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Sorbet Made from Moringa Leaves and Red Guava as an Alternative for the Management of Iron Deficiency Anemia in Adolescent Girls Arviyani, Tiffany Nisa; Afifah, Diana Nur; Noer, Etika Ratna; Rahfiludin, M. Zen; Mahati, Endang
Journal of Applied Food Technology Vol 9, No 2 (2022)
Publisher : Dept. Food Technology, Faculty of Animal and Agricultural Sciences, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17728/jaft.15782

Moringa leaves (Moringa oleifera) contain high iron (Fe) but low bioavailability of Fe because it contains calcium and tannins. Vitamin C in red guava fruit (Psidium guajava) can increase Fe absorption. Sorbet made from red guava and Moringa leaves can be an alternative to overcome iron deficiency anemia in adolescent girls. This study aims to analyze the level of acceptance, levels of Fe, Ca, vitamin C, and tannins, as well as the bioavailability of Fe sorbet made from red guava and Moringa leaves. Sorbet consists of 5 formulas including, F1 (strawberry sorbet), F2 (red guava sorbet), F3 (Moringa leaf sorbet), F4 (red guava sorbet + 18% Moringa leaves), and F5 (red guava sorbet + 22% Moringa leaves). Acceptance level was tested with the hedonic method (appearance, smell, taste, and texture) and statistically analyzed using Friedman and Wilcoxon test. The Fe and Ca were analyzed using X-Ray Fluorescence (XRF) method, vitamin C and tannins were analyzed using High-Performance Liquid Chromatography (HPLC) and spectrophotometry methods. Fe bioavailability test was performed using the inverted bowel bag method. The level of preference for each attribute was significantly different (p < 0.05). The level of preference chosen was F2. The mean levels of Fe, Ca, vitamin C, and tannins in F4 were 0.95 ± 0.14, 5.91 ± 0.73, 26.29 ± 0.13, and 135.65 ± 0.91 mg/100 g. The mean levels of Fe, Ca, vitamin C, and tannins in F5 were 0.21 ± 0.29, 6.03 ± 0.67, 46.72 ± 0.69, and 87.76 ± 0.40 mg/100 g. The highest Fe bioavailability was F5 while the lowest was F3. The best formula based on the parameters of preference, Fe, Ca, vitamin C, and tannin levels, as well as Fe bioavailability, was sorbet F5.

Potensi dan Mekanisme Kacang Tropis terhadap Sindrom Metabolik: Tinjauan Literatur: The Potential Benefits and Mechanism of Action of Tropical Nuts Against Metabolic Syndrome: A Literature Review Setyawati, Amalia Rani; Anjani, Gemala; Mahati, Endang
Amerta Nutrition Vol. 8 No. 3 (2024): AMERTA NUTRITION (Bilingual Edition)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/amnt.v8i3.2024.480-495

Background: Metabolic syndrome is a significant risk factor for both type 2 diabetes mellitus and cardiovascular disease, with a high prevalence in Asia Pacific, particularly in Indonesia. To reduce its prevalence, several studies have recommended the use of tropical nuts, which can be developed as functional foods and complementary treatment. In this context, the bioactivities of tropical nuts can largely be attributed to their rich content of monounsaturated fatty acids, polyunsaturated fatty acids, fiber, minerals, vitamins, phytosterols, and polyphenols. Objectives: This literature review aims to evaluate the potential benefits and mechanism of action of tropical nuts against metabolic syndrome. Methods: The study design was a literature review of several articles from 3 online databases, including PubMed, Google Scholar, and ScienceDirect. Discussions: The results showed that tropical nuts (peanut, sacha inchi, cashew, tropical almond, and Brazil nut) had several biologically active components, such as arginine, fiber, fatty acid, mineral, vitamin, phenolic compounds, resveratrol, and phytosterol. The test samples were reported to have the ability to modulate Nrf2, SOD, MDA, GSH, GPx, and CAT due to their antioxidant activity. In inflammation, tropical nuts had a significant effect on NF-κB, NLRP3, TNF-ɑ, IL-8, IL-1ꞵ, IL-6, and IL-10. The results also showed their ability to enhance lipid synthesis, nitric oxide production, advanced glycation end-product, prostaglandin, SIRT3, homocysteine, protein kinase C, adhesion molecules, platelet aggregation, GLP-1, PYY, AGRP, PPARɑ/ꞵ/δ, GLUT4, and insulin receptor. Conclusions: Tropical nuts had beneficial effects on metabolic syndrome due to their bioactivities, including antioxidants, anti-inflammatory, anti-obesity, antidiabetic, antihypertensive, anti-dyslipidemia, and cardioprotective.

The Effect of Sacha Inchi Tempe on Blood Glucose, HOMA-IR, and TNF-ɑ in Rats with Metabolic Syndrome Setyawati, Amalia Rani; Anjani, Gemala; Mahati, Endang; Afifah, Diana Nur; Syauqy, Ahmad; Astawan, Made; Rahmawati, Irma Sarita
Jurnal Gizi dan Pangan Vol. 19 No. 2 (2024)
Publisher : The Food and Nutrition Society of Indonesia in collaboration with the Department of Community Nutrition, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25182/jgp.2024.19.2.97-106

This research aimed to evaluate the impact of sacha inchi tempe (Plukenetia volubilis L.) on Fasting Blood Glucose (FBG), Homeostatic Model Assessment for Insulin Resistance (HOMA-IR), and Tumor Necrosis Factor Alpha (TNF-ɑ) levels. In addition, metabolic syndrome was induced in 36 male Wistar rats aged 2 months at 150–200 g weight by giving a High-Fat High-Fructose diet (HFFD) for 2 weeks. The extract was administered through oral gavage in dose-dependent manner and rats were allocated into 6 groups, namely: 1). Normal control or K0; 2). Negative control or K-; 3). Positive control or K+ with 0.18 mg/200 g BB of simvastatin; 4). Intervention with 0.9 g sacha inchi tempe or P1; 5). Intervention with 1.8 g sacha inchi tempe or P2, and; 6). Intervention with 3.6 g sacha inchi tempe or P3. Meanwhile, normal chow rats were used and served as the control group. After 2 and 5 weeks of induction and intervention, blood was drawn to determine FBG. Blood insulin was examined after 5 week of intervention. Rats were euthanized at the end of the intervention for hepatic TNF-α analysis before calculating HOMA-IR. The result showed that there was a significant difference (p<0.05) in FBG, HOMA-IR and hepatic TNF-α levels after sacha inchi tempe treatment. Rats receiving the highest dose of sacha inchi tempe had the most significant reduction (p<0.05) in FBG, HOMA-IR and hepatic TNF-α, when compared to simvastatin group. Therefore, sacha inchi tempe could attenuate glycemic and inflammation profiles in metabolic syndrome.

Effects of Sacha Inchi Tempeh on Oxidative Stress in Rats with Metabolic Syndrome Nafisah; Anjani, Gemala; Afifah, Diana Nur; Syauqy, Ahmad; Mahati, Endang; Astawan, Made; Rahmawati, Irma Sarita
Indonesian Journal of Human Nutrition Vol. 11 No. 2 (2024)
Publisher : Department of Nutrition, Faculty of Health Sciences, Universitas Brawijaya Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.ijhn.2024.011.02.1

Metabolic syndrome is a condition caused by metabolic disorders such as overweight, obesity, physical inactivity, and genetic factors. Increased reactive oxygen species (ROS) production in metabolic syndrome causes oxidative stress, characterized by decreased antioxidant capacity, increased malondialdehyde (MDA), and decreased superoxide dismutase (SOD) enzymes. Sacha inchi has a high alpha-linolenic acid (ALA) content, tocopherols, and antioxidants that can help protect liver tissue from damage caused by oxidative stress in metabolic syndrome. This study aimed to evaluate how sacha inchi tempeh affects the levels of MDA, SOD enzyme, and total antioxidant capacity (TAC) in Wistar rats with metabolic syndrome induced by a high-fat high-fructose diet (HFFD). The study used a true experimental pre-and post-control group design on rats with the intervention of sacha inchi tempeh (0.9 g, 1.8 g, 3.6 g) for 5 weeks (35 days). The ELISA (Enzyme-Linked Immunosorbent Assay) method with the FineTest procedure was used to measure levels of TAC, MDA, and SOD enzymes. The results of paired T-tests showed a significant difference in TAC levels before and after the intervention in the intervention group compared to the healthy group (p<0.05). MDA and SOD enzyme levels in rat liver tissue also improved significantly (p<0.05). Compared to simvastatin medication therapy, sacha inchi tempeh at a dose of 3.6 g had the best results. Therefore, sacha inchi tempeh is beneficial as a nutraceutical meal in reducing oxidative stress in individuals suffering from metabolic syndrome.

Effects of Sacha Inchi Tempeh on Oxidative Stress in Rats with Metabolic Syndrome Afifah, Diana Nur; Nafisah; Anjani, Gemala; Syauqy, Ahmad; Mahati, Endang; Astawan, Made; Rahmawati, Irma Sarita
Indonesian Journal of Human Nutrition Vol. 11 No. 2 (2024): In Press
Publisher : Department of Nutrition, Faculty of Health Sciences, Universitas Brawijaya Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar

Metabolic syndrome is a condition caused by metabolic disorders such as overweight, obesity, lack of physical activity, and genetic factors. Increased ROS production in metabolic syndrome causes oxidative stress characterized by decreased antioxidant capacity, increased MDA, and decreased SOD enzymes. Sacha inchi has a high ALA content that can increase omega-3 levels, tocopherols, and antioxidants that can help protect liver tissue from damage caused by oxidative stress in metabolic syndrome.The purpose of this study was to look at how sacha inchi tempeh affected the levels of malondialdehyde, superoxide dismutase enzyme, and overall antioxidant capacity in rats with metabolic syndrome brought on by a diet high in fat and fructose. The method used in the research is an experiment with a pre-and post-control group design on Wistar strain white rats with metabolic syndrome induced by HFFD, with the intervention of sacha inchi tempe (0.9 g; 1.8 g; 3.6 g) for 5 weeks (35 days). The results of the study with paired T-tests were that there was a significant difference in TAC levels before and after the intervention in the intervention group with a p-value of <0.05 compared to the healthy group. MDA and SOD enzyme levels in rat liver tissue also were significant improvement with a p-value of <0.05. When compared to simvastatin medication therapy, sacha inchi tempeh at a dose of 3.6 g had the best results. Therefore, it is established that sacha inchi tempeh is beneficial as a nutraceutical meal in reducing oxidative stress in individuals suffering from metabolic syndrome.

Liprotide-encapsulated vitamin D3 modulates circulated PTH levels and improved bone microstructure Shauma, Claradhita Ayu; Fulyani, Faizah; Pramono, Adriyan; Mahati, Endang; Putri, Sylvia Rahmi; Maulana, Reza Achmad; Anjani, Gemala
Jurnal Gizi Indonesia (The Indonesian Journal of Nutrition) Vol 12, No 1 (2023): December
Publisher : Department of Nutrition Science, Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jgi.12.1.44-52

Background: vitamin D (25(OH)D) is a fat-soluble vitamin that is unstable in the gastrointestinal environment and has low bioavailability. A protein-lipid complex (liprotide) can be used as a shell to increase vitamin D stability and bioavailability. Liprotide can also serve as a delivery system for transporting vitamin D to its intended site. Little attention has been paid to utilizing liprotide as a delivery system for vitamin D and evaluating its functional activity.Objective: to investigate the effect of liprotide-encapsulated vitamin D3 on PTH levels and bone microstructure in vitamin D and calcium (VD-Ca) deficient rats.Materials and Methods: an overall of 24 Wistar rats had been divided into four groups, a normal control group (K), a VD-Ca group without treatment (K-), a VD-Ca group with 180 IU/200 gBW/day free vitamin D3 (FVD3), and a VD-Ca group with 180 IU/200 gBW/day liprotide-encapsulated vitamin D3 (LVD3). Before and after 28 days of vitamin D intervention, blood samples were taken and analysed for serum PTH levels. The microstructure of the bone was analyzed using the Scanning Electron Microscope (SEM).Results: the VD-Ca rats supplemented with vitamin D3 (FVD3 and LVD3) had a significant decrease in serum PTH levels (p<0.001) and improved bone microstructure (p<0.05) compared to the (K-) group. The reduction of PTH in the LVD3 group was higher compared to the FVD3 group. The bone microstructure between the FVD3 and LVD3 groups is significantly different, as seen in the Ct.Wi parameter, with the LVD3 group having a higher Ct.Wi than the FVD3 group.Conclusion: liprotide-encapsulated vitamin D3 improves the serum PTH level and bone microstructure in a rat model of vitamin D and calcium deficiency.

Prognostic Significance of the Epithelial–Mesenchymal Transition Phenotype in Basal Cell Carcinoma: A Meta-Analysis of E-Cadherin Loss and Stromal Alpha-SMA Upregulation as Recurrence Predictors Meira Astuti; Endang Mahati; Udadi Sadhana; Selamat Budijitno; Ign Riwanto
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 10 No. 3 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v10i3.1537

Background: Basal cell carcinoma represents the most prevalent cutaneous malignancy worldwide. While metastasis is rare, local recurrence poses a substantial therapeutic challenge, particularly in the anatomically critical H-zone of the face. Conventional risk stratification relies on tumor size and histological subtype, but these markers frequently fail to capture the intrinsic biological aggressiveness of the tumor. The epithelial–mesenchymal transition phenotype, characterized by the loss of epithelial adhesion molecule E-cadherin and the activation of the tumor stroma via alpha-smooth muscle actin expression, has emerged as a potential driver of local invasion. Methods: We conducted a systematic review and meta-analysis adhering to PRISMA 2020 guidelines to evaluate the prognostic value of these biomarkers. A comprehensive search identified ten pivotal studies comprising 648 cases. The primary endpoint was adverse outcome, defined as clinical recurrence or the presence of high-risk infiltrative histology. Data were synthesized using a random-effects model to calculate pooled Odds Ratios and Standardized Mean Differences, with rigorous sensitivity analyses to account for heterogeneity. Results: The meta-analysis revealed a profound association between stromal activation and adverse outcomes. Alpha-SMA upregulation was the most robust predictor, with a pooled Odds Ratio of 6.82 (95% CI: 3.14–14.81; p < 0.0001). Loss of membranous E-cadherin also significantly predicted recurrence (Odds Ratio = 4.15; 95% CI: 1.89–9.10; p = 0.0004), although with higher heterogeneity, reflecting the focal nature of partial epithelial–mesenchymal transition at the invasive front. The combined phenotype of high alpha-SMA and low E-Cadherin represented the highest risk profile. Conclusion: The epithelial–mesenchymal transition phenotype serves as a high-fidelity predictor of basal cell carcinoma recurrence. Stromal alpha-SMA marks a permissive soil for invasion and should be considered for integration into pathological reporting for ambiguous or high-risk tumors to guide surgical margin management.

Prognostic Significance of the Epithelial–Mesenchymal Transition Phenotype in Basal Cell Carcinoma: A Meta-Analysis of E-Cadherin Loss and Stromal Alpha-SMA Upregulation as Recurrence Predictors Meira Astuti; Endang Mahati; Udadi Sadhana; Selamat Budijitno; Ign Riwanto
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 10 No. 3 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v10i3.1537

Background: Basal cell carcinoma represents the most prevalent cutaneous malignancy worldwide. While metastasis is rare, local recurrence poses a substantial therapeutic challenge, particularly in the anatomically critical H-zone of the face. Conventional risk stratification relies on tumor size and histological subtype, but these markers frequently fail to capture the intrinsic biological aggressiveness of the tumor. The epithelial–mesenchymal transition phenotype, characterized by the loss of epithelial adhesion molecule E-cadherin and the activation of the tumor stroma via alpha-smooth muscle actin expression, has emerged as a potential driver of local invasion. Methods: We conducted a systematic review and meta-analysis adhering to PRISMA 2020 guidelines to evaluate the prognostic value of these biomarkers. A comprehensive search identified ten pivotal studies comprising 648 cases. The primary endpoint was adverse outcome, defined as clinical recurrence or the presence of high-risk infiltrative histology. Data were synthesized using a random-effects model to calculate pooled Odds Ratios and Standardized Mean Differences, with rigorous sensitivity analyses to account for heterogeneity. Results: The meta-analysis revealed a profound association between stromal activation and adverse outcomes. Alpha-SMA upregulation was the most robust predictor, with a pooled Odds Ratio of 6.82 (95% CI: 3.14–14.81; p < 0.0001). Loss of membranous E-cadherin also significantly predicted recurrence (Odds Ratio = 4.15; 95% CI: 1.89–9.10; p = 0.0004), although with higher heterogeneity, reflecting the focal nature of partial epithelial–mesenchymal transition at the invasive front. The combined phenotype of high alpha-SMA and low E-Cadherin represented the highest risk profile. Conclusion: The epithelial–mesenchymal transition phenotype serves as a high-fidelity predictor of basal cell carcinoma recurrence. Stromal alpha-SMA marks a permissive soil for invasion and should be considered for integration into pathological reporting for ambiguous or high-risk tumors to guide surgical margin management.

In Silico of AMHRII Receptor Binding and Toxicity Prediction of Clitoria ternatea Kurniawati, Erna Yovi; Mahati, Endang; Zaw Myo Hein
Window of Health : Jurnal Kesehatan Vol 7 No 4 (October 2024)
Publisher : Fakultas Kesehatan Masyarakat Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/woh.v7i4.1166

Clitoria ternatea compounds show significant potential as herbal drug candidates for polycystic ovary syndrome (PCOS) through improving anti-Müllerian hormone (AMH) parameters. This study aims to evaluate the potential of these compounds in binding to AMHRII receptors using an in silico approach. Several compounds, baicalein, isorhamnetin, and malvidin, showed high binding affinity towards AMHRII receptors, approaching or even surpassing some control drugs. These compounds have favorable toxicity profiles, with high LD50s, suggesting low toxicity and high safety potential for therapeutic use. Some compounds, such as isorhamnetin, termination, petunidin, malvidin, cyanidin, luteolin, and protocatechuic acid, have an unfavorable number of hydrogen donors, as does gallic acid, which has an unfavorable number of hydrogen acceptors. The development of technologies that improve bioavailability and pharmacokinetics can overcome these challenges. Further studies in vivo are needed to confirm the effectiveness and safety of these compounds in potential clinical therapies and develop innovative herbal therapies for Clitoria ternatea-based PCOS.

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