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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan Jurnal Silvikultur Tropika (JST) Jurnal Pengolahan Hasil Perikanan Indonesia VALENSI JURNAL KIMIA SAINS DAN APLIKASI Agrointek Jurnal Penelitian Pascapanen Pertanian Jurnal Teknologi Pertanian Jurnal Kimia dan Kemasan agriTECH Indonesian Journal of Biotechnology Warta IHP (Warta Industri Hasil Pertanian) Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Jurnal Kimia Terapan Indonesia Indonesian Food and Nutrition Progress JURNAL SELULOSA Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Jurnal Mutu Pangan : Indonesian Journal of Food Quality Jurnal Kimia dan Kemasan Policy Brief Pertanian, Kelautan, dan Biosains Tropika Jurnal Standardisasi
Hanifah Nuryani Lioe
Departemen Ilmu Dan Teknologi Pangan, Fakultas Teknologi Pertanian, IPB University, Bogor, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Public Health Social Sciences Other

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Isoflavones and Bioactivities in Over-fermented Tempeh Extracts Anastasia Fitria Devi; Dian Muzdalifah; Zatil Afrah Athaillah; Hanifah Nuryani Lioe; Nina Artanti
Jurnal Kimia Sains dan Aplikasi Vol 24, No 7 (2021): Volume 24 Issue 7 Year 2021
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (435.839 KB) | DOI: 10.14710/jksa.24.7.244-251

Abstract

Tempeh is nutritious food prepared through solid-state fermentation of cooked and dehulled soybeans with Rhizopus sp. for about 48 h. Fermentation beyond 48 h resulted in over-fermented tempeh. There may or may not have been similar research done before, especially related to its antioxidant and cytotoxicity. This study aims to determine the characteristics of fermented tempeh for up to 156 h. Samples were fermented at 0, 24, 48, 60, 72, 84, 96, 108, 120, and 156 h. Samples were dried, grounded, and extracted with acetone, followed by defatting with n‑hexane. Extracts were dissolved in organic solvents for free radical scavenging activity (FRSA) and cytotoxicity assays. The 120-h tempeh extract, at the concentration of 1,000 μg/mL, demonstrated the highest FRSA (81.31% inhibition) against 100 µM 2,2-diphenyl-1-picrylhydrazyl (DPPH) solution. Meanwhile, the 108-h tempeh extract at 1,000 μg/mL possessed the highest cytotoxicity (IC50 of 2.54 μg/mL) against MCF-7 breast cancer cell lines. Liquid Chromatography-Mass Spectrometry/Mass Spectroscopy (LC-MS/MS) analysis revealed the presence of daidzin, genistin, daidzein, and genistein in all extracts. Extracts prepared from 108 h and 120 h tempeh stood out from other extracts in the Partial Least Square (PLS) bi-plot due to their high percentage of inhibition, low response of daidzin, and high responses of the other three isoflavones. The cytotoxicity assays of the standard isoflavones showed that genistein had the lowest IC50 value at 4.82 ± 0.11 μg/mL. Standard genistein showed a low percentage of inhibition at 29.79 ± 9.10.
Karakterisasi Fisiko-Kimia Biji dan Kulit Ari Kacang Bogor Asal Jampang-Sukabumi Jawa Barat Rizki Maryam Astuti; Nurheni Sri Palupi; Maggy Thenawidjaja Suhartono; Hanifah Nuryani Lioe; Eni Kusumaningtyas; Laras Cempaka
Jurnal Teknologi dan Industri Pangan Vol. 33 No. 2 (2022): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.6066/jtip.2022.33.2.178

Abstract

The traditional cultivation and limited use of bambara groundnut (Vigna subterranea) seed and coat have encouraged the development of this commodity. The aim of this research was to characterize the seed and coat of bambara groundnut from Jampang, Kab. Sukabumi, West Java. Analysis on bambara groundnut seed including proximate analysis, in vitro protein digestibility, starch content, and dietary fiber, as well as analysis on its coat including anthocyanin, total phenolic, antioxidant activity, phytic acid and tannin, were examined in this study. The results showed that bambara groundnut seed from Jampang-Sukabumi contained 16.53% proteins, 3.04% ash, 7.83% fats and 55.22% carbohydrates in dry basis (db). The carbohydrates consisted of starch 52.71% and dietary fiber 7.47% (db). The protein had an in vitro protein digestibility of 41.65% db. The purple seed coat contained of 1.51% anthocyanin, 25.85 mg/g total phenolic content (as gallic acid equivalent), antioxidant activity at 82.75% inhibition of free radical DPPH, 6.37 mg/g phytic acid, and 96.79 mg/g tannin (as tannic acid equivalent) in dry basis. The relatively high content of tannin and antioxidant activity but very low phytic acid content, make the bambara seed coat a potential source for tannin, meanwhile the bambara groundnut is potential as a nutrition source.
Validasi Metode Analisis Patulin pada Buah Pisang menggunakan Reversed-Phase HPLC-MWD Arum Safriana Dewi; Winiati P. Rahayu; Hanifah Nuryani Lioe
Warta Industri Hasil Pertanian Vol 40, No 1 (2023)
Publisher : Balai Besar Standardisasi dan Pelayanan Jasa Industri Agro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32765/wartaihp.v40i1.7733

Abstract

ABSTRAK: Senyawa kimia patulin merupakan mikotoksin yang banyak mengontaminasi buah-buahan dan menimbulkan dampak negatif bagi kesehatan. Data kontaminasi patulin pada buah apel telah banyak dilaporkan, sedangkan pada buah tropis, khususnya pisang, masih sulit ditemukan. Tujuan penelitian ini adalah memvalidasi metode analisis patulin agar diperoleh metode yang siap digunakan untuk analisis patulin pada pisang. Penelitian ini menggunakan HPLC-MWD pada panjang gelombang 276 nm dengan fase diam C18 dan fase gerak asetonitril: H2O (10:90 v/v), laju alir 0,8 mL/menit. Linieritas instrumen dan metode diperoleh dengan nilai R2 secara berurutan yaitu0,9939 dan 0,9960. Limit deteksi instrumen (LDI) dan limit deteksi metode (LDM), masing-masing adalah 10,42 dan 42,80 ng/g. Rata-rata % recovery yang diperoleh sebesar 91,99% dengan rata-rata repeatabilitas 5,84%. Pada sampel empat buah pisang yang diambil dari empat tempat penjualan di kota Bogor dan dianalisis dengan metode ini menunjukkan tidak terdeteksi patulin (<LDM) pada semua sampel. Penggunaan volume pelarut setengah kali volume metode standar terbukti dapat digunakan untuk analisis patulin dengan matriks sampel pisang.
Modulasi Kadar Pati Resisten Berbagai Pangan Karbohidrat melalui Pemanasan Microwave: Meta-Analisis Sugoi Marsaputra Karsodimejo; Feri Kusnandar; Hanifah Nuryani Lioe; Anuraga Jayanegara
Jurnal Teknologi dan Industri Pangan Vol. 34 No. 2 (2023): Jurnal Teknologi dan Industri Pangan
Publisher : Perhimpunan Ahli Teknologi Pangan Indonesia bekerjasama dengan Departemen Ilmu dan Teknologi Pangan, IPB University Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.6066/jtip.2023.34.2.210

Abstract

Starch is extensively utilized in food processing for various purposes. However, the use of native starch is limited due to its unsuitability with processing conditions or products characteristics. Physical modification of starch is commonly employed to enhance the properties of native starch. The physical starch modification using microwave heating is presently developed due to its more efficient energy consumption than that of traditional heating methods. The process of microwave heating followed by cooling has been found to induce the formation of type 3 resistant starch (RS3). However, the effects of microwaving heating towards the the increase of resistant starch contents varies among researchers. For this reason, a meta-analysis was conducted to evaluate the effect of microwave heating on the levels of resistant starch in carbohydrate sources such as cereals, pulses, and tubers. The objective of this study was to analyze the carbohydrate food groups that demonstrated the most significant increase in resistant starch levels due to microwave heating, and to determine the optimal microwave treatment parameters within these food groups using meta-analysis. The findings indicate that microwave heating treatment is particularly effective for cereals, with rice being the most responsive. The most favorable treatment parameters include a power range of 401-600 W, heating time of 60-99 s, and starch moisture content of 40-60%.
Extraction and Physicochemical Characterization of Pectin from Noni (Morinda citrifolia) Fruit Maulani, Tuti Rostianti; Lioe, Hanifah Nuryani; Arief, Irma Isnafia; Kusumaningrum, Harsi Dewantari
Jurnal Kimia Valensi Jurnal Kimia VALENSI, Volume 10, No. 1, May 2024
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v10i1.37702

Abstract

Noni (Morinda citrifolia) is a popular plant in Asian countries and has great potential as pectin source for thickening agents in food products. In general, ethanol has been widely used to precipitate pectin, but the proportion can affect the quality of the product. Therefore, this study aimed to characterize the physicochemical properties of pectin from noni fruit. Extraction was performed using 0.1 N HCl at pH 1.5 and heated at 80°C for 15 min, followed by precipitation with different proportions of 96% ethanol including A (1:1), B (1:2), and C (1:3) v/v, while commercial orange pectin was used as a reference. The results showed that all pectin extracted belonged to the high methoxyl category. The FTIR spectra confirmed the presence of important functional groups including OH-hydroxyl, CH3-saturated aliphatic methyl, C=O carbonyl, and -O- cyclic ether. Furthermore, the crystalline and amorphous structures of noni pectin were confirmed by X-ray diffraction observation. The highest yield was achieved using the proportion of 1:3, followed by 1:2, and 1:1 on a wet basis. The MeO and AUA content of sample B (1:2) were relatively similar to those of the reference. Based on extraction results, noni possesses good characteristics and has the potential for commercial pectin production.
Penciri Fisiko Kimia Umbi Bawang Merah Yang Unggul Sebagai Bahan Baku Bawang Goreng Wijaya, C. Hanny; Sobir, Sobir; Lioe, Hanifah Nuryani
Policy Brief Pertanian, Kelautan, dan Biosains Tropika Vol 4 No 2 (2022): Policy Brief Pertanian, Kelautan dan Biosains Tropika
Publisher : Direktorat Kajian Strategis dan Reputasi Akademik IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agro-maritim.0402.256-259

Abstract

Bawang merah goreng merupakan produk olahan bawang dengan pangsa pasar yang stabil. Saat ini belum ada kriteria mutu baku bawang goreng berdasarkan keinginan konsumen. Pemahaman mutu yang valid diperlukan dalam pengembangan produk terstandar, termasuk pemilihan bahan baku. Bawang goreng tanpa salut, berwarna coklat cerah, utuh, rasa gurih, aroma wangi khas bawang goreng, tidak tengik dan tidak pahit merupakan kriteria yang diinginkan oleh konsumen. Penelitian terhadap 6 varietas unggul bawang merah, yaitu Bima Brebes, Bauji, Super Philip, Rubaru, Tajuk dan Batu Ijo, menunjukkan bahwa penciri fisiko kimia umbi yang unggul sebagai bahan baku bawang merah goreng adalah bobot umbi besar, kadar karbohidrat sedang, kadar total asam amino sedang, dan kadar piruvat tinggi. Varietas Batu Ijo, disamping produktivitasnya tinggi juga menghasilkan bawang goreng dengan krtieria mutu unggul, dibanding 5 varietas lain, termasuk varietas Rubaru atau lebih dikenal sebagai varietas “Sumenep” yang selama ini dikenal sebagai bahan baku bawang goreng.
PENGEMBANGAN PROSES SAPONIFIKASI DAN KONDISI REVERSED PHASE-HPLC-MWD PADA ANALISIS KOLESTEROL TELUR DARI METODE STANDAR AOAC 994.10:2012 Didah Nur Faridah; Yulina Lailatul Maslukhah; Ririn Anggraeni; Hanifah Nuryani Lioe
JURNAL STANDARDISASI Vol 22, No 2 (2020)
Publisher : Badan Standardisasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31153/js.v22i2.795

Abstract

Penentuan komposisi fase gerak, efisiensi waktu analisis, kromatogram yang bagus dan kehandalan instrumen perlu dipertimbangkan sebelum pengembangan metode analisis kolesterol menggunakan Reversed Phase-HPLC-Multiwavelength Detector (MWD). Konsentrasi KOH dan waktu saponifikasi diketahui berpengaruh terhadap kadar kolesterol yang diperoleh. Tujuan penelitian ini yaitu untuk mengembangkan metode kondisi RP-HPLC-MWD sebelum digunakan untuk analisis kolesterol, menguji kehandalan instrumennya, serta mengembangkan metode analisis kolesterol dalam telur ayam broiler dengan mengkombinasikan konsentrasi KOH dan waktu saponifikasi, pada suhu 80°C. Kombinasi acetonitril:metanol sebagai fase gerak (80:20, 20:80, 10:90, 0:100 v/v) dengan laju alir 1.2 mL/menit dan fase gerak 0:100 dengan laju alir 1.0 dan 0.8 mL/menit pada pada λ= 200, 205, 210 nm. Konsentrasi KOH dan waktu saponifikasi yang digunakan untuk pengembangan metode analisis kolesterol telur ayam yaitu 5 dan 10 % selama 15, 30, 45 menit. Kondisi RP-HPLC-MWD yang terpilih yaitu komposisi fase gerak metanol 100%, dengan laju alir fase gerak 1.0 mL/menit, λ= 205 nm. Instrumen ini mempunyai linieritas yang bagus, R2 = 0.9991. Limit deteksi dan kuantifikasi instrumen ini secara berurutan yaitu 5 dan 10 μg/mL. Konsentrasi KOH sebesar 10% (b/v) dan waktu saponifikasi selama 15 menit menghasilkan kadar kolesterol tertinggi dengan presisi yang bagus. 
Characteristics of Pindang Boiling Water and Its Use to Produce Antioxidative Peptides through Trypsin Hydrolysis Dali, Faiza A.; Nurjanah, Nurjanah; Lioe, Hanifah N.; Suhartono, Maggy T.
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 19, No 3 (2024): December 2024
Publisher : :Agency for Marine and Fisheries Research and Human Resources, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.879

Abstract

Pemindangan is a traditional fish processing method in Indonesia, where fish are preserved by boiling in saltwater. This process results in a liquid as a by-product, which is often discarded without any further processing. The liquid contains a substantial amount of protein, reaching 1.04±0.03 mg/mL, which make it a potential source of bioactive peptides. This study aimed to produce bioactive peptides from pindang liquid which was hydrolysed by enzymes isolated from skipjack viscera. The hydrolysates were prepared by using extracted trypsin, which was also from a fish by-product. The trypsin-assisted hydrolysis was carried out at a temperature 60 °C and pH 8.0, while for the semi-purified trypsin, the process was run at 50 °C and pH 8.0. The hydrolysis time was operated at different periods of 15, 30, and 60 min. The results showed that the pindang liquid dominantly contained hydrophobic amino acids and the hydrolysate was still rich in protein content ranging from 0.56 to 0.65 mg/mL. Furthermore, hydrolysis time significantly influenced antioxidant activity of the peptide. The best antioxidant activity was shown at hydrolysis time of 15 min by crude extract enzymes, resulting in antioxidant activity of 0.90±0.01 mg ascorbic acid equivalent antioxidant capacity (AEAC)/mg protein and reducing power of 4.49±0.60 mg trolox equivalent antioxidant capacity (TEAC)/mg protein. Hydrolysis by using semi-purified trypsin showed antioxidant activity equivalent to 0.73±0.05 mg AEAC/mg protein and reducing power 6.54±0.34 mg TEAC/mg protein. Overall, the results revealed the conversion of pindang liquid into bioactive peptides showing antioxidant activity, and therefore, they might be potentially developed as functional foods.
Studi Senyawa Perisa pada Online Marketplaces di Indonesia: Jenis Senyawa Perisa dan Legalitasnya Agustina, Lenny; Lioe, Hanifah Nuryani; Andarwulan, Nuri
Jurnal Teknologi dan Industri Pangan Vol. 35 No. 1 (2024): Jurnal Teknologi dan Industri Pangan
Publisher : Perhimpunan Ahli Teknologi Pangan Indonesia bekerjasama dengan Departemen Ilmu dan Teknologi Pangan, IPB University Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.6066/jtip.2024.35.1.27

Abstract

Various flavoring agents purchased by micro small medium enterprises (MSME) could be obtained through online marketplace. Their types and legalities have never been investigated in Indonesia. The objective of this study was to obtain an overview of the availability of the flavoring agents on online marketplace and their legality. The legality is defined as the flavoring agents which are marketed and have its compliance to regulation of Indonesian Food and Drug Agency (BPOM), otherwise is illegal. The profiles of online marketed flavoring agents on marketplace are obtained through online survey of flavorings advertisements documented using a check sheet as the tool. Tokopedia and Shopee were chosen as samples in this study because they both are the most popularly e-commerce sites clicked by consumers. A non-probability sampling and sample screening were carried out to obtain eligible samples until it achieved a predetermined amounts. There were 399 flavoring agents observed, with result of 262 legal flavoring agents (65.7%) and the rests were illegal. Mostly, they were of domestic flavorings (98.5 and 88% respectively). The cases of non-conformities forward the rules were: advertising without packaging, repacking, unregistered brands, and incompatible information in their packaging and/or labels when it was cross-checked with BPOM data. Due to the high number of illegal flavoring agents sold online, monitoring of the online flavoring markets should become a priority program of Indonesian government, while consumers need to be cautious. The traders and producer have an urgent action in taking a moral responsibility for the legality of the flavoring agents being marketed.
Kajian meta-analisis pengaruh metode penyembelihan terhadap kualitas daging ruminansia Yuli Maulidiyah; Feri Kusnandar; Hanifah Nuryani Lioe; Anuraga Jayanegara
AGROINTEK Vol 19, No 2 (2025)
Publisher : Agroindustrial Technology, University of Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrointek.v19i2.25242

Abstract

Meats of consumable livestock for humans are commonly from ruminants, such as cows, goats, lambs, buffaloes, etc. The method of slaughtering the livestock at the slaughterhouse mostly applies non-stunning or stunning methods. The stunning method uses electricity, mechanics, and gas. The slaughtering methods influence the meat quality and results. Thus, further meta-analysis is important to provide valid and informative conclusions based on comprehensive investigations about the slaughtering method's effect on ruminant meat quality. The researchers applied the PRISMA diagram and the size of the Standard Mean Difference effect, SMD, with Hedge's formulation as the quantitative synthetical meta-analysis method. The results found that the slaughtering method with stunning and non-stunning methods did not significantly influence some parameters, such as pH, colors (L*, a*, and b*), the water holding capacity, cooking loss, and shear force. However, the methods significantly influenced the drip loss and myofibril fragmentation index parameters with SMD values 0.2, p-values 0.001 to 0.777, and heterogeneity values of 0% to 82.98%.
Co-Authors Abdul Choliq Abdul Munif Agustina, Lenny Aida Fadhilah Anastasia Fitria Devi Annisa Defriana Anuraga Jayanegara Anuraga Jayanegara Arum Safriana Dewi Badrut Tamam Betty S. L. Jenie Budi Nurtama C Hanny Wijaya Chalisya, Nadiah Cony Arisya Putri Dahrul Syah Dede Robiatul Adawiyah Dedi Fardiaz Dian Herawati Dian Muzdalifah Diana Ayu Nindita Dias Indrasti Dina Mariana Dina Mariana Dodik Briawan Dwi Rahmawati Dwi Rahmawati Dwiyitno Dwiyitno Dwiyitno Dwiyitno Elis Nina Herliyana Eni Kusumaningtyas Faiza A Dali farida ariyani Farida Ariyani Feri Kusnandar Hari Eko Irianto Hari Eko Irianto Harsi D. Kusumaningrum Irma Isnafia Arief Istiqamah Istiqamah Jan Schwarzbauer Jan Schwarzbauer Kurnianto, Muhammad Alfid Laras Cempaka Larissa Dsikowitzky Larissa Dsikowitzky Leonie Margaretha Widya Pangestika, Leonie Margaretha Widya Lilis Nuraida Madaniyah, Siti Maggy T. Suhartono Maggy T. Suhartono Maggy Thenawidjaja Suhartono Masao Goto Maya Saputri Melzer, Guido Mira Febrianti Muhammad Anwari Sugiharto Muhammad Hanafi N. Nurjanah Nancy Dewi Yuliana Neny Mariyani Ni Wayan Triwulandhari Nina Artanti Noer Laily Nuri Andarwulan Palupi, Nurheni Sri Puspo Edi Giriwono Rahmatia Garwan Ririn Anggraeni Rizaludin Nur Rizki Maryam Astuti Santi Ambarwati Sinta Simatupang Siti Madaniyah Sobir Sobir Sri Sugiwati Sugoi Marsaputra Karsodimejo Suliantari Suliantari SULISTIANI SULISTIANI Suyanto Suyanto TATI NURHAYATI Tatik Khusniati Taufik, Moh Tika Setianingrum Tuti Rostianti Maulani Warsono El Kiyat Weinreich, Bernd Widya Eka Prayitno Widya Puspantari Winiati P. Rahayu Winiati P. Rahayu Wisnu Ananta Kusuma Wisnu Broto Yane Regiyana Yeni Restiani Yuko Takano Ishikawa Yuli Maulidiyah Yulina Lailatul Maslukhah Zatil Afrah Athaillah Zulaikhah Zulaikhah Zulaikhah Zulaikhah, Zulaikhah