Sudarno, Sudarno
Departemen Manajemen Kesehatan Ikan Dan Budidaya Perairan, Fakultas Perikanan Dan Kelautan Universitas Airlangga, Indonesia.

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Pengaruh Perbedaan Konsentrasi Gliserol dalam Susu Skim Kuning Telur untuk Proses Penyimpanan Sperma Beku terhadap Motilitas dan Viabilitas Spermatozoa Ikan Patin (Pangasius pangasius) [Effect Of Different Glycerol Concentration In Skim Milk And Egg Yolk Diluter For Storage Process Of Frozen Semen On Sperm Motility And Viability Of Catfish (Pangasius pangasius)] Sudarno Sudarno; Bagus Rizki Novianto; Endang Dewi Masithah
Jurnal Ilmiah Perikanan dan Kelautan Vol. 6 No. 1 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v6i1.11356

Abstract

Abstract Needs of catfish seed always increase every years, especially for farming activities. Fulfillment demand of seed in large quntities and continuing are the main obstacle in the production process of catfish. This can be overcome by the process of cryopreservation. This process requires diluents and cryoprotectants to maintain the fertility of spermatozoa. Skim milk and egg yolks diluents has been used on fish because it was applied to carp and showing a good result of sperm motility and fertilization rate. Glycerol as cryoprotectant was reported effective to be used for European Catfish than the other kind cryoprotectant. This study aimed to determine the effect of different concentrations of glycerol in the diluent skim milk and egg yolk on the motility and viability of catfish (Pangasius pangasius) spermatozoa after freezing. This research method is the experiment with completely randomized design (CRD) as the experimental design. The treatment used is different glycerol concentrations, P1 (11%), P2 (13%), P3 (15%), and P4 (17%) and each of the treatment was repeated 5 times. The main parameters measured were motility (%) and viability (%) of spermatozoa. Supporting parameters include odor, color, pH, viscosity, sperm concentration and catfish (Pangasius pangasius). Analysis of the data using Analysis Of Variance (ANOVA) and to determine the best treatment performed Duncan's Multiple Range Test with 95% confidance interval. The results showed that the effect of the concentration of glycerol in the diluent skim milk and egg yolk was not significantly (P> 0.05) on the motility and viability of catfish (Pangasius pangasius) spermatozoa after freezing. Further testing needs to be done on the level of fertility of frozen sperm, as well as the growth rate of seeds produced from sperm frozen catfish (Pangasius pangasius) products
Ibm Bagi Petani Benih Udang Windu Skala Rumah Tangga (Backyard) Di Desa Kalitengah Kecamatan Tanggulangin Sidoarjo Yang Mengalami Gagal Panen Berkepanjangan Karena Serangan Penyakit [Ibm For Seed Shrimp Farmers Family Scale (Backyard) In Kalitengah Village, Tanggulangin District, Sidoarjo Region, That Harvesting Lossed To Long Times That Caused By The Diseases] Gunanti Mahasri; Sudarno Sudarno; Endang Dewi Masithah
Jurnal Ilmiah Perikanan dan Kelautan Vol. 6 No. 1 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v6i1.11378

Abstract

Abstract Demand of seed shrimp, as specially tiger shrimps is still not enough until now, it is only 5060%, more than for 5-10 years end showed decreased point. One of factors that influenced the successfully seeds shrimp hatchery is water quality that as a life media of shrimps. A bisnis  about shrimp hatchery is still have a good market, because there are a lot of tiger shrimp pond operational, more than some time demand of the  shrimp increase fluctuative on seasonal.  The aims of this this societies service activities is applicated a new shrimp hatcher technology by using immunostimulant at Putri Mandiri Group company, it aplicated in family hatcher in  Kalitengah village, Tanggulangin  District,  Region of Sidoarjo. The immunostimulant use to increase the body deffence of the shrimp larve in hatchery to the disease attacked dan invirontment during culture periode, it will be increase the harvesting.  The method using in the activity were socialitation/counseling, dempond and guiding to application of the method of shrimp hatcher by using immunostimulant in one periode. Monitoring and evaluation about this result were done in one month after the activity ending. This result showed that aplicated immunostimulant in shrimp family  hatcher Backyard) can increased the shrimp seed harvesting of Putri Mandiri Company owner, from 900.000 to 1.600.000 shrimp seeds, it is same as that the profit increased from  8.622.000,- until  15.822.000,-  Rupiahs for one periode panen for one   container 10 tonage capacity.
Uji Potensi Antifungi Perasan Daun Seledri (Apium graveolens L) Terhadap Aspergilllus terreus Secara In Vitro [Antifungal Potential Test Of Celery Leaves Juice (Apium graveolens L) Against Aspergilllus terreus By In Vitro] Sudarno Sudarno; Illa Rohdiana Hermawati; Didik Handijatno
Jurnal Ilmiah Perikanan dan Kelautan Vol. 6 No. 1 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v6i1.11379

Abstract

Abstract Aspergillus terreus is a fungal that is able to produce several mycotoxins such as patulin, sitrinin and aflatoxin and contained in processed mackerel mixed with fresh steamed way. The impact of the use of chemicals to control fungal attack A. terreus showed a negative effect. This study aims to determine the potential and the minimum concentration of the juice of the leaves of celery (Apium graveolens L) as antifungal against the growth of A. terreus in vitro. The experiment was conducted at the Laboratory of the Faculty of Fisheries and Marine Airlangga University in July 2013. The method was used in this research that using paper disc diffusion method. Treatment outcome data were analyzed using descriptive statistics. Test potential antifungal celery juice in inhibiting the growth of fungus A. terreus obtained negative be results. The concentrations of juices were given as a treatment of 10 % to 100 % are not able to inhibit the growth of A. terreus shown with no inhibition zone formation in all treatments except the concentration of 10 % formalin as a control (+).
Ibm bagi Petambak Udang Tradisional di Desa Masaran, Kecamatan Banyuates, Kabupaten Sampang, yang Gulung Tikar Akibat Kasus Kematian Udang yang Terus Menerus [ Ibm For Traditional Shrimp Farmers In Masaran Village, Banyuates District, Sampang Region, That Capital Losted By Shrimp Dead Cases Continuoesly] Sudarno Sudarno; Gunanti Mahasri; Kismiyati Kismiyati
Jurnal Ilmiah Perikanan dan Kelautan Vol. 6 No. 1 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v6i1.11383

Abstract

Abstract Tiger shrimp (Penaeus monodon Fab) is one of the economically important shrimp, until 1992 became the most important of non petroleum export commodity from fishery sector. Since the end of 1993 up to now, the Penaeus monodon Fab death level has been relatively high and due to this circumstance have been caused many ponds collapsed so that the shrimp production was dramatically declined for year by year. Banyuates District is one of the Sampang Region areas which have big fisheries potential, aspecially for the breakist water pond, that the topest as the other district. There are a lot of shrimp dead cases until now. But, so that 80% of breakist water pond were broken and not operational. The objective of this societies service activities is applicated a new shrimp culture technology with traditional plus Imuno-Biocirculation System. Imuno-Biocirculation System (SI-PBR) for increases the shrimp harvest at Candi District Region of Sidoarjo, at May until Desember 2013. The method using in the activity were socialitation/counseling, dempond and guiding to application of the SI-PBR model in one periode. Monitoring and evaluation about this result were done in one month after the activity ending. This result showed that a positive indication. There was the knowledges of the farmer in ceases by socialization, it also applicated a model in the right method for shrimp culture. There were also showed that the SI-PBR model can in ceased the shrimp harvest from 272,43 kg/ha to 854,66 kg/ha, it means was increased 313%. The conclution of this activity is the SI-PBR model can increased the shrimp harvest and can applicates in more larges area in Sidoarjo Region.
Pengaruh Biofilter Rumput Laut Gracilaria sp. terhadap Dominansi Plankton pada Media Air yang Terpapar Logam Berat Cr [Effect Of Biofilter Of Seaweed Gracilaria sp. To Plankton Domination On Water Medium That Exposed To Heavy Metal Chromium (Cr) ] Sudarno Sudarno; Tantika Wulan Sari; Amin Alamsjah
Jurnal Ilmiah Perikanan dan Kelautan Vol. 5 No. 1 (2013): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v5i1.11416

Abstract

Abstract Pollution is entered or the inclusion of living things, matter, energy, and or other components into the water. The study shows that the qualitative waters affected Sidoarjo mud Lapindo has the value of pollution index (13.3433), Sidoarjo heavily polluted coastal waters, and to sustain the cultivation of water treatments required physical, chemical and biological before the water is used for maintenance (Sudinno, 2009). Associated with the food chain, plankton have properties capable of accumulating pollutants in the environment. Pollutants in the environment are accumulated by plankton forming toxin becomes toxic compounds. There is a selection of flora and fauna to be used either as a biofilter, bioakumulator well as pollution biomonitoring agent occurs in the waters of the sea grass Gracillia sp. Giving Gracilaria sp. with different doses expected to affect the dominance of plankton in the water media exposure of heavy metals Cr. Once the research is done, it turns out the levels of heavy metals chromium (Cr) has been dropped from the initial 0.18 mg / l to 0.09 mg / l in the sediment. There Tetraselmis chuii plankton dominance in aqueous media, dominance of plankton in the water medium to portray the diversity of aquatic environments with low plankton, and this is not good for plankton and other organisms that utilize the natural feed.
Distribusi Penyakit Infectious Myo Necrosis Virus (IMNV) Pada Udang Vannamei (Litopenaeus vannamei) Di Pantai Utara Jawa Timur [ Distribution Of Infectious Myo Necrosis Virus (IMNV) In White Shrimp Vannamei (Litopenaeus vannamei) In North Coast, East Java] Laksmi Sulmartiwi; Andre Rekasana; Sudarno Sudarno
Jurnal Ilmiah Perikanan dan Kelautan Vol. 5 No. 1 (2013): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v5i1.11424

Abstract

Abstract White shrimp vannamei (Litopenaeus vannamei) is the main commodities among shrimp farmers because it has a rapid growth, can be cultivated with high density and world market demand is large enough .Since being introduced in Indonesia, vannamei shrimp has spread to the north of East Java pesisisr like Probolinggo, Pasuruan, Lamongan and Tuban. Shrimp growth factors are must be faced on vannamei shrimp culture, including the problems of disease. One type of virus disease is an Infectious Myo Necrosis Virus (IMNV). IMNV has affecting to the 70% of shrimp mortality (Tang et al., 2008). This study aims to determine the distribution of IMNV on white shrimp vannamei from the northern coast of East Java. This research uses descriptive method by taking samples from five different location which 75 samples totaly. In this research also take the water quality parameter including pH, DO, salinity and temperature. Whole of the samples performed the PCR test to determine how many samples which is indicating of IMNV. Based on measurements of water quality at five different locations, the plots I, II and III obtained the temperature range between 29.3-30.2 °C, salinity 90-30 ppt, pH 7.5-7.9, and DO 3.8-5.7 ppm. Temperature, pH and DO parameters show normally on white shrimp vannamei, while salinity shows different valve from normal conditions. Abnormallity happened in Situbondo with the salinity betwen 32-33 ppt and Pasuruan betwen 9-10 ppt. Results showed that the number of the lowest infection is Lamongan (53%) following Probolinggo (60%), Tuban (67%), Pasuruan (73%) and the highest is from Situbondo (100%)
Induksi Kematangan Gonad Induk Jantan Kerang Abalone (Haliotis asinina) dengan Metode Laserpunktur [Induction Of Gonad Maturity Males Broodstock Abalone Shell (Haliotis asinina) With Laserpunctur Methods ] Sudarno Sudarno; Rahayu Kusdarwati; Aprilia Putri Astutie
Jurnal Ilmiah Perikanan dan Kelautan Vol. 4 No. 1 (2012): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v4i1.11576

Abstract

Abstract Abalone is one kind of shellfish fisheries of the world has become a commodity which is currently experiencing increased demand, especially from international markets. As one initial step of a series of required maintenance management process of cultivating a good broodstock, to get the broodstock with the maximum level of gonad maturity is by induction using laserpunctur. Laserpunctur appropriate technology has been shown to accelerate the growth process, increase and accelerate gonadal maturation and spawning shorten the reproductive cycle of some species. The purpose of this study was to determine the effect laserpunctur fired on gonadal organs of the male gonadal maturity of abalone shells and the best energy of the male gonadal maturation affect abalone shells. The treatment used is laserpunctur irradiation on the male gonad organ abalone shells with a energy of 0 Joule (control), 0.5 Joules for 50 seconds, 1 Joule for 100 seconds, 1.5 Joules for 150 seconds and 2 Joule for 200 seconds. Each treatment was repeated four times. The results of this study was the laserpunctur irradiation fired on the gonadal organs influence the development of gonadal maturity of male broodstock abalone shells. The best energy of laserpunctur in the male gonad maturation affects the abalone shells is 1.5 Joules for 150 seconds. The results of histological observation of gonadal male broodstock H. asinina showed varying degrees of gonadal development in the process of gametogenesis is marked with an individual's reproductive cycle.
Uji Sensitifitas Sari Buah Pare (Momordica charantia L) pada Bakteri Edwardsiella tarda dengan Metode Difusi Kertas Cakram Secara In Vitro [Sensitivity Test Of Fruit Bitter Melon Juice (Momordica charantia L) On Bacteria Edwardsiella tarda With Paper Disc Diffusion Method In Vitro] Sudarno Sudarno; Selvi Lely Rosanti; Sri Subekti
Jurnal Ilmiah Perikanan dan Kelautan Vol. 4 No. 1 (2012): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v4i1.11593

Abstract

Abstract One of the problems encountered in fish farming is the disease attack. The attack of disease that was caused by bacteria is a problem that often occur in aquaculture. Traditional medicine is more readily accepted by communities because is cheap and easily available, have been quite familiar with the community. There were various kind of traditional medicine and has been widely studied chemically content and usefulness. Therefore there were still many plants unknown levels of toxicity, therefore need further study. Bacteria E. tarda spread in some countries such as Europe, Japan, Taiwan, Thailand, United States, Singapore, and Malaysia. While in Indonesia E. tarda were found in Java, Sumatra, and Borneo. The aim of the research is to know the capabilities of the inhibition zone of fruit bitter melon power against the bacteria E. tarda in vitro and knowing whether fruit bitter melon effective in inhibiting the growth of the bacteria E. tarda with gold standard comparator antibiotics Tetracycline. The research was conducted on February 2011 in Bacteriology and Microbiology, laboratory in Fish Quarantine Juanda Sidoarjo district of East Java. The method used in this research is descriptive method that was done in vitro by using sensitivity test of diffusion method with the determination of inhibition zone by comparing with antibiotic inhibition zones. The results showed that bitter melon juice has a bacterial growth inhibition against E. tarda, although when was compared with gold standard antibiotic tetracycline shared considered less sensitive. Observations showed clear zone of bitter melon fruit juice shared with a diameter of 13.3mm, was compared with standard antibiotic tetracycline can inhibit with a minimum diameter of 15mm. Sensitivity test of bitter melon fruit juice will be classified as not susceptible to the bacteria therefore is able to inhibit E. tarda..
Pemberdayaan Pembudidaya Ikan dan Udang Tambak, Desa Kendalkemlagi, Kecamatan Karanggeneng, Kabupaten Lamongan, Propinsi Jawa Timur [Empowerement For Fish and Prawn Farmers In Pond At Kendalkemlagi Village, Karanggeneng District, Lamongan Regency, East Java Province ] Muhammad Arief; Akhmad Taufiq Mukti; Sudarno Sudarno
Jurnal Ilmiah Perikanan dan Kelautan Vol. 3 No. 2 (2011): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v3i2.11596

Abstract

Abstract The aim of this IbM program was empowerement for fish and prawn farmers through good conducting and correctness management of fish and prawn cultures in pond. Activity method were discussion and training on management of fish and prawn culture in pond.. Result of this IbM program were improve knowledge and skill of fish and prawn farmers to manage well and correctness of fish and prawn cultures in pond. This program can used to effort increase production yield of fish and prawn cultures in pond.
IbM Kelompok Petambak Tradisional Udang Windu Di Desa Kedung Peluk, Yang Menghadapi Penurunan Hasil Panen Secara Drastis Sejak Tahun 1994 [IbM For Windu Shrimp Small Scale Farmer Traditional In Kedung Peluk Village Whose Gets Harvesting Decreased Since 1994 ] Gunanti Mahasri; A. Shofy Mubarak; Sudarno Sudarno
Jurnal Ilmiah Perikanan dan Kelautan Vol. 3 No. 2 (2011): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v3i2.11603

Abstract

Abstract Tiger shrimp (Penaeus monodon Fab) is one of the economically important shrimp, until 1992 became the most important of non petroleum export commodity from fishery sector. Since the end of 1993 up to now, the Penaeus monodon Fab death level has been relatively high and due to this circumstance have been caused many ponds collapsed so that the shrimp production was dramatically declined for year by year. Candi District is one of the Sidoarjo Region areas which have big fisheries potential, aspecially for the breakist water pond, that the topest as the other district. There are a lot of shrimp dead cases until now. But, so that 80% of breakist water pond were broken and not operational. The objective of this societies service activities is applicated a new shrimp culture technology with traditional plus Imuno-Biocirculation System. Imuno-Biocirculation System (SIBR) for increases the shrimp harvest at Candi District Region of Sidoarjo, at June until November 2011. The method using in the activity were socialitation/counseling, dempond and guiding to application of the SIBR model in one periode. Monitoring and evaluation about this result were done in one month after the activity ending. This result showed that a positive indication. There was the knowledges of the farmer in ceases by socialization, it also applicated a model in the right method for shrimp culture. There were also showed that the SIBR model can in ceased the shrimp harvest from 272,43 kg/ha to 854,66 kg/ha, it means was increased 313%. The conclution of this activity is the SIBR model can increased the shrimp harvest and can applicates in more larges area in Sidoarjo Region.