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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences The Journal of Experimental Life Sciences (JELS) Biotropika BIOWALLACEA Research Journal of Life Science Medfarm: Jurnal Farmasi dan Kesehatan

Retno Mastuti

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Author-ID : 317682

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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Microsatellite Marker for Genetic Variation Analysis in Local Chili Pepper (Capsicum frutescens L.) Induced by Ethyl Methane Sulfonate (EMS) Ria Reinnata Juliandari; Retno Mastuti; Estri Laras Arumningtyas
Journal of Tropical Life Science Vol. 9 No. 2 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.09.02.08

Mutation using Ethyl Methane Sulfonate (EMS) is a simple and quick method to produce genetic variation in chili pepper. In this study, a total of 3 genotypes of local chili pepper (Capsicum frutescens L.), i.e. Genotype 2 (G2), Genotype 7 (G7), and Genotype 11 (G11) were induced by EMS with concentrations of 0% (K0), 0.01% (K1), 0.02% (K2), and 0.04% (K3). Genetic variation analysis in mutant was performed based on 3 microsatellite markers CA 19, CA 27, CA 62. Those molecular markers successfully detected the genetic variation in chili pepper mutant based on the number and size of microsatellite alleles variation. The 3 genotypes of chili pepper mutant produced a total of 15 alleles with the average Polymorphism Information Content (PIC) value of 0.82. Compared to the control plant, genetic variations in genome level were observed in local chili pepper. Furthermore, the treatment of EMS with concentration of 0.04% produced the most notable genetic variation in 3 genotypes of local chili pepper.Â

Assessing Exogenous L-Glutamine and PEG Influence on Soybean Adventitious Roots Culture: Lateral Root Morphology and Amino Acid Profiles: L-Glutamine and PEG Influence on Adventitious Roots Culture Ardhian, Dhike; Mastuti, Retno; Nahampun, Hartinio Natalia
Journal of Tropical Life Science Vol. 15 No. 1 (2025)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.15.01.01

Soybean (Glycine max L. Merr.) plays an important role in global agriculture and provides a primary source of plant nutrition, notably amino acids. However, tropical climates often result in lower amino acid levels than subtropical climates. This study explored the effects of L-glutamine and PEG on enhancing the amino acid content in in vitro soybean adventitious root. In this study, in vitro adventitious roots from the Detap1 soybean variety were treated in a liquid medium with various concentrations of L-glutamine (0, 1, 2, 4, 8, and 16 g.L-1) or PEG (0, 10, 20, 40, 80, and 160 g.L-1) for 8 days. The root biomass was quantified, and the amino acid profile was analyzed using HPLC. L-glutamine or PEG treatments significantly reduced root biomass by inhibiting lateral root initiation. Meanwhile, 20 amino acids were detected in the control and L-glutamine or PEG treatments. The application of a 16 g.L-1 L-glutamine significantly increased amino acid content. PEG treatments also had similar results in root growth inhibition and increased amino acid accumulation. Multivariate analysis showed significant separation between the control and treatment groups, with glutamine playing a crucial role in the group separation. This study demonstrates the potential of in vitro technology to improve amino acid production through adventitious root culture, providing insights into stress responses and metabolic regulations

The Relationship of Chemical Compounds and Crystal Development in Porang Tuber (Amorphophallus muelleri Blume) Chairiyah, Nurul; Harijati, Nunung; Mastuti, Retno
The Journal of Experimental Life Science Vol. 13 No. 1 (2023)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2023.013.01.01

Chemical compounds are presumed to interact with each other and potentially affect the crystal development of porang tubers. This study focused on glucomannan and calcium oxalate (CaOx) as chemical compounds found in porang. Crystal development was analyzed by microscopic observation. The tuber tissue was used for microscopic slides and was harvested two weeks before the plants lost their leaves, when the plants lost their leaves, and two weeks after the plants lost their leaves. The CaOx and glucomannan content was measured using a modified extraction method. The increasing of CaOx content tends to increase the number of CaOx crystals. The analysis showed that the crystal density could affect the increasing of tuber weight possibility. There was a development process in raphide crystals that showed by different sizes at three harvest times, which the others were not. The results also indicated that glucomannan might influence the crystal density and the CaOx content. It can be concluded that glucomannan can potentially induce CaOx synthesis and crystal count increase. Keywords: Caox, Crystal, Development, Glucomannan, Interact, Porang.

OPTIMIZATION OF GREEN SYNTHESIS OF GOLD NANOPARTICLES USING CHILI FRUIT EXTRACTS (Capsicum Sp.) Oktavia, Anggraeni In; Mastuti, Retno; Rosyidah, A’liyatur; Arumingtyas, Estri Laras
MEDFARM: Jurnal Farmasi dan Kesehatan Vol 14 No 1 (2025): Medfarm: Jurnal Farmasi dan Kesehatan
Publisher : LPPM Akafarma Sunan Giri Ponorogo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.48191/medfarm.v14i1.502

The use of plant extracts as reductants in green synthesis of gold metal nanoparticles (AuNPs) requires optimal conditions of temperature, extract concentration and pH. The objective of this research is to identify the optimal synthesis conditions for producing gold nanoparticles (AuNPs). The reducing agents employed are extracts from three varieties of chili peppers: Katokkon, Cakra Hijau, and Paprika Merah. Variations of plant extract concentrations of 10%, 15%, and 20%, incubation temperatures of 25°C, 37.5°C, and 50°C, and pH levels of 5, 7, and 9. The optimization formula design was determined using Box-Behnken, Design Expert version 13.0. The formation of AuNPs was indicated by a change in the color of the solution from yellow to pink to purple. Surface plasmon resonance was observed in the range of 200-800 nm using a microplate reader. Chili, Katokkon, Cakra Hijau and Red Paprika extracts were able to synthesize Au into AuNPs. The optimal formula for synthesizing AuNPs using Katokkon and Red Paprika extracts at a concentration of 10% while Ckra Hijau at a concentration of 15%. The incubation temperature used was 37.5°C for Katokkon extract and 25°C for Cakra Hijau and Red Pepper extract. The pH level used for the synthesis of AuNPs was pH 5 for all chili pepper extracts.

Growth Response of Purple Corn (Zea mays var. Ceratina kulesh) to Endophytic Bacterial Biofertilizer Treatment Novelia, Kiki Riska; Mastuti, Retno; Rahajeng, Saptini Mukti
Biotropika: Journal of Tropical Biology Vol. 10 No. 2 (2022)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2022.010.02.02

Jagung ungu merupakan salah satu komoditas jagung di Indonesia dengan kandungan antosianin yang tinggi sehingga baik bagi kesehatan. Upaya peningkatan hasil komoditas ini dilakukan dengan menggunakan pupuk hayati bakteri endofit yang banyak berpengaruh positif terhadap pertumbuhan serta kandungan metabolit sekunder pada tanaman. Penggunaan pupuk endofit terbukti mampu meningkatkan pertumbuhan dan hasil panen jagung kuning. Oleh karena itu penelitian ini bertujuan untuk mengetahui respon pertumbuhan tanaman jagung ungu terhadap pupuk hayati bakteri endofit. Penelitian ini menggunakan rancangan acak lengkap dengan dua faktor perlakuan yaitu pupuk hayati bakteri endofit dengan dosis 0 ml/l (kontrol, P0), 80 ml/l (P1), dan 90 ml/l (P2) dan umur tanaman yaitu 21, 35, 49, dan 63 HST. Pengaruh kedua faktor diamati pada pertumbuhan vegetatif sedangkan pada pertumbuhan generatif dan pasca panenhanya diamati pengaruh dosis pupuk. Parameter pertumbuhan vegetatif yang diamati meliputi tinggi tanaman, jumlah daun, panjang dan lebar daun. Parameter pertumbuhan generatif dan pasca panen yang diamati adalah umur berbunga, jumlah tongkol per tanaman, pajang tongkol, bobot basah dan kering tongkol serta bobot 100 biji jagung ungu. Hasil penelitian menunjukkan bahwa dosis pemupukan dan umur tanaman berpengaruh nyata terhadap tinggi tanaman, serta jumlah, panjang dan lebar daun. Dosis pemupukan berpengaruh yang nyata terhadap waktu berbunga, panjang tongkol, bobot basah dan bobot kering tongkol jagung ungu, namun belum memberikan pengaruh yang nyata terhadap bobot 100 biji jagung.

Effect of Bulbils Position on Leaf Branches to Plant Growth Responses and Corms Quality of Amorphophallus muelleri Blume Mastuti, Retno; Harijati, Nunung; Arumingtyas, Estri Laras; Widoretno, Wahyu
The Journal of Experimental Life Science Vol. 8 No. 1 (2018)
Publisher : Graduate School, Universitas Brawijaya

Bulbil (corm leaf) is one material source of vegetative propagation in Amorphophallus muelleri Blume. Based on the position in branches there are two types of bulbil, middle and edge bulbils, which are different in shape and size. It has been reported that bulbil size affected not only the growth response of seedlings and plants but also the quality of the produced corms. Therefore, the objectives of this experiment were to study the effect of bulbils origin on A. muelleri Blume (Porang) growth and the quality of harvested corms. The quality of corms was assessed based on glucomannan and (CaOx) content. Bulbils from the middle and edge of branches were grown in polybag (f 5 cm) containing compost:fertilizer (1:1) mixed media under 40% shade. Each bulbil origin was repeated 8 times. The results showed that growth responses of plant from middle bulbil were significantly better than that from edge bulbil. However, at the harvesting time, the weight and diameter of corms derived from both types of bulbils were not significantly different. The glucomannan and calcium oxalate (CaOx) content of harvested corms grown from middle bulbil tends to be higher than that from edge bulbil.Keywords: branching, calcium oxalate (CaOx), germination, glucomannan, Porang.

In Vitro Morphogenesis Responses of Various Explant in Physalis angulata L. Mastuti, Retno; Munawarti, Aminatun
The Journal of Experimental Life Science Vol. 7 No. 2 (2017)
Publisher : Graduate School, Universitas Brawijaya

The objective of this research was to identify morphogenesis responses of in vivo and in vitro-derived explant of Physalis angulata L. Explants were cultured on MS medium containing 3% sucrose solidified with 1.3% agar supplemented with combination of 6-benzylaminopurine (BAP), indole acetic acids (IAA), naphthalene acetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D). All explant types have morphogenesis ability. Capability for shoot regeneration was observed in high number of shoot tip (96.1%) and nodes explants (75.2%). Callus formation was dominantly produced by in vitro leaf explants. In vivo leaf had low responses for shoot/root regeneration and callus formation. These results provided an alternative choice of the explant suitable for either plant regeneration or callus formation.Keywords: callus, cotyledonary nodes, explant types, shoot regeneration.

Effect of Banana Homogenate on Shoot Regeneration of Ciplukan (Physalis angulata L.) Apensa, Varni; Mastuti, Retno
The Journal of Experimental Life Science Vol. 8 No. 1 (2018)
Publisher : Graduate School, Universitas Brawijaya

In vitro culture techniques can be used as an alternative problem solving in an effort to provide Ciplukan plant as a raw material of herbal medicine in a sustainable manner. The purpose of this study was to observe the effect of banana homogenate (BH) added into culture medium on shoot regeneration of Ciplukan (Physalis angulata L.) and plantlet development until acclimatization stage. The experimental design used was a randomized factorial group. The first factor was the two varieties of banana fruit namely, Ambon and Raja. The second factor was concentration of BH with four levels, 0 (control), 2.5%, 5% and 7.5 % and each treatment combination was repeated five times. Explant used in this research was the third node of 2 weeks old in vitro shoot after subculture to MS0 medium. Data analysis used ANOVA test. Eight week after culture (WAC) medium containing BH induced higher number of shoot compare to medium without BH. The addition of 5% BH var. Ambon had significant effect on increasing shoot number. Well rooted plantlets produced in MS0 rooting medium also showed high survival rate (66.7%< - 100%) two weeks after transferred to greenhouse. Therefore, the addition of BH into in vitro medium can be an alternative method for micropropagation of Ciplukan.Keywords: Banana homogenate, Ciplukan, micropropagation, nodal explants.

Anatomical Characters of Shoot Apical Meristem (SAM) on Bulbil Porang (Amorphophallus muelleri Blume) At the End of Dormancy Period Afifi, Muhammad Naufal; Harijati, Nunung; Mastuti, Retno
The Journal of Experimental Life Science Vol. 9 No. 1 (2019)
Publisher : Graduate School, Universitas Brawijaya

Porang is one species member of the genus Amorphophallus. In Indonesia, Porang is cultivated extensively because it has a high economic value. There is three seeded material available for planting. One of seeded material is bulbil. Bulbil is a vegetative reproductive organ of Porang plants which is located in the branch of the leaf. Bulbil has 3 - 4 months dormancy period. The dormancy period will end with shoot growth. Morphologically, there can be found a rough structure in the form of bulges in bulbil. The bulge is spread throughout the bulbil surface and is a growing area of shoots. Shoots on bulbil will grow through the bulge and only grow on the adaxial part, contrary shoots do not grow from the abaxial part. This study aims to compare the anatomy of shoot apical meristem (SAM) in the adaxial and abaxial parts of bulbil at the end of the dormancy period, ie 100 days after harvest (DAH), 110 DAH, 120 DAH, and 130 DAH. Anatomical observations were carried out by making semi-permanent preparations. The results showed that the anatomy of the adaxial and abaxial parts of bulbil Porang had differences in the development of SAM, leaf primordia, primary thickening meristem (PTM) and procambium.Keywords: Anatomy, Bulbil Porang, Dormancy, Shoot Apical Meristem.

Effect of Place and Time Storage on the Quality of Tubers Amorphophallus muelleri Blume Al Adawiah, Putri Rabiah; Azrianingsih, Rodiyati; Mastuti, Retno
The Journal of Experimental Life Science Vol. 9 No. 1 (2019)
Publisher : Graduate School, Universitas Brawijaya

Porang tubers are plant commodities that physiologically susceptible to damage. Longtime storage can decrease the quality of tubers. Furthermore, the presence of fungi or bacteria makes tubers cannot be stored in a long time. Decreasing quality of Porang tuber became a problem because of the higher demand for porang tubers. This research was conducted to determine the suitable place and time storage of porang tubers. Therefore, farmers can control or reduce the decrease in tuber quality. Methods of this research were an observation of color change based on the Munsell Color System, odor observation, hardness observation using penetrometer, the rotten area of tuber was measured using Leaf Area Meter, shrinkage of tuber weight, the percentage of rotten and sprouted tubers, density, and moisture content by the oven method. The results of this study were analysed statistically by ANOVA test. The hardness of the tubers has a significant effect on the storage place (soil, rack, and floor) for period 14 weeks. Storage time has an effect on changes of the weight loss, moisture content and decay area. The longer the tuber stored the higher percentage of sprouted and rotten tubers. The color of porang tubers change and the odor in the part of the rotten tubers increase during the storage period in each storage place. Porang tubers should not be stored for more than two weeks and placed on a rack to minimize the damage of the tubers.Keywords: Physical change, porang tubers, storage time.

Co-Authors Afifi, Muhammad Naufal Al Adawiah, Putri Rabiah Aminatun Munawarti, Aminatun Anggraeni In Oktavia Apensa, Varni Arik Arubil Fatinah Dhike Ardhian Dwinianti, Edia Fitri Estri Laras Arumingtyas Fatinah, Arik Arubil Juliandari, Ria Reinnata Nahampun, Hartinio Natalia Novelia, Kiki Riska Nunung Harijati Nurul Chairiyah Rahajeng, Saptini Mukti Ria Reinnata Juliandari Rodiyati Azrianingsih Rosyidah, A’liyatur Savitri Nurlaila Wahyu Widoretno Wardani, Imaniah Bazlina Yasminatul Khoiroh Yasminatul Khoiroh

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