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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences The Journal of Experimental Life Sciences (JELS) Biotropika BIOWALLACEA Research Journal of Life Science Jurnal Penelitian Pendidikan IPA (JPPIPA) Medfarm: Jurnal Farmasi dan Kesehatan
Retno Mastuti
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Physics Public Health

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Microsatellite Marker for Genetic Variation Analysis in Local Chili Pepper (Capsicum frutescens L.) Induced by Ethyl Methane Sulfonate (EMS) Ria Reinnata Juliandari; Retno Mastuti; Estri Laras Arumningtyas
Journal of Tropical Life Science Vol. 9 No. 2 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.09.02.08

Abstract

Mutation using Ethyl Methane Sulfonate (EMS) is a simple and quick method to produce genetic variation in chili pepper. In this study, a total of 3 genotypes of local chili pepper (Capsicum frutescens L.), i.e. Genotype 2 (G2), Genotype 7 (G7), and Genotype 11 (G11) were induced by EMS with concentrations of 0% (K0), 0.01% (K1), 0.02% (K2), and 0.04% (K3). Genetic variation analysis in mutant was performed based on 3 microsatellite markers CA 19, CA 27, CA 62. Those molecular markers successfully detected the genetic variation in chili pepper mutant based on the number and size of microsatellite alleles variation. The 3 genotypes of chili pepper mutant produced a total of 15 alleles with the average Polymorphism Information Content (PIC) value of 0.82. Compared to the control plant, genetic variations in genome level were observed in local chili pepper. Furthermore, the treatment of EMS with concentration of 0.04% produced the most notable genetic variation in 3 genotypes of local chili pepper. 
Assessing Exogenous L-Glutamine and PEG Influence on Soybean Adventitious Roots Culture: Lateral Root Morphology and Amino Acid Profiles: L-Glutamine and PEG Influence on Adventitious Roots Culture Ardhian, Dhike; Mastuti, Retno; Nahampun, Hartinio Natalia
Journal of Tropical Life Science Vol. 15 No. 1 (2025)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.15.01.01

Abstract

Soybean (Glycine max L. Merr.) plays an important role in global agriculture and provides a primary source of plant nutrition, notably amino acids. However, tropical climates often result in lower amino acid levels than subtropical climates. This study explored the effects of L-glutamine and PEG on enhancing the amino acid content in in vitro soybean adventitious root. In this study, in vitro adventitious roots from the Detap1 soybean variety were treated in a liquid medium with various concentrations of L-glutamine (0, 1, 2, 4, 8, and 16 g.L-1) or PEG (0, 10, 20, 40, 80, and 160 g.L-1) for 8 days. The root biomass was quantified, and the amino acid profile was analyzed using HPLC. L-glutamine or PEG treatments significantly reduced root biomass by inhibiting lateral root initiation. Meanwhile, 20 amino acids were detected in the control and L-glutamine or PEG treatments. The application of a 16 g.L-1 L-glutamine significantly increased amino acid content. PEG treatments also had similar results in root growth inhibition and increased amino acid accumulation. Multivariate analysis showed significant separation between the control and treatment groups, with glutamine playing a crucial role in the group separation. This study demonstrates the potential of in vitro technology to improve amino acid production through adventitious root culture, providing insights into stress responses and metabolic regulations
The Relationship of Chemical Compounds and Crystal Development in Porang Tuber (Amorphophallus muelleri Blume) Chairiyah, Nurul; Harijati, Nunung; Mastuti, Retno
The Journal of Experimental Life Science Vol. 13 No. 1 (2023)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2023.013.01.01

Abstract

Chemical compounds are presumed to interact with each other and potentially affect the crystal development of porang tubers. This study focused on glucomannan and calcium oxalate (CaOx) as chemical compounds found in porang. Crystal development was analyzed by microscopic observation. The tuber tissue was used for microscopic slides and was harvested two weeks before the plants lost their leaves, when the plants lost their leaves, and two weeks after the plants lost their leaves. The CaOx and glucomannan content was measured using a modified extraction method. The increasing of CaOx content tends to increase the number of CaOx crystals. The analysis showed that the crystal density could affect the increasing of tuber weight possibility. There was a development process in raphide crystals that showed by different sizes at three harvest times, which the others were not. The results also indicated that glucomannan might influence the crystal density and the CaOx content. It can be concluded that glucomannan can potentially induce CaOx synthesis and crystal count increase. Keywords: Caox, Crystal, Development, Glucomannan, Interact, Porang.
OPTIMIZATION OF GREEN SYNTHESIS OF GOLD NANOPARTICLES USING CHILI FRUIT EXTRACTS (Capsicum Sp.) Oktavia, Anggraeni In; Mastuti, Retno; Rosyidah, A’liyatur; Arumingtyas, Estri Laras
MEDFARM: Jurnal Farmasi dan Kesehatan Vol 14 No 1 (2025): Medfarm: Jurnal Farmasi dan Kesehatan
Publisher : LPPM Akafarma Sunan Giri Ponorogo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.48191/medfarm.v14i1.502

Abstract

The use of plant extracts as reductants in green synthesis of gold metal nanoparticles (AuNPs) requires optimal conditions of temperature, extract concentration and pH. The objective of this research is to identify the optimal synthesis conditions for producing gold nanoparticles (AuNPs). The reducing agents employed are extracts from three varieties of chili peppers: Katokkon, Cakra Hijau, and Paprika Merah. Variations of plant extract concentrations of 10%, 15%, and 20%, incubation temperatures of 25°C, 37.5°C, and 50°C, and pH levels of 5, 7, and 9. The optimization formula design was determined using Box-Behnken, Design Expert version 13.0. The formation of AuNPs was indicated by a change in the color of the solution from yellow to pink to purple. Surface plasmon resonance was observed in the range of 200-800 nm using a microplate reader. Chili, Katokkon, Cakra Hijau and Red Paprika extracts were able to synthesize Au into AuNPs. The optimal formula for synthesizing AuNPs using Katokkon and Red Paprika extracts at a concentration of 10% while Ckra Hijau at a concentration of 15%. The incubation temperature used was 37.5°C for Katokkon extract and 25°C for Cakra Hijau and Red Pepper extract. The pH level used for the synthesis of AuNPs was pH 5 for all chili pepper extracts.
The Effect of Basal Medium on Callus Induction and Plant Regeneration in Anther Culture of Rice (Oryza sativa L.) Iriani, Yoanita Fadlilah; Prayantini, Dian Catur; Nahampun, Hartinio Natalia; Mastuti, Retno; Harijati, Nunung
Jurnal Penelitian Pendidikan IPA Vol 11 No 8 (2025): August
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i8.11884

Abstract

Anther culture is an effective tissue culture technique for rapidly producing doubled haploid (DH) plants, significantly shortening the breeding cycle by 2-3 years compared to conventional methods. This study aimed to evaluate the effects of callus induction basal medium (B5 Gamborg and N6 Chu) and subsequent plant regeneration in both Japonica and Indica rice genotypes. The experiment was conducted using a completely randomized design (CRD) with two treatment factors. The first factor was genotype (G), comprising Nipponbare (G1), RCKJ 05 (G2), RCKJ 10 (G3), RCKJ 15 (G4), RCKJ 25 (G5). The second factor was the basal medium (M), consisting of N6 Chu medium (M1) and B5 Gamborg medium (M2). Each treatment combination (genotype x medium) was replicated three times, with one Petri dish (containing 125 anthers) per replicate. Parameters observed included callus induction frequency (CIF), green plantlet regeneration (RGP), and albino plantlet regeneration (RAP). The results demonstrated that both genotype and the basal medium for callus induction significantly influenced callus induction frequency and green plantlet regeneration.
Growth Response of Purple Corn (Zea mays var. Ceratina kulesh) to Endophytic Bacterial Biofertilizer Treatment Novelia, Kiki Riska; Mastuti, Retno; Rahajeng, Saptini Mukti
Biotropika: Journal of Tropical Biology Vol. 10 No. 2 (2022)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2022.010.02.02

Abstract

Jagung ungu merupakan salah satu komoditas jagung di Indonesia dengan kandungan antosianin yang tinggi sehingga baik bagi kesehatan. Upaya peningkatan hasil komoditas ini dilakukan dengan menggunakan pupuk hayati bakteri endofit yang banyak berpengaruh positif terhadap pertumbuhan serta kandungan metabolit sekunder pada tanaman. Penggunaan pupuk endofit terbukti mampu meningkatkan pertumbuhan dan hasil panen jagung kuning. Oleh karena itu penelitian ini bertujuan untuk mengetahui respon pertumbuhan tanaman jagung ungu terhadap pupuk hayati bakteri endofit. Penelitian ini menggunakan rancangan acak lengkap dengan dua faktor perlakuan yaitu pupuk hayati bakteri endofit dengan dosis 0 ml/l (kontrol, P0), 80 ml/l (P1), dan 90 ml/l (P2) dan umur tanaman yaitu 21, 35, 49, dan 63 HST. Pengaruh kedua faktor diamati pada pertumbuhan vegetatif sedangkan pada pertumbuhan generatif dan pasca panenhanya diamati pengaruh dosis pupuk. Parameter pertumbuhan vegetatif yang diamati meliputi tinggi tanaman, jumlah daun, panjang dan lebar daun. Parameter pertumbuhan generatif dan pasca panen yang diamati adalah umur berbunga, jumlah tongkol per tanaman, pajang tongkol, bobot basah dan kering tongkol serta bobot 100 biji jagung ungu. Hasil penelitian menunjukkan bahwa dosis pemupukan dan umur tanaman berpengaruh nyata terhadap tinggi tanaman, serta jumlah, panjang dan lebar daun. Dosis pemupukan berpengaruh yang nyata terhadap waktu berbunga, panjang tongkol, bobot basah dan bobot kering tongkol jagung ungu, namun belum memberikan pengaruh yang nyata terhadap bobot 100 biji jagung.
Co-Authors Anggraeni In Oktavia Arik Arubil Fatinah Dhike Ardhian Dwinianti, Edia Fitri Estri Laras Arumingtyas Fatinah, Arik Arubil Iriani, Yoanita Fadlilah Juliandari, Ria Reinnata Nahampun, Hartinio Natalia Novelia, Kiki Riska Nunung Harijati Nurul Chairiyah Prayantini, Dian Catur Rahajeng, Saptini Mukti Ria Reinnata Juliandari Rosyidah, A’liyatur Savitri Nurlaila Yasminatul Khoiroh Yasminatul Khoiroh