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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Fitopatologi Indonesia Jurnal Ilmu Pertanian Jurnal Natur Indonesia Jurnal Ilmu Pertanian KULTIVAR AGROEKOTEKNOLOGI JURNAL ILMU PERTANIAN Kultivasi jurnal Ilmu Pertanian (Agricultural Science) International Journal of Basic and Applied Science PERTANIAN TROPIK Makara Journal of Science
Luthfi Aziz Mahmud Siregar
Universitas Sumatera Utara
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Electrical & Electronics Engineering Medicine & Pharmacology Physics

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Journal : Makara Journal of Science

 1 
PENGARUH KASEIN HIDROLISAT DAN INTENSITAS CAHAYA TERHADAP PRODUKSI BIOMASSA DAN ALKALOID CANTHINONE DI DALAM KULTUR SUSPENSI SEL PASAK BUMI (Eurycoma longifolia Jack) Siregar, Luthfi Aziz Mahmud; Keng, Chan Chan; Lim, Boey Peng
Makara Journal of Science Vol. 14, No. 1
Publisher : UI Scholars Hub

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Abstract

Effects of Casein Hydrolisate and Light Intensity on Production of Biomass and Canthinone Alkaloid in Cell Suspension Cultures of Pasak Bumi (Eurycoma longifolia Jack). A study on the effect of several concentrations of casein hydrolysate and light intensity to produce biomass and canthinone alkaloid in cell suspension culture of Pasak Bumi (Eurycoma longifolia Jack) was conducted. The research was an experimental study with block random design non factorial. For study of casein hydrolysate was used 8 (eight) of concentration treatments, i.e. 0.00, 0.10, 0.25, 0.50, 0.75, 1.00, 2.00, dan 5.00% (w/v). Beside for study of different light intensity effect was used five of light intensity, i.e. 0, 190, 290, 585, and 1525 lux. The results showed that addition 0.1–2.0% (w/v) of casein hydrolysate into culture medium did not show the effect on production of cell biomass compare with without casein hydrolysate. Total of alkaloid produce increased two fold in the culture medium containing 0.1% casein hydrolysate. The medium with addition 5% casein hydrolysate significantly decrease of increased fresh weight, dry weight and total of alkaloid compare with medium without or additions of casein hydrolysate at 0.1–2.0% concentration. Besides, the modifications on light intensity (0–1525 lux) did not effect the production of cell biomass in culture medium, but the place of E. longifolia Jack cell suspension culture at light of 1525 lux was found have to stimulate increased the total of 9- methoxycanthin-6-one in cells.
Co-Authors Abd. Rasyid Syamsuri Abdul Rauf Agus Pernando Simanjuntak Asima napitupulu Asma Ul Husna Ayu Oshin Yap Sinaga Baehaqi Beatrix Blandina BOEY PENG LIM Boey Peng-Lim CHAN LAI KENG Chan Lai-Keng Chandri Lidya Putri Nasution Diana Sofia Hanafiah DWI NINGSIH SUSILOWATI Dyanne Gisella P. Tambak Elseria Siburian Emmy Harso Kardhinata Emmy Rosita Ermando, Relly Erni Royani Harahap Eva Sartini Bayu Eva Sartini Bayu Firdani, Citra Fristy Rebecca Hasianta Sitohang Hanipah Tanjung Hapsoh Hasmawi Hasyim2 Hasmawi Hasyim2 Heri Hidayat Hilda Mei Yeni Harahap Hot Setiado Hot Setiado Hot Setiado I.M. Revandi Damanik Imam, muhammad Ira Tiarma Sari Damanik Jonatan Ginting Julita Julita Keng, Chan Chan Khairunnisa Lubis Khairunnisa Lubis Lambok Turnip Larosa Harahap Lili Herawati Siregar Linda Tri Wira Astuti Lisa Lisa Mawarni Lisnawita Lita Adelia Lollie A. P. Putri Agustina P Putri Lollie Agustina P Putri Lollie Agustina P. Putri Lollie Agustina P. Putri Lollie Agustina P. Putri Lollie Agustina P. Putri Mahyuddin Dalimunte Marpaung, Agustina Erlinda Milani, Ayu Mona Faronika1 Mona Faronika1 Nazriah pratiwi Nella Angelina Simanjuntak Nina Unzila Angkat Nini Rahmawati nuraminah nasution Permata Sari Harahap Purba, Hans Samuel Putri Hasanah Jumroh Retno Diah Setiowati Revandy Iskandar Muda Damanik Riski Aulia Risma Damayanti Rosmayati Rosmayati Rosmayati Rosmayati Rosmayati Rosmayati Rozaliana Rozaliana Sihotang, Tambun Siti Aminah Sopa Putri Tanjung Tengku Sabrina Triningsih Triningsih Ulfa Ridha Andini Utri Patma1 Utri Patma1 Yoppi Operasisco Gabesius Yusuf Husni Yusuf Husni