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All Journal Prosiding Seminar Nasional MIPA The Journal of Experimental Life Sciences (JELS) Media Penelitian dan Pengembangan Kesehatan Journal of Innovation and Applied Technology Research Journal of Life Science JFMR (Journal of Fisheries and Marine Research) Jurnal Penelitian Pendidikan IPA (JPPIPA) Riau Journal of Empowerment Samakia: Jurnal Ilmu Perikanan GANEC SWARA Journal of Aquaculture and Fish Health Jurnal Perikanan Pantura Jurnal Aquaculture Indonesia
MOHAMAD FADJAR
Fakultas Perikanan Dan Ilmu Kelautan, Universitas Brawijaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences Veterinary Other

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Journal : JFMR (Journal of Fisheries and Marine Research)

 1 
Uji Viabilitas Kandidat Vaksin Bakteri Aeromonas Hydrophila Menggunakan Metode Formalin-Killed dengan Konsentrasi Berbeda: Viability Assay of Aeromonas Hydrophila as Candidate Vaccine Using Formalin-Killed Method at Different Concentration Herlina, Cucun; Maftuch, Maftuch; Fadjar, Mohamad
JFMR (Journal of Fisheries and Marine Research) Vol. 9 No. 1 (2025): JFMR on March
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2025.009.01.7

Abstract

Salah satu upaya pencegahan yang dapat dilakukan untuk mengatasi penyakit pada ikan yaitu menumbuhkan kekebalan seperti vaksinasi karena kekebalan tubuh yang meningkat pada ikan dapat mencegah infeksi penyakit. upaya telah dilakukan untuk mengembangkan program imunisasi yang efektif dengan menggunakan vaksin yang dibuat dari sel utuh bakteri yang diinaktivasi menggunakan formalin dan/atau pemanasan Tujuan penelitian ini untuk mengetahui hasil uji viabilitas dan hasil uji adhesi sel epitel usus menggunakan vaksin inaktif formalin dengan konsentrasi berbeda. Metode dalam penelitian ini adalah metode eksperimen dengan rancangan percobaan menggunakan rancangan acak lengkap (RAL) terdiri dari 3 perlakuan (A=2%, B=3%, dan C=4%) dan kontrol dengan 3 kali ulangan. Hasil penelitian diperoleh hasil kandidat vaksin inaktif bakteri A.hydrophila sebanyak 9 ml/tabung falcon. Infeksi bakteri A. hydrophila dengan kepadatan 109 cfu/ml sebanyak 0,1 ml dengan masa pemeliharaan 1 minggu ditandai terjadi pengerasan di bagian perut dan terdapat kerusakan pada sirip punggung pada hari ketujuh. Hasil uji viabilitas menunjukkan tidak terdapat pertumbuhan koloni bakteri A. hydrophila dibandingkan dengan kontrol. Hasil uji adhesi sel epitel usus pada kontrol dan perlakuan A, B, C diperoleh rata-rata 10.3, 0.73, 0.43, dan 0.23 bakteri/10 sel epitel. Analisis uji ANOVA diperoleh nilai uji F Hitung > F Tabel, artinya perlakuan memberikan pengaruh sangat berbeda signifikan antar semua perlakuan. Hasil analisa regresi diperoleh (R2) sebesar 0.66 bahwa hubungan keterkaitan antara vaksin inaktif konsentrasi formalin berbeda dengan jumlah bakteri A. hydrophila yang menempel pada sel epitel sebesar 66%.   One preventive effort to tackle diseases in fish is by boosting immunity, such as through vaccination, as increased immunity in fish can prevent disease infections. Efforts have been made to develop effective immunization programs using vaccines made from whole bacterial cells inactivated using formalin and/or heat. The objective of this study was to determine the results of viability tests and intestinal epithelial cell adhesion tests using formalin-inactivated vaccines with different concentrations. The method used in this study was an experimental approach with a completely randomized design (CRD) consisting of three treatments (A=2%, B=3%, and C=4%) and a control, each with three replications. The study results showed that the candidate inactivated vaccine of A. hydrophila bacteria was obtained at 9 ml/falcon tube. A. hydrophila bacterial infection with a density of 10⁹ CFU/ml at 0.1 ml and a maintenance period of one week was characterized by abdominal hardening and dorsal fin damage on the seventh day. The viability test results showed no bacterial colony growth of A. hydrophila compared to the control. The intestinal epithelial cell adhesion test results for the control and treatments A, B, and C showed average bacterial adhesion of 10.3, 0.73, 0.43, and 0.23 bacteria/10 epithelial cells, respectively. ANOVA test analysis showed that the calculated F-value was greater than the table F-value, indicating a highly significant effect among all treatments. Regression analysis resulted in an R² value of 0.66, suggesting that 66% of the variation in the adhesion of A. hydrophila bacteria to epithelial cells was associated with different formalin concentrations in the inactivated vaccine.
EFECT OF LASERPUNCTURE INDUCTION TO INCREASE GSI AND HSI MALE CATFISH BROODSTOCKS Hariani, Dyah; Purnama, Erlix Rachmad; Purnama, Tarsan; Fadjar, Mohamad
JFMR (Journal of Fisheries and Marine Research) Vol. 4 No. 1 (2020): JFMR
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2020.004.01.11

Abstract

Induction of laser puncture in the male catfish broodstock has a positive influence by increasing reproductive activity which is very helpful for the process of gonadal maturation. The contribution of laser puncture induction in accelerating gonadal maturation can be seen with an increasing in the value of Gonado Somatic Index (GSI) and Hepato Somatic Idex (HIS) indicators. The purpose of this study was to examine the effect of laser puncture induction on increasing the value of GSI and HIS male catfish broodstock. This experimental study used 48 broodstocks of one year old male catfish with a body weight of 1000-1200 g maintained in a concrete pond. The treatment consisted of 2 groups i.e. without induction and induction of laser puncture, each treatment was repeated 4 times. Laser puncture induction was performed every 15 days at the point of reproduction for 15 seconds until the 75th day. The parameters observed were GSI and HIS. The results showed that there was a highly significant increase (P <0.002) in the value of GSI and HSI in male male catfish broodtock after induced by laser puncture. Conclusion for this research was laser punctur induction at the point of reproduction in male catfish can accelerate gonadal maturation with indicators of increasing GSI and HIS values. 
TINGKAT TRANSFER OKSIGEN KINCIR AIR SELAMA PERIODE BLIND FEEDING BUDIDAYA INTENSIF UDANG PUTIH (Litopenaeus vannamei) Ariadi, Heri; Wafi, Abdul; Fadjar, Mohamad; Mahmudi, Mohammad
JFMR (Journal of Fisheries and Marine Research) Vol. 4 No. 1 (2020): JFMR
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2020.004.01.2

Abstract

Efektifitas penggunaan kincir air selama masa periode blind feeding budidaya intensif udang vannamei (Litopenaeus vannamei) merupakan salah satu kegiatan penting yang harus diperhatikan demi kelangsungan siklus budidaya yang stabil. Tujuan dari penelitian ini adalah untuk mengetahui tingkat transfer oksigen oleh kincir air serta hubunganya terhadap parameter harian kualitas air tambak. Peneltian ini dilakukan dengan konsep desain kausal ex-post facto selama 30 hari awal masa budidaya intensif udang vannamei (Litopenaeus vannamei) atau selama periode blind feeding budidaya. Hasil penelitian menunjukan bahwa nilai parameter kualitas air di lokasi tambak masih sesuai ambang batas. Sedangkan tingkat transfer oksigen oleh kincir air bersifat fluktuatif selama 30 hari masa pengamatan. Sementara dari hasil uji korelasi menunjukan bahwa hanya variabel suhu dan salinitas air memiliki keeratan hubungan yang kuat terhadap proses transfer oksigen oleh kincir. Sehingga dapat disimpulkan bahwa fluktuasi tingkat transfer oksigen oleh kincir air akan berpengaruh ataupun dipengaruhi oleh kondisi suhu dan salinitas air tambak.
Co-Authors Abdul Rahem Faqih Abdul Wafi Abdul Wafi, Abdul Andi Al Furqan Andi Kurniawan Arief Prajitno, Arief Arning Wilujeng Ekawati Asep Awaludin Prihanto Asus Maizar Suryanto H Attabik M. Amrillah Attabik Mukhammad Amrillah Ayu Winna Ramadhani BAGUS DWI HARI SETYONO Bimo Aji Nugroho Diana Aisyah Dwi Retno Andriani Dyah Hariani Happy Nursyam Heri Ariadi Herlina, Cucun Isroni, Wahyu Jefri Anjaini Jusril, Muhammad Maftuch Maftuch Mohammad Mahmudi Mr. I Gede Eko Putra Sri Sentanu Ni Nyoman Dian Martini Novalina Serdiati Nuri Muahiddah Pramudia, Zulkisam Purnama, Erlix Rachmad Purnama, Tarsan R Adharyan Islamy Rangga Idris Affandi Rangkuti, Rizky Fadilla Agustin Rizal Rifa'i, Ramanda Ahmad Sekartadji, Pranawengrum setya widi ayuning permanasari Sri Andayani Sri Sentanu, I Gede Eko Putra Sukoso Sukoso Supriatna Tohap Simangunsong Valina, Rahmi Veryl Hasan Wibowo, Jonathan Christian Yuni Kilawati Yunita Maimunah