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All Journal Dentika Dental Journal Dentino: Jurnal Kedokteran Gigi Dental Journal (Majalah Kedokteran Gigi) Indonesian Journal of Dental Medicine Indian Journal of Forensic Medicine & Toxicology Jurnal Abdi Masyarakat Indonesia Journal of Fisheries & Marine Karya Kesehatan Journal of Community Engagement

Seno Pradopo

Department Of Paediatric Dentistry, Faculty Of Dental Medicine, Universitas Airlangga

Author-ID : 3196083

Humanities Biochemistry, Genetics & Molecular Biology Dentistry Economics, Econometrics & Finance Education Engineering Health Professions Medicine & Pharmacology Nursing Public Health Veterinary

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Daya antibakteri penambahan Propolis pada zinc oxide eugenol dan zinc oxide terhadap kuman campur gigi molar sulung non vital (The antibacterial effect of propolis additional to zinc oxide eugenol and zinc oxide on polybacteria of necrotic primary molar) Yemy Ameliana; Herawati Herawati; Seno Pradopo
Dental Journal (Majalah Kedokteran Gigi) Vol. 47 No. 4 (2014): December 2014
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Materials commonly used for root canal filling of primary teeth is zinc oxide eugenol. Eugenol has some disadvantages that can irritate the periapical tissues, has the risk of disturbing the growth and development of permanent tooth buds, and has a narrow antibacterial spectrum. Studies showed that propolis at concentration of 20 % has antibacterial activity against Staphylococcus aureus. Purpose: The purpose of this study was to examine the antimicrobial activity of root canal pastes with the additional of propolis additional to zinc oxide eugenol (ZOEP) and to zinc oxide (ZOP). Methods: Polybacteria cultures collected from root canals of necrotic primary molar from 5 children patients who received root canal treatment. The bacteria were grown in BHI Broth, and inoculated into Muller Hinton Agar media. The agar plates was divided into 3 areas, and one well was made at each area. The first well filled with ZOE as a control, second well filled with ZOEP and the third well filled with ZOP, then incubated for 24 hour at 370 C. Antimicrobial activity was determined by measuring the diameters of inhibition zones of polybacteria growth. The data were statistically analyzed by independent T-test. Results: The pasta mixture of zinc oxide propolis had the strongest antibacterial activity against polybacteria of necrotic primary molar, followed by zinc oxide eugenol propolis paste, and zinc oxide eugenol paste. There were significant differences of inhibition zones between ZOE, ZOEP and ZOP (p<0,05). Conclusion: The study suggested that the additional of propolis to zinc oxide paste could increase the antimicrobial effect against root canal polybacteria of necrotic primary molar.Latar belakang: Bahan yang sering digunakan untuk pengisian saluran akar gigi sulung adalah zinc oxide eugenol. Eugenol memiliki beberapa kekurangan yaitu dapat mengiritasi jaringan periapikal, beresiko mengganggu pertumbuhan dan perkembangan benih gigi permanen pengganti, serta memiliki spektrum antibakteri yang sempit. Penelitian menunjukkan propolis dengan konsentrasi 20% memiliki daya antibakteri terhadap bakteri staphylococcus aureus Tujuan: Penelitian ini bertujuan meneliti efek aktivitas antimikroba pasta saluran akar dengan penambahan propolis pada zinc oxide eugenol (ZOEP) dan pada zinc oxide (ZOP) Metode: Kultur bakteri campur diperoleh dari saluran akar gigi molar sulung 5 pasien anak yang sedang dirawat saluran akar. Bakteri ditumbuhkan dalam BHI Broth dan diinokulasi ke Muller Hinton Agar media. Plate agar dibagi menjadi 3 bagian, dan setiap bagian dibuat satu sumuran. Sumuran pertama diisi dengan ZOE sebagai kontrol, sumuran kedua diisi dengan ZOEP dan sumuran ketiga diisi dengan ZOP, kemudian diinkubasi selama 24 jam, pada 37°C. Daya antimikroba ditentukan dengan mengukur diameter zona hambatan pertumbuhan bakteri campur. Data dianalisis statistik dengan t-test independent. Hasil: Pasta campuran zinc oxide propolis memiliki daya antibakteri terhadap kuman campur gigi molar sulung non vital paling kuat, diikuti pasta zinc oxide eugenol propolis, dan pasta zinc oxide eugenol. Terdapat perbedaan zona hambat yang signifikan diantara ZOE, ZOEP dan ZOP (p<0.05). Simpulan: Penelitian ini menunjukkan bahwa tambahan propolis pada pasta zinc oxide dapat meningkatkan efek antimikroba terhadap polybacteria saluran akar dari molar sulung yang nekrotik.

Daya antibakteri obat kumur chlorhexidine, povidone iodine, fluoride suplementasi zinc terhadap, Streptococcus mutans dan Porphyromonas gingivalis (Antibacterial effect of mouth washes containing chlorhexidine, povidone iodine, fluoride plus zinc on Strep Betadion Rizki Sinaredi; Seno Pradopo; Teguh Budi Wibowo
Dental Journal (Majalah Kedokteran Gigi) Vol. 47 No. 4 (2014): December 2014
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Dental Caries and periodontal disease prevalence in Indonesian children are still high. Some efforts can be done to overcome the problem; one of them is the use of mouthwash to decrease pathogen microorganisms. The mouthwashes that commercially available in market are chlorhexidine, povidone Iodine and Fluoride with Zinc supplementation. Purpose: The purpose of this study was to examine the anti bacterial effect of the mouthwashes chlorhexidine, povidone iodine and fluoride with zinc supplementation against mix bacteria that found in the plaque, Streptococcus mutans and Porphyromonas gingivalis. Methods: The antibacterial effect was measured using disk diffusion test. The bacteria samples (plaque polybacteria, S.mutans and P. gingivalis) were inoculated and spread in the petridish containing MHA. Paper discs containing the mouthwashes were placed in the petridish and incubated for 24 hours at 37oC (anaerobe for P. gingivalis, aerobe for S. mutans and polybacteria). The diameter of inhibition zone surrounding the paper discs were measured and compared between each active ingredient contained in mouthwash. Results: Chlorhexidine had the strongest antibacterial effect than povidone iodine and fluoride. Chlorhexidine was more effective to inhibited the growth of S. mutans than to polybacteria or P.Gingivalis, while Povidone iodine and fluoride were more effective to inhibited the growth of polybacteria. Conclusion: The mouthwash chlorhexidine was more effective to inhibit the growth of plaque polybacteria, Streptoccous mutans and Porphyromonas gingivalis compared with povidone iodine and fluoride with zinc supplementation.Latar belakang: Prevalensi karies gigi dan penyakit periodontal masih tinggi pada anak Indonesia. Usaha mengatasi hal tersebut antara lain melalui melalui penggunaan obat kumur untuk mengurangi jumlah kuman pathogen. Kandungan obat kumur yang beredar di pasar diantaranya adalah chlorhexidine, povidone iodine dan fluoride dengan suplementasi zinc. Tujuan: Penelitian ini bertujuan untuk meneliti efek antibakteri dari obat kumur berbahan aktif chlorhexidine, povidone iodine dan fluoride dengan suplementasi zinc terhadap bakteri campur plak, S. mutans dan P. gingivalis. Metode: Pengukuran efek antibakteri dilakukan dengan metode disk diffusion. Bakteri sampel (bakteri campur plak, Streptococcus mutans dan Porphyromonas gingivalis) ditanam secara merata pada cawan petri dengan medium MHA. Cakram kertas yang mengandung obat kumur diletakkan di tengah cawan petri dan diinkubasi selama 24 jam pada suhu 37o C (anaerob untuk P. gingivalis, aerob untuk S.mutans dan bakteri campur). Diameter zona hambat bakteri yang mengelilingi cakram kertas diukur dan dibandingkan antara masing-masing bahan aktif yang terkandung dalam obat kumur. Hasil: Chlorhexidine mempunyai efek antibakteri paling kuat dibanding povidone iodine dan fluoride. Chlorhexidine lebih ampuh menghambat pertumbuhan bakteri S.mutans dibanding terhadap bakteri P.gingivalis dan bakteri campur dalam plak, sedang Povidone iodine dan fluoride lebih efektif menghambat pertumbuhan bakteri campur. Simpulan: Obat kumur chlorhexidine lebih efektif dalam menghambat pertumbuhan bakteri campur dari plak, Streptococcus mutans dan Porphyromonas gingivalis dibanding povidone iodine dan fluoride dengan suplementasi zinc.

Allergic asthma in children: Inherited, transmitted or both? (The transmission of periodontopathic bacteria concept) Seno Pradopo; Haryono Utomo
Dental Journal (Majalah Kedokteran Gigi) Vol. 42 No. 3 (2009): September 2009
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: In theory, allergic diseases including asthma, was the resultof exposure to a transmissible agent and do not depend on early infection which is said to make children more allergy-resistant. This seems, to be a direct contradiction to the hygiene hypothesis, since epidemiologic evidence can be cited in this theory's support. The fact that nearly all childrenwith asthma are allergic, but only a small proportion of allergicchildren have asthma, at least raises the possibility that someadditional factor is involved. That this additional factor might be a transmissible agent is also suggested by the similarity between the gross epidemiologic patterns of children with paralyticpoliomyelitis in the 1950s and children with asthma currently. Purpose: The purpose of this study was to reveal the possible relationship between the transmissions of allergic asthma and periodontopathic bacteria. Reviews: Recent researches showed that periodontopathic bacteria are transmissible from mother and caregivers to infants. In addition, a collaborated research that was conducted by dental practitioners and pediatricians revealed that Gram-negative bacteria were significantly predominant (p = 0.001) in uncontrolled allergic asthmatic children compared to well-controlled ones. Nevertheless, how does these two phenomenon related was still uncertain. Literatures showed that periodontopathic bacteria modulates host immune response and sometimes caused disadvantageous effect to allergic asthma. Conclusion: According to the ability of periodontopathic bacteria and its components to stimulate immunocompetent cells, it is possible that they are able to modify host-immune response which tends to increase allergic asthma symptoms.

The colony number of Streptococcus mutans and Lactobacillus in saliva of dental caries and free caries children Seno Pradopo
Dental Journal (Majalah Kedokteran Gigi) Vol. 41 No. 2 (2008): June 2008
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Streptococcus mutans (S. mutans) are regarded as the main initiator microorganism of caries, with Lactobacilli participating on caries progression, due to its carcinogenic capacity. The purpose of this study was to examine the number of S. mutans and Lactobacillus sp in children with in children’s saliva with dental caries and free caries. Twenty children attending the Paediatric Dental Clinic in Airlangga University participated in our study. Their age ranged from 1–14 years old. Subject was divided into two groups, which were study group consisting of 10 children with 3–5 dmft/DMFT and control group with 10 caries free children. Subjects were examined and their caries number was recorded using WHO index. Stimulated saliva was collected from each subject for bacterial assessment. Colony counting of S. mutans and Lactobacillus sp count in each saliva sample group were done. The study showed that subject with 3–5 dmft/DMFT had higher number of S. mutans and Lactobacillus sp than caries free.

The role of partial denture in management of hypohidrotic ectodermal dysplasia Tania Saskianti; Seno Pradopo; Prawati Nuraini; Michael Josef Kridanto Kamadjaja
Dental Journal (Majalah Kedokteran Gigi) Vol. 41 No. 2 (2008): June 2008
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Ectodermal dysplasia is a rare hereditary disorder with a characteristic physiognomy. The ectodermal dysplasia constitutes a group of hereditary disorders whose clinical manifestation can be defects in ectodermal structures. The case of a 11-year-old child with hypohidrotic ectodermal dysplasia and partial anodontia is presented. Affected children require extensive dental treatment to restore appearance and help the development of a positive self image. Partial denture was provided to encourage a normal psychological development and to improve the function of the stomatognatic system. It is important for the patient and the dentist to understand that continued monitoring for dental problems is necessary. This paper had an objective to relate and discuss a case of hypohidrotic ectodermal dysplasia, with the approach of the influence of an esthetic rehabilitation and functional alternative in the improvement of the quality of life.

Gingival immunologic defense index: a new indicator for evaluating dental plaque infection risk in allergic children Seno Pradopo; Haryono Utomo
Dental Journal (Majalah Kedokteran Gigi) Vol. 41 No. 1 (2008): March 2008
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

There is a possible relationship between dental plaque and children allergic diseases. According to literatures, gingivitis suffered mostly by allergic children than control. Case reports also revealed that dental plaque control therapy was able to reduce, even eliminate rhinosinusitis and asthmatic symptoms without additional medications. However, the exact method for confirming the gingivitis-related allergy is still uncertain. Allergic diseases have multifactorial etiologies and dental plaque had been proposed as a new trigger of allergic symptoms. Nevertheless, since not every child with gingivitis suffered from allergy or vice versa, this uncertain phenomenon may lead to patients or other clinician disbelief. The objective of the present study was to propose a new method, which involving the Gingival immunologic defense index (GIDI) to evaluate the susceptibility to allergic diseases. GIDI is an index that had been developed earlier for evaluating gingival immunologic defense with respect to immunoglobulin A (IgA) levels. This index based on the simple count of the inflamed gingival surfaces of a child plus the measurement of salivary IgA content. It provides clinicians with important information about the immunologic defense potential of each subject. Interestingly, most allergic children also had inherited IgA deficiency, thus this concept is likely. Based on literatures, GIDI could be a potential index for evaluating the risk of allergic diseases through gingival health assessment. However, prior investigation to the value of Indonesian GIDI index which related to allergy should be conducted.

New insight in pediatric dentistry: preventive dentistry in allergy management protocol Seno Pradopo; Haryono Utomo
Dental Journal (Majalah Kedokteran Gigi) Vol. 40 No. 3 (2007): September 2007
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

; "> The relationship between oral health and systemic diseases had been abundantly studied, however, mostly were related to adultsuch as cerebrovascular disease, cardiovascular disease, diabetes mellitus etc. Nevertheless, it was still uncommon that oral healthalso related to allergic disease. The field of pediatric dentistry is mostly related to preventive dentistry (i.e. prophylactic procedures,preventive orthodontic etc., but rarely related to preventive medicine such allergy prevention in children. Allergic diseases develop outof a close interaction between genetic predisposition and environmental triggers, and progress continuously since infancy regarding tothe allergic march. Concerning to the partially developed immunity in children, children are more susceptible to infection and allergicdiseases than adults. Unfortunately, infection and allergic diseases are interrelated; infection impaired allergy and vice versa. Poororal health is closely related to infection; however, improving oral health is not included in allergy management protocol. In order toanticipate the future, dentist or especially pediatric dentist should be able to review about basic children immunity and oral mucosalimmunity. Additionally, it is essential to explain to the parents and medical practitioners who are not familiar to this new paradigm.The objective of this study is to review articles related to children’s oral health and allergic symptoms. Regarding to the successfuloral management of allergic symptoms, the propensity that improving oral health could be included in children’s allergy managementprotocol is likely.

Streptococcus mutans detection on mother-child pairs using matrix-assisted laser desorption ionization – time of flight mass spectrometry and polymerase chain reaction Udijanto Tedjosasongko; Dwi Mulia Ramadhaniati; Seno Pradopo
Dental Journal (Majalah Kedokteran Gigi) Vol. 54 No. 1 (2021): March 2021
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v54.i1.p52-56

Background: Streptococcus mutans (S. mutans) bacteria mainly cause dental caries in children. These bacteria are not considered oral indigenous bacteria since they are transmitted from people around children during their deciduous teeth eruption. The detection of these bacteria can be used for dental caries prevention in children. Purpose: To determine the strain and serotype of S. mutans by using matrix assisted laser desorption ionization – time of flight mass spectrometry (MALDI-TOF MS) and polymerase chain reaction (PCR) on dental plaque samples taken from mother-child pairs. Methods: Sixteen dental plaque samples of mother-child pairs were cultured on brain heart infusion broth (BHIB) and mitis salivarius bacitracin (MSB) media until S. mutans colony isolates were obtained. Next, the isolates of S. mutans colony were introduced into the target plates of MALDI-TOF MS, and then ionized to become peptide mass fingerprint (PMF). Afterwards, the colony isolates were detected by database software. The detected S. mutans DNA then was extracted by using conventional 727 bp PCR (serotype C). Results: Six strains of S. mutans were detected by MALDI-TOF MS method. Five samples were classified into UA159, two samples were 3SN1, two samples were NFSM1, two samples were 11A1, two samples were U138, two samples were 4SM1, and one sample was classified into another bacterium. Five out of 16 samples were detected by PCR as serotype C (UA159). Conclusion: Six strains of S. mutans were detected, namely UA159, 3SN1, NFSM1, 11A1, U138, and 4SM1, one of them (UA159) was detected as serotype C.

A comparison of the accuracy of the cervical vertebrae maturation stage method and Demirjian’s method on mandibular length growth Alfira Putriana Dewi; Seno Pradopo; Sindy Cornelia Nelwan
Dental Journal (Majalah Kedokteran Gigi) Vol. 55 No. 1 (2022): March 2022
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v55.i1.p13-15

Background: Malocclusion is one of the most familiar dental problems, with a high prevalence among the population. Understanding the patient’s craniofacial growth and development is crucial in diagnosis, as are the planning and subsequent success of the treatment. Malocclusion needs to be treated early to optimise the outcome achieved by the treatment. One of the most common types of malocclusions observed in clinics is crowding. The craniofacial bone relevant to the treatment of crowding is the mandible, defined as the mandibular length from the condylion to gnathion areas. When planning treatment, clinicians may experience difficulties in determining the biological age of patient, particularly when supporting diagnostic tools are not available. The indicators of biological age can be observed by the assessment of bone maturation using the cervical vertebrae maturation (CVM) method and by the analysis of tooth maturation using Demirjian’s method. However, limited studies are available regarding the accuracy of these methods as diagnostic tools. Purpose: This study aims to analyse the accuracy of the CVM method compared with Demirjian’s method concerning mandibular length growth. Methods: An analytic research method and a cross-sectional design are employed. The research sample comprised 50 lateral cephalometric and panoramic photos of children aged 8-16 years. Data were collected by analysing the maturity level of the cervical vertebrae and the teeth, and measuring the mandible length of the children in the photos. The statistical test used was the Wilcoxon test. Results: The results of the Wilcoxon test for the asymptotic sign had a p-value of 0.116 > 0.05, indicating no significant difference between the CVM and Demirjian methods. Conclusion: Both of the methods noted above yielded equally accurate results for determining mandibular length growth.

Cervical vertebral maturation stage and Demirjian index for assessment of skeletal and dental maturation for children’s growth stages Pradopo, Seno; Nuraini, Prawati; Rahmawati, Luluk; Ibrahim, Zurairah
Dental Journal (Majalah Kedokteran Gigi) Vol. 58 No. 1 (2025): March
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v58.i1.p60-65

Background: Determining age is essential in various fields, particularly pediatric dentistry. A reliable method for assessing an individual's growth and development involves evaluating their chronological and biological age. Biological age can be determined by examining skeletal or dental maturation. Accurately assessing growth potential and timing of growth spurts is crucial for several clinical situations, especially in the planning and outcomes of treatments like orthodontic therapy. Purpose: This study uses common radiographic ortho-diagnosis techniques to analyze the accuracy of cervical vertebral maturation stages (CVMS) and the Demirjian index methods in evaluating children's growth and development stages. Methods: The CVMS assessment on cephalometric radiography was conducted using the Bacetti method, which includes six stages. In addition, the mandibular second molars' calcification stages were evaluated using the Demirjian index method, which encompasses stages A to H on panoramic radiography. Following this, skeletal and dental maturation accuracy was analyzed using the Statistical Package for Social Sciences application. Results: This study revealed differences in the chronological age corresponding to each stage of calcification of the mandibular second molars and the CVMS. Additionally, CVMS was found to be the most accurate method for assessing age in children. Furthermore, the right side was generally preferred over the left at the calcification stages of the mandibular second molars. Conclusion: Using CVMS to assess skeletal maturation provides a more accurate determination of growth and developmental stages in children than the Demirjian index.

Co-Authors Achmad Nadian Permana Achmad Nadian Permana Ainuddin Yushar Md Yusof Alfira Putriana Dewi Amalia Wimarizky Amalia Wimarizky Ardianti Maartrina Dewi Arlina Nurhapsari Audrey, Natasha Winona Az Zahra, Alivy Aulia Brian Maulani Destyka Fridiana Diana Md Zahid Dwi Mulia Ramadhaniati Felita Putri Sucipto Hartman Tanzil Haryono Utomo Herawati Herawati Ibrahim, Zurairah Ichyana, Devalna Siwi Ilvana Ardiwirastuti Indasari, Novia Islamy Rahma Hutami, Islamy Rahma Khairani, Firli Cahaya Masyithah Masyithah, Masyithah Maulidina Nabilah Tasyakuranti Mayangsari, Regilia Shinta Meidy Damayanti Michael Josef Kridanto Kamadjaja Nadira Jasmin Nelwan, Sindy Cornelia Prawati Nuraini Puspita Ayuningtyas R. Darmawan Setijanto Rahmawati, Luluk Regina Ayu Pramudita Renie Kumala Dewi Ristya Widi Endah Yani Rizki Amalina Rochmah, Yayun Siti Rochmah S.Pd. M Kes I Ketut Sudiana . Sandy Christiono Sudarsono, Wahyudi Suparmi Syiral Mastura Abdullah Tania Saskianti Teguh Budi Wibowo Udijanto Tedjosasongko Yayun Siti Rochmah Yemy Ameliana, Yemy Yuniar, Savira Nurazky Zurairahbt Ibrahim

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