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All Journal Jurnal Penelitian Saintek Jurnal Ilmu Kimia Universitas Brawijaya Natural B The Journal of Pure and Applied Chemistry Research Science and Technology Indonesia ALCHEMY Jurnal Penelitian Kimia Chemistry Indonesian Journal of Chemical Research JKPK (Jurnal Kimia dan Pendidikan Kimia) Jurnal Ilmiah Berkala: Sains dan Terapan Kimia Molekul: Jurnal Ilmiah Kimia

Diah Mardiana

Department Of Chemistry, Faculty Of Mathematics And Natural Sciences, Universitas Brawijaya, Jl Veteran Malang 65145, Jawa Timur, Indonesia

Author-ID : 315068

Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Energy Engineering Environmental Science Materials Science & Nanotechnology Medicine & Pharmacology Physics

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Early Poly Mass Effect (trimethylene-sebasat) On Biodegradation Rate In Liquid Media Using Mucor Miehei In Aerobic Kholisul Fuad, Akhmad; Mardiana, Diah; Roosdiana, Anna
Natural B, Journal of Health and Environmental Sciences Vol 1, No 3 (2012)
Publisher : Natural B, Journal of Health and Environmental Sciences

The author has conducted research about the effect of poly(trimethylene-sebacate) mass toward the rate of biodegradation using Mucor miehei in aerobic liquid media. Poly(trimethylene-sebacate) is a biodegradable linear aliphatic polyester, that can be degraded by Mucor miehei lipase. To determine the effect of poly(trimethylene-sebacate) mass in the biodegradation, the mass of poly(trimethylene-sebacate) were varied 0.06 g, 0.08 g, 0.1 g, 0.12 g and 0.14 g. Biodegradation process carried out for 12 hours, using liquid of Complex media, which was nutrient rich for Mucor miehei growth, and solution at pH 5. The resulting CO2 gas was flowed into the 50 mL reservoir of 0.1 M NaOH, followed by titration using 0.05 M HCl and MO (methyl orange) and PP (phenolptalein) indicator. The rate of CO2 gas were 0.287 x 10-3 M/h; 0.102 x 10-3 M/h; 0.137 x 10-3 M/h; 0.016 x 10-3 M/h; and 0.039 x 10-3 M/h respectively. The greater rate of CO2 produced the lower mass of poly(trimethylene-sebacate).

Adsorption of Mg (II) Ion Using Chlorophilized Chitin Adsorbent Darjito Darjito; Rachmat Triandi; Diah Mardiana; M. Fathoni
Natural B, Journal of Health and Environmental Sciences Vol 2, No 1 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

A study concerning the adsorption of Mg (II) ions using phosporilation chitin adsorbent. The process is a surface modification of adsorbent chitin by enriching the phosphate group that acts as a hard base so it would be more suitable binding Mg (II) ions, which acts as a hard acid. This research aims to study the effect of contact time and concentration variations of Mg (II) ions on the adsorption process using phosporilation chitin adsorbent. Variables to be examined in this study in order are contact times (40, 60, 80, 100, and 120 minutes) and the concentration of Mg (II) ions variation are (10, 25, 50, 75, 100, 125, 150, 175, and 200 ppm). The results for the characterization of phosporilation chitin adsorbent can be detect from the IR absorption band at wavenumber 1099.35 cm-1 are suspected of phosphate ions and IR absorption band at wave number 1272.93 cm-1 and 1201.57 cm-1 are estimated as phosphate covalent. The contacts times significantly affect the adsorption Mg (II) ions by the phosporilation chitin adsorbent, then the optimum of contact time is 120 minutes. Adsorption capacity of phosporilation chitin adsorbent for the Mg(II) ions is 499 mg/g.

Effect of Adding Ions - Metal Ions to Pectinase Activity Isolation from Bacillus Subtilis on Bleaching Paper Mardiana Prasetyani Putri; Anna Roosdiana; Sasangka Prasetyawan; Diah Mardiana
Natural B, Journal of Health and Environmental Sciences Vol 2, No 1 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

Pectinase enzyme used as biocatalyst to overhaul pectat compounds or pectin. Pectinase avtivity was affected by metal ions. The purpose of this study is to determine the effect of adding metal ions on pectinase activity and its inhibition type.. Determination of enzyme activity was based on galacturonic acid produced, which was analyzed by spectrophotometric using DNS reagent. Concentration of metal ions used i.e. Zn2+, Cu2+, Fe3+ were 2-10 mM. The result showed that Vm and KM number without ions were 161.29 µmol/mL minutes and 0.55%(w/v), respectively. While the presence of ions Zn2+, Cu2+, Fe3+ were producing Vmapp 147.06; 156.25 and 153.85µg.ml-1min-1, KMapp of 0.56%; 0.63% and 0.65%, and also KI of 333,33; 41,38; 32,9. The inhibition to metal ions are non-competitif inhibition.

Effect of Metal Ions on Pectinase Activity from Aspergillus niger on Purifying Guava Fruit Dian Puspita Anggraini; Anna Roosdiana; Sasangka Prasetyawan; Diah Mardiana
Natural B, Journal of Health and Environmental Sciences Vol 2, No 1 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

Pectinase can be used on various fruit juices clarification; however, the activities can be affected by their metal ions content. The purpose of this study was to determine the type of metal ions inhibition i.e. Zn2+; Mg2+ and Ca2+ on the pectinase activity of Aspergillus niger, and its potential application in guava juice clarification. Activity assays performed at pH 5, temperature 50°C during 55minutes and each ion concentration were 2-10 mM. Galacturonic acid concentration, as the product of pectin substrate hydrolysis, used as the basis for determining its activity and analyzed by visible spectrophotometry. The results showed that the ions concentration of Zn2+ and Mg2+ more than 4 mM were inhibitor for pectinase activity while Ca2+ ion concentration was 6mM. KI values for the metal ions Zn2+; Mg2+ and Ca2+ at concentrations of 6mM were 176.99; 326.09 and 280.37 mM respectively. Pectinase from Aspergillus niger were able to reduce 18% of pectin of guava juice.

Co-Authors Agung Yanuar Pratama Akhmad Kholisul Fuad Ani Mulyasuryani Anna Roosdiana Anna Roosdiana Arie Srihardyastutie Ayunda Arum Sari Bambang Poerwadi Barlah Rumhayati Budi Kamulyan Budi Kamulyan Darjito Darjito Dewi Umaningrum Dian Puspita Anggraini Dwi Siswanta El Fajriyah Aulia Putri Ellya Indahyanti Elvanila Tanjung Fetty Alvionita Sitanggang Hasanuddin Hasanuddin Jumina Jumina Khoirul Ngibad Layta Dinira Lukman Hakim M. Fathoni Mardhatillah Mardhatillah Mardiana Prasetyani Putri Maria Dewi Astuti Maria L.A.D Lestari Maria Lucia A.D Lestari Maria Lucia Ardhani Dwi Lestari Rachmat Triandi Radna Nurmasari Riawati Riawati Ridho Arief Al Rasyid Ridho Firmansyah Sasangka Prasetyawan Sepriana Puspita Dewi Syaiful Siannita Chandra Syaifudin, Achmad Ulfa Andayani Ulfa Andayani, Ulfa Urnik Rokhiyah Usyqi Salsabila Zubaidah Ningsih Zubaidah Ningsih Zubaidah Ningsih Zubaidah Ningsih

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