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Optimasi Konsentrasi Primer Dan Suhu Annealing Dalam Mendeteksi Gen Blaz Pada Bakteri Methicillin Resistant Staphylococcus Aureus Di Udara: Optimization of Primary Concentration and Annealing Temperature in Detecting Blaz Gene in Airborne Methicillin-resistant Staphylococcus aureus Bacteria Mardiana, Anisah; Dhanti, Kurnia Ritma; Kurniawan, Kurniawan; Sulistyowati, Retno
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 1 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i1.6076

Staphylococcus aureus is a species of bacteria that is often identified as a source of infection and has increased the number of cases of resistance. Resistance to Penicillin-class antibiotics in S. aureus is associated with plasmids containing the blah-Z gene encoding ß-Lactamase. Some factors that can be modified to obtain optimal test results in PCR are annealing temperature and primary concentration. This study aims to identify the primary concentration and annealing temperature that produce PCR products with the best band in detecting the blaZ gene in Metichilin Resistant Staphylococcus aureus (MRSA) bacteria. The research design used used experimental methods. The results of the study obtained were PCR products at primary concentrations of 10 μM were clearly visible, thick and clean, compared to primary concentrations of 5 μM. Annealing temperature with the best band results at temperatures of 61.8 °C and 58.7°C. This study concluded the primary concentration to obtain a clearly visible band result of 10 μM. The annealing temperature of 61.8°C produced the best band in the sample code R.PG. U4, R.V.U2, and R.151.U2 temperatures. While the sample code R.IGD. U5 band results best at annealing temperature 58.7°C.

Pembuatan Sediaan Gel Arang Tempurung Kelapa Dan Uji Daya Hambat Terhadap Bakteri Klebsiella pneumoniae: Preparation of Coconut Shell Charcoal Gel and Inhibitory Test Against Klebsiella pneumoniae Bacteria Kamalah, Siti Rahmah; Kurniawan, Kurniawan; Mulyanto, Arif; Dhanti, Kurnia Ritma
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 1 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i1.6082

Klebsiella pneumoniae is a gram-negative rod-shaped bacterium that is a member of the Enterobacteriaceae group of bacteria and is pathogenic and can infect humans. Currently, many K. pneumoniae bacteria are resistant to antibiotics, giving rise to new problems in the health sector that must be addressed immediately by utilizing biodiversity in the form of medicinal plants such as coconut trees, which can produce coconut shells that can be made into charcoal. The results of the phytochemical analysis show that coconut shell charcoal contains many active compounds that have been used in the cosmetic and health industries. The purpose of this study was to obtain an optimal and effective coconut shell charcoal extract gel formula for inhibiting the growth of K. pneumoniae bacteria. This study included a true experimental post-only control group with different gel concentration groups, namely 3%, 6%, and 9%. The optimal formulation of coconut shell charcoal extract gel is a gel with a concentration of 9%, and the results of the one-way ANOVA statistical analysis show that the treatment of the inhibition test on K. pneumoniae bacteria from gels with different concentrations shows that there is a mean difference in the area of the inhibition zone with a significant value of 0.033 (p<0.05). The results of the LSD post-hoc test showed that several treatment groups had significant values (p <0.05). Based on the results of the study, it can be concluded that the optimal formulation of shell charcoal extract gel is a gel with a concentration of 9%, while the most effective gel for inhibiting the growth of K. pneumoniae bacteria is a gel with a concentration of 3%.

Optimasi Suhu Annealing Gen Blaz Dari Bakteri Methicillin-Resistant Staphylococcus Aureus (MRSA) Pada Peralatan Medis: Optimization Annealing Temperature Gene blaZ of Bacterial Methicillin-resistant Staphylococcus aureus (MRSA) in Medical Equipment Listiani, Lia; Dhanti, Kurnia Ritma; Kurniawan, Kurniawan; Widodo, Oei Stefani Yuanita
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 1 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i1.6083

Methicillin-resistant Staphylococcus aureus is a strain of S. aureus bacteria that is resistant to methicillin-type penicillins. MRSA bacteria can cause nosocomial infections, which can be spread through medical equipment. One of the genes that encodes the resistance trait of S. aureus is the blaZ gene. The detection of the blaZ gene requires optimizing the annealing temperature of the primer used to obtain the optimal PCR band. This study aims to optimize the annealing temperature for the amplification of the blaZ gene DNA from Methicillin-resistant Staphylococcus aureus (MRSA) bacteria on medical devices. The method used in this study used a kit for bacterial extraction and Polymerase Chain Reaction(PCR). The results of the blaZ gene DNA amplification in all samples showed clear bands at temperatures of 52.0°C, 54.2°C, 58.7°C, 61.8°C, and 64.0°C; no smears were formed, and the electrophoretic visualization results on the PCR product showed the results of bands that were according to the target, namely 173 bp. The most optimal annealing temperature for amplification of the blaZ gene in MRSA bacteria from hospital medical equipment is 61.8°C.

Surveillance of pathogenic Leptospira among rodents and small mammals in enzootic areas of plague in Pasuruan Indonesia Febriani, Siti Amanah; Dhanti, Kurnia Ritma; Kurniawan, Kurniawan; Ristiyanto, Ristiyanto; Junaedi, Arief; Sukowati, Caecilia Hapsari Ceriapuri; Handayani, Farida Dwi
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 15, No 1 (2024): June 2024
Publisher : Faculty of Medicine, Universitas Islam Sultan Agung (UNISSULA), Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/sainsmed.v15i1.37499

Leptospirosis is a globally significant yet often overlooked zoonotic infection. Several regions in Indonesia are endemic for leptospirosis, including East Java. Pasuruan a region located in the East Java province of Indonesia, has been recognized as an enzootic area of plague infection. However, the presence of other rodent-borne diseases has not been reported. This study aims to detect the presence of pathogenic Leptospira in rats and small mammals in the enzootic plague area of Pasuruan in Indonesia. This study belongs to the type of observational analytic research with a cross-sectional study design. Simple random sampling was conducted to trap a total of 119 forms 107 rats and 12 small mammal kidneys were analyzed molecularly (PCR) to detect Leptospira bacteria with the LipL32 gene as the target. The study revealed that 7.6% (9/119) of rats in the enzootic areas of plague (Sedaeng, Tosari, Surorowo, Petren, Pakis Bincil, Kutukan) were found to carry Leptospira DNA, indicating a significant difference between infection rates per region (p < 0.0001). Rattus tanezumi was identified as the Leptospirosis reservoir in settlements habitats with a percentage of 13.2%, Rattus tiomanicus was detected at 28.6% in forest habitats, and Rattus exulans was found at 4.4% in both habitats. The findings in our study, it is crucial for the community to increase awareness of the spread of rodent-borne diseases, including Leptospirosis.

The effect of a combination of kepok banana peel (musa acuminata balbisiana colla) and bay leaf (syzygium polyanthum) extracts on bleeding time in wistar rats (rattus norvegicus) Sarinastiti, Deliza Dhiakhalda; Sudarsono, Tantri Analisawati; Dhanti, Kurnia Ritma; Wijayanti, Linda; Muniroh, Nur An-Nuha; Cahyaningrum, Winda Rahma; Putranti, Respatiningtyas Amadea Danfi; Hanifa, Noha
MEDIA ILMU KESEHATAN Vol 13 No 1 (2024): Media Ilmu Kesehatan
Publisher : Universitas Jenderal Achmad Yani Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30989/mik.v13i1.1342

Background: The body injured potential for bleeding. Hemostatic drugs used to stop bleeding have many side effects. Kepok banana peels and bay leaves contain flavonoids and tannins which can stop bleeding.Objective: This study aims to determine the effect of a combination of kepok banana peel extract and bay leaves on bleeding time in Wistar rats. Methods: True experimental research type with post test only with control group design. 20 rats were divided into 5 groups, group K- was given aquadest, group K+ : epinephrine 1mg/1ml, group F1: kepok banana peel extract and bay leaves 25%:75%, group F2 was given extract 50:50%, and group F3 was given 75%:25% extract. Results: One Way ANOVA test obtained a value of p=0,002 showing that there was a significant difference in the time to stop bleeding for each treatment group, followed by the Post Hoc Games Howell test.Conclusion: The F3 group with a combination of 75% kepok banana peel extract and 25% bay leaf extract was the most effective group in accelerating bleeding time in Wistar rats

Uji Efektivitas Antibakteri Ekstrak Etanol Kulit Pisang Kepok (Musa Acuminata Balbisiana Colla) dan Daun Salam (Syzgiyum Polyanthum) Terhadap Bakteri Escherchia Coli Hanifa, Noha; Dhanti, Kurnia Ritma; Kurniawan, Kurniawan; Mulyanto, Arif
ORYZA ( JURNAL PENDIDIKAN BIOLOGI ) Vol 13 No 2 (2024): ORYZA: Jurnal Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi STKIP Bima

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33627/oz.v13i2.1960

This research aims to examine the effectiveness of the combination of bay leaf extract and kepok banana peel extract as an anti Escherichia coli bacteria. This type of research is experimental with a posttest only control group design with a comparison of kepok banana peel and bay leaves, F1 (25%: 75%), F2 (50%: 50%), F3 (75%: 25%). The research results showed that the most effective extract was F1 treatment (25%:75%) with an inhibitory power of 9.7mm2. The results of the statistical analysis showed that the inhibition test treatment against E. Coli bacteria using different concentrations showed a mean difference in the area of the inhibition zone with a significant value of 0.000 (p<0.05). The results of the Duncan Multiple Range Test (DMRT) showed that K+ (chloramphenicol) was significantly different from the concentrations of F1, F2 and F3. Testing the effectiveness of Kepok banana peel extract and bay leaf extract against E. coli bacteria showed that F1 treatment was effective in preventing bacterial growth against the F1 combination of 25% Kepok banana peel and 75% bay leaves.

PERBEDAAN KADAR TRIGLISERIDA PADA PEROKOK AKTIF DAN PASIF DI TERMINAL BULUPITU PURWOKERTO Athala, Syifani Aulia Ahnaf; Sulistiyowati, Retno; Sudarsono, Tantri Analisawati; Dhanti, Kurnia Ritma
Journal of Medical Laboratory and Science Vol 4 No 2 (2024): JMLS: Journal of Medical Laboratory and Science
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36086/medlabscience.v4i2.1922

Rokok mengandung berbagai macam zat kimia berbahaya salah satunya adalah nikotin. Zat tersebut dapat menstimulasi sistem simpatis adrenal yang menyebabkan peningkatan sekresi adrenalin di korteks adrenal dimana bagian ini merupakan tempat medulla adrenal berperan menghasilkan hormon adrenalin dan non-adrenalin saat stress yang dapat meningkatkan konsentrasi serum free fatty acid (FFA), lebih lanjut merangsang sintesis dan sekresi kolesterol di hati, seperti: mensekresi very low-density lipoprotein (VLDL) di hati dan dengan demikian meningkatkan kadar trigliserida darah. Tujuan penelitian ini untuk mengetahui perbedaan kadar trigliserida pada perokok aktif dan pasif di Terminal Bulupitu Purwokerto Kabupaten Banyumas. Jenis penelitian ini adalah observasional analitik menggunakan desain penelitian cross sectional dengan jumlah sampel sebanyak 24 orang responden yang diperoleh menggunakan metode purposive sampling. Pemeriksaan kadar trigliserida dalam darah menggunakan fotometer. Hasil penelitian ini menggunakan uji T independent didapatkan rata-rata kadar trigliserida pada perokok aktif sebesar 154,96 mg/dL, sedangkan pada perokok pasif didapatkan hasi sebesar 104,70 mg/dL. Kesimpulannya secara rerata terdapat perbedaan rerata antara kadar trigliserida pada perokok aktif dan pasif di Terminal Bulupitu Purwokerto Kabupaten Banyumas.

The effect of a combination of kepok banana peel (musa acuminata balbisiana colla) and bay leaf (syzygium polyanthum) extracts on bleeding time in wistar rats (rattus norvegicus) Sarinastiti, Deliza Dhiakhalda; Sudarsono, Tantri Analisawati; Dhanti, Kurnia Ritma; Wijayanti, Linda; Muniroh, Nur An-Nuha; Cahyaningrum, Winda Rahma; Putranti, Respatiningtyas Amadea Danfi; Hanifa, Noha
MEDIA ILMU KESEHATAN Vol 13 No 1 (2024): Media Ilmu Kesehatan
Publisher : Universitas Jenderal Achmad Yani Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30989/mik.v13i1.1342

Background: The body injured potential for bleeding. Hemostatic drugs used to stop bleeding have many side effects. Kepok banana peels and bay leaves contain flavonoids and tannins which can stop bleeding.Objective: This study aims to determine the effect of a combination of kepok banana peel extract and bay leaves on bleeding time in Wistar rats. Methods: True experimental research type with post test only with control group design. 20 rats were divided into 5 groups, group K- was given aquadest, group K+ : epinephrine 1mg/1ml, group F1: kepok banana peel extract and bay leaves 25%:75%, group F2 was given extract 50:50%, and group F3 was given 75%:25% extract. Results: One Way ANOVA test obtained a value of p=0,002 showing that there was a significant difference in the time to stop bleeding for each treatment group, followed by the Post Hoc Games Howell test.Conclusion: The F3 group with a combination of 75% kepok banana peel extract and 25% bay leaf extract was the most effective group in accelerating bleeding time in Wistar rats

Korelasi antara Kadar Karbon Monoksida di Udara dan Kadar COHb pada Pedagang Kaki Lima Sulistio, Ahmad Rizik; Dhanti, Kurnia Ritma; Sulistiowati, Retno; Juwita, Dina Ratna
JPP JURNAL KESEHATAN POLTEKKES PALEMBANG Vol 19 No 2 (2024): JPP (Jurnal Kesehatan Poltekkes Palembang)
Publisher : Poltekkes Kemenkes Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36086/jpp.v19i2.2490

Background: Air pollution is a foreign material or substance in the air that causes changes in the composition of the air from its normal state. Carbon monoxide (CO) is a colorless and odorless gas that can be dangerous if inhaled in large quantities. Sources of CO gas in the air include motor vehicles, as well as household items such as heaters that use kerosene and gas stoves. Carbon monoxide will replace oxygen in red blood cells, which can increase carboxyhemoglobin levels in the blood. Street vendors are people who sell goods and services in public spaces, especially on sidewalks or road shoulders, so they are at risk of being exposed to CO gas continuously. Method : This study uses an observational approach with a cross-sectional research design. The population in this study were street vendors on roads that are frequently passed by vehicles and rarely passed by vehicles as many as 30 samples, with a purposive sampling method. Results : CO levels in locations frequently passed by vehicles were 13 ppm while in places rarely passed by vehicles were 11 ppm and had an average COHb level of respondents in locations frequently passed by vehicles of 0.64% while in respondents in locations rarely passed by vehicles of 0.49%. Conclusion : There is a significant difference between COHb levels in street vendors on roads frequently and rarely passed by vehicles in the Purbalingga area, as evidenced by the Independent T Test result value of p = 0.010 (<0.05). There is a relationship between carbon monoxide levels and COHb levels in street vendors in the Purbalingga area, as evidenced by a p value of 0.001 (p <0.05). The Spearman correlation value of 0.555 indicates a strong relationship. Keywords : Air Pollution, carboxyhemoglobin (COHb), Carbon Monoxide, Street Vendors

Correlation Between Lead Levels and Percentage of Basophilic Stippling in the Blood of Silver Men Azzahra, Ratu; Dhanti, Kurnia Ritma; Djalil, Asmiyenti Djaliasrin; Sulistiyowati, Retno
Indonesian Journal of Global Health Research Vol 7 No 5 (2025): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v7i5.6818

The phenomenon of silver men has become increasingly prevalent in recent years. The direct application of metallic paint to the body and the nature of their activities are major factors contributing to potential lead expsure. Elevated blood lead levels can disrupt hematopoiesis, leading to various blood abnormalities, including the presence of basophilic stippling. Basophilic stippling is a distinctive marker indicative of heavy metal toxicity, particularly lead poisoning. This study aims to examine the correlation between blood lead levels and the presence of basophilic stippling in the blood of silver men in Purbalingga Regency and Purwokerto Sub-district. This study employed an observational analytical design with a cross-sectional approach. The research sample consisted of blood specimens from 14 silver men, selected using accidental sampling. Blood samples (3 mL) were collected from the respondents arms into EDTA tubes. Blood lead levels were analyzed using Atomic Absorption Spectrophotometry (AAS), while basophilic stippling was examined through Giemsa-stained blood smears observed under a microscope. The collected data were analyzed using Pearsons correlation test. The Pearson correlation test yielded a p-value of 0.045 (p 0.05) and a correlation coefficient of 0.542, indicating a strong positive correlation. There is a strong correlation between blood lead levels andthe presence of basophilic stippling in silver men. An increase in blood lead levels is accompanied by the occurrence of basophilic stippling, highlighting the potential toxic effects of lead exposure in this population.

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