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Optimasi Formula Sari Edamame dengan Proses Pasteurisasi Berdasarkan Karakteristik Kimia dan Sensori Rizaludin Nur; Hanifah Nuryani Lioe; Nurheni Sri Palupi; Budi Nurtama
Jurnal Mutu Pangan : Indonesian Journal of Food Quality Vol. 5 No. 2 (2018)
Publisher : Department of Food Science and Technology (ITP), Faculty of Agricultural Technology, Bogor Agricultural University (IPB) in collaboration with the Indonesian Food and Beverage Association (GAPMMI), the National Agency of Drug and Food Control, and th

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Abstract

Edamame is a type of white soybeans from Japan but has been cultivated in Indonesia. It is commonly harvested as immature green soybeans. One of its products is edamame milk. This research is aimed to determine the optimized condition for edamame milk formula in terms of isoflavones, total phenolics, antioxidant activity and sensory characteristics (color, taste, aroma and overall) and to verify the optimized formula at optimum conditions according to the suggested result. The research was conducted in four stages. At 1st stage, pasteurization process at 72⁰C for 15 seconds was selected for edamame milk formula, based on hedonic test result. Optimization at the second step indicated that the optimum formula obtained was 1:6 ratio edamame:water and 0.20% food additive concentration for emulsifier and stabilizer. At the third stage, the verification results showed the product had matched with the prediction values: antioxidant activity at 1.61 mg ascorbic acid/100g, isoflavone content at 41.94 µg/g, total phenolics at 99.92 mg GAE/100 mL and acceptable organoleptic properties (scale 1-7) which gave color at 5.88, taste at 4.69, aroma at 5.36 overall at 5.23. At the four stage, comparison with commercial soybean milks, exhibited that edamame milk had higher content of antioxidant activity, isoflavones and total phenolics.
Free Glutamate Intake from Foods Among Adults: Case Study in Bogor and Jakarta Lilis Nuraida; Siti Madaniyah; Nuri Andarwulan; Dodik Briawan; Hanifah Nuryani Lioe; Zulaikhah Zulaikhah
Jurnal Mutu Pangan : Indonesian Journal of Food Quality Vol. 1 No. 2 (2014): Jurnal Mutu Pangan
Publisher : Department of Food Science and Technology (ITP), Faculty of Agricultural Technology, Bogor Agricultural University (IPB) in collaboration with the Indonesian Food and Beverage Association (GAPMMI), the National Agency of Drug and Food Control, and th

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Abstract

Monosodium glutamate (MSG) is a flavor enhancer which has been used for nearly a century to bring out the best avor of food. Its principal component is an amino acid called glutamate or L-glutamic acid. Free glutamate also exists naturally in foods. The aim of the present study was to estimate the exposure of consumers to free glutamates from foods in Jakarta and Bogor, Indonesia. The study was conducted in Jakarta urban area and rural area of Bogor with 222 respondents above 19 years of age. The survey used Food Frequency Questionnaire to estimate the consumption of food predicted to contain free glutamate. The data of food consumption was used to design food samples to be taken from the survey site and analyze for free glutamate content. Analyses of free glutamate content in food were conducted using HPLC with fluorescent detector. The results revealed the most frequent and the highest amount of food consumed both in Jakarta and Bogor area was dish menus of cereal categories. The average food consumption, excluding rice, in Bogor was 816.73 g/cap/day, while in Jakarta was 823.82 g/cap/day with dish menus contribution accounted to more than 70%. Free glutamate content of food samples ranged from undetected to more than 6 mg/g. Free glutamate intake in Bogor was comparable with that of Jakarta, i.e. 2013.76 mg/cap/day and 2068.97 mg/cap/day respectively. The main source of glutamate intake in both in Bogor and Jakarta was dish menus contributing to more than 80% of the total free glutamate intake. Free glutamate intake from food prepared outside the household was comparable with that of food prepared at home. 
Reduksi Purin pada Emping Melinjo Melalui Pre-treatment Perendaman Emping Mentah Hanifah Nuryani Lioe; Dahrul Syah; Annisa Defriana
Jurnal Mutu Pangan : Indonesian Journal of Food Quality Vol. 6 No. 2 (2019)
Publisher : Department of Food Science and Technology (ITP), Faculty of Agricultural Technology, Bogor Agricultural University (IPB) in collaboration with the Indonesian Food and Beverage Association (GAPMMI), the National Agency of Drug and Food Control, and th

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jmpi.2019.6.91

Abstract

Melinjo chips (emping as local name) has high purine content which can be related to the high uric acid level in blood that lead to joint inflamation due to the sendimentation of uric acid. The objective of this research is to prove that soaking process can reduce purine content in emping and to know the effect of the soaking process length in purine reduction. Soaking time (2, 5, and 12 hours) and emping brand (Sriti, A1 and Koki) were used as the treatments. Purine compounds such as adenine and hypoxanthine were analyzed by using High Performance Liquid Chromatography (HPLC) method with C18 column and UV detector. The three emping samples were also analyzed by using sensory evaluation to know the consumer preference toward the treated fried emping. Results obtained from this research were that soaking process can reduce both adenine and hypoxanthin content in emping. Soaking for 2 hours in water could reduce adenine content 13 – 39% and hypoxanthine content 4 – 60%. Soaking process for 2 hours on emping could reduce up to 50% of total purine base content in A1 brand, however, soaking time was not directly proportional to the decrease in purine levels. Purine content after 5 and 12 hours soaking tend to be fluctuative. Emping samples that were soaked in the water have a decrease in preference by the panelists either in color, aroma, taste, texture or in overall, but still accepted by panelists.
Formulasi Campuran Bahan Pengemulsi untuk Bolu Sponge Hanifah N. Lioe; Aida Fadhilah; Istiqamah Istiqamah
Jurnal Mutu Pangan : Indonesian Journal of Food Quality Vol. 7 No. 1 (2020): Jurnal Mutu Pangan
Publisher : Department of Food Science and Technology (ITP), Faculty of Agricultural Technology, Bogor Agricultural University (IPB) in collaboration with the Indonesian Food and Beverage Association (GAPMMI), the National Agency of Drug and Food Control, and th

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jmpi.2020.7.1.7

Abstract

To make a sponge cake, a mix of emulsifiers provides an improved aeration. The aim of this research was to formulate the mix of distilled monoglycerides (DMG) and polyglycerol esters (PGE), which had yellowish color, in a gel form for its application in sponge cake. The formula of the gel consists of primary and secondary food additives: 15.3% DMG, 6.5% PGE, 25.8% other emulsifiers and carrier including colorings (ponceau and tartrazine), 26.1% sorbitol, and 26.3% water. DMG and PGE had different colors from their expected standard color, white. The formula of DMG (15.3%) and PGE (6.5%) mix, comprising 21.8% of the formula above, was re-formulated by 100:0, 75:25, 50:50, 25:75, and 0:100 ratios of DMG to PGE. The physical characteristics of the formula consisting of color characteristic by chromameter, sensory score by R-Index method and gel spreadability were compared to the formula using standard materials of DMG and PGE. The formula was then applied for sponge cake. The specific gravity of sponge cake batter, height of sponge cake after baking, as well as sensory characteristics of crumb structure and crumb color of sponge cake were analyzed. The results of physical characterization of formula revealed that the use of 100% of PGE and the use of DMG up to 50% did not significantly differ from the standard formula. The combination of DMG and PGE formula did not affect batter specific gravity, cake height and crumb structure, but affected color of the crumb.
Penapisan Actinobacteria Akuatik Penghasil Antibakteri dari Ikan Bandeng (Chanos chanos) dan Belanak (Mugil cephalus) dengan Metode Double-Layer Diffusion Muhammad Alfid Kurnianto; Harsi Dewantari Kusumaningrum; Hanifah Nuryani Lioe
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 15, No 1 (2020): Juni 2020
Publisher : Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (946.406 KB) | DOI: 10.15578/jpbkp.v15i1.647

Abstract

Saluran pencernaan (terutama usus) ikan perairan estuaria merupakan salah satu ceruk lingkungan potensial Actinobacteria yang belum tereksplorasi. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi karakteristik morfologi Actinobacteria asal ikan bandeng (Chanos chanos) dan belanak (Mugil cephalus) serta mengevaluasi aktivitas antimikroba yang dihasilkannya. Penelitian ini diawali dengan mengambil usus ikan, kemudian digesta usus secara perlahan dipisahkan untuk dieksplorasi keberadaan Actinobacteria dengan menggunakan media isolasi selektif. Isolat yang diperoleh dikarakterisasi berdasarkan ciri makroskopik dan mikroskopik, serta dilakukan penapisan antibakteri awal menggunakan metode double-layer diffusion. Isolat dengan zona penghambatan terbaik dipilih untuk dilakukan produksi dan ekstraksi senyawa antibakteri, serta uji aktivitas antibakteri dengan metode difusi cakram terhadap bakteri uji Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa dan Escherichia coli. Sebanyak 44 isolat Actinobacteria telah diisolasi dari digesta usus ikan bandeng (Chanos chanos) dan belanak (Mugil cephalus) menggunakan media strach casein dan actinomycete isolation agar. Sebagian besar isolat yang diperoleh menunjukkan karakteristik morfologi genus Streptomyces sp., seperti koloni memiliki tekstur menyerupai serbuk, bertepung dan kasar, memiliki aerial miselium berwarna putih dan substrat miselium berwarna krim susu, serta memiliki bentuk rantai spora rectus-flexibilis. Proses penapisan antibakteri isolat Actinobacteria menunjukkan 22 isolat memiliki indeks penghambatan terhadap sedikitnya satu bakteri uji, dengan aktivitas terbaik ditunjukkan oleh isolat A-SCA-11. Uji antibakteri terhadap ekstrak kasar isolat A-SCA-11 menunjukkan aktivitas antibakteri berspektrum luas yang mampu menghambat seluruh bakteri uji dengan zona hambat tertinggi pada P. aeruginosa.  AbstractThe gut of estuary fish is one of the potential novel niches of Actinobacteria that has not yet been explored. This study aimed to isolate and identify the morphological characteristics of Actinobacteria from milkfish (Chanos chanos) and blue-spot mullet fish (Mugil cephalus) and to evaluate the antibacterial activity produced. This research was started by taking the fish gut, and then the digesta were slowly separated to explore the presence of Actinobacteria using selective isolation media. The isolates obtained were characterized by macroscopic and microscopic characteristics, and antibacterial preliminary screening of isolates was performed using a double-layer diffusion method. The isolates with the best inhibition zone were selected for production and extraction of antibacterial compounds, and antibacterial activity tests using the disk-diffusion method against the test bacteria Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa, and Escherichia coli. A total of 44 isolates of Actinobacteria have been isolated from the gut of fish using starch casein and actinomycete isolation agar. Most isolates showed morphological characteristics of the genus Streptomyces sp., such as colonies with a tough or powdery texture, antibacterial have white aerial mycelium and milk-cream substrate mycelium, and rectus-flexibilis spore chain. The antibacterial preliminary screening of Actinobacteria isolates showed 22 isolates had inhibitory index against at least one test bacterium, with the best activity indicated by A-SCA-11. Antibacterial test of A-SCA-11 crude extract showed broad-spectrum antibacterial activity that was able to inhibit all test bacteria with the highest inhibitory zone on P. aeruginosa.
Non-Target Screening Method for the Identification of Persistent and Emerging Organic Contaminants in Seafood and Sediment from Jakarta Bay Dwiyitno Dwiyitno; Larissa Dsikowitzky; Nuri Andarwulan; Hari Eko Irianto; Hanifah Nuryani Lioe; Farida Ariyani; Jan Schwarzbauer
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 10, No 3 (2015): December 2015
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v10i3.175

Abstract

Identification of persistent and emerging organic contaminants in green mussels (Perna viridis), various fishes, banana shrimp (Penaeus merguiensis) and sediment samples from Jakarta Bay has been employed. A non-target GC-MS screening approach has identified more than 60 individual organic compounds from the whole fractions either non-polar, semi-polar or acidic-polar compounds. The substances comprised as persistent organic pollutants (POPs) including DDT (dichlorodiphenyl trichloroethane) and its metabolites as well as high molecular weight polycyclic aromatic hydrocarbons (HMW-PAHs). Noteworthy, a number of emerging contaminants detected in the present study have never been reported previously either from the same location or from Asian waters. They include some priority contaminants of non-persistence halogens and emission of technical products, such as di-iso-propylnaphthalenes (DIPNs) dichlorobenzene (DCB), dichlorodiphenyl chloroethene (DDMU) and phenylmethoxynaphthalene (PMN). In general, the concentration order of the priority organic contaminants was sediment green mussel fishes shrimp. Further analysis based on the spatial distribution, individual concentrations and bioavailability suggested that some contaminants are applicable as molecular marker for the assessment of anthropogenic emission in Jakarta Bay, i.e. DIPNs, linear alkylbenzenes (LABs), phenylmethoxynaphthalene (PMN), PAHs, dichlorobenzene, DDT and its metabolites.
Potential Risk of Organic Contaminants to The Coastal Population Through Seafood Consumption from Jakarta Bay Dwiyitno Dwiyitno; Nuri Andarwulan; Hari Eko Irianto; Hanifah Nuryani Lioe; Larissa Dsikowitzky; Farida Ariyani; Jan Schwarzbauer
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 12, No 3 (2017): December 2017
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v12i3.289

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A comprehensive study on exposure assessment of the priority organic contaminants via seafood consumption has been conducted to the coastal population of Jakarta Bay. Seafood is essential food source in Indonesia and also important income for the majority of coastal populations. A number of 152 respondents from 4 districts surounding the bay were interviewed to record their frequency and pattern on seafood consumption. In the same time, 13 seafood species were collected directly from Jakarta Bay during the dry and wet seasons for the assessment of organic contaminants. A non-target GC/MS screening identified more than 40 organic contaminants in which 6 of them are potentially considered as priority contaminants including 3 groups of carcinogenic contaminants i.e. dichlorodiphenyl-trichlorethane (DDT) and its metabolites (DDXs), dichlorobenzenes (DCB) and carcinogenic PAHs (PAH4). Further exposure analysis suggested cumulative health risk of these contaminants was less than official minimal risk level (MRL) and therefore categorized safe for the corresponding population. However, attention must be paid since additional exposure of either from the different food category or other exposure route may contribute to significantly elevate the health risk on the population as well as potential exposure of emerging contaminants.
PURIFIKASI PARSIAL DAN KARAKTERISASI ß-GALAKTOSIDASE Lactobacillus plantarum B123 INDIGENOS DAN HIDROLISIS LAKTOSA UNTUK PRODUKSI SUSU ULTRA HIGH TEMPERATURE RENDAH LAKTOSA Tatik Khusniati; Neny Mariyani; Hanifah Nuryani Lioe; Didah Nur Faridah; Abdul Choliq; Sulistiani Sulistiani
Jurnal Kimia Terapan Indonesia Vol 17, No 2 (2015)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (708.319 KB) | DOI: 10.14203/jkti.v17i2.31

Abstract

β-Galactosidase is enzyme which hidrolyze lactose to glucose and galactose. This enzyme is used in production low lactose milk for consumption human which have lactose intolerance. Partial purification of β-galactosidase is important to be conducted to increase  β-galactosidase activity in order to its hydrolysis potency on UHT milk lactose increased.This research was aimed to production by partially purification and characterization indigenous β-galactosidase from Lactobacillus plantarum B123, and lactose hydrolysis for production low lactose UHT milk. Partially purification were precipitation following dialysis. Characterization included optimazion and stabilization of enzyme, while lactose hydrolisis for production low lactose UHT milk was detected by enzymatic GOD-POD kit. The results showed that production of β-galactosidase by using partial purification increased from 21.51 ± 0.23 U/mL (crude) to 106.34 ± 0.56 U/mL (dialysis).  The optimum crude β-galactosidase activity was reached in precipitation by using 60 % ammonium sulphate.  The purity of crude β-galactosidase increased 3.71 times after precipitation, and 14.28  times  after dialysis. Characterization of β-galactosidase showed that  optimum activities of crude and dialyzed β-galactosidase were at pH 6.5 and 50 oC, respectively. Stability of crude β-galactosidase incubated for 1 h were at pH: 5.0-8.5 and 25-50 °C. Specific activity of crude β-galactosidase was 15.05 U/mg protein, while that dialyzed β-galactosidase was 109.58 U/mg protein. Lactose hidrolysis to produce low lactose UHT milk showed that glucose concentration increased with the increase of hidrolysis time. Time needed to hidrolyze lactose 50 % with 4.8 U/mL β-galactosidase at 50°C was 6.08 h. In conclusion that indigenous β-galactosidase from Lactobacillus plantarum B123 purified partially can be used as lactose hidrolyzer in production of low lactose UHT milk.Key words : b-galactosidase, indigenous Lactobacillus plantarum B123, purification, lactose hidrolysis, UHT Milk
Chromatographic Profiles of Umami Fractions from Indonesian Commercial Salty Soy Sauce Hanifah Nuryani Lioe; Diana Ayu Nindita; Warsono El Kiyat
Indonesian Food and Nutrition Progress Vol 16, No 2 (2019)
Publisher : Indonesian Association of Food Technologists

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ifnp.48999

Abstract

Salty soy sauce subjected in this study is a variety of commercial soy products in Indonesia. Chromatographic profiles linked to taste dilution analysis of the soy sauce were analyzed by Sephadex G-15 gel filtration chromatography followed by RP-HPLC. The results showed that there were 4 umami fractions (Fractions I − IV) obtained by Sephadex G-15 separation. Chromatographic profiles at 254 nm could show the differentiation of the four fractions and then their RP-HPLC profiles were proven to be different from each other. Fraction III which contained 65% of the soy sauce dry matters, had the highest umami intensity with umami TD factor of 256, meanwhile, this fraction was tasted salty due to the salt contained in the soy sauce. Fraction III was dominated by the later peaks in the RP-HPLC chromatogram, which was more hydrophobic. The hydrophobic components were commonly tasted bitter, perhaps in the commercial salty soy sauce, the taste interaction between the umami and bitter components might have occurred.
Produksi β-Glukosidase Aspergillus niger BIO 2173 dengan Fermentasi Padat Menggunakan Substrat Dedak Sri Sugiwati; Maggy Thenawidjaja Suhartono; Muhammad Hanafi; Hanifah Nuryani Lioe
JURNAL SELULOSA Vol 8, No 01 (2018): JURNAL SELULOSA
Publisher : Center for Pulp and Paper

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (497.498 KB) | DOI: 10.25269/jsel.v1i01.221

Abstract

Production of β-Glucosidase Aspergillus niger BIO 2173 on Solid State Fermentation Using Rice Bran as SubstrateAbstractβ-Glucosidase (EC 3.2.1.21) is a part of the cellulase enzyme complex which acts synergistically with exoglucanase and endoglucanase to hydrolyze cellulose into glucose. The purpose of this study was to obtain the maximum fermentation conditions for production of b-glucosidase Aspergillus niger BIO 2173 with solid state fermentation using rice bran as fermentation substrate. The factors that affect the production of b-glucosidase which consist of initial pH of the fermentation medium, incubation period, ratio of water content to fermentation substrate, incubation temperature and addition of the Mandel’s mineral salts solution were examined in the study. The results showed that maximum fermentation conditions for β-glucosidase production were at initial of fermentation pH of 2,0, incubation period of 7 days, ratio of water content to substrate of 1:1, and incubation temperature of 32oC. Addition of Mandel’s mineral salts solution to the fermentation substrate at maximum fermentation conditions increased the activity and specific activity of β-glucosidase crude extract up to 5,24 ± 0,57 U/mL and 2,46 ± 0,04 U/mg, respectively.Abstrakβ-Glukosidase (EC 3.2.1.21) merupakan bagian dari enzim multi kompleks selulase, yang bekerja secara sinergis dengan eksoglukanase dan endoglukanase menghidrolisis selulosa menjadi glukosa. Tujuan dari penelitian ini adalah mendapatkan kondisi fermentasi maksimum untuk produksi β-glukosidaseAspergillus niger BIO 2173 dengan fermentasi media padat menggunakan substrat dedak. Pengujian dilakukan terhadap faktor-faktor yang mempengaruhi produksi b-glukosidase, yaitu pH awal medium fermentasi, waktu inkubasi, perbandingan kandungan air terhadap substrat medium fermentasi, suhu inkubasi dan penambahan larutan garam mineral Mandels. Hasil penelitian menunjukkan bahwa kondisi fermentasi maksimum untuk produksi b-glukosidase adalah pada pH awal medium fermentasi 2,0; waktu inkubasi 7 hari, perbandingan kandungan air terhadap substrat medium fermentasi 1:1, dan suhu inkubasi 32oC. Penambahan larutan garam mineral Mandels ke dalam substrat fermentasi pada kondisi fermentasi maksimum menyebabkan peningkatan aktivitas dan aktivitas spesifk ekstrak kasar b-glukosidase masing-masing sebesar 5,24 ± 0,57 U/mL dan 2,46 ± 0,04 U/mg protein. Kata kunci: β-glukosidase, Aspergillus niger, dedak padi, fermentasi padat, ekstrak kasar
Co-Authors Abdul Choliq Abdul Munif Agustina, Lenny Aida Fadhilah Anastasia Fitria Devi Annisa Defriana Anuraga Jayanegara Anuraga Jayanegara Arum Safriana Dewi Badrut Tamam Betty S. L. Jenie Budi Nurtama C Hanny Wijaya Chalisya, Nadiah Cony Arisya Putri Dahrul Syah Dede Robiatul Adawiyah Dedi Fardiaz Dian Herawati Dian Muzdalifah Diana Ayu Nindita Dias Indrasti Dina Mariana Dina Mariana Dodik Briawan Dwi Rahmawati Dwi Rahmawati Dwiyitno Dwiyitno Dwiyitno Dwiyitno Elis Nina Herliyana Eni Kusumaningtyas Faiza A Dali farida ariyani Farida Ariyani Feri Kusnandar Hari Eko Irianto Hari Eko Irianto Harsi D. Kusumaningrum Irma Isnafia Arief Istiqamah Istiqamah Jan Schwarzbauer Jan Schwarzbauer Kurnianto, Muhammad Alfid Laras Cempaka Larissa Dsikowitzky Larissa Dsikowitzky Leonie Margaretha Widya Pangestika, Leonie Margaretha Widya Lilis Nuraida Madaniyah, Siti Maggy T. Suhartono Maggy T. Suhartono Maggy Thenawidjaja Suhartono Masao Goto Maya Saputri Melzer, Guido Mira Febrianti Muhammad Anwari Sugiharto Muhammad Hanafi N. Nurjanah Nancy Dewi Yuliana Neny Mariyani Ni Wayan Triwulandhari Nina Artanti Noer Laily Nuri Andarwulan Palupi, Nurheni Sri Puspo Edi Giriwono Rahmatia Garwan Ririn Anggraeni Rizaludin Nur Rizki Maryam Astuti Santi Ambarwati Sinta Simatupang Siti Madaniyah Sobir Sobir Sri Sugiwati Sugoi Marsaputra Karsodimejo Suliantari Suliantari SULISTIANI SULISTIANI Suyanto Suyanto TATI NURHAYATI Tatik Khusniati Taufik, Moh Tika Setianingrum Tuti Rostianti Maulani Warsono El Kiyat Weinreich, Bernd Widya Eka Prayitno Widya Puspantari Winiati P. Rahayu Winiati P. Rahayu Wisnu Ananta Kusuma Wisnu Broto Yane Regiyana Yeni Restiani Yuko Takano Ishikawa Yuli Maulidiyah Yulina Lailatul Maslukhah Zatil Afrah Athaillah Zulaikhah Zulaikhah Zulaikhah Zulaikhah, Zulaikhah