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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan Jurnal Silvikultur Tropika (JST) Jurnal Pengolahan Hasil Perikanan Indonesia VALENSI JURNAL KIMIA SAINS DAN APLIKASI Agrointek Jurnal Penelitian Pascapanen Pertanian Jurnal Teknologi Pertanian Jurnal Kimia dan Kemasan agriTECH Indonesian Journal of Biotechnology Warta IHP (Warta Industri Hasil Pertanian) Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Jurnal Kimia Terapan Indonesia Indonesian Food and Nutrition Progress Indonesian Food Science and TechnologyJournal JURNAL SELULOSA Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Jurnal Mutu Pangan : Indonesian Journal of Food Quality Jurnal Kimia dan Kemasan Policy Brief Pertanian, Kelautan, dan Biosains Tropika Jurnal Standardisasi
Hanifah Nuryani Lioe
Departemen Ilmu Dan Teknologi Pangan, Fakultas Teknologi Pertanian, IPB University, Bogor, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Public Health Social Sciences Other

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Antibacterial activity and peptides characterization of partial-purified neutralized cell-free supernatant of bacterial isolates from pado fermented fish: Aktivitas antibakteri dan karakterisasi peptida supernatan murni parsial dari isolat bakteri asal ikan fermentasi pado Chalisya, Nadiah; Lioe, Hanifah Nuryani; Kusumaningrum, Harsi Dewantari
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 28 No. 7 (2025): Jurnal Pengolahan Hasil Perikanan Indonesia 28(7)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v28i7.63298

Abstract

Pado adalah pangan fermentasi tradisional dari Provinsi Sumatra Barat, terbuat dari ikan pelagis yang dicampur dengan ampas kelapa dan cacahan daging biji picung (Pangium edulee) kering. Bakteri asam laktat merupakan bakteri yang dominan ditemukan pada produk fermentasi dan umumnya dapat mensekresikan metabolit yang berperan dalam membantu memperpanjang masa simpan produk pangan. Supernatan bebas sel yang dinetralkan (NCFS) dari isolat tersebut diduga mengandung peptida bioaktif, yaitu peptida antibakteri. Penelitian ini bertujuan untuk menentukan NCFS murni parsial yang dihasilkan oleh isolat asal pado dengan aktivitas penghambatan terbaik terhadap Salmonella Typhimurium dan Staphylococcus aureus menggunakan metode agar difusi. NCFS kasar diperoleh dari tujuh isolat pado dengan sentrifugasi media pertumbuhannya dan menetralkan supernatannya. Sifat protein dari NCFS kasar dikonfirmasi dengan memaparkan enzim pepsin proteolitik. Ultrafiltrasi digunakan untuk memurnikan NCFS kasar secara parsial. Peptida antibakterinya dikarakterisasi menggunakan liquid chromatography-high resolution mass spectrometry (LC-HRMS). Semua NCFS menunjukkan aktivitas antibakteri terhadap S. Typhimurium, tetapi hanya tiga isolat yang menunjukkan aktivitas penghambatan terhadap S. aureus. Isolat Lactiplantibacillus plantarum B3Iw4 dipilih untuk penelitian lanjut. Fraksi NCFS dengan berat molekul rendah (dalam rentang 317,20 Da hingga 1.494,70688 Da) setelah ultrafiltrasi dengan membran 3 kDa menunjukkan peningkatan aktivitas spesifik tiga kali lipat terhadap S. aureus dibandingkan dengan NCFS kasar. Analisis LC-HRMS mengidentifikasi 20 peptida dalam NCFS murni parsial. Satu peptida, AAERAGAAALAMHGR, diprediksi memiliki aktivitas antibakteri setelah dicocokkan dengan basis data BIOPEP-UWM, dengan sekuen yang cocok AA. Peptida ini memiliki sifat hidrofobisitas yang relatif tinggi dan menarik untuk diteliti lebih lanjut.
Extraction and Physicochemical Characterization of Pectin from Noni (Morinda citrifolia) Fruit Maulani, Tuti Rostianti; Lioe, Hanifah Nuryani; Arief, Irma Isnafia; Kusumaningrum, Harsi Dewantari
Jurnal Kimia Valensi Jurnal Kimia VALENSI, Volume 10, No. 1, May 2024
Publisher : Department of Chemistry, Faculty of Science and Technology Syarif Hidayatullah Jakarta State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v10i1.37702

Abstract

Noni (Morinda citrifolia) is a popular plant in Asian countries and has great potential as pectin source for thickening agents in food products. In general, ethanol has been widely used to precipitate pectin, but the proportion can affect the quality of the product. Therefore, this study aimed to characterize the physicochemical properties of pectin from noni fruit. Extraction was performed using 0.1 N HCl at pH 1.5 and heated at 80°C for 15 min, followed by precipitation with different proportions of 96% ethanol including A (1:1), B (1:2), and C (1:3) v/v, while commercial orange pectin was used as a reference. The results showed that all pectin extracted belonged to the high methoxyl category. The FTIR spectra confirmed the presence of important functional groups including OH-hydroxyl, CH3-saturated aliphatic methyl, C=O carbonyl, and -O- cyclic ether. Furthermore, the crystalline and amorphous structures of noni pectin were confirmed by X-ray diffraction observation. The highest yield was achieved using the proportion of 1:3, followed by 1:2, and 1:1 on a wet basis. The MeO and AUA content of sample B (1:2) were relatively similar to those of the reference. Based on extraction results, noni possesses good characteristics and has the potential for commercial pectin production.
Profile of Nonvolatile Compounds and Their Correlation to the Flavor of Fried Shallots Yofananda, Olivia; Wijaya, Christofora Hanny; Lioe, Hanifah Nuryani; Sobir, Sobir
Indonesian Food Science and Technology Journal Vol. 9 No. 1 (2025): Volume 9 Number 1, December 2025 |IFSTJ|
Publisher : Department of Technology of Agricultural product (THP) Jambi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/ifstj.v9i1.41456

Abstract

This study aimed to evaluate the relationship between the nonvolatile compounds detected in fried shallots and their sensory characteristics. Six shallot varieties were used: Bima Brebes, Bauji, Tajuk, Super Philip, Batu Ijo, and Rubaru. Each variety was assessed using the rate-all-that-apply (RATA) and hedonic rating tests for the taste and aroma of fried shallots. All fried shallots were prepared in a laboratory under homogeneous frying conditions. The chemical compounds in the water extracts of the six fried shallots with molecular weights less than 3 kDa were identified using LC-MS/MS instrument. Additionally, the correlation between the chromatographic peak intensities of the chemical compounds and sensory intensities was examined through multivariate orthogonal partial least squares (OPLS) analysis. The PCA result revealed two distinct groups of samples. Batu Ijo fried shallot, which received the highest overall hedonic score and showed a strong correlation between its fragrant sulfury aroma and savory taste, was categorized separately from the others. Although 18 compounds were identified in fried shallots, a significant correlation was observed only with salty taste, primarily due to glutamyl-phenylalanine, a known kokumi compound.
FTIR Profiles, Proximate Composition, and Microbial Counts of Tauco with Sensory and Amino Acid Profiles of Ultrafiltration Fractions Herlina, Vika Tresnadiana; Lioe, Hanifah Nuryani; Adawiyah, Dede Robiatul; Kusumaningrum, Harsi Dewantari
Indonesian Food Science and Technology Journal Vol. 9 No. 1 (2025): Volume 9 Number 1, December 2025 |IFSTJ|
Publisher : Department of Technology of Agricultural product (THP) Jambi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/ifstj.v9i1.43977

Abstract

Twenty-four different tauco samples, available as commercial salt-fermented soybean products in Indonesia, were investigated for their microbial counts, proximate composition, as well as FTIR profiles obtained by attenuated total reflectance-fourier transform infrared (FTIR-ATR) instrument. The total and free amino acid profiles by UHPLC, as well as sensory characteristics by taste dilution analysis (TDA) of their ultrafiltration fractions (tauco fractions with molecular weights less than 3 kDa or <3 kDa) were also revealed. The objective of this study was to comprehensively characterize the commercial tauco products in Indonesia through integrated microbial, chemical, and sensory analyses of the products and their low molecular fractions. Tauco samples had total microbes reaching 4 to 9 log CFU/g. Lactic acid bacteria (LAB), which act in the second step of fermentation, were counted at much higher levels in all samples than molds/yeasts, which are important in the first step of fermentation. FTIR profiles of tauco samples are correlated with their protein content. Functional groups correlated with the proximate composition of tauco are identified as follows: protein with carbonyl (C=O) and aliphatic hydrocarbon (C-H), fat with carbonyl (C=O), and carbohydrate with hydroxyl (O-H) groups. Umami was identified as the predominant taste of their ultrafiltration fractions. The amino acid profiles of tauco fractions <3 kDa showed relatively high residual amino acid contents, suggesting abundant peptide content. However, multivariate orthogonal projections to latent structure (OPLS) analysis showed that free amino acids, including glutamic and aspartic acids (umami), glycine and serine (sweet), and phenylalanine and tyrosine (bitter), contributed significantly to the umami characteristic of tauco. This finding shows that free amino acids could contribute to the desirable taste of tauco. This study provides a comprehensive characterization of Indonesian tauco, offering a practical scientific reference for producers to understand product variability, improve fermentation control, and enhance the consistency and value of commercial products.
Co-Authors Abdul Choliq Abdul Munif Agustina, Lenny Aida Fadhilah Anastasia Fitria Devi Annisa Defriana Anuraga Jayanegara Anuraga Jayanegara Arum Safriana Dewi Badrut Tamam Betty S. L. Jenie Budi Nurtama C Hanny Wijaya Chalisya, Nadiah Cony Arisya Putri Dahrul Syah Dede Robiatul Adawiyah Dedi Fardiaz Dian Herawati Dian Muzdalifah Diana Ayu Nindita Dias Indrasti Dina Mariana Dina Mariana Dodik Briawan Dwi Rahmawati Dwi Rahmawati Dwiyitno Dwiyitno Dwiyitno Dwiyitno Elis Nina Herliyana Eni Kusumaningtyas Faiza A Dali Farida Ariyani farida ariyani Feri Kusnandar Hari Eko Irianto Hari Eko Irianto Harsi D. Kusumaningrum Herlina, Vika Tresnadiana Irma Isnafia Arief Istiqamah Istiqamah Jan Schwarzbauer Jan Schwarzbauer Laras Cempaka Larissa Dsikowitzky Larissa Dsikowitzky Leonie Margaretha Widya Pangestika, Leonie Margaretha Widya Lilis Nuraida Madaniyah, Siti Maggy T. Suhartono Maggy T. Suhartono Maggy Thenawidjaja Suhartono Masao Goto Maya Saputri Melzer, Guido Mira Febrianti Muhammad Alfid Kurnianto Muhammad Anwari Sugiharto Muhammad Hanafi N. Nurjanah Nancy Dewi Yuliana Neny Mariyani Ni Wayan Triwulandhari Nina Artanti Noer Laily Nuri Andarwulan Olivia Yofananda, Olivia Palupi, Nurheni Sri Puspo Edi Giriwono Rahmatia Garwan Ririn Anggraeni Rizaludin Nur Rizki Maryam Astuti Santi Ambarwati Sinta Simatupang Siti Madaniyah Sobir Sobir Sri Sugiwati Sugoi Marsaputra Karsodimejo Suliantari Suliantari SULISTIANI SULISTIANI Suyanto Suyanto TATI NURHAYATI Tatik Khusniati Taufik, Moh Tika Setianingrum Tuti Rostianti Maulani Warsono El Kiyat Weinreich, Bernd Widya Eka Prayitno Widya Puspantari Winiati P. Rahayu Winiati P. Rahayu Wisnu Ananta Kusuma Wisnu Broto Yane Regiyana Yeni Restiani Yuko Takano Ishikawa Yuli Maulidiyah Yulina Lailatul Maslukhah Zatil Afrah Athaillah Zulaikhah Zulaikhah Zulaikhah Zulaikhah, Zulaikhah