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Fingerprints, Antioxidant Activity, and Anti-Tyrosinase of Fungus Comb Extract Trisni Untari Dewi; Diva Putri Diposani; Eti Rohaeti; Dodi Nandika; Lina Karlinasari; Arinana Arinana; Djoko Santoso; Lucia Dhiantika Witasari; Yanti Rachmayanti; Irmanida Batubara
Jurnal Jamu Indonesia Vol. 8 No. 3 (2023): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v8i3.346

Abstract

Fungus comb is a structure made by termite colony Macrotermitinae with lots of potential because it contains secondary metabolites such as steroids, terpenoids, and saponins and compounds containing antioxidant and anti-tyrosinase properties. This study aimed to determine fungus comb extracts' fingerprint pattern, antioxidant capacity, and anti-tyrosinase activity. Thin layer chromatography (TLC) was used to determine fingerprint patterns. The resulting TLC pattern was processed with ImageJ and converted into a densitogram. The fungus comb was extracted with n-hexane and ethyl acetate. TLC results showed that fungus comb extracts contain steroids and terpenoids and have antioxidant properties. The antioxidant capacity assay was carried out with 2,2-diphenyl-1-picrylhydrazyl reagent. N-hexane extract had an antioxidant capacity value of 9.07 mg ascorbic acid equivalent (AAE)/g extract, while ethyl acetate extract had 6.04 mg AAE/g extract. Anti-tyrosinase activity assay was carried out with L-tyrosine and L-3,4-dihydroxyphenylalanine (L-DOPA) as substrates. N-hexane extract had a better anti-tyrosinase activity with IC50 of 1804 µg/mL for L-tyrosine and 6784 µg/mL for L-DOPA.
Feeding Preferences of Subterranean Termite Schedorhinotermes javanicus on Tusam (Pinus merkusii) Wood Kutana, Agung Nugrawan; Nandika, Dodi; Arinana, Arinana
Jurnal Sylva Lestari Vol. 13 No. 1 (2025): January
Publisher : Department of Forestry, Faculty of Agriculture, University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jsl.v13i1.1063

Abstract

Wood-destroying termites are quite influential in Indonesia, and the subterranean termite Schedorhinotermes javanicus is one of them. This study was conducted to determine the feeding preferences of subterranean termites S. javanicus on tusam (Pinus merkusii) wood and the influence of their environment. The bait woods made of tusam boards were installed at the experimental site. The weight loss and consumption of bait woods were studied with three test times (one, two, three) months of burial. The subterranean termite specimens attacking the bait woods, soil characteristics, and weather at the experimental field were also identified. The results demonstrated that the average air temperature at the experimental site was 25.4 ± 0.4°C, the average air humidity reached 88.8 ± 2.4%, and the light intensity at the experimental site ranged from 175.67 lux to 3889.7 lux. The results showed that S. javanicus quite actively attacked the bait wood. This can be seen from the high weight loss (35.03%) and the wood consumption (38.78 g). The weight loss after three exposure times in the experimental field was up to 13.54%, 16.38%, and 35.03%, respectively. The wood consumption during the same experimental period reached 14.9 g, 18.25 g, and 38.78 g, sequentially. The high weight loss and the consumption of bait wood indicate the high feeding preference of the subterranean termites S. javanicus on tusam wood. Keywords: field test, Pinus merkusii, Schedorhinotermes javanicus, weight loss, wood consumption