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All Journal Jurnal Sain Veteriner BERITA BIOLOGI Aquacultura Indonesiana Journal of Aquaculture and Fish Health Grouper: Jurnal Ilmiah Perikanan Journal of Marine and Coastal Science Berkala Penelitian Hayati Journal of Fisheries & Marine Jurnal Ilmiah Pertanian dan Peternakan
Hari Suprapto
Faculty Of Fisheries And Marine Universitas Airlangga
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary

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Efektifitas Ekstrak Tanaman Meniran (Phyllanthus niruri) Sebagai Antibakteri Edwardsiella tarda Secara In Vitro [Effectivity Of Meniran (Phyllanthus niruri) Extract as Edwardsiella tarda Antibacterial According In Vitro] Sudarno Sudarno; Fabi Aisah Setiorini; Hari Suprapto
Jurnal Ilmiah Perikanan dan Kelautan Vol. 3 No. 1 (2011): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v3i1.11631

Abstract

AbstractMedicinal plants proven to effectively cope with bacterial diseases one of which is the plant meniran (Phyllanthus niruri). Phyllanthus niruri can act as an antibacterial because it has an antibacterial ingredient content of flavonoids, alkaloids, phenols, and tannins. This shows that plants can be used as an antibacterial Phyllanthus niruri of E.tarda. The purpose of this study was to determine the ability of the antibacterial of Phyllanthus niruri extracts and to find out the best concentration of Phyllanthus niruri extracts as antibacterial E.tarda according in vitro. The experiment was conducted in January 2011, housed at the Laboratory of Bacteriology Juanda Fish Quarantine Center and Laboratory of Organic Chemistry and Technology Faculty of Airlangga University Saints. This research method is done by the Minimum Inhibitor Concentration (MIC) and Minimum Bacterial Concentration (MBC) with 12 treatment. The results showed that the best concentration for the treatment of the concentration of 0.0313 g / ml as indicated with no growth of bacteria and is the lowest concentration of the dilution. 
Kultur Sel Otak Ikan Kerapu Bebek (Cromileptes altivelis) dengan Menggunakan Media L-15, MEM Dan TCM [Cell Culture Brain Of Chromileptes altivelis With Medium L-15, MEM And TCM]. Hari Suprapto; Nunik Diantiwi
Jurnal Ilmiah Perikanan dan Kelautan Vol. 2 No. 2 (2010): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v2i2.11637

Abstract

Abstract Humpback grouper is high value commodity fish. The demand of domestic and foreign markets increases every year. The obstacles of Humpback grouper fish cultivation is a disease caused by Viral Nervous Necrosis (VNN). The virus attacks the brain leading to mass death. To prevent the disease, a research on VNN virus has to be started. The problem of the research is the lack of culture of fish tissue. Virus have specific location to tissue cell and species to be infected. Thereofore, first step must be done is growth from tissue cell which infection of virus. Cell culture is a technique to isolate cell, protoplasm, tissue or organ to be grown in a sterile condition with nutrition containing growth hormone to promote multiplication. Cell culture technique can be used in many basic applications in cell biology. The purpose of this research is to develop cell culture from Grouper fish brain for VNN research. The hope is culture can grow and develop well.Research executed at June until Desember 2009 in Laboratory In Vitro of Veterinari Faculty of Airlangga University and Laboratory Gastroenteritis Institute of Tropical Disease, Airlangga University, Surabaya. The result showed that brain cell culture of humpback grouper fish can be grow and multiply well in all three media, media L-15 cell growth was faster compared to media MEM and TCM.
Efektivitas Penggunaan Whole Cell Dari Vibrio alginolyticus Sebagai Vaksin Oral Melalui Artemia Pada Benih Ikan Kerapu Tikus (Chromileptes altivelis) [Effectiveness Of Using Vibrio alginolyticus Whole Cell As Oralvaccine With Artemia To Humpback Grouper Fry (Chromileptes altivelis) ] Hari Suprapto; Titik Desi Sukmawati
Jurnal Ilmiah Perikanan dan Kelautan Vol. 2 No. 2 (2010): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v2i2.11638

Abstract

Abstract Humpback grouper is the one of marine fish which can culture. The major problem of humpback grouper culture is high mortality at short time caused by pathogen infection include virus, bacterial, parasite and fungi. Pathogen infection at humpback grouper fry caused by bacterial from genus Vibrio spp. There was exceeding with vaccination. This research use oral vaccination with whole cell. Vibrio alginolyticus Whole cell as oral vaccine is suggested to prevent high mortality at humpback grouper fry. The purpose of this research is to know the successful about using Vibrio alginolyticus Whole Cell as Oral Vaccine to prevent high mortality of humpback grouper fry (Chromileptes altivelis). The experiment was done June until December 2009 at Gastroenteritis of Laboratory Tropical Disease Centre (TDC) of Airlangga University Surabaya and PT Benur Puteri Situbondo. The methods of this research is descriptive. Major parameter observed in this research was survival rate (%) and Relative Percentage Survival (RPS) of humpback grouper fry. Other parameter observed in this research was water quality include water temperature, pH, water salinity and Dissolved Oxygen (DO). Result of research shows that there are difference of survival rate between fingerling fish vaccination and fingerling fish non vaccination (control). Survival rate of humpback grouper fry which vaccine of Whole Cell 66 % even though survival rate of humpback grouper fry which non vaccine of Whole Cell (control) 46 %. Relative Percentage Survival (RPS) of humpback grouper fry which vaccine of Whole Cell 37 %. The value of RPS was resulted at this research shows low value so that must to perfecting and must to do again research. Water temperature 27-29oC, pH 7,3-7,5, water salinity 31-32 ppt and dissolved oxygen 5-6 ppm.
Pengaruh Pemberian Vaksin Whole Cell Killed Virus Terhadap Sintasan Udang Vannamei (Lithopenaeus vannamei) Yang Diinfeksi Whitespot Baculovirus (WSBV) [The Influence Of Whitespot Baculovirus (WSBV) Infection In Lithopenaeus vannamei By Giving Whole Cell Killed Virus Vaccine On Survival Rate.] Hari Suprapto; Angga Bahtera Siswanto; Boedi Setya Rahardja
Jurnal Ilmiah Perikanan dan Kelautan Vol. 2 No. 1 (2010): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v2i1.11670

Abstract

AbstractShrimp are susceptible to a wide variety of pathogens, one of each were viruses. Whitespot Baculovirus (WSBV) is one of virus attack in vannamei. Shrimp had been infected by WSBV will showed high mortality. One of Strategies for prophylaxis and control of WSBV is enhancement of disease resistant by using vaccines. Recently, quasi-immune response have been reported by which Penaeus japonicus surviving from WSBV infections possess a resistance against challenge WSBV (Venegas et al., 2000). This study was used Lithopenaeus vannamei that been vaccination with inactived-formaline WSBV virus cell or it called Whole Cell Killed Virus (WCKV) to know its responses to WSBV. The aim of this study was to know the effect Whole Cell Killed Virus vaccine to survival rate vannamei shrimp (Lithopenaeus vannamei) infected by Whitespot Baculovirus (WSBV). This study used descriptive study. The efficacy of vaccines made of inactivated WSSV with different dose. The dose are P1 (dose 0,01µg/ml each shrimp), P2 (dose 0,02 µg/ml each shrimp), P3 (dose 0,03 µg/ml each shrimp) dan P4 (non-vaccine (kontrol) injected by PBS). Primary parameter was survival rate (%). Secondary parameter was water quality which of temperature, pH, salinity and dissolved oxygen. The result showed that survival rate of L. Vannamei with different dose of WCKV vaccines was increases.
Influence of addition surimi wastewater to macronutrient content (nitrogen, phosphor, and potassium) of gracilaria sp. Liquid organic fertilizer Yudhistira, Ary; Suprapto, Hari; Sulmartiwi, Laksmi
Jurnal Ilmiah Pertanian dan Peternakan Vol. 1 No. 1 (2023): Juli
Publisher : Penerbit Goodwood

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35912/jipper.v1i1.2601

Abstract

Purpose: The purpose of this study was to determine the effect of adding surimi wastewater on the increase of nitrogen, phosphorus, and potassium content in Gracilaria sp. liquid organic fertilizer. Research Methodology: This research used an experimental method with a completely randomized design consisting of four treatments (0%, 80%, 90%, and 100% surimi wastewater) and five replications. Macronutrient content (nitrogen, phosphorus, and potassium) in the liquid fertilizer was measured after 14 days of fermentation to evaluate the effect of the treatments. Results: The results showed that the addition of surimi wastewater significantly increased the levels of nitrogen, phosphorus, and potassium in Gracilaria sp. liquid organic fertilizer (P < 0.05). The highest nutrient content was observed in treatments with higher concentrations of surimi wastewater, indicating a positive correlation between wastewater addition and nutrient enrichment. Conclusion: Surimi wastewater can be effectively utilized as an additive in Gracilaria sp. liquid organic fertilizer production to enhance its macronutrient composition. This approach demonstrates potential for sustainable fertilizer development using seafood processing by-products. Limitations: The study was limited by a relatively small sample size and a short observation period, which may restrict the generalizability of the findings. Longer-term studies and larger-scale trials are needed for broader validation. Contribution: This study provides insights into the sustainable utilization of surimi wastewater as a cost-effective nutrient source in liquid organic fertilizers, offering potential benefits for eco-friendly agricultural practices and waste management strategies.
Co-Authors . Suwarno A Kusyairi Abyan Farras Agustono - Ajik Azmijah Akhmad Syafroni Affandi Angga Bahtera Siswanto Boedi Setya Rahardja D. Handiyatno Dahlia Dahlia de Vries, Garry Cores Fabi Aisah Setiorini Faricha Risma Nurani Garry Cores de Vries Gunanti Mahasri I Made Satya Widnyana Istikhara Mentari Tito Kismiyati , Kusnoto Kusnoto Kusyairi Kusyairi Kusyairi, A Kusyairi, Kusyairi L. Sumartiwi Laksmi Sulmartiwi Madyowati, Sri Oetami Meidiana Salsabila Miftahul Jannah Mirni Lamid Muhajir Muhajir Muhammad Ghufron Muhammad Sungging Pradana N. Juni Triastuti Nadia Ayu Fadila Asshaufi Nadia Fierias Fathma Riza Nunik Diantiwi Nurul Qomariyah Oetami, Sri Oktantia Frenny Anggani Rahayu Kusdarwati Reny Lidhia Widi Samsisko Riris Ulfiana S. Prawesthirini Sari, Putri Desi Wulan Setiawati Sigit Sri Oetami Sri Oetami Madyowati Sudarno Sudarno Sudarno, Sudarno Sulmartiwi, L. Sumaryam Sumaryam Sumaryam Sumaryam Sumaryam, Sumaryam Titik Desi Sukmawati Tyfany Imanu Sabrina Widy Ayunanti Yenta Kusuma Wardhani Yudhistira, Ary Yulia Kartika