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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Fitopatologi Indonesia Jurnal Ilmu Pertanian Jurnal Natur Indonesia Jurnal Ilmu Pertanian KULTIVAR AGROEKOTEKNOLOGI JURNAL ILMU PERTANIAN Kultivasi jurnal Ilmu Pertanian (Agricultural Science) International Journal of Basic and Applied Science PERTANIAN TROPIK Makara Journal of Science

Luthfi Aziz Mahmud Siregar

Universitas Sumatera Utara

Author-ID : 310019

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Electrical & Electronics Engineering Medicine & Pharmacology Physics

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Pengaruh Jenis Eksplan dan Komposisi Media terhadap Pembentukan Tunas Tanaman Karet (Hevea brasiliensis Muell. Arg.) Secara In Vitro Emmy Rosita; Luthfi Aziz Mahmud Siregar; Emmy Harso Kardhinata
Jurnal Agroekoteknologi Vol 4, No 1 (2016)
Publisher : Universitas Sumatera Utara

The aim of this research is to evaluate the influence of medium composition and types of explant on shoot formation of rubber tree (H. brasiliensis Muell. Arg.). The research was carried out in the In Vitro Culture Laboratory, PT. Perkebunan Nusantara III Kebun Gunung Pamela, Tebing Tinggi, Sumatera Utara, Indonesia. The research was conducted from March to July 2015. The research was arranged in completely randomized design with two factors, i.e.: types of explant and the medium with combination of growth regulatorsand fifteen replications. The results showed that interaction types of explant and medium with combination of growth regulators gave significant effect on percent of shoots.Key words: rubber, multiplication, explants, medium in vitroThe aim of this research is to evaluate the influence of medium composition and types of explant on shoot formation of rubber tree (H. brasiliensis Muell. Arg.). The research was carried out in the In Vitro Culture Laboratory, PT. Perkebunan Nusantara III Kebun Gunung Pamela, Tebing Tinggi, Sumatera Utara, Indonesia. The research was conducted from March to July 2015. The research was arranged in completely randomized design with two factors, i.e.: types of explant and the medium with combination of growth regulatorsand fifteen replications. The results showed that interaction types of explant and medium with combination of growth regulators gave significant effect on percent of shoots.Key words: rubber, multiplication, explants, medium in vitro

Karakteristik Pertumbuhan Vegetatif dari Beberapa Aksesi Tanaman Bangun-Bangun (Plectranthus amboinicus (Lour.) Spreng) nuraminah nasution; Luthfi Aziz M. Siregar; Eva Sartini Bayu
Jurnal Agroekoteknologi Vol 5, No 1 (2017)
Publisher : Universitas Sumatera Utara

Penelitian bertujuan untuk mengetahui karakteristik pertumbuhan vegetatif, kandungan sterol dan klorofil dari beberapa aksesi tanaman bangun-bangun. Penelitian dilaksanakan di rumah kasa Fakultas Pertanian, Universitas Sumatera Utara Medan, dimulai Desember 2015 sampai Februari 2016 menggunakan tanaman bangun-bangun asal Medan (Krakatau), Medan (Tuntungan), Sibolangit, Simalungun dan Brastagi. Hasil penelitian menunjukkan bahwa karakter-karakter morfologi pada aksesi tanaman asal Medan (Tuntungan), Sibolangit, Simalungun dan Brastagi tidak berbeda nyata, tetapi berbeda nyata dengan aksesi tanaman asal Medan (Krakatau) pada karakter warna batang dan warna permukaan daun bagian atas.

Pengaruh Jenis Eksplan dan Komposisi Zat Pengatur Tumbuh Terhadap Induksi Kalus Pada Tanaman Binahong (Anredera cordifolia (Ten.) Steenis) Ira Tiarma Sari Damanik; Rosmayati Rosmayati; Luthfi Aziz Mahmud Siregar
Jurnal Agroekoteknologi Vol 5, No 3 (2017)
Publisher : Universitas Sumatera Utara

Penelitian ini bertujuan untuk mengetahui pengaruh jenis eksplan dan komposisi zat pengatur tumbuh terhadap induksi kalus pada tanaman binahong (Anredera cordifolia (Ten.) Steenis). Penelitian ini dilaksanakan di Laboratorium Kultur Jaringan, Dinas Pertanian Sumatera Utara pada bulan Juli sampai dengan September 2016. Rancangan yang digunakan adalah rancangan acak lengkap dengan 2 faktor yaitu jenis eksplan (daun, batang, tangkai daun) dan zat pengatur tumbuh (MS; MS + 1 mg/L 2,4 D + 0,5 mg/L BAP; MS + 2 mg/L 2,4 D + 0,5 mg/L BAP; MS + 3 mg/L 2,4 D + 0,5 mg/L BAP; MS + 1 mg/L 2,4 D + 1 mg/L BAP; MS + 2 mg/L 2,4 D + 1 mg/L BAP; MS + 3 mg/L 2,4 D + 1 mg/L BAP). Hasil penelitian menunjukkan jenis eksplan berpengaruh nyata terhadap persentase terbentuk kalus. Komposisi zat pengatur tumbuh berpengaruh nyata terhadap persentase terbentuk kalus. Eksplan batang merupakan eksplan yang terbaik. Media MS + 3 mg/L 2,4 D + 0,5 mg/L BAP, MS + 2 mg/L 2,4 D + 1 mg/L BAP, dan MS + 3 mg/L 2,4 D + 1 mg/L BAP merupakan media yang terbaik untuk persentase terbentuk kalus.

The Potential Formation Of Embryogenic Callus Of The Hypoxia Condition Tolerant Of Soybean Varieties In Vitro Sopa Putri Tanjung; Revandy I. M. Damanik; Luthfi Aziz Mahmud Siregar
Jurnal Agroekoteknologi Vol 5, No 3 (2017)
Publisher : Universitas Sumatera Utara

The aims of the research was to determine the growth response and to identify the potential embriogenic callus of the hypoxia condition tolerant of soybean varieties in vitro. The research was conducted at the Plant Tissue Culture Laboratory and the Laboratory of Food Technology, Faculty of Agriculture, North Sumatera University, Medan, Indonesia, from March 2016 to September 2016. The result were analyzed with t-test with the combination between soybean varieties (Baluran, Gepak kuning, Willis) and the plant growth regulators (2,4-D, NAA, IAA) 10 mg/l. The parameters observed were: the percentage of plants forming callus, the callus colour, the callus texture, the callus histological, the fresh weight of callus, the total chlorophyll content, the protein concentration, the SOD activity and the POD activity. The result showed that the combination between varieties Gepak Kuning and Willlis with 10 mg/l 2,4-D significantly affected the growth of embriogenic callus, the fresh weight of callus, the total chlorophyll content, the protein concentration, the SOD and POD activities.

Pengaruh Zat Pengatur Tumbuh terhadap Induksi Akar (Rhizogenesis) pada Tanaman Bangun-Bangun (Plectranthus amboinicus (Lour.) Spreng) secara In Vitro Nella Angelina Simanjuntak; Luthfi Aziz Mahmud Siregar; Lollie Agustina P Putri
Jurnal Agroekoteknologi Vol 5, No 3 (2017)
Publisher : Universitas Sumatera Utara

Penelitian ini bertujuan untuk mengetahui pengaruh zat pengatur tumbuh terhadap induksi akar (rhizogenesis) pada P. amboinicus secara in vitro. Penelitian ini dilaksanakan di Laboratorium Kultur Jaringan UPT. Benih Induk Hortikultura Gedung Johor, Dinas Pertanian, Sumatera Utara, Indonesia. Penelitian ini dimulai pada bulan April 2016 sampai dengan Juli 2016. Rancangan yang digunakan adalah rancangan acak lengkap non faktorial dengan 7 perlakuan yaitu: MS + 1 mg/l NAA + 0,1 mg/l kinetin; MS + 2 mg/l NAA + 0,1 mg/l kinetin; MS + 3 mg/l NAA + 0,1 mg/l kinetin; MS + 1 mg/l 2,4 D + 0,1 mg/l kinetin; MS + 2 mg/l 2,4 D + 0,1 mg/l kinetin; MS + 3 mg/l 2,4 D + 0,1 mg/l kinetin; MS + 0,1 mg/l kinetin. Penelitian ini menggunakan 15 ulangan. Hasil penelitian menunjukkan bahwa perlakuan zat pengatur tumbuh memberikan pengaruh nyata terhadap berat segar akar dan jumlah akar tetapi belum memberikan pengaruh nyata terhadap persentase terbentuknya akar dan umur muncul akar. Medium MS + 3 mg/l NAA + 0,1 mg/l kinetin memberikan hasil terbaik pada persentase terbentuknya akar, berat segar akar, dan jumlah akar.

Eksplorasi dan Karakterisasi Morfologis Durian (Durio zibethinus Murr) di Kecamatan Tigalingga Kabupaten Dairi Sumatera Utara Nazriah pratiwi; Diana Sofia Hanafiah; Luthfi aziz mahmud siregar
Jurnal Agroekoteknologi Vol 6, No 2, April (2018)
Publisher : Universitas Sumatera Utara

Penelitian ini bertujuan untuk mengidentifikasi karakter morfologis Durian (Durio zibethinus Murr) di Kecamatan Tigalingga dan Pegagan Hilir Kabupaten Dairi Sumatera Utara.Penelitian ini dilakukan di Kecamatan Tigalingga dan Kecamatan Pegagan Hilir Kabupaten Dairi pada bulan Desember 2016 sampai dengan Januari 2017, data diidentifikasidengan metode survei deskriptif menggunakan panduan descriptors for durian(Bioversity Internasional)dengan teknik pengambilan sampel secara accidental sampling.Hasil penelitian menunjukkan bahwa di dua Kecamatan di Kabupaten Dairi terdapat 21 genotipe durian yang teridentifikasi. Hasil karakterisasi morfologis untuk bentuk tajuk (piramida, jorong, bulat, stengah membulat, lonjong dan tidak beraturan), permukaan batang (kasar), daun (empat persegi, bulat panjang dan bulat telur), buah (bulat ujung datar, bulat, bulat telur, lonjong, bulat panjang, dan ovoid), biji (lonjong jorong dan bulat). Kata kunci : karakter morfologi, durian, kabupaten Dairi

Selection to Stem Rot Disease Athelia rolfsii (Curzi)Resistant Level of SomeSoybean (Glycine max L. Merrill)Line and High Production on M5 Generation Mutia Rahmah; Diana Sofia Hanafiah; Luthfi Aziz Mahmud Siregar
Jurnal Agroekoteknologi Vol 6, No 4,Okt (2018)
Publisher : Universitas Sumatera Utara

This study aims to get individuals selected of some soybean Glycine max L. (Merrill) M5 generation with character resistant stem rot disease Athelia rolfsii (Curzi)and high production. This research was conducted in Plant Disease Laboratory and experimental field Faculty of Agriculture Universitas Sumatera Utara Medan, Indonesia. This research was conducted from December 2016 until July 2017. In this study using augmented design. The treatments were 15 mutant lines, the check variety were Anjasmoro, Agromulyo, and Kipas Putih varieties. The results showed that the appearance of agronomic characters observed in the inoculated medium of the fungus causes stem rot disease is lower than the optimum field without inoculation of fungus. Differences in the means values of the mutant line are in the larger stem diameter character of plant than with the inoculation of fungus. Broad sense heritability values were found in the number of pods per plant, number of seeds per plant, and seed weight per plant on the inoculated medium of the fungus and the optimum field. Selection performed on population M5resulted 7 individuals with high production character and 62 individuals with tolerance of stem rot disease character.

Identification of Morphological Characteristic of Dragon Fruit (Hylocereus sp.) in Sitinjo District of Dairi Regency North Sumatera Nina Unzila Angkat; Luthfi Azis Mahmud Siregar; Revandy Iskandar Damanik
Jurnal Agroekoteknologi Vol 6, No 4,Okt (2018)
Publisher : Universitas Sumatera Utara

This reserach aimed to identify the morphological chracteristics and phylogenetic relationship of Dragon Fruit (Hylocereus sp.) plants. The research was started from April 2017 until May 2017 at Sitinjo district of Dairi Regency North Sumatera with survey methode using dragon fruit descriptor of Hylocereus by International Union For The Protection Of New Varieties (UPOV). Sampling technique using purposive sampling. Exploration results was obtained 24 genotypes of Dragon Fruit plants based on identified fruit morphological characteristic. Based on clustering analyze the closest genetic relationship is on genotype G17 and G23 which has 2 differences of 7 characteristic with dissimilarity coeficient is 1,000 and the furthest genetic relationship is on genotype G18 and G26 with dissimilarity coeficien is 4,796 which has 5 differences of 7 characteristic.

Morphological Characteristics and Phylogenetic Relationship of Several Genotypes of Coconut (Cocos nucifera L.) in Silau Laut District Asahan Regency Risma Damayanti; Luthfi Aziz Mahmud Siregar; Diana Sofia Hanafiah
Jurnal Agroekoteknologi Vol 6, No 4,Okt (2018)
Publisher : Universitas Sumatera Utara

This research aimed to identify the morphological characteristics and phylogenetic relationship of several genotypes of coconut in Silau Laut district Asahan regency Sumatera Utara. The research was conducted from April to Mei 2017 by survey method based on coconut descriptor of International Plant Genetic Resources Institute (IPGRI) with sampling technique using accidental sampling. This result of research showed that in Silau Laut district there were 27 genotypes of coconut been identified. The result of morphologycal characteristics for crown shape (spherical, hemispherical, X-shaped ‘silhouette’), flower shape (normal and additional spathes or bracts), fruit shape (oblong, ovoid, angled, round), nut shape (pointed, ovoid, almost round, oblate). The closest phylogenetic relationship is G41 and G42 with coefficient dissimilarity 3,00 and farthest phylogenetic relationship is G40 and G45 with coefficient dissimilarity 12,53. The result of dendogam showed at scale 18 was formed 5 goups. Phylogenetic relationship of between genotypes of coconut in Silau Laut district Asahan regency still have a close level relationship.

Identifikasi Fenotipe Pisang Barangan (Musa acuminata Linn.) di Kabupaten Deli Sedang Sumatera Utara Beatrix Blandina; Luthfi Aziz Mahmud Siregar; Hot Setiado
Jurnal Agroekoteknologi Vol 7, No 1,Jan (2019)
Publisher : Universitas Sumatera Utara

Penelitian ini bertujuan untuk mengidentifikasi fenotipe tanaman pisang barangan (Musa acuminata Linn.) yang ada di Kabupaten Deli Serdang Sumatera Utara. Penelitian ini dilaksanakan di Kecamatan Sinembah Tanjung Muda Hilir Kabupaten Deli Serdang mulai bulan Maret sampai dengan Agustus 2017 menggunakan metode survei deskriptif dengan panduan descriptors for banana (International Plant Genetic Resources Institute) dengan teknik pengambilan sampel secara purposive sampling. Hasil penelitian ini menunjukkan bahwa hubungan kekerabatan terdekat ada pada sampel D1P1S1 dan D1P1S3 dengan nilai jarak terdekat senilai 0,000 dan hubungan kekerabatan terjauh pada sampel D1P3S3 dan D3P3S2 dengan nilai jarak terjauh senilai 5,000.

Co-Authors Abd. Rasyid Syamsuri Abdul Rauf Agus Pernando Simanjuntak Asima napitupulu Asma Ul Husna Ayu Oshin Yap Sinaga Baehaqi Beatrix Blandina BOEY PENG LIM Boey Peng-Lim CHAN LAI KENG Chan Lai-Keng Chandri Lidya Putri Nasution Diana Sofia Hanafiah DWI NINGSIH SUSILOWATI Dyanne Gisella P. Tambak Elseria Siburian Emmy Harso Kardhinata Emmy Rosita Ermando, Relly Erni Royani Harahap Eva Sartini Bayu Eva Sartini Bayu Firdani, Citra Fristy Rebecca Hasianta Sitohang Hanipah Tanjung Hapsoh Hasmawi Hasyim2 Hasmawi Hasyim2 Heri Hidayat Hilda Mei Yeni Harahap Hot Setiado Hot Setiado Hot Setiado I.M. Revandi Damanik Imam, muhammad Ira Tiarma Sari Damanik Jonatan Ginting Julita Julita Keng, Chan Chan Khairunnisa Lubis Khairunnisa Lubis Lambok Turnip Larosa Harahap Lili Herawati Siregar Linda Tri Wira Astuti Lisa Lisa Mawarni Lisnawita Lita Adelia Lollie A. P. Putri Agustina P Putri Lollie Agustina P Putri Lollie Agustina P. Putri Lollie Agustina P. Putri Lollie Agustina P. Putri Lollie Agustina P. Putri Mahyuddin Dalimunte Marpaung, Agustina Erlinda Milani, Ayu Mona Faronika1 Mona Faronika1 Nazriah pratiwi Nella Angelina Simanjuntak Nina Unzila Angkat Nini Rahmawati nuraminah nasution Permata Sari Harahap Purba, Hans Samuel Putri Hasanah Jumroh Retno Diah Setiowati Revandy Iskandar Muda Damanik Riski Aulia Risma Damayanti Rosmayati Rosmayati Rosmayati Rosmayati Rosmayati Rosmayati Rozaliana Rozaliana Sihotang, Tambun Siti Aminah Sopa Putri Tanjung Tengku Sabrina Triningsih Triningsih Ulfa Ridha Andini Utri Patma1 Utri Patma1 Yoppi Operasisco Gabesius Yusuf Husni Yusuf Husni

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