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Morphological Characteristics Selection of Acid-tolerant Leucaena leucocephala Mutant to Addition of IBA Hormone (Indole butyric acid) in Tissue Culture Panca Dewi Manuhara Karti; Mukhlisani Muhklisani; Iwan Prihantoro
Jurnal Ilmu Ternak dan Veteriner Vol 27, No 4 (2022): December 2022
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v27i4.2960

Abstract

Leucaena leucocephala is a legume forage plant that has a high protein content. Tissue culture is a technique that can be used to select mutants for forage plants in vitro. IBA (Indole butyric acid) is one type of auxin that can induce rooting and growth in plants. This study aimed to select the morphological characteristics of the acid-tolerant Leucaena leucocephala mutant to the addition of the hormone IBA in tissue culture. The design used in this study was a completely randomized design (CRD) with lamtoro plant explants as many as 11 acid-tolerant mutant lines pH 3.4 resulting from 400 gy irradiation which had been treated on 300 ppm Al3+ acid media, namely the K1-K11 strain (mutant+MS+1ppm IBA) and 2 parent trees Leucaena leucocephala without gamma irradiation, namely P0 as Leucaena leucocephala parent+MS+0ppm IBA, P1 as Leucaena leucocephala parent + MS + 1 ppm IBA with 15 replicates. The treatment which had a significant effect was continued with the test Tukey. Variables observed were an increase in root length, plant vertical height, number of shoots, and percentage of rooted plants. The increase in root length and increase in plant vertical height showed the best results on the K10 mutant strain, the number of shoots showed the best results on the K9 and K11 mutant strains, and the highest percentage of rooted plants on the K3 and K11 mutant strains. The addition of IBA can increase the morphological characteristics of the Leucaena leucocephala mutant.
Efektivitas Berbagai Produk Fungi Mikoriza Arbuskula Dalam Meningkatkan Produktivitas Stylosanthes guianensis Pada Tanah Masam: The Effectiveness of Various Arbuscular Mycorrhizal Fungi Products in Increasing Productivity of Stylosanthes guianensis on Acid Soil Agam Rizki; Panca Dewi Manu Hara Karti; I Prihantoro
Jurnal Ilmu Nutrisi dan Teknologi Pakan (Nutrition and Feed Technology Journal) Vol. 20 No. 3 (2022): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jintp.20.3.89-94

Abstract

This study aimed to evaluate the effectiveness of Arbuscular Mycorrhizal Fungi (AMF) against Stylosanthes guianensis plants on acid soils. The research was conducted in the greenhouse of the Agrostology Laboratory of IPB. The research design used was a completely randomized design with 5 treatments and 8 replications, treatments were different types of AMF, without AMF (F0), commercial AMF (FU), AMF product IPB 1 (EM1), AMF product IPB 2 (EM2) and AMF product IPB 3 (EM3). AMF was given as much as 20 grams per planting hole. The given fertilizers were manure and NPK fertilizer. The treatment without AMF was given 100% of the recommended dose, while treatment with AMF was only given 50% of the dose. The results showed that there was no significant difference between treatments on growth variables (plant height, number of trifoliate leaves, number of branches, stem diameter and leaf chlorophyll). The type of AMF affected the fresh shoot production, and the highest was at EM1 (38.03 grams), as well as the number of root infections had a significant effect, and the highest production was at EM1 (77%). The conclusion in this study was that the use of AMF could reduce the use of manure and NPK 50% from the recommended dose, could increase the growth and productivity of Stylosanthes guianensis on acid soils and AMF product IPB 1(EM1) gave the best results. Key words: root infection, marginal soil, leguminous, Stylosanthes guianensis
Tolerance Level of Butterfly Pea (Clitoria ternatea L.) to Stress Acidity Through Tissue Culture Technique Kennaya Putri Aura Jannah; Panca Dewi Manu Hara Karti; Iwan Prihantoro
Buletin Peternakan Vol 47, No 4 (2023): BULETIN PETERNAKAN VOL. 47 (4) NOVEMBER 2023
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v47i4.84290

Abstract

Butterfly pea (Clitoria ternatea L.) a high-quality legume that is rich in protein and grows on various soil types with a pH range of 5.5-8.9. This experiment was conducted to get the level of tolerance of butterfly pea plants to stress acidity at different levels through tissue culture technique. The study was designed using a complete randomized design with 6 treatments with the different levels of AlCl3 addition using Murashige Skoog (MS) media with 20 replications (P0 (0 ppm AlCl3), P1 (100 ppm AlCl3), P2 (200 ppm AlCl3), P3 (300 ppm AlCl3), P4 (400 ppm AlCl3), and P5 (500 ppm AlCl3)). Data were analyzed using analysis of variance (ANOVA), and if there was a significant difference, data were further analyzed using Duncan’s multiple range test. The variables observed were acidity media changes, plant height gain, number of leaves, number of branches, number of tillers, percentage of leaves withering, and leaf color. The results showed that the butterfly pea plant has mechanism of adaptation to acid stress on the parameters of plant height gain and number of leaves at the end of the observation. However, the level of plant tolerance on the parameters of the number of branches and the number of tillers was ≤ 300 ppm (pH 3.73).
Pengaruh Usia Potong dan Dosis Pupuk Urea Terhadap Kandungan Nutrien dan Kecernaan Sorgum Varietas Samurai 1 Sebagai Pakan Ruminansia Anwar Efendi Harahap; Luki Abdullah; Panca Dewi Manuhara Karti; Despal
Jurnal Nutrisi Ternak Tropis Vol. 7 No. 1 (2024): JNT Jurnal Nutrisi Ternak Tropis Maret
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jnt.2024.007.01.1

Abstract

Kesulitan penyediaan pakan hijauan pada pengembangan peternakan ruminansia dapat diatasi dengan penggunaan sorgum  karena  mampu menghemat biaya pakan akibat pemanenan yang dilakukan berkali kali pada satu periode penanaman. Penelitian bertujuan mengevaluasi nilai nutrien dan kecernaan sorgum samurai akibat waktu panen dan pupuk urea yang berbeda pada panen pertama. Rancangan penelitian yang digunakan yaitu RAL Pola Faktorial (3x3) dengan 4 ulangan. Faktor pertama adalah perlakuan usia potong 80, 85 dan 90 hari setelah tanam (HST) dan faktor kedua adalah perlakuan dosis pupuk urea terdiri dari 200, 300 dan 400 kg ha-1. Hasil penelitian menunjukkan terdapat interaksi (p<0.05) pada perlakuan protein kasar dan NDF, bahan kering dipengaruhi (p<0.05) usia potong serta ADF (p<0.05) dipengaruhi dosis pupuk urea. WSC dan GE tidak dipengaruhi (p>0.05) usia potong, dosis pupuk dan interaksinya. Selanjutnya KcBK dan KcBO juga tidak dipengaruhi (p>0.05) usia potong dan pemberian pupuk  Waktu panen sorgum samurai 1 usia potong 80 HST dan dosis pupuk urea 200 kg ha-1 menjadi pilihan peternak karena mampu menghasilkan pakan kaya nutrisi sebagai bahan baku pakan ternak ruminansia.
Efektivitas Fungi Mikoriza Arbuskula yang Diproduksi dengan Teknik Fortifikasi Nutrisi Berbeda terhadap Produktivitas Stylosanthes guianensis pada Cekaman Kekeringan: Effectiveness of Arbuscular Mycorrhizal Fungi Produced by Different Nutrient Fortification Techniques on the Productivity of Stylosanthes guianensis in Drought Stress Marzukah; Manuhara Karti, Panca Dewi; Prihantoro, Iwan
Jurnal Ilmu Nutrisi dan Teknologi Pakan Vol. 21 No. 2 (2023): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jintp.21.2.107-115

Abstract

This study aimed to test the effectiveness of arbuscular mycorrhizal fungi (AMF) products against stylo plants (Stylosanthes guianensis) under drought stress conditions. The research design used a factorial complete random pattern (CRD) with 2 factors. Factor A was AMF: F0 (control), FU (superior AMF as a comparison), FIPB1 (1000ppm ABmix nutrient fortification technique), FIPB2 (2000ppm ABmix nutrient fortification technique), FIPB3 (3000ppm ABmix nutrient fortification technique). Factor B was the drought level, 100% field capacity (FC), 60% FC (medium dry level), and 40% FC (high dry level). The fertilizer for control plants (without AMF) was 100% dose, while plants with AMF were given a 50% dose, adding AMF ± 20 grams. The results showed an interaction between the drought factor and the different types of AMF. AMF was effective in symbiosis with stylo plants at all levels of drought. FIPB1 and FIPB2 could increase plant adaptation up to 60% FC. All AMF inoculations effectively increased leaf chlorophyll content at all drought levels. Biomass accumulation in FU and FIPB1 plants produced the best biomass. The research concluded that the quality of AMF produced with different nutrient fortifications were as good as the superior AMF (FU). Inoculation of AMF products (IPB1, IPB2, and IPB3) with 50% fertilization effectively increased the adaptation and production of Stylosanthes guianensis to a drought level of 60% FC compared to control (without AMF, 100% fertilization). Key words: Arbuscular mycorrhizal fungi, drought level, productivity, Stylosanthes guianensis
Optimasi Level Benzyl Amino Purin (BAP) terhadap Pertumbuhan Tanaman Kembang Telang (Clitoria ternatea) melalui Teknik Kultur Jaringan: Optimization of Benzyl AminoPurin (BAP) Levels for the Growth of Butterfly Pea (Clitoria ternatea) Plants through Tissue Culture Techniques Jannah, Kennaya Putri Aura; Prihantoro, Iwan; karti, panca dewi manu hara
Jurnal Ilmu Nutrisi dan Teknologi Pakan Vol. 21 No. 2 (2023): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jintp.21.2.100-106

Abstract

The research objective was to measure the optimum level of BAP for supporting the growth of butterfly pea flowers through tissue culture techniques. The study used a completely randomized design (CRD) with 5 treatments based on BAP media levels (BAP 0 ppm, BAP 0.5 ppm, BAP 1 ppm, BAP 1.5 ppm, and BAP 2 ppm) and 20 replications. Parameters measured were plant height, plant height increase, number of leaves, number of branches, number of tillers, percentage of tiller growth, plant weight, and leaf color. The results showed that the use of BAP levels 0, 0.5, 1, 1.5, and 2 ppm in butterfly pea flower plants through tissue culture techniques was not effective in increasing plant height, number of leaves, number of branches, number of tillers, and percentage of tillers at the end of the research (27 DAP). Using an optimum BAP level of 0.5 ppm resulted in higher biomass production and the dominance of green leaf color visualization. Key words: BAP (Benzyl Amino Purine), butterfly pea, Clitoria ternatea, tissue culture
Pengaruh Pakan Berbasis Silase dengan Penambahan Minyak Sawit dan Minyak Kelapa terhadap Kecernaan pada Domba Betina: The Effect of Silage-Based Feed with Added Palm Oil and Coconut Oil on Digestibility in Ewes Anggun Cantika, Neng Risviana; Diapatri, Didid Diapari; Manu Hara Karti , Panca Dewi
Jurnal Ilmu Nutrisi dan Teknologi Pakan Vol. 22 No. 1 (2024): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jintp.22.1.50-56

Abstract

This study aimed to measure the level of nutrient digestibility in local sheep fed high-energy silage-based feed with added palm oil and coconut oil. Twelve adult ewes thathad given birth with an average body weight of 26.12 ± 3.13 kg and an average initial body condition score (BCS) of 1.7 ± 0.57 were used in this experiment. The basalt ration used was silage complete ration Sorinfer consisting of sorghum, indigofera, and concentrate. The treatments were P0= Sorinfer + 6% soybean meal (control), P1= P0 + 3% palm oil, and P2= P0 + 3% coconut oil. The variables measured included the consumption and digestibility of dry matter and nutrients. The data obtained were analyzed using ANOVA. If the results showed significant differences, further tests were performed using the Duncan test. The results showed that the addition of vegetable oil to the ration was able to increase the consumption of crude fat but decrease its digestibility. The addition of 3% coconut oil (P2) effectively increased the digestibility of organic matter, crude protein, and digestibility of Nitrogen-Free Extract (NFE) compared to controls, while rations with the addition of 3% palm oil (P1) effectively increased the digestibility of crude fiber compared to controls. The digestibility of ration nutrients in the 3% coconut oil addition treatment (P2) was more optimal than the control treatment and the addition of palm oil (P1). Key words: local ewes, nutrient consumption, nutrient digestibility, oil, silage
Karakteristik Pertumbuhan Kandidat Mutan Lamtoro (Leucaena leucocephala cv. tarramba) Generasi M1 Toleran Cekaman Masam pada Skala Lapang : Growth Characteristics of M1 Generation Candidate Mutants of Lamtoro (Leucaena leucocephala cv. tarramba) Tolerant to Acid Stress on Field Scale azwa lubis, fadhilatul; Prihantoro, Iwan; Karti, Panca Dewi Manuhara
Jurnal Ilmu Nutrisi dan Teknologi Pakan Vol. 22 No. 2 (2024): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jintp.22.2.116-121

Abstract

The selected of lamtoro var. tarramba mutant candidates tolerant to pH 3.8 are expected to grow well in a normal field-scale environment. The purpose of this study was to evaluate the growth characteristics of mutant candidates of lamtoro var. tarramba M1 generation tolerant to acid on field. This study used Randomized Block Design (RBD) of 11 mutant candidates of lamtoro var. tarramba (T1-T11) consisting of five replications. The variables measured included plant morphology (plant height, stem diameter, number of branches, and number of stalks) rhizosphere pH, and rhizosphere color. The results showed that the growth characteristics of mutant candidates of lamtoro var. tarramba M1 generation tolerant to acid pH 3.8 on field scale were equally good for parameters of plant height, stem diameter, number of branches, and number of stalks. The conclusion of the study was that the total of 11 acid-tolerant lamtoro var. tarramba mutant candidates of M1 generation were able to grow well on a field scale. All M1 generation mutant candidates produced the same good growth characteristics of plant height, stem diameter, number of branches, and number of stalks at the end of the study (8 WAP). Key words: acid tolerant, growth characteristics, lamtoro var. tarramba mutant, M1 generation
Efektifitas Hormon TDZ (Thiadiazuron) pada Multiplikasi Kultur In-Vitro Terhadap Pertumbuhan Mutan Lamtoro Varietas Tarramba (Leucaena lucochephala) Teradaptasi Asam Muhklisani, Muhklisani; Karti, Panca Dewi Manuhara; Prihantoro, Iwan
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 1 (2022): JITRO, January
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (452.138 KB) | DOI: 10.33772/jitro.v9i1.19166

Abstract

ABSTRAK Leucaena leucocephala merupakan tumbuhan pakan ternak kelompok leguminosa yang memiliki nutrisi yang baik untuk ternak. pemuliaan tumbuhan pakan ternak merupakan salah satu strategi untuk meningkatkan pemenuhan pakan  dalam segi kualitas,kuantitas dan kontinuitas. Teknik kultur jaringan merupakan langkah untuk memperoleh tanaman pakan yang yang seragam, waktu yang relatif cepat dan bibit yang dihasilkan memiliki kemampuan adaptasi terhadap berbagai cekaman dengan treatment yang diberikan pada media tanam sehingga dihasilkan bibit yang unggul. Penelitian ini bertujuan untuk mengetahui efektifitas hormon TDZ pada tumbuhan pakan ternak Leucaena leucocephala dengan teknik kultur jaringan (pembiakan in-vitro). Rancangan yang digunakan pada penelitian ini adalah RAL dengan eksplan tanaman lamtoro sebanyak 13 galur yaitu M1-M11 (mutan+MS+1ppm TDZ), K0 (kontrol+MS+0ppm TDZ), K1 (kontrol+MS+1ppm TDZ), masing masing 15 ulangan. Perlakuan yang berpengaruh nyata dilanjutkan dengan uji tukey. Variable yang diamati adalah pertambahan tinggi vertikal tanaman, diameter batang,  jumlah daun majemuk, persentase kerontokan daun dan jumlah tunas. Hasil penelitian menunjukkan pemberian hormon TDZ konsentrasi 1ppm pada media tanam Leucaena leucocephala cv. Tarramba berpengaruh nyata (p<0.05) terhadap pertumbuhan mutan. pertambahan tinggi vertikal tanaman menunjukkan hasil terbaik pada galur M9, diameter batang menunjukkan hasil terbaik pada galur M3, jumlah daun majemuk menunjukkan hasil terbaik pada galur M3, jumlah tunas menunjukkan hasil terbaik pada M9 dan persentase kerontokan daun majemuk tertinggi pada K0.Kata Kunci: TDZ, mutan, Leucaena leucocephala, kultur jaringan.The Effectiveness of TDZ (Thidiazuron) Hormone in In-Vitro Culture Multiplication on the Growth of Lamtoro (Leucaena leucocephala) Mutant Tarramba Varieties Acid AdaptedABSTRACT Leucaena leucocephala is a legume group of animal feed plants that has good nutrition for livestock. Breeding forage plants is one strategy to increase feed fulfilment in quality, quantity, and continuity. The tissue culture technique is a step to obtain uniform feed plants, relatively fast time. The resulting seeds can adapt to various stresses with the treatment given to the growing media to produce superior seeds. This study aims to determine the effectiveness of the TDZ hormone in animal feed plants Leucaena leucocephala using tissue culture techniques (in vitro culture). The design used in this study was RAL with 13 lines of lamtoro plant explants, namely M1-M11 (mutant+MS+1ppm TDZ), K0 (control+MS+0ppm TDZ), K1 (control+MS+1ppm TDZ), each 15 repetitions. The treatment that had a significant effect was continued with the Tukey test. The observed variables were the increase in vertical height of the plant, stem diameter, the number of compound leaves, the percentage of leaf loss, and the number of shoots. The results showed that gave the concentration of TDZ hormone 1 ppm to the growing media of Leucaena leucocephala cv. Tarramba had a significant effect (p<0.05) on the growth of the mutant. The increase in vertical height showed the best results on the M9 line; stem diameter showed the best results on the M3 line, the number of compound leaves showed the best results on the M3 line, the number of shoots showed the best results on M9 and the highest percentage of compound leaf loss on K0.Keywords: TDZ, mutant, Leucaena leucocephala, tissue culture
Seleksi In Vitro Mutan Alfalfa Hasil Iradiasi Sinar Gamma pada Cekaman Asam Harianja, Desima Natalia; Manuhara Karti, Panca Dewi; Prihantoro, Iwan
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 1 (2022): JITRO, January
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (623.625 KB) | DOI: 10.33772/jitro.v9i1.19445

Abstract

ABSTRAKAlfalfa  (Medicago sativa L.) telah digunakan sebagai makanan ternak yang efektif dan bergizi. Kondisi cekaman asam membatasi pertumbuhan dan produksi alfalfa dalam banyak sistem budidaya. Perbaikan genetik melalui iradiasi sinar gamma dan seleksinya dengan AlCl3+ dihasilkan keragaman genetik toleran cekaman asam. Penelitian ini bertujuan untuk mengetahui karakteristik mutan alfalfa hasil iradiasi sinar gamma yang diberi AlCl3+ sebagai simulasi cekaman asam. Rancangan yang digunakan dalam penelitian ini adalah rancangan acak lengkap (RAL) pola faktorial. Faktor pertama adalah dosis iradiasi sinar gamma yaitu 0Gy, 100Gy, 200Gy, 300Gy, 400Gy dan 500Gy. Faktor kedua adalah level AlCl3+ yaitu 100ppm, 200ppm, 300ppm, 400ppm dan 500ppm. Variabel yang diamati meliputi viabilitas, tinggi vertikal, jumlah daun, kelayuan daun, penyusutan media, bobot basah, warna daun dan perubahan pH. Hasil penelitian menunjukkan bahwa mutan alfalfa teriradiasi sinar gamma memberikan karakteristik terbaik dibanding alfalfa tanpa iradiasi sinar gamma terhadap peningkatan level AlCl3+. Hingga abang batas tertentu peningkatan dosis sinar gamma dapat meningkatkan pertumbuhan dan ketahanan alfalfa pada kondisi tercekam asam. Dapat di simpulkan bahwa induksi mutasi dengan iradiasi sinar gamma dapat dihasilkan mutan alfalfa yang tahan pada kondisi tercekam asam hingga level AlCl3+ 500ppm, dengan dosis iradiasi sinar gamma terbaik pada dosis 400Gy.Kata Kunci: alfalfa, sinar gamma, alcl3+, in vitroIn Vitro Selection of Mutants Alfalfa Result in Gamma-Ray Irradiation in Acid StressABSTRACTAlfalfa (Medicago sativa L.) has been used as an effective and nutritious animal feed. Acid stress conditions limit alfalfa growth and production in many cultivation systems. Genetic improvement through gamma-ray irradiation and its selection with AlCl3+ resulted in acid stress-tolerant genetic diversity. This study aims to determine the characteristics of alfalfa mutants resulting from gamma irradiation given AlCl3+ as a simulation of acid stress. The design used in this study was a completely randomized design (CRD) with a factorial pattern. The first factor is the gamma-ray irradiation dose, namely 0Gy, 100Gy, 200Gy, 300Gy, 400Gy, and 500Gy. The second factor is the level of AlCl3+, namely 100ppm, 200ppm, 300ppm, 400ppm and 500ppm. The variables observed included viability, vertical height, number of leaves, leaf wilting, media shrinkage, wet weight, leaf color, and changes in pH. The results showed that the alfalfa mutant irradiated with gamma rays gave the best characteristics compared to alfalfa without gamma irradiation to increase the level of AlCl3+. Up to a certain threshold, increasing the dose of gamma rays can increase the growth and resistance of alfalfa under acid stress conditions. It can be concluded that mutation induction with gamma-ray irradiation can produce alfalfa mutants that are resistant to acid stressKeywords: alfalfa, gamma rays, alcl3+, invitro