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All Journal HAYATI Journal of Biosciences Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Jurnal Ilmiah Mahasiswa Kelautan Perikanan Unsyiah Biosaintifika: Journal of Biology & Biology Education Aceh International Journal of Science and Technology Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Aceh Journal of Animal Science Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan
ZAINAL ABIDIN MUCHLISIN
Department Of Aquaculture, Faculty Of Marine And Fisheries, Syiah Kuala University
Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Physics Veterinary

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Application of pumpkin flour to the diet of Barramundi Lates calcarifer fingerling Yanti, Riska Z.; Muchlisin, Zainal A.; Muhammadar, Abdullah A.; Fadli, Nur
Depik Vol 11, No 2 (2022): August 2022
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.11.2.26859

Abstract

BarramundiLates calcariferis a high economic value fish in Indonesia and Southeast Asia countries. However, the aquaculture system still experiences problems, including the high cost of production, specifically from feed, which is due to its relatively slow growth and low digestibility. Therefore, this study aims to determine alternative ingredients that can be used in the fish diet to improve feed utilization and growth performance using pumpkin flourCucurbita moschata. A completely randomized design (CRD) with 6 treatments and 3 replications was adopted in this study. The tested diet was (A) diet without pumpkin flour (control), (B) with 5% (C) 10% (D) 15% (E) 20%, and (F) 25%. The size of the Barramundi samples was 3-4 cm on average and the body weight was 1-3 g at the stocking density of 15 fish/container. The fish fed as much as 10% body weight daily, which is divided into 2 times, namely 08.00 AM and 4.00 PM for 60 days. The results showed that the addition of pumpkin flour had a significant effect on weight gain, daily and specific growth rate, survival, feed efficiency, conversion ratio, and carcass protein (P0.05). The diet with 5% pumpkin flour gave the best results over other treatments, with an average weight gain of 0.761 g, feed conversion of 1.578, feed efficiency of 63.455%, and a daily and specific growth rate of 0.012 g and 0.738%, respectively, The highest carcass protein content was found in the control treatment with a value of 18.210%, which was not significantly different from the 5% pumpkin flour treatment. Therefore, it is concluded that pumpkin flour can be added to the Barramundi diet as much as 5%.Keywords:Barramundi, Snapper, pumpkin,feed utilization
Diversity of commercially important grouper (Family: Epinephelidae) in Simeulue and Banyak Islands, Aceh, Indonesia Razi, Nanda Muhammad; Muchlisin, Zainal A.; Ramadhaniaty, Mutia; Damora, Adrian; Nur, Firman M.; Siti-Azizah, Mohd Nor; Fadli, Nur
Depik Vol 11, No 1 (2022): April 2022
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.11.1.23790

Abstract

Grouper is one of the economically important fish groups in the Simeulue and Banyak Islands. However, data on the diversity of the grouper in this region is still limited. This study aimed to inventory the commercially important grouper in Simeulue and Banyak Islands. Sampling was carried out in April - September 2021 at sixteen locations around Simeulue and Banyak Islands. In total, 20 species of commercial grouper consisting of 6 genera were recorded during the study. Epinephelus was the most dominant genus with ten species, followed by Cephalopholis with four species. In addition, seventeen species (85%) were included in the Least Concern (LC), two species (10%) were categorized as Vulnerable (VU), and one species (5%) was Data Deficient (DD). This study provides a database of commercially important grouper in Simeulue and Banyak Islands that can be used to manage the fish group.
Cellulase activity of bacteria isolated from water of mangrove ecosystem in Aceh Province Dewiyanti, Irma; Darmawi, Darmawi; Muchlisin, Zainal Abidin; Helmi, Teuku Zahrial; Arisa, Iko Imelda; Defira, Cut Nanda; Fitriyani, Fitriyani; Yura, Sawva
Depik Vol 10, No 3 (2021): December 2021
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.10.3.22964

Abstract

Cellulolytic bacteria that produce cellulase enzymes play an essential role in degrading cellulose in their habitat. The presence of cellulolytic bacteria strongly supports the fertility and productivity in mangrove waters. The objectives of the study are to analyze the activity of cellulase enzyme qualitatively through the cellulolytic index and quantitatively through the activity and specific activity of the cellulase enzyme from bacteria isolated from the water of mangrove ecosystems in Aceh Province. The qualitative experiment of enzyme activity was carried out at the Microbiology laboratory SKIPM Aceh, and a quantitative experiment of enzyme activity was conducted at the Microbiology Laboratory, Biology Department, IPB. Isolation of cellulolytic bacteria isolated from mangrove water usedCarboxy Methyl Cellulose(1% CMC) selective media and carried out by spread plate method. The ability of bacteria to produce cellulase was tested qualitatively using the spot technique, this test was carried out using 1% Congo Red. Furthermore, the quantitative testing of cellulase enzymes activity adopted the DNS spectrophotometric method. The specific activity of the cellulase enzyme can be determined by using the Lowry method. There were 21 isolates that had a clear zone and had the ability to produce cellulase enzymes from 49 isolates that were successfully purified. The highest cellulolytic index (CI) produced using BAM421 isolate with the value of 5.50 was included in the high category, followed by BAM326 and BAM132 isolates, with values of 1.55 and 1.05 were categorized into the medium category. The other isolates were in the low cellulolytic index category. The isolate with the highest CI value was further tested using the quantitative enzyme activity test. The highest cellulase enzyme activity of BAM421 occurred at 24hr (0.0029 U/ml). The highest specific cellulase activity of BAM421 was at 24hr with the value of 0.210 U/mg. The result concluded that the qualitative test showed CI values can be categorized into low, medium, and high. Moreover, the value of the quantitative assay described that the cellulase enzyme and the specific enzyme activities of the bacteria were low in the study area.Keywords:Cellulolytic indexQuantitative testMangrove watersCellulase enzymeMicroorganismTRANSLATE with x EnglishArabicHebrewPolishBulgarianHindiPortugueseCatalanHmong DawRomanianChinese SimplifiedHungarianRussianChinese TraditionalIndonesianSlovakCzechItalianSlovenianDanishJapaneseSpanishDutchKlingonSwedishEnglishKoreanThaiEstonianLatvianTurkishFinnishLithuanianUkrainianFrenchMalayUrduGermanMalteseVietnameseGreekNorwegianWelshHaitian CreolePersian// TRANSLATE with COPY THE URL BELOW Back EMBED THE SNIPPET BELOW IN YOUR SITE Enable collaborative features and customize widget: Bing Webmaster PortalBack//
Grouper DNA barcoding studies in Indonesia: A short review Razi, Nanda Muhammad; Muchlisin, Zainal A.; Maulida, Siti; Ramadhaniaty, Mutia; Nur, Firman M.; Damora, Adrian; Buang Manalu, Sumarni Laila; Fadli, Nur
Depik Vol 10, No 2 (2021): August 2021
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.10.2.21255

Abstract

Indonesia is recognized as one of the territories that have the highest reef fish biodiversity in the world. One of the commercially valuable fish in this area is the groupers (locally name "kerapu"). At least 76 grouper species have been reported in Indonesian waters, with three species were categorized into "vulnerable", five species "Data Deficient", and 68 species under the "Least Concern" category based on IUCN classification. The increasing exploitations rate had been reported caused the grouper stocks in Indonesia to decrease and threatened extinction. However, only limited scientific data is available regarding the grouper in Indonesia, including their identification. In most fish landing sites across Indonesia, the groupers are morphologically identified and recorded as "kerapu" to replace their scientific species names. Accurate species identification is essential in designing appropriate and sustainable management of fisheries resources. One of the tools that have been used in fish identification is DNA barcoding. In the last two decades, this molecular method has been applied to identify many fish groups globally, including grouper fish. This study reviewed the DNA barcoding approach in grouper identification in Indonesia based on the available literature.Keywords:DNA barcodingGrouperMolecular TaxonomyFisheriesIndonesia
Critical thermal limit and behavior of the Barramundi (Lates calcarifer Bloch, 1790) fingerling exposure with different temperature Amir, Fachrurozi; Nur, Firman M.; Fadli, Nur; Muchlisin, Zainal Abidin
Depik Vol 10, No 1 (2021): April 2021
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.10.1.20287

Abstract

Barramundi (Lates calcarifer) is one of the commercial fish in Indonesia. The phenomenon of climate change due to global warming has affected the life of aquatic biota, including fish. The objectives of the present study were to determine a critical thermal maximum (CTMax) and the behavior of Barramundi (L. calcarifer) due to the increase in water temperature. The research was conducted at the Fishing Technology Laboratory, Faculty of Marine and Fisheries, Universitas Syiah Kuala in February 2021. The experiment was conducted in 10 aquariums equipped with heaters. The initial temperature of the treatment was 28oC and increased gradually every one minute by 0.2oC. The results showed that the abnormal behavior of Barramundi fingerling was started to appear at 37oC where the fish began to swim fast, then swim sideways at 38oC-39oC, hit the container wall at 39oC-40oC, jumped and weakened then dead at 40oC and 41oC. The average critical temperature of Barramundi fingerling was recorded at a temperature of 41oC within 65 minutes. Therefore, it is concluded that the Barramundi (L. calcarifer) display an abnormal behavior above the temperature of 35oC, however, they can survive up to 42oC exposure for 70 minutes.Keywords:Critical thermal maximumTemperature riseGlobal warming
A short review of discovery and development of fish sperm cryopreservation Afriani, Dian; Eriani, Kartini; Muchlisin, Zainal Abidin; Hasri, Iwan
Depik Vol 10, No 1 (2021): April 2021
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.10.1.18794

Abstract

Global biodiversity, especially fish, has experienced a decline, this occurs as a result of over-exploitation, the presence of introduced fish species and climate change. This condition makes researchers look for solutions to overcome these problems by using cryopreservation techniques. The main purpose of cryopreservation is to store, maintain, and ensure the survival of genetic material, so that using cryopreservation techniques can maintain the viability and function of gamete cells both immunologically, biologically and physiologically. The success of the cryopreservation technique has made this technique widely developed in various species of living organism including fish. This article summarizes and reviews the history of the development of cryopreservation of animal species with specific focus on fish.Keywords:CryopreservationHistoryDepikEndemic species
Co-Authors Abdullah A. Muhammadar Adrian Damora Agus Halim, Agus Aldila, Dhea Febby Alfis Syahrin Amir, Fachrurozi Amirah Hurzaid Amirul Asraf Buang Manalu, Sumarni Laila Chitra Octavina Cut Nanda Defira, Cut Nanda Cut Yulvizar Darmawi . Dian Afriani, Dian Essy Harnelly Faradilla, Suci Budi Fardin Afrido, Fardin Firdus Firdus Fitriana, Mustika Marzah Fitriyani Fitriyani Gheitsa, Tjut N.G. Habib, Ahasan Iko imelda arisa, Iko imelda Irma Dewiyanti Iwan Hasri Iwan Hasri, Iwan Kartini Eriani KARTINI ERIANI Kocabas, Filiz Kutluyer Mahfud Mahfud Mahfudhah, Raudhah Maulida, Siti Mohd N. Siti-Azizah Muhammad Nasir Muhammadar, Abdullah Abbas Nanda Muhammad Razi Nasrul Arahman Nur Asma Nur Fadli Nur, Firman M. Nurlaili Nurlaili Purwana Satriyo Qurrata Akyun, Qurrata Radhi, M Rahayu, Sri Riska Ramadhaniaty, Mutia SALMA, ITSNATANI Sarah, Putri Inten Satria Rizka, Satria Setiawan, Diky Agung Siregar, Lisna Yanti Siti-Azizah, Mohd Nor Sri Jumiati, Sri Syahril, Alfis Tanzil Murda, Tanzil Teuku Zahrial Helmi Yanti, Riska Z. Yura, Sawva Zulkarnain Jalil