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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) AGRIVITA, Journal of Agricultural Science BERITA BIOLOGI JURNAL BIOLOGI INDONESIA ANNALES BOGORIENSES Biosaintifika: Journal of Biology & Biology Education Journal of Tropical Crop Science AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Biofarm : Jurnal Ilmiah Pertanian Annales Bogorienses
Enung Sri Mulyaningsih
Research Center for Biotechnology, Lembaga Ilmu Pengetahuan Indonesia, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Environmental Science Immunology & microbiology Medicine & Pharmacology

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Parameter Genetik Karakter Agronomi pada Galur F1 Padi Hasil Persilangan Galur Murni dan Kultivar Lokal Indonesia Adi, Eko Binnaryo Mei; Indrayani, Sri; Burhana, Nana; Mulyaningsih, Enung Sri
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 5 No 1 (2021): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/agrosainstek.v5i1.143

Abstract

Rice is one of the main sources of carbohydrates for Indonesian society. The development of new varieties depends on the availability of germplasm as the source genetic material. The development of new varieties can be done through artificial crosses (local cultivars and superior lines) for the creation of diversities. The research aimed to explore the genetics parameters from eight rice characters, for the selection program. The crossing was conducted by using four local cultivars and one pure line with diverse agronomic characters. Four hybrids (F1) were planted using completely randomized design with four replications, and five parental as the check varieties (landrace and pure line). The correlation coefficient was found that there was one character showed strong correlation almost all the character that is panicle weight. While high heritability was found in harvest age, panicle length, number of grain per panicle, 1000 grain weight, and plant high. Hybrids that showed negative heterosis for harvest age are from B14081H-296 x Salak and Dampak x Carogol, which have shorter harvest age compared to the average of the parental plant which is dominant gene action and additive.
Tissue Culture Initiation Using Local Maize Explants from the Field Ningsih, Irnawati; Wahyuni, Febriana Dwi; Rahman, Nurhamidar; Fitriani, Hani; Rianawati, Sri; Supriadi, Dedi; Mulyaningsih, Enung Sri
Biofarm : Jurnal Ilmiah Pertanian Vol. 20 No. 2 (2024): BIOFARM JURNAL ILMIAH PERTANIAN
Publisher : Universitas Pekalongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31941/biofarm.v20i2.5019

Abstract

Inisiasi kultur jaringan adalah salah satu metode bioteknologi yang bertujuan untuk menghasilkan tanaman baru menggunakan eksplan yang telah ada. Teknik kultur jaringan saat ini telah berkembang perannya sebagai tool untuk menghasilkan tanaman hasil pemuliaan modern seperti hasil rekayasa. Namun untuk dapat dimanfaatkan sebagai tool, penguasaan kultur jaringan di tahap awal (inisiasi) perlu dikuasai. Jagung lokal yang berasal dari lapangan merupakan salah satu eksplan yang dapat dimanfaatkan untuk kegiatan inisiasi kultur jaringan. Hal ini menarik dilakukan karena jagung merupakan tanaman graminea yang paling rekalsitran terhadap kultur jaringan. Kegiatan dimulai dengan memilih varietas jagung lokal, menggunakan material genetik berupa benih tua (jagung pipilan) kemudian dilakukanlah optimasi proses sterilisasi. Teknik sterilisasi yang digunakan bersifat fast dan overnight. Benih yang telah steril ditumbuhkan pada media dasar. Selanjutnya untuk percobaan kultur jaringan, material genetik dibedakan atas bagian: buku utuh, buku dibelah dan ujung akar. Material eksplan diperoleh dari seedling yang tumbuh steril dan normal pada hasil percobaan sterilisasi. Eksplan yang diperoleh di tanam pada media NB, 1⁄2 MS, dan SH yang mengandung kombinasi zat pengatur tumbuh (ZPT) yaitu: label A) TDZ 0,75 mg/L (Thidiazuron) & BAP 0,25 mg/L (Benzyl amino purin), label B) dengan hormon NAA 0,5 mg/L (Naptalaneacetic acid) & BAP 1 mg/L (Benzyl amino purin), dan label C) dengan hormon 2,4-Dichlorophenoxy Acid 1 mg/L dan Kinetin 0,5 mg/L yang dilengkapi asam amino (proline, casein) sumber karbon (sukrosa), dan pemadat (phytagel). Hasil penelitian ini didapatkan bahwa dengan sterilisasi cepat, pertumbuhan tanaman memiliki hasil perkembangan yang cepat yaitu tumbuh pada hari ke 10, namun memiliki tingkat kontaminasi yang tinggi. Sedangkan dengan sterilisasi overnight memiliki hasil perkembangan yang lambat yaitu tumbuh pada hari ke 16, namun memiliki tingkat kontaminasi yang rendah. Kombinasi ZPT dengan media yang paling cocok adalah media NB yang dikombinasikan dengan ZPT TDZ 0,75 mg/L dan BAP 0,25 mg/L, menghasilkan plantlet dari bagian buku, dan kalus dari bagian ujung akar, walaupun kalus yang di maksud masih kalus secara umum. Penyimpanan pada lingkungan gelap, memiliki persentase pertumbuhan yang lebih cepat jika dibandingkan dengan perlakuan penyimpanan pada lingkungan terang. Dan hasil akhir juga menunjukan bahwa pada kondisi terang, tingkat dorman/ mati nya eksplan lebih tinggi.
Grafting-Induced Phenotypic and Genomic Variations in Cassava (Manihot esculenta Crantz) through Interspecific Compatibility with M. Glaziovi Ulfa, Umi Tahniah; Sudarsono, Sudarsono; Ardie, Sintho Wahyuning; Mulyaningsih, Enung Sri; Hartati, N. Sri; Anjarsari, Nurul Aeni; Dewi, Prita Sari
Journal of Tropical Crop Science Vol. 12 No. 03 (2025): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.12.03.559-571

Abstract

Grafting is a promising approach to enhance cassava (Manihot esculenta Crantz) productivity, particularly in improving tuber yield and quality. Despite its technical simplicity, research on the morphological and molecular consequences of grafting in cassava is limited. This study aimed to quantitatively assess phenotypic differences between grafted and shoot cutting plants and characterize genetic modifications in shoot apical meristems near the graft union. Three splice-grafting combinations were evaluated: M. glaziovii (cv. "Karet")/"Revita RV1" (KR), "Karet"/ "Carvita 25" (KC), and "Carvita 25"/ "Revita RV1" (CR), alongside shoot-cutting plants of each cultivar. Grafted seedlings and conventional cuttings were transplanted to the field, and growth was monitored up to five months after grafting (5 MAG). Shoot bud samples were collected for RAPD analysis at three months (3 MAG). Grafted plants showed differences in morphology compared to controls, especially in branching architecture, branch angle, stem diameter, and internode length. However, the overall plant form and primary stem type remained stable. The color of emerging shoots consistently reflected their genotypic origin, like scion or rootstock, as did the shoot buds nearest the graft union. RAPD analysis using multiple primers revealed polymorphic banding patterns, with OPE-12 producing the most diverse profiles. While specific bands were associated with each cultivar, grafted regenerants often exhibited altered or novel band profiles, suggesting somaclonal variation or graft-induced genetic/epigenetic changes. Some regenerants displayed unexpected band types, such as C1 in R-KR1 and R-KR3, which were absent from their known parental genotypes. Cluster analysis grouped 45 samples into five major clusters and eight subgroups, indicating genetic divergence among certain grafted combinations, particularly "Carvita 25"–"Revita RV1" and M. glaziovii–"Revita RV1". These results demonstrate that grafting can significantly impact cassava phenotype and genome stability. This study shows that interspecific grafting between cassava (Manihot esculenta Crantz) and M. glaziovii can induce significant phenotypic and genomic variations.
Transformasi Padi Indica Kultivar Batutegi dan Kasalath dengan Gen Regulator HD-Zip untuk Perakitan Varietas Toleran Kekeringan Mulyaningsih, Enung Sri; Aswidinnoor, Hajrial; Sopandie, Didy; Ouwerkerk, Pieter B.F.; Loedin, Inez Hortense Slamet
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 1 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (348.06 KB) | DOI: 10.24831/jai.v38i1.1676

Abstract

water deficiency. Genetic engineering at the level of transcription factors (TF) is particulary a promising strategy in developing drought tolerant rice cultivar. HD-Zip genes are TF that function in plant adaptation to some environmental stresses including water deficit. The recombinant plasmid pC1301H Oshox6 which contained HD-Zip Oshox6 gene was placed under a drought inducible promoter called LEA promoter, gusA and hpt genes were driven with CaMV promoter. The aim of research was to obtain indica rice transgenic plants of Batutegi and Kasalath cultivars using pC1301H Oshox6 plasmid. Recombinant plasmid was transformed into immature rice embryos using Agrobacterium tumefaciens. Kasalath cultivar showed a better capacity to form embryogenic calli compared to Batutegi. Transformation efficiency of Batutegi is lower (1.5 - 0.3%) than Kasalath (2.2-28.3%). Regeneration efficiency is 25-83.3% and 7.7-100% for Batutegi and Kasalath, respectively. Number of putative transformant plantlets of Batutegi and Kasalath are 63 and 48 plantlets, respectively. Southern blot analysis (using hpt probe) on 12 independent lines of each Batutegi and Kasalath cultivars showed different gene copy number, ranging from one to four copies of gene.   Keywords: agrobacterium tumefaciens, LEA promoter, HD-Zip Oshox6, rice
Phenotypic Screening of Ds Transposon and Activation-tag Insertional Mutant Rice Population for Drought and Salinity Tolerant Related Traits Nugroho, Satya; Zannati, Anky; Situmorang, Apriadi; Windiastri, Vincentia Esti; Widyajayanti, Dwi; Pantouw, Carla Frieda; Astuti, Dwi; Indrayani, Sri; Rachmawati, Syamsidah; Mulyaningsih, Enung Sri; Santoso, Tri Joko; Trijatmiko, Kurniawan Rudi
Annales Bogorienses Vol. 15 No. 1 (2011): Annales Bogorienses
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The studies of rice genes through functional genomics are greatly facilitated by the availability of the complete genome sequences, including the complete physical map of the japonica rice cv. nipponbare. Using the Ac and Ds transposon, that are capable of transposition in various heterologous plants including the monocotyledon rice, combined with the enhancer element for generating activation taged lines, it is possible to discover and isolate functional genes involved in various important agronomical traits; such as those involved in abiotic stress tolerance (drought, high salt) and biotic stresses diseases and pests. We have developed 1,785 first (T0) generation of mutant nipponbare rice by transposons Ac/Ds insertions containing activation-tag, generated by transformation using Agrobacterium-mediated method. Currently, we have generated approximately 1,000 stable lines with transposon Ds and activation-tag insertion ready for screening. Efficient screening methods for mutant Nipponbare rice lines have been established for agronomically important mutant traits. Among the new phenotypes related to important agronomical traits observed were drought and salt tolerant or sensitive, stunted and robust growth, variable root penetration and other interesting traits such as reduced tillering, rolled leaf and thin tiller. 
Co-Authors Adi, Eko Binnaryo Mei Agus Arfani, Agus Ambar Yuswi Perdani, Ambar Yuswi Anggraheni, Yuliana Galih Dyan Anggraheni, Yuliana Galih Dyan Anjarsari, Nurul Aeni Anky Zannati Apriadi Situmorang Burhana, Nana Dedi Supriadi Didy Sopandie Dody Priadi Dwi Astuti Dwi Widyajayantie Febriana Dwi Wahyuni Fiqolbi Nuro Pohan, Fiqolbi Nuro HAJRIAL ASWIDINNOOR Hani Fitriani, Hani Inez Hortense Slamet Loedin IWAN SASKIAWAN Kurniawan Rudi Trijatmiko, Kurniawan Rudi N. Sri Hartati, N. Sri Ningsih, Irnawati OuwerkerV, Pieter BF Pantouw, Carla F. Pantouw, Carla Frieda Pieter B.F. Ouwerkerk Prita Sari Dewi Rachmawati, Syamsidah Rahman, Nurhamidar Satya Nugroho Sintho Wahyuning Ardie Slamet Loedin, Inez Hortense Sri Indrayani, Sri Sri Rianawati Sudarsono Syamsidah Rahmawati, Syamsidah TRI JOKO SANTOSO Ulfa, Umi Tahniah Vincentia Esti Windiastri, Vincentia Esti Widyajayanti, Dwi Yuli Sulistyowati