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All Journal Biotika: Jurnal Ilmiah Biologi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biogenesis: Jurnal Ilmiah Biologi Prosiding Seminar Biologi Biology, Medicine, & Natural Product Chemistry Edumatsains Jurnal Biodjati Jurnal Biologi Tropis Biosaintifika: Journal of Biology & Biology Education Jurnal Pembelajaran dan Biologi Nukleus Jurnal Pembelajaran Pemberdayaan Masyarakat (JP2M) Sciscitatio : Journal for Biological Science Jurnal Pengabdian kepada Masyarakat Nusantara Jurnal Ketenagakerjaan JURNAL BIOLOGI PAPUA SERVIRISMA: Jurnal Pengabdian kepada Masyarakat Pelita: Jurnal Pengabdian kepada Masyarakat
Ratih Restiani
Program Studi Biologi, Fakultas Bioteknologi, Universitas Kristen Duta Wacana
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Other

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Effect of BAP and 2,4-D Combination on the Callus Induction of Robusta Coffee (Coffea canephora Pierre ex A.Froehner) Leaf Explants Yovita Angeline Wijaya; Ratih Restiani; Aniek Prasetyaningsih
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 4: Jurnal Pembelajaran Dan Biologi Nukleus December 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i4.8338

Abstract

Background: Sustainable production of Robusta coffee (Coffea canephora) is constrained by the limited availability of high-quality planting materials, while information regarding the optimal auxin–cytokinin combinations for initiating callus cultures remains insufficient. Accordingly, this study was conducted to examine the effects of combined applications of benzyl amino purine (BAP) and 2,4-dichlorophenoxyacetic acid (2,4-D) on callus induction and subsequent growth derived from young leaf explants of Robusta coffee. Methodology: Explant cultures were established on Murashige and Skoog (MS) medium enriched with different concentrations of 2,4-D (1, 1.5, and 2 ppm) in combination with BAP (1.5, 2, and 2.5 ppm), arranged in a completely randomized design with three replications. Data collection was conducted throughout a 30-day incubation period and included measurements of callus initiation time (days), callus formation percentage, callus growth intensity, and callus morphological features. The resulting data were analyzed statistically using analysis of variance (ANOVA), followed by the Kruskal–Wallis test. Findings: Result describe that the treatment combining 2,4-D at 1 ppm with BAP at 1.5 ppm produced the most favorable response, as indicated by the shortest callus initiation period (7.33 DAP). In contrast, the application of 2,4-D at 1 ppm in combination with BAP at 2.5 ppm achieved a 100% callus induction rate and the highest callus growth intensity (2 on a 5-point scale), along with a compact callus exhibiting a yellowish-green coloration. Overall, these results highlight the critical influence of auxin–cytokinin equilibrium in enhancing callus induction in Robusta coffee and underscore its significance as a foundational reference for establishing an efficient in vitro propagation strategy. Contribution: In general, this study contributes to providing important technical information supporting the large-scale and sustainable production of high-quality Robusta coffee seedlings
Co-Authors Adityarini, Dwi Agustinus, Vania Alba Wijaya Maharani Anggel Christia Dolonseda Aniek Prasetyaningsih Aniek Prasetyaningsih Aniek Prasetyaningsih Ariestanti, Catarina Astrid Helena Ayu Sekar, Astrid Christini Bernaindah Nadapdap Christy, Nita Elvira Cindy Talenta Hutabarat Dena, Amelia Devi Sonti Sibarani Dolonseda, Anggel Christia Dwi Aditiyarini Dwi Adityarini Ekaria Evieyana Evieyana Galgani, Gemma Gardha Viorenta, Miranda Haryono, Sevien Elamaria Heralius Dwiki Anggoro Josiah Herald Matheos Kaban, Sarah M.P. Kaban, Sarah Mega Pratenna Kendek Marendeng, Yunita Kristiawan, Yoga Angkawijaya Marendeng, Yunita Kendek Matheos, Josiah H. Matheos, Josiah Harold Matthew Linardi Meyga Semarayani, Cokorda Istri Miranda Gardha Viorenta Nathania, Yohana Elsa Nugroho, Wahyu Setyo Pono, Putri Purba, Laurentia Henrieta Permita Sari Putri Octaviana Ginting, Naomi Bastanta Rizki Wijaya Rolas Sinambela, Gracia Sekar, Astrid A. Sekar, Astrid Ayu Sibarani, Devi Sonti Sinaga, Meisi Olivia Sinambela, Gracia Rolas Viorenta, Miranda Gardha Yovita Angeline Wijaya