Article Per Year (5 Year)
p-Index From 2021 - 2026
2.195
P-Index
This Author published in this journals
All Journal Jurnal Keperawatan International Journal of Biosciences and Biotechnology Jurnal Ilmu Dasar BERKALA SAINSTEK EduChemia: Jurnal Kimia dan Pendidikan UNEJ e-Proceeding Jurnal Kimia Riset Jurnal Biodjati Jurnal Ilmiah Universitas Batanghari Jambi Indonesian Journal of Chemistry The Indonesian Journal of Health Science Abdimasku : Jurnal Pengabdian Masyarakat Indonesian Journal of Global Health research Bima Nursing Journal Indonesian Chimica Letters
Siswoyo Siswoyo
Chemistry Department, Faculty Of Mathematic And Natural Sciences, Universitas Jember, Jl. Kalimantan 37, Jember, East Java, 68121
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences

Published : 21 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 21 Documents
Search

 1   2   3 
An Extracellular Pectinase from ISH16 Bacteria Isolated Induced by Coffee Pulp Waste Substrate Kahar Muzakhar; Farah Salma Elida; Ramdhan Putrasetya; Siswoyo Siswoyo; Rudju Winarsa; Hidayat Teguh Wiyono
Jurnal Biodjati Vol 7 No 2 (2022): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i2.20279

Abstract

An α-1,4-glycosidic bonds galactoses pectin, mainly composed of a D-galacturonic acid chain, are important biomaterial widely used in industries. Utilizing this material, a bioprocess, including the biocatalysis pectinase, is often needed. Pectinase production was optimized in 7 days SSF at 37°C, and the pectinase activities were daily measured by the method of Somogy-Nelson. The optimum pectinase production was 0.166 U/ml on the fourth day SSF. Purification using open column ion exchange chromatography DEAE cellulose DE-52 resulted in 1030.9 folds of pectinase purity with a yield of 25.9%. The enzyme was at optimal activity at pH six and attended stable in the pH range of 5.5-8, while optimal activity at a temperature of 50°C and was stable in the range of 30-45°C. The pectinase activity increased by 120% with the addition of 10 mM Mg2+, and 95% retained when 10 mM Ca2+ was added. The presence of 10 mM Na+, K+, and Fe2+ resulted in a slight effect of activity at 85%, 83%, and 78%. However, it was strongly inhibited by 10 mM Al3+ and retained 25%. Based on the results above, the microbial utilization of coffee pulp waste by ISH16 bacteria pectinolytic is one opportunity to produce valuable pectinase with low-cost production, so comprehensive examination in large-scale production is needed too. In this paper, all research detail steps were described.
Co-Authors Agus Subekti Ahmad Fathoni Akhmad Zainur Ridla Alvin Faizin Lisar Anggia Rose Sukaton Anida, Choirun Nisa Aqidatun Ayu Wulandari Ardyanti, Zona Salsabila Asnawati Asnawati Asnawati Asnawati Atik Komaria Bambang Piluharto Baskoro Setioputro Cholil Albarizi Dhimas Rizky Handoko Elly Nurus Sakinah, Elly Nurus Esti Utarti Farah Salma Elida Farel Govind Alfayed Ariyanto Fenly Arismaya Hafan Sutawardana, Jon Heri Siswanto Hidayat Teguh Wiyono Indradewa, Rhian Kadek Novi Andani Kahar Muzakhar Kiki Puji Setianingrum Kushariyadi Kushariyadi Kushariyadi Kushariyadi Maulidah Muflihah, Yeni Mintadi, Mukh. Muhammad Reza Mulia Hakam Mulyono, Tri Neran Neran Neran Neran Nistiandani, Ana Nur Widayati Putri F. Rianasari Qurrota Ayun Ramdhan Putrasetya Rondhianto Rondhianto Setyo Bagus Hariyanto Sinta Wijayanti Surya Primaswara, Alfisa Susi Nurqomariyah Sutoyo S Syahrul Abdul Yazid Tanti Haryati Tira Anjeli Rahmah Trio F. Nugroho Wantiyah Wantiyah Wijayanti, Chatarina Winarsa, Rudju Yeni Maulidah Muflihah Zahratul Umniyyah Zein, Inayatul Aulia Rizka Zulfikar Zulfikar