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All Journal JURNAL KIMIA SAINS DAN APLIKASI Jurnal Pengajaran MIPA EDUSAINS Abdimas Jurnal Inovasi Pendidikan Kimia Jurnal Bahan Alam Terbarukan Rekayasa: Jurnal Penerapan Teknologi dan Pembelajaran Sainteknol : Jurnal Sains dan Teknologi Chemistry in Education Indonesian Journal of Chemical Science ALCHEMY Jurnal Penelitian Kimia Indonesian Journal of Chemistry Communications in Science and Technology International Journal of Active Learning Abdimasku : Jurnal Pengabdian Masyarakat Edukimia Jurnal Pengabdian Kepada Masyarakat (JPKM) Tabikpun International Journal of Research Innovation and Entrepreneurship Indonesian Journal of Chemical Science Rekayasa: Jurnal Penerapan Teknologi dan Pembelajaran
F Widhi Mahatmanti
Department Of Chemistry, Faculty Of Mathematics And Natural Sciences, Semarang State University, Jl. Raya Sekaran Gunungpati Semarang 50229
Arts Humanities Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Industrial & Manufacturing Engineering Materials Science & Nanotechnology Physics Social Sciences Other

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Chitosan/Anthocyanin-Based Indicator Film with Carnauba Wax Addition for Monitoring Chicken Freshness Roichatuttamamah, Wirda; Mahatmanti, F. Widhi; Kusuma, Samuel Budi Wardhana; Rahayu, Endah Fitriani
Jurnal Kimia Sains dan Aplikasi Vol 28, No 6 (2025): Volume 28 Issue 6 Year 2025
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jksa.28.6.283-291

Abstract

Indicator films can be applied to food packaging to monitor the quality of food products. However, chitosan/anthocyanin films tend to have low hydrophobicity, which may compromise their performance as indicators and lead to dye migration. One approach to address this issue is by incorporating additional materials such as carnauba wax. This study investigates the effect of carnauba wax addition on the mechanical properties and water contact angle of chitosan/anthocyanin-based indicator films, as well as evaluates the performance of the resulting films in monitoring the freshness of chicken meat. Indicator films were prepared by adding carnauba wax at concentrations of 6, 9, 12, and 15% (w/w of chitosan). The results showed that the addition of carnauba wax improved the mechanical properties of the films, although the improvement was not statistically significant. The best mechanical performance was observed with the 15% wax formulation. Additionally, the water contact angle increased with higher concentrations of carnauba wax, with the highest value (71.630°) observed for the CS/ABT/CW15% film. The indicator films were applied to chicken packaging and evaluated based on color changes during storage as a measure of freshness. After 30 hours, the film color changed from dark brown to green. The color change pattern during storage was similar for films with and without carnauba wax, indicating that the addition of carnauba wax does not affect the film’s ability to monitor chicken freshness.
Optimization of the Performance of a Microplate Type UV-Vis Spectrophotometer as a Protein Content Testing Instrument Based on Green Analytical Chemistry (GAC) Martin Sulistyani; Mahatmanti, F. Widhi; Huda, Nuril; Prasetyo, Ridho
Indonesian Journal of Chemical Science Vol. 14 No. 3 (2025): Indonesian Journal of Chemical Science
Publisher : Prodi Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/ijcs.v14i3.31948

Abstract

Advancements in laboratory technology have driven the adoption of more efficient and environmentally friendly analytical methods, in line with the principles of Green Analytical Chemistry (GAC). This study aims to optimize the performance of a microplate-based UV-Vis spectrophotometer for determining protein content in food samples using the Biuret method. The method is based on a colorimetric reaction between Biuret reagent and peptide bonds in proteins, forming a purple complex whose absorbance is proportional to protein concentration. Three dilution methods were evaluated: mL-microplate, µL-microplate, and mL-cuvette, based on parameters including maximum wavelength, linearity, and accuracy. All methods showed the same maximum absorbance wavelength (562 nm), indicating reaction stability. The mL-cuvette method achieved the highest linearity (R² = 0.998), while the mL-microplate method demonstrated the best accuracy (91.00%) and most consistent results in tempeh and yogurt samples. Meanwhile, the µL-microplate method offered acceptable accuracy (90.25%) with significantly reduced reagent volume, supporting the GAC approach. Therefore, the mL-microplate method is recommended as the primary technique for protein analysis using UV-Vis spectrophotometry, while the µL-microplate method provides an efficient and eco-friendly alternative for modern laboratory applications.
Co-Authors - Jumaeri A.A. Ketut Agung Cahyawan W Adhi, Nino Agung Tri Prasetya Agung Tri Prasetya Ainul, Ahmad Akhib Aisyah, Amma Aisyah, Amma Alighiri, Dante Andri Cahyo Kumoro Anindya, Bella Diska Aprilia, Nurkintan Arie Fitry Apriyanti, Arie Fitry Arum Mawar Wati Astuti, Indri Puji Atika Rapierna, Atika Bahron, Hadariah Basir, Ikhsan Fadilah Basir, Ikhsan Fadilah Cepi Kurniawan Cindy Dwi Herawan, Cindy Dwi Deviani, Siska Shelvia Deviani, Siska Shelvia Dewi, Nuriana Rahmani Dian Sri Asmorowati Didik Prasetyoko Edy Cahyono Eka Hartanti, Eka Eko Budi Susatyo Ella Kusumastuti, Ella Endang Susilaningsih Endang Susilowati Fitria, Reffy Ika Fivi Dessy Khodijah Hanis, Harjono Hanis, Harjono Haq, Ismi Arinal Harjito - Hayyu Fitriah, Hayyu Hilda Chalimatus SC, Hilda Chalimatus Hutasoit, Parpulungan Hutasoit, Parpulungan Indah Puji Lestari Laeli Kurniasari Latifah Latifah Libiyana Nurviantika, Libiyana Luluatul Maghfiroh, Luluatul M. Alauhdin, M. Mahardika Prasetya Aji Mahfudz, Johar Marsudi Marsudi Martien Herna Susanti Martin Sulistyani Maulida Zakia Melykhatun, Rizka Ayu Mika Septiawan, Mika Miranda Oxtariani Moch. Setyadji Moh Djaeni Mohammad Alauhdin, Mohammad Muryani Dinata, Muryani Naftalina Ulik Adhelia Nanik Wijayati Narsito Narsito Narsito Narsito Noviana, Melly Nugraha, Muhammad Septian Nugraha, Muhammad Septian Nugraha, Rizki Nuni Widiarti Nuril Huda Nurmala, Novera Ayang Nurmala, Novera Ayang Nuryono Nuryono Nuryono Nuryono octavia uriastanti, octavia Patricia, Amelinda Diana Patricia, Amelinda Diana Persada, Mega Bunga Persada, Mega Bunga Prasetyo, Ridho Pratiwi, Khana Fitri Pratomo, Sigit Wahyu Pratomo, Sigit Wahyu PUJI LESTARI Putra, Rizki Suhendar Putri, Syarafina Dirinda Qonita, Ulya Rachmadiyono, Dwi Rahayu, Endah Fitriani Rahmalia, Berliana Putri Ramadhan, Dinar Alghifari Ratri, Irwandari Rahma Nur Ratri, Irwandari Rahma Nur Renni, Chayun Pida Renni, Chayun Pida Rini Susanti Wulandari Rizki Yuniarti, Rizki Roichatuttamamah, Wirda Rusadi, Eka Rusadi, Eka Samuel Budi Wardhana Kusuma Sari, Erlin Purwita Sari, Erlin Purwita Sari, Nur Vita Sari, Nur Vita Senda Kartika Rakainsa Seta Kahardian Ardana, Seta Kahardian Setia Budi Sasongko Siti Maesaroh Siti Sundari Miswadi Sri Haryani Sri Mantini Rahayu Sedyawati, Sri Mantini Rahayu Sri Nurhayati Sri Susilogati Sumarti Sri Wahyuni SRI WARDANI Sudarmin - Sudarmin Sudarmin Sudibyo, Raden Sulistyani, Martin Suprapto, Surapto Sutikno Sutikno Syafi'i, Imam Triastuti Sulistyaningsih Wara Dyah Pita Rengga Wardani, Deta Sri Warlan Sugiyo Wati, Safna Rahma Widhihastuti, Endah Widiya Aprilianti Wisnu Sunarno Woro Sumarni Yogo Setiawan Yuan Maylia Rosanti Yuni Susanti, Devi