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All Journal AL KAUNIYAH Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Indonesian Journal of Forestry Research BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology ANNALES BOGORIENSES Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Jurnal Riset Biologi dan Aplikasinya Jurnal Ilmiah Matematika Makara Journal of Science Makara Journal of Technology Annales Bogorienses Berita Biologi
WIBOWO MANGUNWARDOYO
Departemen Biologi FMIPA-UI
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Electrical & Electronics Engineering Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology

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Journal : AL KAUNIYAH

 1 
OPTIMASI DAN PEMEKATAN LIPASE Bacillus halodurans CM1 Aisyah, Arina; Mangunwardoyo, Wibowo; Trismilah, Trismilah; Suhendar, Dadang
Al-Kauniyah: Jurnal Biologi Vol 10, No 2 (2017): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (570.189 KB) | DOI: 10.15408/kauniyah.v10i2.4908

Abstract

Abstrak Lipase diketahui memiliki peranan penting dalam bidang industri. Produksi lipase dapat dihasilkan oleh kapang, khamir, dan bakteri. Penelitian bertujuan untuk meningkatkan aktivitas lipase yang dihasilkan oleh Bacillus halodurans CM1. Aktivitas lipase dapat ditingkatkan dengan optimasi komposisi media, mutasi bakteri dengan radiasi gamma dan N-methyl-N’-nitro-N-nitrosoguanidine (NTG). Enzim yang dihasilkan dipekatkan dengan metode stirred-cell ultrafiltration (UF)-ammonium sulfat dan UF-Polyethylene glycol (PEG). Uji aktivitas dilakukan pada tujuh media yang berbeda untuk mendapatkan media produksi. Delapan variabel komposisi media dioptimasi dengan rancangan Plackett-Burman. Bakteri dimutasi dengan radiasi gamma dosis 0,1–0,4 kGy dan NTG 0,05–0,15 mg/mL dengan waktu inkubasi 1–3 jam. Hasil penelitian menunjukkan bahwa media produksi yang digunakan berdasarkan optimasi media dan komposisi media Plackett-Burman adalah media dasar Bora & Bora yang mengandung 0,5% palm oil (PO) dan 0,09% CaCl2. Aktivitas lipase optimal diproduksi oleh bakteri hasil mutasi dengan NTG 0,1 mg/mL yang diinkubasi selama 3 jam. Pemekatan enzim UF-ammonium sulfat dan UF-PEG mampu meningkatkan aktivitas enzim lipase sebesar 18,44%.  Abstract Lipase is known to have an important role in the industrial field. Lipase can be produced by molds, yeasts, and bacteria. The research aimed to increase the activity of lipase produced by Bacillus halodurans CM1. Lipase activity can be improved by optimization of the composition of the media, the mutation of bacteria with gamma radiation and N-methyl-N'-nitro-N-nitrosoguanidine (NTG). The enzyme was concentrated by stirred-cell ultrafiltration method (UF)-ammonium sulfate and UF-Polyethylene glycol (PEG). The activity test was performed on seven different media to get production media. The eight variables of the media composition were optimized by Plackett-Burman design. The bacteria were subject to mutation by using 0.1–0.4 kGy dose of gamma radiation and 0.05–0.15 mg/mL NTG with incubation time for 1–3 hours. The results showed that the production media used based on optimization and composition of Plackett-Burman media was Bora Bora medium that containing 0.5% palm oil (PO) and 0.09% CaCl2. Optimum lipase activity was produced by the bacterium that mutated with 0.1 mg/mL NTG, incubated for 3 hours. The concentrated by UF-ammonium sulfate and UF-PEG could increase the lipase activity by 18.44%.
Methane Gas Production In Buffalo Rumen Fluid Containing Citronella Residue (Cymbopogon nardus L.) by In Vitro Method Sari, Arina Findo; Mangunwardoyo, Wibowo; Setiawan, Fery Hadi; Sugoro, Irawan
Al-Kauniyah: Jurnal Biologi Vol. 18 No. 2 (2025): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v18i2.40935

Abstract

Methane is one of the greenhouse gases produced by ruminants. One way to reduce methane is by feeding development strategies. Concentrates or forage are two possible forms of animal feed. Forage can be replaced with citronella (Cymbopogon nardus L.) residue, which has not been fully consumed as feed. The purpose of the study was to decide the production of methane gas that has residual citronella to concentrate in buffalo rumen fluid in vitro, for 48 hours. The treatments in this research were 0.4 g citronella residue (A); 0.4 g citronella residue + 0.01 g concentrate (B); 0.4 g citronella residue + 0.02 g concentrate (C); 0.01 g concentrate (D); and 0.02 g concentrate (E). For the 48-hour measurements, the highest methane gas production was treatment 98.2% (D); followed by 92.06% (E); 17.71% (C); 15.33% (A); and 13.54% (B). It can be concluded that methane gas can be reduced by residue citronella. This shows that citronella residue can lower methane gas. The study's findings are anticipated to be among the references for using agricultural product residues, particularly citronella as animal feed to reduce the methane produced by the livestock industry
Co-Authors Abinawanto Abinawanto Achmad Dinoto Aisyah, Arina Andi Salamah ARIYANTI OETARI Atit Kanti Bustamam, Alhadi Caniago, Asnany Caterina A, Ines Irene Chandra, Nicholas Dwi Desyandri Desyandri Ekowati Chasanah Endang Sri Heruwati Endang Sukara Fitri, Reno Gintung Patantis Guswenrivo, Ikhsan I Made Sudiana Idris Idris Ifah Munifah Ikhwani, Azra Zahrah Nadirah INDRAWATI GANDJAR Indriawan, Aris Indriawan, Aris Irawan Sugoro Kafi, Rahmat Al Lily Ismaini Lusini, Yuyun Napitupulu, Toga Pangihotan Pratama, Aulia Brellian Pratitis, Asri PUSPITA LISDIYANTI Sari, Arina Findo Setiawan, Fery Hadi SHANTI RATNAKOMALA Siswodarsono, Trismilah Siswodarsono, Trismilah Sita Heris Anita Sophia, Romauli Aya Srikandace, Yoice Suhendar, Dadang Suhendar, Dadang Sukma Nuswantara Sulistiani ., Sulistiani Sulistiani Sulistiani, Sulistiani Susanti, Agustina Eko Syafriana, Vilya Trismilah Trismilah YOPI YOPI Yuliar Yuliar Yusro Nuri Fawzya