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Hanifah Nuryani Lioe
Departemen Ilmu Dan Teknologi Pangan, Fakultas Teknologi Pertanian, IPB University, Bogor, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Public Health Social Sciences Other

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Antibacterial activity and peptides characterization of partial-purified neutralized cell-free supernatant of bacterial isolates from pado fermented fish: Aktivitas antibakteri dan karakterisasi peptida supernatan murni parsial dari isolat bakteri asal ikan fermentasi pado Chalisya, Nadiah; Lioe, Hanifah Nuryani; Kusumaningrum, Harsi Dewantari
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 28 No 7 (2025): Jurnal Pengolahan Hasil Perikanan Indonesia 28(7)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v28i7.63298

Abstract

Pado is a traditional fermented food from West Sumatra, made by combining pelagic fish with grated coconut and dried seeds of Pangium edule. Lactic acid bacteria are the predominant microorganisms present and generally produce metabolites that contribute to prolonging the shelf life of the product. The neutralized cell-free supernatant (NCFS) of these isolates may contain bioactive peptides, such as antibacterial peptides. This study aimed to obtain partially purified NCFS from pado isolates with the best inhibitory activity against Salmonella typhimurium and Staphylococcus aureus using agar diffusion methods. Crude NCFS was obtained from seven isolates from pado by centrifuging their growth media, and the supernatant was neutralized. The proteinaceous nature of crude NCFS was confirmed after artificial digestion with proteolytic pepsin. Ultrafiltration was used to partially purify crude NCFS. Antibacterial peptides were further analyzed using liquid chromatography-high resolution mass spectrometry (LC-HRMS). All NCFS showed antibacterial activity against S. typhimurium, but only three isolates showed apparent inhibitory activity against S. aureus. The Lactiplantibacillus plantarum B3Iw4 isolate was selected for further study. The low-molecular-weight NCFS fraction (in the range of 317.20–1,494.70688 Da) after ultrafiltration by a 3 kDa membrane exhibited a threefold increase in specific activity against S. aureus compared to the crude NCFS. LC-HRMS analysis identified 20 peptides in the partially purified NCFS. The peptide, AAERAGAAALAMHGR, was predicted to have antibacterial activity after matching with the BIOPEP-UWM database, with a matched sequence AA. This peptide has relatively high hydrophobicity and is interesting for further study.
Co-Authors Abdul Choliq Abdul Munif Agustina, Lenny Aida Fadhilah Anastasia Fitria Devi Annisa Defriana Anuraga Jayanegara Anuraga Jayanegara Arum Safriana Dewi Badrut Tamam Betty S. L. Jenie Budi Nurtama C Hanny Wijaya Chalisya, Nadiah Cony Arisya Putri Dahrul Syah Dede Robiatul Adawiyah Dedi Fardiaz Dian Herawati Dian Muzdalifah Diana Ayu Nindita Dias Indrasti Dina Mariana Dina Mariana Dodik Briawan Dwi Rahmawati Dwi Rahmawati Dwiyitno Dwiyitno Dwiyitno Dwiyitno Elis Nina Herliyana Eni Kusumaningtyas Faiza A Dali farida ariyani Farida Ariyani Feri Kusnandar Hari Eko Irianto Hari Eko Irianto Harsi D. Kusumaningrum Irma Isnafia Arief Istiqamah Istiqamah Jan Schwarzbauer Jan Schwarzbauer Kurnianto, Muhammad Alfid Laras Cempaka Larissa Dsikowitzky Larissa Dsikowitzky Leonie Margaretha Widya Pangestika, Leonie Margaretha Widya Lilis Nuraida Madaniyah, Siti Maggy T. Suhartono Maggy T. Suhartono Maggy Thenawidjaja Suhartono Masao Goto Maya Saputri Melzer, Guido Mira Febrianti Muhammad Anwari Sugiharto Muhammad Hanafi N. Nurjanah Nancy Dewi Yuliana Neny Mariyani Ni Wayan Triwulandhari Nina Artanti Noer Laily Nuri Andarwulan Palupi, Nurheni Sri Puspo Edi Giriwono Rahmatia Garwan Ririn Anggraeni Rizaludin Nur Rizki Maryam Astuti Santi Ambarwati Sinta Simatupang Siti Madaniyah Sobir Sobir Sri Sugiwati Sugoi Marsaputra Karsodimejo Suliantari Suliantari SULISTIANI SULISTIANI Suyanto Suyanto TATI NURHAYATI Tatik Khusniati Taufik, Moh Tika Setianingrum Tuti Rostianti Maulani Warsono El Kiyat Weinreich, Bernd Widya Eka Prayitno Widya Puspantari Winiati P. Rahayu Winiati P. Rahayu Wisnu Ananta Kusuma Wisnu Broto Yane Regiyana Yeni Restiani Yuko Takano Ishikawa Yuli Maulidiyah Yulina Lailatul Maslukhah Zatil Afrah Athaillah Zulaikhah Zulaikhah Zulaikhah Zulaikhah, Zulaikhah