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Pengaruh Lama Fermentasi Dedak Gandum Terhadap Kandungan Serat Kasar, NDF dan ADF Mesang, Sarintan Novite I.; Oematan, Gustaf; Mullik, Marthen L.; Dami Dato, Twen O
Animal Agricultura Vol 2 No 2 (2024): Animal Agricultura
Publisher : Yayasan Sumber Daya Manusia Cerdas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59891/animacultura.v2i2.84

Abstract

This study aims to determine the effect of the duration of wheat bran fermentation on the content of crude fiber, neutral detergent fiber (NDF), and acid detergent fiber (ADF). The design used was the Completely Randomized Design (CRD) method with 4 treatments and 4 replications, resulting in 16 experimental units. The treatments consisted of fermentation durations of 0 days (LF0), 2 days (LF2), 4 days (LF4), and 6 days (LF6). Prior to fermentation, wheat bran was mixed with an inoculum solution at a 1:1 ratio. The inoculum was made from a mixture of 16 liters of clean water, 30 ml of EM4, 100 ml of liquid sugar, and 100 ml of fresh cow feces liquid taken from cows fed with lamtoro and fresh grass. The variables studied in this research were the content of crude fiber, neutral detergent fiber (NDF), and acid detergent fiber (ADF). The data were analyzed using analysis of variance (ANOVA) for CRD where the alpha value was set at 0.5%, and differences between treatments were followed by Duncan's test using SPSS version 25. The results of the statistical analysis showed that the duration of wheat bran fermentation had a very significant effect (P<0.01) in reducing all fiber variables. The total value of crude fiber content decreased from 51.95% (LF0) to 42.86% (LF6), likewise NDF decreased from 36.55% (LF0) to 29.90% (LF6), and ADF content decreased from 13.62% (LF0) to 9.12% (LF6). Based on the results of this study, it can be concluded that the longer fermentation of wheat bran using fresh cow feces liquid as an inoculum is capable of reducing the content of crude fiber, NDF, and ADF with a fermentation duration of 6 days (LF6), obtaining total values of crude fiber content (42.86%), NDF (29.90%), and ADF (9.12%).
Pengaruh Lama Fermentasi Dedak Gandum Terhadap Kandungan Asam Fitat Serta Kecernaan In Vitro Bahan Kering dan Bahan Organik Mbali, Rambu Yow; Mullik, Marthen L.; Oematan, Gustaf
Animal Agricultura Vol 2 No 2 (2024): Animal Agricultura
Publisher : Yayasan Sumber Daya Manusia Cerdas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59891/animacultura.v2i2.86

Abstract

This study aimed to determine the effect of the duration of wheat bran fermentation on phytate acid content, in vitro dry matter digestibility, and organic matter digestibility. The method used in this study was a Completely Randomized Design (CRD) consisting of 4 treatments and 4 replications, resulting in 16 experimental units. The treatments were as follows: LF0: without fermentation (control), LF2: fermentation for 2 days, LF4: fermentation for 4 days, and LF6: fermentation for 6 days. The mixture of the research material before fermentation was wheat bran mixed with an inoculum solution at a ratio of 1:1. The inoculum was made from a mixture of 15 liters of clean water, 30 ml of EM4, 100 ml of liquid palm sugar, and 100 ml of fresh cow dung fluid taken from cows fed with fresh leucaena leaves and grass. The variables measured in this study were phytate acid content, dry matter digestibility (DMD), and organic matter digestibility (OMD). The results of the statistical analysis showed that the treatments had a very significant effect (P=0.01) on the phytate acid content, dry matter digestibility (DMD), and organic matter digestibility (OMD) of fermented wheat bran. The total phytate acid content value in LF0 was 0.48%, which decreased to 0.28% in LF6, dry matter digestibility in LF0 was 69.75% and increased to 74.98% in LF2, and organic matter digestibility in LF0 was 70.19% and increased to 75.56% in LF6. Based on the research results, it can be concluded that wheat bran with different fermentation durations using fresh cow dung can reduce phytate acid content and increase dry matter digestibility and organic matter digestibility in wheat bran.
Pengaruh Lama Waktu Biofermentasi Chromolaena Odorata dengan Sumber Karbon Tepung Putak terhadap Kandungan Serta Kecernaan Protein Kasar dan Lemak Kasar In vitro Nomleni, Meri; Dami Dato, Twen O.; Lestari, Gusti A. Y.; Mullik, Marthen L.
Animal Agricultura Vol 2 No 3 (2025): Animal Agricultura
Publisher : Yayasan Sumber Daya Manusia Cerdas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59891/animacultura.v2i3.108

Abstract

The purpose of this study was to determine the effect of the length of biofermentation time of chromolena odorata with a carbon source of putak flour on the content and digestibility of crude protein and crude fat in vitro. The research method used is an experimental method (experimental) with a Complete Random Design pattern consisting of 4 treatments and 4 repeats so that there are 16 experimental units. The treatment used is LB21: biofermentation duration 21 days (As control), LB14: biofermentation duration 14 days, LB7: biofermentation duration 7 days, LB0: biofermentation duration 0 days. The data were obtained and analyzed by variety analysis (ANOVA), and continued with the Duncan multiple distance test to determine the effect between treatments. The results of statistical analysis showed that the length of biofermentation of Chromolaena odorata with the carbon source of putak  flour had a real effect (P<0,01) On crude protein content, crude fat content and crude protein digestibility in vitro, while on crude fat digestibility in vitro the effect is not real (P<0,05). It can be concluded that the length of biofermnetation of Chromolaena odorata with putak flour source affects crude protein content, crude fat and gauze protein digestibility in vitro but does not affect crude fat digestibility in vitro.  
Pengaruh Skarifikasi Benih terhadap Pertumbuhan Tanaman Mucuna bracteata: Effect of Seed Scarification on the Growth of Mucuna bracteata Plants Darmin, Vinensius; Mullik, Marthen L.; Dami Dato, Twen O.
Jurnal Ilmu Nutrisi dan Teknologi Pakan Vol. 23 No. 2 (2025): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jintp.23.2.%p

Abstract

The main problem in the cultivation of Mucuna bracteata is the hardness of the seed coat, which results in low seed dormancy. The aim of this study was to examine the effectiveness of different seed scarification methods on the growth of M. bracteata plants. This research was conducted at Prof. Bale-Therik's farm in Noelbaki Village, Kupang Tengah Subdistrict, Kupang Regency, for 21 days. This research used a completely randomized design (CRD) of 4 treatments and 5 replicates, with each replicate consisting of 15 seeds. The treatments consisted of control (SB0); seeds soaked in warm water at 50°C for 2 hours (SB1); seeds soaked in normal temperature water for 24 hours (SB2); and seeds scarified (SB3). Data obtained during the study were analyzed using analysis of variance to test the effect of treatment with an alpha value of 0.05. Differences between treatments were tested by Duncan's multiple range test. The results showed that seed scarification had a very significant effect (p<0.01) on all parameters measured. SB3 treatment significantly accelerated leaf emergence (6.21 days), number of leaves (27.47 strands), leaf length (15.51 cm), leaf width (9.89 cm), the onset of tendril growth (8.33 days), tendril length (117.16 cm), and tendril growth rate (6.89 cm day-1). It was concluded that the seed scarification technique (SB3) was the best treatment because it could influence and improve the growth of M. bracteata plants. Keywords:           dormancy, growth, Mucuna bracteata, scarification
Pengaruh Lama Waktu Biofermentasi Chromolaena odorata dengan Penambahan Sumber Karbon Gula Lontar Cair terhadap Kandungan Selulosa, Lignin, Asam Pitat, Kadar Nitrit dan Saponin Lie, Cinta Kristin; Mullik, Marthen L.; Dami Dato, Twen O.; Oematan, Gustaf
Animal Agricultura Vol 2 No 1 (2024): Animal Agricultura
Publisher : Yayasan Sumber Daya Manusia Cerdas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59891/animacultura.v2i1.66

Abstract

The purpose of this study was to determine the effect of the addition of liquid palm sugar as a carbon source in the Chromolaena odorata biofermentation process on cellulose, lignin, phytic acid, nitrite and saponin content. This study used a completely randomized design (CRD) with 4 treatments and 4 replicates. The treatments were: LB0 = 0 days of biofermentation, LB7 = 7 days of biofermentation, LB14 = 14 days of biofermentation, and LB21 = 21 days of biofermentation. The variables observed were cellulose, lignin, phytic acid, nitrite and saponin content. The data obtained were analyzed using Analysis of Variance and Duncan's test. The results showed that the length of biofermentation time significantly decreased cellulose content with a range of (23.52-19.26%), lignin (13.75-8.72%), phytic acid (4.03-2.00%), nitrite content (6.60-4.73ppm), and saponin (7.68-5.84%). It was concluded that the length of Chromolaena odorata biofermentation time decreased the content of cellulose, lignin, phytic acid, nitrite content and saponins with varying lengths of biofermentation time, namely the length of biofermentation time of 7 days (LB7) getting a total value of cellulose content (20, 40%), lignin (9.23%), the length of biofermentation time of 14 days get the total value of phytic acid content (2.23%), nitrite (4.86ppm) and the length of biofermentation time of 21 days get the total value of saponin content (5.84%).
Pengaruh Lama Waktu Biofermentasi Chromolaena odorata dengan Sumber Karbon Gula Lontar Cair terhadap Kandungan Energi Leki, Sindi Rambu; Dami Dato, Twen O; Mullik, Marthen Luther; Benu, Imanuel
Animal Agricultura Vol 2 No 1 (2024): Animal Agricultura
Publisher : Yayasan Sumber Daya Manusia Cerdas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59891/animacultura.v2i1.70

Abstract

This study aims to determine the duration of Chromolaena odorata biofermentation with liquid palm sugar carbon source on energy content. The method used in this research is experimental method to test 4 treatments of fermentation time on Chromolaena odorata. The research design used was a completely randomized design (CRD) with 4 treatments and 4 replicates so that there were 16 experimental units. The treatments were: LB-0 = without biofermentation, LB-7 = 7 days biofermentation duration, LB-14 = 14 days biofermentation duration, LB-21 = 21 days biofermentation duration. Variables observed were carbohydrate content, BETN, gross energy, metabolic energy and TDN. Data were analyzed by variance analysis at 5% alpha level, differences between treatments were tested by Duncan's multiple tests. The results showed that the duration of Chromolaena odorata biofermentation with liquid palm sugar carbon source had a very significant effect (P=0.001) on BETN content (37.94-43.89%), GE content (4,282-4,429 kcal/kg BK), and EM content (3,067-3,443 kcal/kg BK), while the carbohydrate content (60.02-62.69%) and TDN content (61.60-63.80%) had no significant effect (P=0.07 and 0.27). It was concluded that the length of time of Chromolaena odorata biofermentation with liquid palm sugar carbon source increased energy content, with the best length of time for carbohydrate, BETN, and TDN for 7 days; GE for 14 days, while EM for 21 days.
Improving Feed Intake, Digestibility, Rumen Fermentation, and Blood Profiles in Kacang Goats through Pueraria phaseoloides Supplementation in Kume Grass Hay Diets Benu, I.; Jelantik, I. G. N.; Mullik, M. L.; Malelak, G. E. M.; Oematan, G.; Laut, M. M.
Tropical Animal Science Journal Vol. 47 No. 1 (2024): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2024.47.1.79

Abstract

This study was conducted to investigate the effects of supplementing Pueraria phaseoloides on feed intake, nutrient digestibility, rumen fermentation, and blood profiles in male Kacang goats fed Kume grass hay as a basal diet. Twelve male Kacang goats aged between 6 and 8 months with a mean body weight of 13.63 ± 1.40 kg were randomly assigned to four dietary treatments (three goats per treatment) in a 3x4 incomplete Latin square design. The treatments consist of a control group (P0) receiving grass hay and concentrate and three groups supplemented with 10% (P10), 20% (P20), and 30% (P30) P. phaseoloides of protein requirement on a dry matter basis. The data obtained in this study were statistically analyzed using the GLM procedure following ANOVA in SPSS Statistics for Windows, version 22. The results indicated that P. phaseoloides significantly (p<0.05) increased feed intake, with goats receiving P. phaseoloides consuming more dry matter compared to the control group (P0). Furthermore, nutrient digestibility improved (p<0.05) with P. phaseoloides supplementation. However, rumen fermentation characteristics, including NH3-N concentration, VFA concentration, and ruminal pH, did not differ significantly among the dietary treatments. Additionally, there were no significant differences in blood profiles among the Kacang goats under different dietary treatments. In conclusion, supplementation of P. phaseoloides in male Kacang goats fed Kume grass hay as a basal diet improved feed intake and nutrient digestibility but had no significant effect on rumen fermentation or blood profiles. Therefore, P. phaseoloides can be used as a feed supplement for ruminants consuming low-quality grass.
Improving the Rumen Molar Proportion of Glucogenic Volatile Fatty Acids with the Inclusion of Siam Weed (Chromolaena odorata) Meal in Pelleted Diet of Fattened Cattle Mullik, M. L.; Dato, T. O. D.; Mulik, Y. M.; Oematan, G.
Tropical Animal Science Journal Vol. 47 No. 1 (2024): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2024.47.1.97

Abstract

The objective of this metabolism study was to evaluate the efficacy of Siam weed (Chromolaena odorata) meal in pelleted diets for fattened cattle. Four 2-year-old Bali steers were assigned to four dietary treatments using a 4x4 Latin square experimental design. The treatments were pellets containing chromolaena meal at 10% (10COM), 20% (20COM), 30% (30COM), or 40% (40COM). The pellet was given at 2% liveweight (LW), and kume (Sorghum plumosum var. Timorense) grass hay was offered ad libitum. The diets were isonitrogenous (20%) and energy (11.5 MJ ME/kg DM). Dietary intake, digestibility, and rumen fermentation were the variables measured. The results showed that increasing chromolaena meal to 40% substantially decreased the nutrient intake. Dry matter intake decreased from 2.5% LW in the 10COM to 2.19% LW in the 40COM. Likewise, crude protein intake decreased from 749 g/d (10COM) to 661 g/d (40COM). On the contrary, digestibility, rumen pH, ammonia concentration (116–125 mg/dL), and volatile fatty acids were not affected. It might be concluded that chromolaena can be used as a protein source for ruminants, but at high levels of inclusion (40%) tends to reduce intake.
Cholesterol and Blood Profile of Bali Cattle Fed Chromolaena odorata Weed with Rice Straw as Basal Feed Oematan, Gustaf; Mullik, Marthen Luther; Benu, Imanuel; Jelantik, I Gusti Ngurah; Dato, Twenfosel Dami; Lestari, Gusti Ayu Yudit; Malelak, Gemini Ermiani; Hartati, Erna; Lazarus, Edwin; Yunus, Marten
Jurnal Penelitian Pendidikan IPA Vol 10 No 4 (2024): April
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i4.5263

Abstract

This study aims to determine the effect of the level of C. odorata (CO) silage-based concentrate on the blood profiles of Bali cattle. Three male Bali cattle (initial 2 years old with CV = 19,28%) were randomly assigned to one of three meals: rice straw and concentrates containing C.odorata silage 30% (RCO30) or  C.odorata silage 40% (RCO40) or C.odorata silage 50% (RCO50), respectively, using a 3 x 3 Latin Square Design. The was no significant effect between treatments (P> 0.05) on blood cholesterol (195.74-220.49 mg/dL), blood urea (48.41-49.44 mg/dL), total blood plasma (6.08-6.93 g/dL), blood haemoglobin (Hb; 18.18-18.92 g/dL), hematocrit levels (45.56-53.03%), erythrocytes (7.06-8.24 106/l), and leucocytes (8.56-8.84/l). Blood glucose levels, however, differed significantly (P<0.05) between treatment. Feeding Bali cattle with different levels of C.odorata-based concentrate resulted in the same effect on all blood parameters except blood glucose. Therefore, it is recommended that the use of C.odorata in a ration of Bali cattle can be up to 50%.
Pengaruh Level Inokulum Cairan Rumen Sapi Terhadap Kandungan Serta Kecernaan in vitro Bahan Kering dan Bahan Organik Kulit Kopi Fermentasi Nobertus Gibrandi Ampur; Twenfosel O. Dami Dato; Marthen L. Mullik
Journal of Innovative and Creativity Vol. 5 No. 3 (2025)
Publisher : Fakultas Ilmu Pendidikan Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/joecy.v5i3.4081

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan inokulum cairan rumen sapi pada berbagai level terhadap kandungan serta kecernaan in vitro bahan kering dan bahan organik kulit kopi fermentasi. Penelitian ini menggunakan rancangan acak lengkap (RAL) terdiri dari 5 perlakuan dan 4 ulangan, total 20 unit sampel percobaan, di mana setiap unit percobaan menggunakan 1 kg kulit kopi yang ditambahkan gula cair sebanding dengan cairan rumen, total kulit kopi yang digunakan sebanyak 20 kg. Kelima perlakuan tersebut adalah LI-0 = kulit kopi tanpa inokulum, LI-10 = kulit kopi+10 ml inokulum, LI-20 = kulit kopi+20 ml inokulum, LI-30 = kulit kopi+30 ml inokulum, LI-40 = kulit kopi+40 ml inokulum. Parameter yang diteliti meliputi kandungan dan kecernaan in vitro bahan kering serta bahan organik. Hasil penelitian menunjukkan bahwa level inokulum cairan rumen sapi pada fermentasi kulit kopi memberikan pengaruh nyata (P<0,05) terhadap kandungan bahan kering dan kecernaan in vitro bahan kering, namun tidak memberikan pengaruh nyata (P>0,05) terhadap kandungan bahan organik dan kecernaan in vitro bahan organik. Dari hasil tersebut dapat disimpulkan bahwa cairan rumen sapi dapat digunakan sebagai starter inokulum dalam fermentasi limbah kulit kopi hingga level 40 ml/kg substrat berdasarkan kandungan dan kecernaan in vitro bahan organik.