KB) | DOI: 10.22146/agritech.26113

Abstract

Factors affecting contamination on peanut kernel marketed in Jakarta has not been investigated yet. The purpose of the research was to investigate and evaluate the storage condition and the behavior of retailers on the way of peanuts storage and also to investigate the presence of Aspergillus flavus infection in the peanuts. The research was conducted at traditional market with 15 peanut retailers as respondents (n=15). The research stages included survey at retailer area including interview, observation, temperature and relative humidity measurements as well as peanuts analysis including moisture content, defective seeds and presence of A. flavus. The results of research revealed that the average temperature of peanut storage area at retail stalls range from 29.6 to 31.2 °C which is not in accordance with the Codex Alimentarius Commission (CAC) recommendation, while the average of storage room RH ranged between 53.6–73.1% and moisture content of peanuts of 6.23–7.86% were mostly in accordance to CAC recommendation. The percentage of damage, shrivelled and splitted seeds ranged between 3.9–19.1%, 5.4–32.3% and 0.2–8.8%, respectively. The range of mean of total molds and A. flavus were 2.5–5.6 log cfu/g and 1.3–4.0 log cfu/g, respectively. Total molds had a strong correlation to damage kernels (r = 0.74), and had a moderate correlation to the temperature (r = 0.41), moisture content (r = 0.42) and behavior of retailers, especially in cleaning the ceiling (r = 0.44) and placing the storage container (r = 0.44). The presence of A. flavus had a slight correlation to relative humidity on storage (r = 0.26), and had no significant correlation to peanuts damage and all storage conditions. ABSTRAKFaktor-faktor yang mempengaruhi cemaran aflatoksin pada biji kacang tanah atau ose di wilayah Jakarta belum pernah dilaporkan. Penelitian ini dilakukan di pasar tradisional dengan 15 pedagang pengecer kacang tanah sebagai responden (n=15). Tujuan penelitian adalah mengidentifikasi dan mengevaluasi kondisi penyimpanan dan perilaku pengecer dalam menyimpan kacang tanah serta mengidentifikasi potensi cemaran Aspergillus flavus pada kacang tanah. Tahap penelitian meliputi survei di area pengecer (wawancara, pengamatan langsung dan pengukuran suhu serta kelembaban relatif (RH) di area penyimpanan) serta analisis kacang tanah (kadar air, biji cacat, dan keberadaan A. flavus). Hasil studi menunjukkan rata-rata kisaran suhu area penyimpanan kacang tanah di kios pengecer berkisar antara 29,6–31,2 °C. Hal ini tidak sesuai dengan rekomendasi Codex Alimentarius Commission (CAC), meskipun sebagian besar rata-rata kisaran RH area penyimpanan berkisar antara 53,6–73,1% dan kadar air kacang tanah sebesar 6,23–7,86% yang sesuai dengan rekomendasi CAC. Rata-rata biji rusak, biji keriput dan biji belah ditemukan pada kisaran, berturut-turut, 3,9-19,1%, 5,4–32,3% dan 0,2–8,8%. Rata-rata total kapang dan A. flavus pada sampel kacang tanah, masing-masing, ditemukan berkisar antara 2,5–5,6 log cfu/g dan 1,3–4,0 log cfu/g. Total kapang pada sampel kacang tanah memiliki korelasi positif yang kuat dengan biji rusak (r = 0,74), dan berkorelasi positif pada tingkat sedang dengan suhu (r = 0,41), kadar air (r = 0,42) dan perilaku pengecer dalam pembersihan langit-langit kios (r = 0,44) serta penempatan wadah simpan kacang tanah (r = 0,44). Studi ini menunjukkan bahwa keberadaan A. flavus pada sampel kacang tanah berkorelasi positif lemah dengan kelembaban relatif di area penyimpanan (r = 0,26) dan tidak memiliki korelasi secara signifikan dengan biji rusak maupun semua kondisi penyimpanan lainnya.  
NMR metabolomics revealed metabolites and bioactivity variation in Torbangun leaves Plectranthus amboinicus L. with different origins Nancy Dewi Yuliana; Muhammad Anwari Sugiharto; Hanifah Nuryani Lioe; Masao Goto; Yuko Takano Ishikawa
Indonesian Journal of Biotechnology Vol 23, No 2 (2018)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (91.375 KB) | DOI: 10.22146/ijbiotech.38659

Abstract

Plectranthus amboinicus has been reported to have antidiabetic and antioxidant activities. Environmental factors might influence the plant’s secondary metabolite profile and its beneficial properties. NMR-based metabolomics was used to show phytochemical variations between specimens of P. amboinicus grown in Japan and Indonesia. The results showed that flavonoids and triterpenes were among the discriminating factors of the variation between the two groups. Targeted comparative analysis of the concentration of the specific flavonoids of the plants using a validated HPLC-MWD method showed that the Japanese samples contained a higher concentration of total flavonoids compared with the Indonesian samples. The Japanese and Indonesian samples contained 1100.6 ± 5.1 and 532.4 ± 1.8 µg/g luteolin, and 584.5 ± 7.4 and 571.7 ± 11.6 µg/g apigenin, respectively. Eriodyctiol was detected only in the Indonesian samples. Contrarily, more intensive DPPH reduction and α-glucosidase inhibition activities were found in the Indonesian samples (IC50 14.4 ± 1.2 and 24.0 ± 0.3 µg/mL for the DPPH assay, 1181.9 ± 113.5 and 4451.4 ± 290.0 µg/mL for α-glucosidase inhibition, respectively). Thus, flavonoids might not be the only group of compounds related to the aforementioned bioactivities. This should be confirmed by further research targeting other groups of compounds, such as triterpenes.
Cultivation of Pleurotus Environtmental Friendly by Recycling Substrate Waste of Fungus and Organic Fertilizer Addition KULTIVASI JAMUR Pleurotus RAMAH LINGKUNGAN DENGAN MENDAUR ULANG LIMBAH SUBSTRAT JAMUR DAN PENAMBAHAN PUPUK ORGANIK Elis Nina Herliyana; Mira Febrianti; Abdul Munif; Hanifah Nuryani Lioe
Jurnal Silvikultur Tropika Vol. 6 No. 1 (2015): Jurnal Silvikultur Tropika
Publisher : Departemen Silvikultur, Fakultas Kehutanan dan Lingkungan, Institut Pertanian Bogor (IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/j-siltrop.6.1.%p

Abstract

White mushroom or Pleurotus ostreatus (Jacq:Fr) Kummer is one of wearthered wood that commonly found in nature. Some kinds of wood fungi, included white mushroom have been generally marketed in fresh form or in its cultivation such fungi chips. Substrate that commonly used for development of mushroom culture is saw powder of sengon wood. The more the cultivation of fungus and waste of the substrate is a lot of wasted, while its waste still has nutrition that can be recycled and reused for culturing mushroom, so that will be ecosystem friendly. The aim of this study is to learn about effect of application substrate waste of mushroom and addition of organic fertilizer on yield of white mushroom culture. Stages on this study are the making of substrate, spawning, maintenance and observation of fungus development, and analysis of chemical composition. Conducted observations are growth vegetative phase and reproductive phase, morphological character of body fruit, and result of chemical composition analysis of white mushroom body fruit. Medium composition 75% waste of mushroom substrate are mixed with 25% new substrate, showed the good growth and good yields. Addition of liquid organic fertilizer [0.2%] and [0.5%] gave the good yields. Moreover, medium and genetic factors, environmentral factor specially temperature and moisture really affected growth and the yields of white oyster mushroom.Key words: fungus culture, mushroom, Pleurotus, liquid organic fertilizer,
Identifikasi Atribut Rasa dan Aroma Mayonnaise dengan Metode Quantitative Descriptive Analysis Dwi Rahmawati; Nuri Andarwulan; Hanifah Nuryani Lioe
Jurnal Mutu Pangan : Indonesian Journal of Food Quality Vol. 2 No. 2 (2015): Jurnal Mutu Pangan
Publisher : Department of Food Science and Technology (ITP), Faculty of Agricultural Technology, Bogor Agricultural University (IPB) in collaboration with the Indonesian Food and Beverage Association (GAPMMI), the National Agency of Drug and Food Control, and th

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Abstract

Quantitative descriptive analysis (QDA) is a descriptive sensory technique commonly used to develop sensory description of a food product with some attributes and their references together with specific scores. Trained panelists were involved in the analysis. The QDA analysis consists of focus group discussion (FGD), panelists training, and sensorial evaluation. This study determined sensory attributes of mayonnaise and their references by QDA. Eleven sensorial attributes of mayonnaise were obtained by FGD. The aroma attributes were identified as lemon aroma, eggy aroma, mustard aroma, and oily aroma. The taste attributes were salty taste, umami taste, sweet taste, sour taste, eggy taste, mustard taste and oily taste. The result of sample evaluation by QDA showed that oily aroma and oily taste gave strong attributes on mayonnaise. 
Karakteristik Fisikokimia dan Sensori Mayonnaise pada Berbagai Komposisi Asam Lemak dari Penggunaan Minyak Nabati Berbeda Hanifah Nuryani Lioe; Nuri Andarwulan; Dwi Rahmawati
Jurnal Mutu Pangan : Indonesian Journal of Food Quality Vol. 5 No. 1 (2018)
Publisher : Department of Food Science and Technology (ITP), Faculty of Agricultural Technology, Bogor Agricultural University (IPB) in collaboration with the Indonesian Food and Beverage Association (GAPMMI), the National Agency of Drug and Food Control, and th

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Abstract

Mayonnaise is an oil in water emulsion and used as condiment. Different vegetable oils used for mayonnaise production give different fatty acid compositions. The objective of this study was to determine physicochemical and sensory properties of mayonnaise in the use of different vegetable oils, by analyzing pH, color and viscosity as well as aroma and taste profiles by quantitative descriptive analysis. Sesame oil, palm oil, coconut oil, soybean oil, sunflower oil, a mixture (1:1)of coconut oil and sunflower oil or palm oil and soybean oil were used in formulations to give different compositions of polyunsaturated fatty acids (PUFA), monounsaturated fatty acids (MUFA) and saturated fatty acids (SFA). Mayonnaise characteristics including pH, color (L) and viscosity values which were ranged at 3.12–3.87, 87.47–89.58 and 3700–5513 cp respectively, were less associated to different fatty acid compositions. This also happened to sensory profiles. However principal component analysis result could map SFA/MUFA/PUFA with different characteristics of mayonnaise color, aroma and taste. Mayonnaise samples which correlated to PUFA had lighter color, and characteristics of oily taste and oil aroma, with the use of sunflower oil, meanwhile samples of soybean oil which also correlated to PUFA had the characteristics of mustard aroma, savory taste, acid taste, eggy aroma and mustard taste. MUFA correlated to sweet taste. Characteristics of lemon aroma was close to SFA with the use of coconut oil or its mixture. Therefore, color, aroma and taste of mayonnaise can be affected by the use of different fatty acid compositions.
Co-Authors Abdul Choliq Abdul Munif Agustina, Lenny Aida Fadhilah Anastasia Fitria Devi Annisa Defriana Anuraga Jayanegara Anuraga Jayanegara Arum Safriana Dewi Badrut Tamam Betty S. L. Jenie Budi Nurtama C Hanny Wijaya Chalisya, Nadiah Cony Arisya Putri Dahrul Syah Dede Robiatul Adawiyah Dedi Fardiaz Dian Herawati Dian Muzdalifah Diana Ayu Nindita Dias Indrasti Dina Mariana Dina Mariana Dodik Briawan Dwi Rahmawati Dwi Rahmawati Dwiyitno Dwiyitno Dwiyitno Dwiyitno Elis Nina Herliyana Eni Kusumaningtyas Faiza A Dali Farida Ariyani farida ariyani Feri Kusnandar Hari Eko Irianto Hari Eko Irianto Harsi D. Kusumaningrum Irma Isnafia Arief Istiqamah Istiqamah Jan Schwarzbauer Jan Schwarzbauer Kurnianto, Muhammad Alfid Laras Cempaka Larissa Dsikowitzky Larissa Dsikowitzky Leonie Margaretha Widya Pangestika, Leonie Margaretha Widya Lilis Nuraida Madaniyah, Siti Maggy T. Suhartono Maggy T. Suhartono Maggy Thenawidjaja Suhartono Masao Goto Maya Saputri Melzer, Guido Mira Febrianti Muhammad Anwari Sugiharto Muhammad Hanafi N. Nurjanah Nancy Dewi Yuliana Neny Mariyani Ni Wayan Triwulandhari Nina Artanti Noer Laily Nuri Andarwulan Palupi, Nurheni Sri Puspo Edi Giriwono Rahmatia Garwan Ririn Anggraeni Rizaludin Nur Rizki Maryam Astuti Santi Ambarwati Sinta Simatupang Siti Madaniyah Sobir Sobir Sri Sugiwati Sugoi Marsaputra Karsodimejo Suliantari Suliantari SULISTIANI SULISTIANI Suyanto Suyanto TATI NURHAYATI Tatik Khusniati Taufik, Moh Tika Setianingrum Tuti Rostianti Maulani Warsono El Kiyat Weinreich, Bernd Widya Eka Prayitno Widya Puspantari Winiati P. Rahayu Winiati P. Rahayu Wisnu Ananta Kusuma Wisnu Broto Yane Regiyana Yeni Restiani Yuko Takano Ishikawa Yuli Maulidiyah Yulina Lailatul Maslukhah Zatil Afrah Athaillah Zulaikhah Zulaikhah Zulaikhah Zulaikhah, Zulaikhah