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Analisis Molekuler Bakteri Endofit SH 14 dari Mangrove Scyphiphora hydrophyllacea Pantai Rendani Yamco, Fallen; Massora, Maria; A. Mogea, Rina
Cassowary Vol 7 No 2 (2024): Juni
Publisher : Program Pascasarjana Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30862/casssowary.cs.v7.i2.284

Abstract

ABSTRACT: The mangrove endophytic bacterial isolate Scyphiphora hydrophyllacea has antimicrobial capabilities and also has other bioactive compounds to be developed in the field of biotechnology. The aim of this study was to identify the endophytic bacterial isolate SH 14 obtained from the S. hydrophyllacea mangrove based on 16S rDNA sequence analysis. Molecular-based identification of bacteria, namely DNA amplified by Polymerase Chain Reaction (PCR) using primers 27F and 1492R and has a gene amplification of 1481 bp. Sequence data analyzed using the BLAST program showed that isolate SH 14 had a homology level of 99.43% with Brevibacillus brevis. This similarity value is a reference in determining bacterial identification, where bacteria are categorized as the same species if they have a similarity of 99%.
Identifikasi molekuler bakteri indigenous penghasil enzim amilase dari pati sagu asal Poom Kepulauan Yapen Hesron; Massora, Maria; A. Mogea, Rina; Wanggai. I. M; Utami. P
Cassowary Vol 8 No 3 (2025): Juli
Publisher : Program Pascasarjana Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30862/casssowary.cs.v8.i3.320

Abstract

ABSTRACT: This study aimed to identify indigenous bacterial isolates producing Amylase ezyme from sago starch from Poom, Yapen Islands, using the 16S rDNA sequence molecular method. The isolates identified were IA4 and IA8, which exhibited the highest values of assimilase enzyme activity. Bacteria were identified based on DNA amplification using Polymerase Chain Reaction (PCR) with primers 27F and 1492R. Based on the results of molecular identification, the similarity index of Isolate IA4 and Bacillus cereus D21 is 97.80%, while isolate IA8 had a similarity index of 99.79% with Alcaligenes faecalis Y5. Therefore, the amylolytic bacterial isolates were named Bacillus cereus strain IA4 and Alcaligenes faecalis strain IA8.
Morphology of Lichen Fungi in Amban Regency, Manokwari, West Papua Nebore, Idola Dian Yoku; Lefaan, Paskalina Th.; Massora, Maria; Nunaki, Jan H.; Dewi, Resmila; Fatem, Sepus Marten
Jurnal Biologi Tropis Vol. 25 No. 4 (2025): Oktober-Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4.9973

Abstract

Lichens are symbiotic association between fungi (mycobionts) and photosynthetic partners (phycobiont) that play essential ecological roles as bioindicators, pioneers in succession, and contributors to nutrient cycling. Despite their importance, the diversity and morphology of lichens in West Papua remain poorly documented. This study aimed to assess the morphology diversity of lichen taxa in Amban Regency, Manokwari, West Papua. The method used was explorative surveys with purposive sampling in Amban Regency (site I, II, and III) based on low, moderate, and high air quality. Lichen identification was based on morphological characteristic. The results showed that there were 27 species lichens belong to 12 genera and 8 families Chrysothrichaceae, Coenogoniaceae, Collemataceae, Graphidaceae, Megalosporaceae, Parmeliaceae, Physciaceae, and Strigulacea. The dominant species were from the families of Graphidaceae. Crustose lichens dominated (74%), foliose lichens (18%), with filamentous and leprose (4% each), while fruticose types were absent. Crustose lichens exhibited strong substrate adhesion and tolerance to variable microclimatic conditions with temperatures between 26,1-29,3°C, whereas foliose and filamentous forms were confined to more stable, and humid habitats between 72,0-85,4%. Continued surveys are recommended to monitor potential shifts in community composition under climate change and habitat disturbance.
Pengenalan Satwa Papua dan Status Konservasinya melalui Sosialisasi di Sekolah SMA Bintuni Papua Barat: Introduction to Papuan Animals and Their Conservation Status through Socialization at Bintuni High School, West Papua Kawulur, Elda Irma Jeanne Joice; Massora, Maria; Sutarno, Simon; Lefaan, Paskalina Theresia; Panjaitan, Rawati; Salosa, Yenni; Ratnawati, Sita; Budirianto, Heru Joko
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 9 No. 9 (2024): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v9i9.6849

Abstract

Papua has a high level of species diversity and endemism. This community service activity (PKM) is motivated by the decreasing of Papua's biodiversity due to anthropogenic activities and global warming, so it is necessary to make efforts to increase public awareness, especially among the children about the concept of protection, management, and use wisely and sustainably for Papua's diversity. Therefore, we carried out socialization about the Papuan animals and their conservation status to students at SMA Negeri 1 and SMA YPPK St. Arnoldus Jansen in Bintuni Bay District, West Papua Province on 10-11 December 2021. Socialization activities involve lectures, questions, and answers, filling out questionnaires, and giving rewards. The results showed that 61.54% of participants did not know the conservation status of animals in Papua based on Indonesian and international regulations. In addition, as many as 87.18% of participants stated that some animals in Papua were sold in the market and participants also consumed some of these animals. However, the participants' desire to protect Papua's animals is quite high (94.87%).
Keefektivan Bio-enzim Asal Limbah Buah dan Campuran Limbah Buah dengan Rumen Ayam Sebagai Aktivator Pengkomposan Mardiyono, Mohammad Sholeh; Nugroho, Julius Dwi; Massora, Maria
Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Vol 14 No 2 (2024): Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (JPSL)
Publisher : Pusat Penelitian Lingkungan Hidup, IPB (PPLH-IPB) dan Program Studi Pengelolaan Sumberdaya Alam dan Lingkungan, IPB (PS. PSL, SPs. IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jpsl.14.2.288

Abstract

Bio-enzim dapat dihasilkan dari proses fermentasi libah buah. Bio-enzim tersebut dapat bekerja sebagai aktivator pengkomposan bahan organic. Dalam penelitian ini, activator pengkomposan asal limbah buah, limbah buah yang ditambah dengan rumen ayam dan Effective Microorganism-4 (EM-4) dibandingkan dan dievaluasi keefektivannya untuk produksi kompos. Hasil penelitian menunjukkan bahwa puncak suhu dari ketiga activator tersebut terjadi pada hari ke 3 dan bervariasi antara 55-57oC. Suhu menurun pada hari ke 14 dengan suhu yang sama 33oC, yang menunjukkan bahwa kompos telah mencapai tingkat kematangannya pada waktu yang sama.. Berdasarkan hasil analisis statistic, perbedaan penggunaan ketiga activator pengkomposan dijumpai pada C-organik dan kadar air kompos. Walaupun demikian, kompos yang dihasilkan berdasarkan Keputusan Menteri Pertanian Republik Indonesia No. 261/KPTS/SR.310/6.M/4/ 2019 dan SNI 19-7030-2004 kompos yang dihasilkan sama baiknya dan memenuhi standar yang ditentukan. Dengan demikian dapat disimpulkan bahwa activator pengkomposan terbuat dari limbah buah, baik yang tanpa maupun yang ditambahkan dengan rumen ayam sama efektifnya dengan aktivator pengkomposan EM-4.
Soil analysis of Rhizospher Mangrove Rhizophora Sp East Bintuni Village Sombo, Debora; Mogea, Rina Anita; Massora, Maria
Indonesian Journal of Multidisciplinary Science Vol. 3 No. 11 (2024): Indonesian Journal of Multidisciplinary Science
Publisher : International Journal Labs

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55324/ijoms.v3i11.974

Abstract

This study aims to examine the physical and chemical conditions of the soil in the rhizosphere of Rhizophora sp. mangroves in East Bintuni Village. The soil samples were collected from two locations, namely, the Rhizopora mangrove around the central market and the mangrove forest on Jalan Nusantara. The results of the laboratory analysis showed that the soil of the Mangrove Mangrove Around the Central Market has a soil texture of 3.75%; dust 54.80%; clay 41.45% so that based on the soil texture triangle by the United States Department of Agriculture (USDA), it belongs to the Silty clay class. The C-organic value of mangrove soil around the market was 5.90% and the archipelago mangrove forests were 5.12% in the very high category. The pH value of the Rhisphere mangrove is 2.95, and the pH value is 3.82, both locations of the pH criteria were very acidic. The Al and Fe values of the RH mangrove are very high (22.86 cmol/kg) and the C-Organic value is very low (39.6 and 41.1, respectively) while the CEC value is medium (18.8 and 41.2, respectively). The results could inform conservation and management practices, helping to preserve and restore mangrove habitats in the region and potentially guiding similar efforts in other coastal areas. In addition, research could examine the long-term effects of these soil conditions on mangrove ecosystems' ability to provide critical ecological services, such as carbon sequestration and coastal protection.
Karakterisasi dan Identifikasi Senyawa Metabolit Sekunder dari Bakteri yang Bersimbion dengan Ascidia gemmata Rumalolas, Nuryanti; Mogea, Rina Anita; Massora, Maria
Jurnal Penelitian Pendidikan IPA Vol 9 No 8 (2023): August
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i8.3874

Abstract

Isolation of secondary metabolites from bacteria that have a symbiont with Ascidians has been shown to have enormous potential. This study aims to identify the types of secondary metabolites in Bacillus sp. which has a symbiont with A. gemmata. The research was carried out using laboratory experimental methods by means of phytochemical screening. After testing, the results of the characterization of bacteria that lead to the genus Bacillus and the secondary metabolites contained are alkaloid compounds