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All Journal AL KAUNIYAH Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Indonesian Journal of Forestry Research BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology ANNALES BOGORIENSES Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Jurnal Riset Biologi dan Aplikasinya Jurnal Ilmiah Matematika Makara Journal of Science Makara Journal of Technology Annales Bogorienses Berita Biologi
WIBOWO MANGUNWARDOYO
Departemen Biologi FMIPA-UI
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Electrical & Electronics Engineering Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology

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Journal : BERITA BIOLOGI

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VARIASI GENETIK Lactobacillus fermentum Beijerink ASAL SAYUR ASIN BERDASARKAN ANALISIS RFLP 16S-23S rDNA ISR, RAPD-PCR DAN ERIC-PCR [Genetic Variation of Lactobacillus fermentum Beijerink Origin Sayur Asin Based on RFLP 16S-23S rDNA ISR, RAPD-PCR and ERIC-PCR Analysis] Sulistiani, Sulistiani; Mangunwardoyo, Wibowo; Abinawanto, Abinawanto; Sukara, Endang; Dinoto, Achmad; Salamah, Andi
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3242.558 KB) | DOI: 10.14203/beritabiologi.v16i2.2772

Abstract

Molecular analysis of Lactobacillus fermentum isolates is essential to understand their genetic variation in relations to their roles in sayur asin fermentation process. Combination of three molecular techniques which is restriction fragment length polymorphism (RFLP) of 16S23S rDNA intergenic spacer region (ISR), random amplified polymorphic DNA (RAPD-PCR) and an enterobacterial repetitive intergenic consensus (ERIC-PCR) analysis were performed to discriminate 19 representative isolates of L. fermentum isolated from sayur asin. The result showed that L. fermentum strain D11 is distantly related to other isolates based on RFLP using HhaI restriction enzyme and RAPDPCR analyses. In addition, both of RAPD-PCR and ERIC-PCR successfully determined the genetic variation among L. fermentum strains by exhibiting distinct 4-8 bands (800-2080 bp) and 4-10 bands (280-3050 bp), respectively. A dendogram generated from UPGMA cluster analysis of both RAPD-PCR and ERIC-PCR data showed two distinct genotypic groups exist among L. fermentum isolated from sayur asin in Indonesia.
ANALISIS SENYAWA BIO AKTIF DARI EKSTRAK BUI PICUNG (Pangium edule Reinw.) SEGAR Mangunwardoyo, Wibowo; Ismaini, Lily; Heruwati, Endang Sri
BERITA BIOLOGI Vol 9, No 3 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (370.188 KB) | DOI: 10.14203/beritabiologi.v9i3.781

Abstract

Pangi (Pangium edule Reinw.) seed has long been used traditionally as a preservative agent for fish, especially in remote areas.A study has been conducted on analysis of bioactive compounds of pangi fresh seeds extracts and their fractions.In this study,maceration of fresh seed using water and 50% ethanol was carried out followed by thin layer chromatography (TLC) analysis to see weather the extracts contained tannin. Both extract then separated into their respective fractions using column chromatography.Fractions which had been tested to have highest antibacterial activity were then analysed using gas chromatography mass spectrometry (GC-MS) to assess the active compounds which believe to be a preservative agent.Identification of water and 50% ethanol extract of Pangium edule Reinw.fresh seeds with TLC resulted that tannin were found in those extracts. Furthermore, GC-MS analysis showed that fractions which had been previously tested to have high antibacterial activity contained 9-octadecanoic acid whith similarity index of 89%, and 1,2-benzenedicarboxylic acid whith similarity index of 94-85%.
IDENTIFIKASI MOLEKULAR ISOLAT KAPANG PENGHASIL ß-GLUCAN BERDASARKAN DAERAH INTERNAL TRANSCRIBED SPACER (ITS) Srikandace, Yoice; Caterina A, Ines Irene; Mangunwardoyo, Wibowo
BERITA BIOLOGI Vol 9, No 5 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1088.439 KB) | DOI: 10.14203/beritabiologi.v9i5.1987

Abstract

Research was conducted to identify the fungal isolate G.23 which produces b-glucan from the Biopharmacy Laboratoriums collection.Indonesian Institute of Sciences (LIP1) based on the sequence of the Internal Transcribed Spacer (ITS) regions.DNA was isolated from mycelia and the ITS region was amplified by Polymerase Chain Reaction (PCR) with ITS1 and ITS4 primers. The PCR product was purified using the QIAquick PCR Purification kit (Qiagen). BigDye terminator cycle sequencing Ready Reaction Kit (Perkin Elmer Applied Biosystem) was used and the product was purified with the AutoSEQ G-50 Kit (Qiagen).The sequence obtained analysed using Basic Local Alignment Search Tool nucleotide (BLAST)n homology search.The BLASTn result showed that the fungal isolate G.23 belongs to the genus Aspergillus. Taxa closely related to this isolate were Aspergillus elegans,A. ochraceus and A.sclerotiorum with 96% sequence homology.ClustalX was used for sequence-alignment. Phylogenetic analysis was constructed using the Neighbour Joining (NJ) method with Kimura two parameters. The phylogenetic tree obtained showed that fungal isolate G.23 separated from A. elegans, A. ochraceus and A. sclerotiorum which indicated that fungal G.23 belonged to a different spesies.Morphological observation on culture and microscopic appearance of the fungal isolate G.23 supported that this isolate differs from A. elegans, A. ochraceus and A. sclerotiorum.
Co-Authors Abinawanto Abinawanto Achmad Dinoto Aisyah, Arina Andi Salamah ARIYANTI OETARI Atit Kanti Bustamam, Alhadi Caniago, Asnany Caterina A, Ines Irene Chandra, Nicholas Dwi Desyandri Desyandri Ekowati Chasanah Endang Sri Heruwati Endang Sukara Fitri, Reno Gintung Patantis Guswenrivo, Ikhsan I Made Sudiana Idris Idris Ifah Munifah Ikhwani, Azra Zahrah Nadirah INDRAWATI GANDJAR Indriawan, Aris Indriawan, Aris Irawan Sugoro Kafi, Rahmat Al Lily Ismaini Lusini, Yuyun Napitupulu, Toga Pangihotan Pratama, Aulia Brellian Pratitis, Asri PUSPITA LISDIYANTI Sari, Arina Findo Setiawan, Fery Hadi SHANTI RATNAKOMALA Siswodarsono, Trismilah Siswodarsono, Trismilah Sita Heris Anita Sophia, Romauli Aya Srikandace, Yoice Suhendar, Dadang Suhendar, Dadang Sukma Nuswantara Sulistiani ., Sulistiani Sulistiani Sulistiani, Sulistiani Susanti, Agustina Eko Syafriana, Vilya Trismilah Trismilah YOPI YOPI Yuliar Yuliar Yusro Nuri Fawzya