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SIFAT ANTI RAYAP KATEKIN DARI GAMBIR (Uncaria gambir Roxb.) Nandika, Dodi
Prosiding University Research Colloquium Proceeding of The 8th University Research Colloquium 2018: Bidang Sosial Ekonomi dan Psikologi
Publisher : Konsorsium Lembaga Penelitian dan Pengabdian kepada Masyarakat Perguruan Tinggi Muhammadiyah 'Aisyiyah (PTMA) Koordinator Wilayah Jawa Tengah - DIY

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Abstract

Gambir is a dry extract from the leaves and branches of the Uncaria gambir Roxb plant. Its contains catechin that are quite large and have antioxidant properties. Catechin are flavonoid compounds contained in gambir (Uncaria gambir Roxb). Some previous studies have shown that catechins have antibacterial and antifungal properties. However, the antitermitics properties of these compounds have not been reported comprehensively. Preliminary research on the antitermitics properties of catechins was carried out by dissolving catechin in ethyl acetate (concentrations of 5%, 10%, 15%, and 20%) then applied to whatmann filter paper. Further research was carried out by impregnating catechin solutions (2.5%, 5% and 7.5% concentrations) into the rubber wood (Hevea brasiliensis) samples using the vacuum press method. The antitermitics properties was carried out based on the Japanese Industrial Standard (JIS) K-1571-2004 while testing the antitermitics properties of dry wood based on the Indonesian National Standard (SNI) 01-7207-2014. The results of the preliminary study showed that application of catechin had a significant effect on feedingpreferenceand mortality of termites C. curvignathusand C. cynocephalus.
Fingerprints, Antioxidant Activity, and Anti-Tyrosinase of Fungus Comb Extract Dewi, Trisni Untari; Diposani, Diva Putri; Rohaeti, Eti; Nandika, Dodi; Karlinasari, Lina; Arinana, Arinana; Santoso, Djoko; Witasari, Lucia Dhiantika; Rachmayanti, Yanti; Batubara, Irmanida
Jurnal Jamu Indonesia Vol. 8 No. 3 (2023): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v8i3.346

Abstract

Fungus comb is a structure made by termite colony Macrotermitinae with lots of potential because it contains secondary metabolites such as steroids, terpenoids, and saponins and compounds containing antioxidant and anti-tyrosinase properties. This study aimed to determine fungus comb extracts' fingerprint pattern, antioxidant capacity, and anti-tyrosinase activity. Thin layer chromatography (TLC) was used to determine fingerprint patterns. The resulting TLC pattern was processed with ImageJ and converted into a densitogram. The fungus comb was extracted with n-hexane and ethyl acetate. TLC results showed that fungus comb extracts contain steroids and terpenoids and have antioxidant properties. The antioxidant capacity assay was carried out with 2,2-diphenyl-1-picrylhydrazyl reagent. N-hexane extract had an antioxidant capacity value of 9.07 mg ascorbic acid equivalent (AAE)/g extract, while ethyl acetate extract had 6.04 mg AAE/g extract. Anti-tyrosinase activity assay was carried out with L-tyrosine and L-3,4-dihydroxyphenylalanine (L-DOPA) as substrates. N-hexane extract had a better anti-tyrosinase activity with IC50 of 1804 µg/mL for L-tyrosine and 6784 µg/mL for L-DOPA.