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Hubungan Parameter Kualitas Air dengan Sintasan dan Pertumbuhan Ikan Nilem (Osteochilus vittatus) Fatimatus Sa'adah; Ratna Djuniwati Lisminingsih; Husain Latuconsina
Jurnal Riset Perikanan dan Kelautan Vol 5 No 1 (2023): Jurnal Riset Perikanan dan Kelautan (JRPK)
Publisher : Universitas Muhammadiyah Sorong

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33506/jrpk.v5i1.2136

Abstract

Ikaln Nilem (Osteochilus vittatus) merupalkaln salah saltu jenis ikan konsumsi yang dalam beberapa tahun terakhir ini dikategorikan sebagai jenis ikan budidaya karena diharapkan menjadi komoditas untuk program ketahanan pangaln nasional. Penelitian ini bertujuan untuk mengetahui hubungan antara parameter kulaitas air dengan sintasan dan pertumbuhaln Ikan Nilem pada media dengan pemberian pakan yang berbeda. Metode yalng digunalkaln adalah metode eksperimen. Hasil penelitian menunjukkan bahwa terdapat hubungan antara parameter lingkungan dengan sintasan dan pertumbuhan Ikan Nilem, dimana kuallitals air masih dalam kisaran optimum baik pH, suhu dan DO sehingga mampu menunjang sintasan dan pertumbuhan ikan dengan baik. pH memiliki hubungan negatif yang sangat kuat dengan sintasan, pertumbuhan panjang dan bobot benih Ikan Nilem, sedangkan nilai suhu memilki hubungan negatif lemah terhadap sintasan, pertumbuhan panjang dan bobot. Sebaliknya nilai oksigen terlarut memiliki hubungan positif kuat dengan sintasan, dan sangat kuat dengan pertumbuhan panjang dan bobot Ikan Nilem.
Pengaruh Kualitas Air Terhadap Daya Tetas Telur Ikan Nila (Oreochromis niloticus) Ditambah Ekstrak Daun Pepaya (Carica papaya) dengan Dosis Berbeda Afifa Muning Saputry; Husain Latuconsina; Ratna Djuniwati Lisminingsih
Jurnal Riset Perikanan dan Kelautan Vol 5 No 2 (2023): Jurnal Riset Perikanan dan Kelautan (JRPK)
Publisher : Universitas Muhammadiyah Sorong

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Nile tilapia (Oreochromis niloticus) is a freshwater fishery commodity that has commercial value. Very fast growth and has a distinctive meat taste make tilapia so popular with people both in Indonesia and abroad. However, a decrease in water quality can affect growth, including the hatchability of tilapia eggs. This study aims to determine the effect of water quality given papaya leaf extract (Carica papaya) with different doses on the hatchability of nile tilapia (Oreochromis niloticus) eggs. The method used is the experimental method. Data were analyzed using multiple linear regression to determine the effect of water quality on the hatchability of Nile tilapia eggs. The research results obtained were temperatures ranging from 25.1 to 27.50C, for pH ranging from 6.1 to 8.53, and for DO ranging from 5.16 to 6.70 and still within optimal limits for the hatching and growth of Nile tilapia eggs (Oreochromis niloticus). The research results found that temperature and pH had a small effect on the hatchability of Nile tilapia eggs, respectively 16.79% and 16.44%, while dissolved oxygen had a large effect on the hatchability of fish eggs, a value of 86.21. %.
The effect of papaya leaf extract (Carica papaya) with concentration on egg hatchability of nile tilapia (Oreochromis niloticus) Afifa Muning Saputry; Husain Latuconsina; Ratna Djuniwati Lisminingsih
Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Vol 7, No 2 (2023)
Publisher : Sangia Research Media and Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29239/j.akuatikisle.7.2.113-116

Abstract

Delays in hatching of fish eggs are influenced by several factors such as water quality instability during rearing, including; temperature, DO, pH. Papaya leaf is one of the plants that can be used to accelerate the hatchability of fish eggs. Papaya itself can be used as a component to boost the immune system because it contains the enzyme papain. The purpose of this study was to compare the hatchability of nile tilapia (Oreochromis niloticus) eggs in the treatment of papaya leaf extract (Carica papaya) with different doses. This research was conducted at the Punten Cultivation Fisheries Installation, Batu City in December 2022. Using a Completely Randomized Design (CRD) where there were 4 treatments, namely: A) papaya leaf extract 4 mL/L, B) papaya leaf extract 6 mL/L, C ) papaya leaf extract 10 mL/L, and D) control without treatment. In each treatment there were 3 repetitions. The ANOVA test was used to compare the effect of papaya leaf extract on hatchability of tilapia eggs, and was followed up with the BNT test if there were significant differences in the treatments. The results showed that there were significant differences in the treatment with the addition of papaya leaf extract on the hatchability of tilapia eggs, with 4 mL/L treatment) being the best treatment in increasing the hatchability percentage of tilapia eggs by 88.33%. There is a tendency that the higher the papaya leaf extract, the egg hatchability decreases.
Hatchability of Eggs and Larvae Survival of Common Carp (Cyprinus Carpio) Punten Strain On Exposure to Endosulfan Insecticide with Different Concentrations Karim, Ghomamul; Latuconsina, Husain; Lisminingsih, Ratna Djuniwati
Agrikan Jurnal Agribisnis Perikanan Vol. 15 No. 2 (2022): Agrikan: Jurnal Agribisnis Perikanan
Publisher : Fakultas Pertanian, Universitas Muhammadiyah Maluku Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52046/agrikan.v15i2.1152

Abstract

One type of organochlorine pesticide used by farmers is endosulfan. Excessive use of pesticides can cause environmental and water pollution and have an impact on the life of freshwater biota such as goldfish (Cyprinus carpio) Punten strain. The purpose of this study was to determine the value of LC50-168 hours of endosulfan insecticide on mortality of carp (Cyprinus carpio) eggs and to compare egg hatchability and survival of goldfish (Cyprinus carpio) larvae on exposure to endosulfan insecticides with different concentrations. This study used a completely randomized design (CRD) with 3 treatments and 1 control. Each treatment was repeated three times, namely P0 = 0 ppm (control), P1 (0.01 ppm), P2 (0.1 ppm), and P3 (1 ppm). Analysis of Variens (ANOVA) and DNMRT (Duncan New Multiple Range Test) were used to compare egg hatchability and larval survival at different concentrations of endosulfan insecticides. The results of the acute toxicity test (LC50-168 hours) of carp eggs were at a concentration of 0.6 ppm, there was a significant difference in hatchability of goldfish eggs (Cyprinus carpio) Punten strain exposed to endosulfan insecticide exposure with different concentration levels the higher the endosulfan concentration. used, the lower the hatchability of goldfish (Cyprinus carpio) eggs, and the survival rate of goldfish (Cyprinus carpio) larvae was different at various levels of endosulfan concentration. The higher the endosulfan concentration, the lower the survival rate of goldfish (Cyprinus carpio) larvae.
Egg Hatching and Larval Survival of Common Carp (Cyprinus carpio Linnaeus, 1758) Againts Exposure to Carbamate Insecticides with Different Concentrations Ula, Linda Zahrotul; Latuconsina, Husain; Lisminingsih, Ratna Djuniwati
Agrikan Jurnal Agribisnis Perikanan Vol. 16 No. 1 (2023): Agrikan: Jurnal Agribisnis Perikanan
Publisher : Fakultas Pertanian, Universitas Muhammadiyah Maluku Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52046/agrikan.v16i1.1187

Abstract

The purpose of this study was to determine the value of Hours from the use of carbamate insecticides on hatchability of Common carp (Cyprinus carpio) eggs, to compare hatchability of carp (Cyprinus carpio) eggs exposed to carbamate insecticides at different concentration levels, to compare survival rates. goldfish exposed to carbamate insecticides at different concentration levels. This study used a completely randomized design (RAL) where there were 3 treatments and 1 control. In each treatment there were 3 replications. Data analysis used ANOVA and DNMRT tests. The results showed that the average percentage of goldfish egg hatchability was at a concentration of 0.4 ppm with 58% hatchability. While the percentage of survival is at a concentration of 0.4 ppm with hatchability of eggs is 56%. The results of acute toxicity test Hours of carp eggs are at a concentration of 0.8 ppm. The conclusion obtained is that the acute concentration of hours of carbamate pesticides with the active ingredient karbosulfan in Common carp eggs (Cyprinus carpio) is a concentration of 0.8 ppm. There are differences in egg hatchability and survival rate of carbamate (Cyprinus carpio) larvae on exposure to carbamate insecticides with different concentrations, and there is a tendency that the higher the concentration of carbamate insecticides used, the lower the hatchability of eggs and carbamate (Cyprinus carpio) larvae survival.
The effect of papaya leaf extract (Carica papaya) with concentration on egg hatchability of nile tilapia (Oreochromis niloticus) Saputry, Afifa Muning; Latuconsina, Husain; Lisminingsih, Ratna Djuniwati
Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Vol 7, No 2 (2023)
Publisher : Sangia Research Media and Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29239/j.akuatikisle.7.2.113-116

Abstract

Delays in hatching of fish eggs are influenced by several factors such as water quality instability during rearing, including; temperature, DO, pH. Papaya leaf is one of the plants that can be used to accelerate the hatchability of fish eggs. Papaya itself can be used as a component to boost the immune system because it contains the enzyme papain. The purpose of this study was to compare the hatchability of nile tilapia (Oreochromis niloticus) eggs in the treatment of papaya leaf extract (Carica papaya) with different doses. This research was conducted at the Punten Cultivation Fisheries Installation, Batu City in December 2022. Using a Completely Randomized Design (CRD) where there were 4 treatments, namely: A) papaya leaf extract 4 mL/L, B) papaya leaf extract 6 mL/L, C ) papaya leaf extract 10 mL/L, and D) control without treatment. In each treatment there were 3 repetitions. The ANOVA test was used to compare the effect of papaya leaf extract on hatchability of tilapia eggs, and was followed up with the BNT test if there were significant differences in the treatments. The results showed that there were significant differences in the treatment with the addition of papaya leaf extract on the hatchability of tilapia eggs, with 4 mL/L treatment) being the best treatment in increasing the hatchability percentage of tilapia eggs by 88.33%. There is a tendency that the higher the papaya leaf extract, the egg hatchability decreases.
analisis kadar protein dan vitamin c pada sambal-ikan sebelum dan sesudah diolah Aisyi, Dinda Rohadatul; Santoso, Hari; Lisminingsih, Ratna Djuniwati
Jurnal SAINS ALAMI (Known Nature) Vol. 2 No. 1 (2019)
Publisher : FMIPA UNISMA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/j.sa.v2i1.2957

Abstract

Fish-Chili- sauce processed by a mixture of cayenne pepper (Capsicum frutescens), tomatoes (Lycopersicum esculentum) and Deles fly fish (Decapterus macrosoma) as fast-food sources of protein that is favored by the public. The purpose of this study are to determine the protein and vitamin C levels of fish-chili-sauce before and after processing. The research method are used completely randomized design with two treatments, namely treatment 1 measured levels of protein and vitamin C before processing; treatment 2 measures the levels of protein and vitamin C after processing. The protein test was performed using the Kjeldahl method with a Spectrophotometer Technique. Vitamin C testing uses the Iodimetry method. The results showed that protein levels of fish before processing = 19.31% and = 20.24 mg/100g of vitamin C. After processing the fish protein content  = 15.63% and = 16.28 mg/100g of vitamin C levels. Decreased fish protein levels and vitamin C levels were thought to be due to the length of processing time. The results of the analysis of the T-Test Pairs showed that there are no significant difference in the protein content of fish-chili-sauce before and after processing, but it tended to decrease. The results of the analysis of the Pairs T-Test showed that there are significant differences in the levels of vitamin C of fish-chili-sauce before and after processing.ABSTRAKSambal-ikan hasil olahan campuran cabai rawit (Capsicum frutescens), tomat (Lycopersicum esculentum) dan ikan layang Deles (Decapterus macrosoma) sebagai sumber protein cepat saji yang digemari oleh masyarakat. Tujuan penelitian ini untuk mengetahui kadar protein dan vitamin C sambal-ikan sebelum dan sesudah diolah. Metode penelitian di gunakan Rancangan Acak Lengkap dengan 2 perlakuan yaitu perlakuan 1 mengukur kadar protein dan vitamin C sebelum diolah; perlakuan 2 kadar protein dan vitamin C sesudah diolah. Uji protein dilakukan menggunakan metode Kjeldahl dengan Teknik Spektrofotometer. Uji vitamin C menggunakan metode Iodimetri. Hasil penelitian menunjukkan kadar protein 19,31% dan kadar vitamin C 20,24 mg/100g. 15,63%. dan kadar vitamin C16,28 mg/100g. Penurunan kadar protein ikan dan kadar vitamin C diduga karena lamanya waktu proses pengolahan. Hasil analisi uji T-Test Pairs menunjukkan tidak ada perbedaan yang signifikan pada kadar protein sambal-ikan sebelum dan sesudah diolah, namun cenderung mengalami penurunan. Hasil analisis uji T-Test Pairs menunjukkan ada perbedaan yang signifikan pada kadar vitamin C sambal-ikan sebelum dan sesudah diolah.
Uji Limbah Hasil Fermentasi Buah Maja (Aegle marmelos) Terhadap Pertumbuhan Tanaman Sawi Pakcoy (Brassica rapa l.) Safitri, Silvia Eka; Laili, Saimul; Lisminingsih, Ratna Djuniwati
Jurnal SAINS ALAMI (Known Nature) Vol. 4 No. 1 (2021)
Publisher : FMIPA UNISMA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/j.sa.v4i1.8853

Abstract

The Maja plant is a plant of the Rutaceae family that from tropical and subtropical regions of America. The benefits of Maja fruit fermentation waste can be used as organic liquid fertilizer. Pakcoy mustard is a vegetable that is needed by humans to meet their daily needs. The need for the vegetable market, especially Pakcoy mustard, has increased from year to year. Fertilizing plants is redundant to replace nutrients transported by plants, especially if the soil used for plant cultivation has a low fertility level, one of which is by utilizing the fermented Maja fruit waste can be used as liquid organic fertilizer containing elements macro and micronutrients needed by plants. This study aims to determine the effect of giving Maja fruit fermentation waste to the growth of mustard Pakcoy and to determine the best concentration for the growth of mustard Pakcoy. The method used in this study was an experimental method using a one-factor completely randomized design study (CRD) with concentrations of 0%, 10%, 20%, 30%, and 40% respectively 4 times with 5 treatments. The parameters observed in this study included plant height, number of leaves, root length, wet weight, dry weight, and abiotic factors. The research data were analyzed using analysis of variance (ANOVA), then if there was a significant difference, the LSD test was continued at 5%. The results showed that giving Maja fruit fermentation waste with a concentration of 40% affected increasing the wet weight of Pakcoy mustard with an average value of 82.50 grams.Keywords: Pakcoy Mustard, Maja Fruit Fermented Waste, Organic FertilizerABSTRAKTanaman maja adalah tumbuhan dari famili Rutaceae berasal dari daerah tropis dan subtropis di Amerika. Manfaat limbah fermentasi buah maja dapat digunakan sebagai pupuk cair organik. Sawi Pakcoy adalah sayuran yang sering diolah oleh manusia untuk memenuhi kebutuhan pangan. Terjadi peningkatan kebutuhan pakcoy setiap periode tahun. Pemberian pupuk terhadap tanaman mutlak diperlukan untuk menggantikan unsur hara. Media tanam budidaya sawi pakcoy pada tanah dengan tingkat kesuburan rendah perlu pemupukan, salah satu alternatif dengan pupuk cair organik limbah fermentasi buah maja yang mudah diperoleh. Limbah tersebut terbukti mengandung unsur hara makro dan mikro yang dibutuhkan tanaman. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian limbah hasil fermentasi buah maja terhadap pertumbuhan tanaman sawi pakcoy pada konsentrasi yang paling baik bagi pertumbuhan. Metode penelitian dilakukan secara eksperimen menggunakan desain rancangan acak lengkap (RAL).Perlakuan konsentrasi dimulai dari 0%; 10%; 20%; 30%; 40% dengan 4 kali ulangan. Pengamatan yang dilakukan pada penelitian ini diantaranya tinggi tanaman, jumlah daun, panjang akar, bobot basah, bobot kering, dan faktor abiotik. Analisis anova digunakan dalam penelitian ini untuk mengetahui adanya perbedaan yang nyata pada setiap perlakuan kemudian dilanjut uji BNT 5%. Penelitian ini memberikan hasil bahwa pemberian limbah hasil fermentasi buah maja dengan konsentrasi 40% berpengaruh dalam peningkatan bobot basah sawi pakcoy dengan nilai rata-rata 82,50 g.Kata kunci : Sawi Pakcoy, Limbah Hasil Fermentasi Buah Maja, Pupuk Organik
Uji Efektivitas Residu Tembakau Sebagai Bioinsektisida Hama Plutella Xylostella pada Tanaman Sawi (Brassica juncea L.) Hafiana, Rina Alfi; Laili, Saimul; Lisminingsih, Ratna Djuniwati
Jurnal SAINS ALAMI (Known Nature) Vol. 4 No. 1 (2021)
Publisher : FMIPA UNISMA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/j.sa.v4i1.8895

Abstract

The mustard plant (Brassica juncea L) has been widely cultivated by local communities. The problem that occurs in mustard cultivation farmers is the attack of plant pests which can reduce the quality and quantity of mustard plants. The abundant amount of cigarette butts waste has the potential to be used as a source of insecticides. The nicotine in cigarette butts is acknowledge to be a potential nerve poison and is used as a raw material for various types of insecticides and the essential oil content in clove also contains eugenol which is a component that can be used as an inhibitor of bacterial and fungal proliferation and plays an effective role in controlling pests, can cause skin irritation, and other problems that will become contact poison for insect pests. One of the leaf destroying leaf plants is Plutella xylostella. This study aims to distinguish the effect of kretek cigarette butts and determine the effective concentration on pest mortality. This research was carried out at Biology Laboratory, Faculty of Mathematics and Natural Sciences, Islamic University of Malang. The research method used an Randomized Block Design (RBD) experimental with 5 treatments and 4 replications. ANOVA test results show there are significant differences and the pest mortality tend to increase  after being treated. The most effective results are 25% solution concentrations with 6.75% mortality.  Keywords: Bioinsecticide, Cretek Cigarette Waste, Plutella xylostella Pest ABSTRAKTanaman sawi (Brassica juncea L) telah banyak dibudidayakan oleh masyarakat sekitar. Permasalahan yang terjadi pada petani budidaya sawi adalah serangan hama tanaman yang dapat menurunkan kualitas  dan kuantitas tanaman sawi. Limbah puntung rokok jumlahnya yang sangat melimpah berpotensi untuk dimanfatkan sebagai sumber insektisida. Nikotin yang ada di puntung rokok diyakini dapat menjadi racun syaraf yang potensial dan digunakan sebagai bahan baku berbagai jenis insektisida serta kandungan minyak atsiri pada cengkeh juga mengandung eugenol yang merupakan komponen yang dapat digunakan sebagai penghambat perkembangbiakan bakteri dan jamur serta berperan efektif dalam mengendalikan hama, dapat menimbulkan iritasi kulit dan masalah masalah lain yang akan menjadi racun kontak untuk serangga hama. Salah satu hama tanaman sawi perusak daun yaitu Plutella xylostella. Penelitian ini bertujuan untuk membedakan pengaruh limbah puntung rokok kretek dan mengetahui konsentrasi efektif terhadap mortalitas. Penelitian ini dilakukan dilaboratorium Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Islam Malang. Metode penelitian menggunakan eksperimental Rancangan Acak Kelompok (RAK) dengan 5 perlakuan dan 4 ulangan. Hasil uji statistik ANOVA menunjukkan terdapat perbedaan yang signifikan serta cenderung mengalami peningkatan mortalitas hama setelah diberi perlakuan. Hasil yang paling efektif yaitu dengan konsentrasi larutan 25% dengan mortalitas 6,75%.  Kata kunci : Bioinsektisida, Limbah Puntung Rokok Kretek, Hama Plutella xylostella
Uji Ekstrak Buah Maja (Aegle marmelos) Sebagai Antibakteri Pada Bakteri Escherichia coli Apriliani, Susi; Syauqi, Ahmad; Lisminingsih, Ratna Djuniwati
Jurnal SAINS ALAMI (Known Nature) Vol. 4 No. 1 (2021)
Publisher : FMIPA UNISMA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/j.sa.v4i1.9907

Abstract

Maja fruit (Aegle marmelos) is a fruit that contains substances such as balm oil, 2-furocoumarins-psoralen and marmelosin (C13H12O). Maja fruit, roots and leaves have antibiotic properties. Plants that contain chemicals in the maja fruit can potentially act as antibacterials that inhibit bacterial growth. The bacteria that cause diarrhea are Coliform, Escherichia coli, Salmonella enterica and Shigella which are bacteria that cause food poisoning or gastrointestinal disorders. Chemical content of maja fruit (Aegle marmelos), namely alkaloids, terpenoids, coumarin, phenylpropanoids, tannins, polysaccharides and flavonoids. This study aims to determine the maja fruit extract has an anti-bacterial effect on minimum bacterial inhibitory growth and to determine the concentration of maja fruit extract on the minimum inhibitory growth of Escherechia coli bacteria. In this study, using the experimental method completely randomized design (CRD). Using 4 treatments and 6 repetitions. By using EMB and BGLB media. The mean of observations on the inhibitory growth test of Escherichia coli bacteria at a concentration of 19.2 was 0.43, a concentration of 35.7 was 0.48, at a concentration of 37.5 was 1.703 and a control was 0.33. Anova test results showed that there was a significant difference, after being treated with a concentration of 0%, 19.2%, 35.7% and 37.5%, the most effective results were with a solution concentration of 37.5% with an average of 1.703 mm.Keywords: Antibacterial, Maja Frutt, Escherechia coli ABSTRAK Buah maja (Aegle marmelos) adalah buah yang mengandung substansi seperti minyak balsem, 2-furocoumarins-psoralen dan marmelosin (C13H12O). Buah, akar dan daun maja mempunyai sifat antibiotik. Tumbuhan yang memiliki kandungan kimia pada buah maja dapat berpotensi sebagai antibakteri yang menghambat pertumbuhan bakteri. Bakteri yang menimbulkan diare adalah Coliform, Escherichia coli, Salmonella enterica, Shigella yang merupakan bakteri penyebab keracunan makanan atau gangguan saluran cerna. Kandungan kimia dari ekstrak buah maja yaitu alkaloid, terpenoid, kumarin, pherilpropanoid, tannin, polisakarida dan flavonoid. Tujuan penelitian ini untuk mengetahui ekstrak buah maja memiliki pengsruh antibakteri terhadap pertumbuhan hambat minimal bakteri Escherichia coli dan menentukan konsentrasi ekstrak buah maja terhadap pertumbuhan hambat minimal bakteri Escherichia coli. Dalam penelitian ini menggunakan metode eksperimental Rancangan Acak Lengkap (RAL). Menggunakan 4 perlakuan dan 6 kali ulangan. Dengan menggunakan media EMB dan BGLB. Rerata pengamatan pada pertumbuhan uji daya hambat bakteri pada konsentrasi 19,2 % yaitu 0,43 mm, konsentrasi 35,7% yaitu 0,48 mm, konsentrasi 37,5% yaitu I,70 mm dan pada kontrol 0,33 mm. Hasil uji ANOVA menunjukkan terdapat perbedaan signifikan, setelah diberi perlakuan konsentrasi 0%,19,2%,35,7% dan 37,5% hasil yang paling efektif yaitu dengan konsentrasi larutan 37,5% dengan rata-rata 1,703 mm. Kata kunci : Antibakteri, Buah Maja, Escherechia coli