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All Journal Jurnal Sain Veteriner BERITA BIOLOGI Aquacultura Indonesiana Journal of Aquaculture and Fish Health Grouper: Jurnal Ilmiah Perikanan Journal of Marine and Coastal Science Berkala Penelitian Hayati Journal of Fisheries & Marine Jurnal Ilmiah Pertanian dan Peternakan
Hari Suprapto
Faculty Of Fisheries And Marine Universitas Airlangga
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary

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Potensi Bacillus licheniformis dan Streptomyces olivaceoviridis sebagai Penghambat Pertumbuhan Jamur Saprolegnia sp. Penyebab Saprolegniasis pada Ikan secara In Vitro [Potential of Bacillus licheniformis and Streptomyces olivaceoviridis as Inhibiting The Growth of Fungus Saprolegnia sp, Cause Saprolegniasis on Fish by Using In Vitro] Oktantia Frenny Anggani; Rahayu Kusdarwati; Hari Suprapto
Jurnal Ilmiah Perikanan dan Kelautan Vol. 7 No. 2 (2015): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v7i2.11196

Abstract

Abstract Saprolegniasis is a fungal disease on fish caused by Saprolegnia sp, which is saprophyte, damaging healthy tissue and makes the immune system in fish deacreased. The uniquennes of Saprolegnia sp has the main components of the cell wall in the form of chitin that was instrumental in shaping the structure of the tip growth of fungal hyphae. Control of fungal pathogenic Saprolegnia sp can use chitinolytic microorganisms based on ability to produce chitinase for example using bacteria. This study aims to potential of Bacillus licheniformis and Streptomyces olivaceoviridis as inhibiting the growth of fungus Saprolegnia sp, cause saprolegniasis on fish by using in vitro. The research method is experimental with completely randomized design (CRD), which consists of four treatments with five replications. The experimental used is A (Saprolegnia sp and Bacillus licheniformis), B (Saprolegnia sp and Streptomyces olivaceoviridis), C negative control (Saprolegnia sp) and D positive control (Saprolegnia sp and Ketokonazol 2 %). The main parameters measured were observed inhibition zone on each treatment. Supporting parameters were observed is an observation of abnormal hpyphae structure after being induced by bacteria Bacillus licheniformis dan Streptomyces olivaceoviridis. Data were analyzed using analysis of variants (ANOVA) and to know the difference between treatments were determined by Tukey honestly significant difference (Tukey HSD) Test. The results showed that the potential of chitinolytic Bacillus licheniformis can provide a good barrier of 4,62 cm by 5,48 cm compared Streptomyces olivaceoviridis in inhibiting the growth Saprolegnia sp. Suggestions in this research is the need to further research on the value of chitinase Bacillus licheniformis and Streptomyces olivaceoviridis. Further research by using in vivo.
Deteksi Koi Herpesvirus (KHV) pada Ikan Nila (Oreochromis niloticus) yang Diinfeksi secara Buatan [ Detection of Koi Herpesvirus (KHV) in Nile Tilapia (Oreochromis niloticus) Infected by Artificially Infection ] Hari Suprapto; Suwarno Suwarno; Muhammad Sungging Pradana
Jurnal Ilmiah Perikanan dan Kelautan Vol. 7 No. 1 (2015): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v7i1.11230

Abstract

Abstract Koi Herpesvirus (KHV) was formerly known as Cyprinid herpesvirus-3 (CyHV-3) and carp nephritis interstitial and necrosis gill virus (CNGV) is a virus that infects common carp and koi (Cyprinus carpio and C. carpio koi) in farmed and wild population. KHV cause disease at a temperature of 18-25 °C with mortality rate of 80-90 % in fry and adult fish. Currently KHV also detected in tilapia from the results of monitoring in the field. The presence of KHV in tilapia can occur as a result of maintenance in cages adjacent to the infected carp. KHV diagnostic method currently based on case definition and PCR (Polymerase Chain Reaction) detection. The basic concept of PCR is one DNA molecule is used to produce two copies, then four, then eight and so forth through multiplication by polymerase. PCR results sometimes indicated the presence of a faint band caused a low amount of virus, so it is necessary to investigate the presence of KHV DNA in tilapia using different doses of infection. This study aimed to determine the KHV infectivity in nile tilapia were infected by artificially infection and determine dose KHV infection that can infect nile tilapia. The study design used true experimental with with the presentation of descriptive data. Dose of viral infection are 1 ID50, 10 ID50, 100 ID50 and 1000 ID50. The results showed that no clinical symptoms of KHV infected in nile tilapia. The results of electrophoresis of PCR products showed that the mucus of nile tilapia were infected with a 1000 ID50 immersion dose showed thin bands. The same results are also shown in the gill of nile tilapia infected by gill spray at 1000 ID50 dose. Fish were infected by injection, KHV was not detected in mucus, gill, kidney and liver. The results above show nile tilapia cannot be infected by KHV on various treatment
Identifikasi Koi Herpesvirus pada Dosis yang Berbeda dengan Metode Imunohistokimia Streptavidin Biotin pada Ikan Nila (Oreochromis niloticus) [Identification of Koi Herpes Virus At Different Dose with Streptavidin Biotin Methods Immunohistochemistry on Tilapia (Oreochromis niloticus)] Hari Suprapto; Faricha Risma Nurani; Suwarno Suwarno
Jurnal Ilmiah Perikanan dan Kelautan Vol. 6 No. 2 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v6i2.11277

Abstract

Abstract KHV is a viral disease in goldfish and Koi Fish (Cyprinus carpio) is a highly contagious, infecting all ages or sizes of fish and aquaculture systems. This disease results in mortality between 80% - 100% of the total fish population, with an incubation period of between 1-7 days. KHV infection triggered by a drop in ambient temperature so it is referred to as a virus that attacks when cold (a cold virus). The disease attacks the temperature range of 18-28 ° C and can cause death. This virus attacks can occur at any age of the fish ranging in size from the seed to the parent. The most prominent clinical symptoms due to KHV infection is the sudden death 1-2 days after infection. Other clinical symptoms are necrosis of the gills, sunken eyes, bleeding at the gills, hemorraghe, excess mucus production in the body, and secondary bacterial infections or parasitic infestations. Streptavidin biotin immunohistochemical method was applied to the study of cells and tissues by staining imunostaining. Technique of determining the existence of (location) antigen (target protein) in tissue using antigen-antibody reaction that begins with histotenik procedure is the procedure of making tissue sections (histology). This method has high sensitivity and fast so that it can be applied to the detection of KHV antigen in tissues of fish Tilapia (Orechromis niloticus). This study aims to determine the presence of KHV antigen in tilapia gills after infection. The method used in this study is the experimental method. Dose of virus titer used was 1 ID50, ID50 10, 100 ID50, ID50 1000. The results showed that the Streptavidin Biotin immunohistochemical examination imunopatologi able to detect KHV virus Ag in the gill tissue of tilapia (Oreochromis niloticus) were infected with different doses. In all treatments showed on all the gills are KHV antigen is indicated by the presence of a golden brown color on the gill epithelium. Advised Streptavidin biotin immunohistochemistry test was applied to the detection of the presence or absence of fish-carp KHV carrier as a routine control program and control, including the prevention and outbreak of KHV in Indonesia, because of KHV were attacked in tilapia was persistent and did not show clinical symptoms
Pengaruh Pemberian Sari Kecambah Kacang Hijau terhadap Kadar Malondialdehid pada Ikan Lele Dumbo (Clarias gariepinus) yang Terinfeksi Bakteri Aeromonas hydrophila [Effect of Mung Bean Sprouts Essences against Malondialdehyde Levels In African catfish (Clarias gariepinus) Infected by Bacteria Aeromonas hydrophila ] Hari Suprapto; Widy Ayunanti; Boedi Setya Rahardja
Jurnal Ilmiah Perikanan dan Kelautan Vol. 6 No. 2 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v6i2.11302

Abstract

Abstract African catfish (Clarias gariepinus) is a type of freshwater fish consumption with elongated body and smooth skin. Bacterial disease that often strikes African catfish and can cause death as well as a cause of primary infection is A.hydrophila. Bacterial infection may be one cause of the synthesis of lipid peroxidation which is an indication of the emergence of free radicals. And the end result of lipid peroxidation is malondialdehyde. Sprouts are the new little plants grow from seeds planted beans. Green bean seed germination will enrich vitamins including vitamin E, green beans or α-tocopherol. Vitamin E in maintaining the stability of the plasma membrane and protects the cell structure against damage caused by free radicals is by blocking the reaction initiation and propagation of lipid peroxidation reactions interrupt. Data analyze of this research was used Varian Analyze (ANAVA) and to found the lowest level was used Duncan Test. The average yield of malondialdehyde levels in the negative control treatment is 0.0386 ng / 100 mg mass, positive control (1 ml Aeromonas hydrophila 105 cfu per 5 liter) is 0.0962 ng / 100 mg mass, sprout extract treatment (0 , 1 ml per fish) is 0.05 ng / 100 mg mass, germination and pollen treatment Aeromonas hydrophila is 0.0626 ng / 100 mg mass. From these results indicate that the highest levels found in the positive control treatment while the lowest levels found in the negative control. These results also suggest that decreased levels of malondialdehyde using pollen germination is very significantly different with P < 0,01.
Predileksi dan Perubahan Patologi Anatomi pada Ikan Maskoki (Carassius auratus) akibat Infestasi Lernaea cyprinacea Di Kabupaten Tulungagung [Predilection and Anatomical Pathology Changes in Goldfish (Carassius auratus) Due to Lernaea Cyprinacea Infestation in Tulungagung] Kismiyati Kismiyati; Nadia Ayu Fadila Asshaufi; Hari Suprapto
Jurnal Ilmiah Perikanan dan Kelautan Vol. 6 No. 2 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v6i2.11303

Abstract

Abstract Goldfish (Carassius auratus) is one of the most popular freshwater fish, therefore they has a very great potential market. Increased effort in Goldfish (Carassius auratus) aquaculture industry, fish farmer also faced greater problems. One of it is disease caused by ectoparacites Lernaea cyprinacea. The predilection of Lernaea cyprinacea can be be directly visible, by the presence of parasites attached to the body surface, fins, gills, eyes and mouth of fish. Lernaea cyprinacea attack on fish can cause pathological change such as lesions and nodules on the skin and fins. This research was conducted in March-November 2013. Location of sampling conducted in Tulungagung East Java while Goldfish (Carassius auratus) pathological examination in the Laboratory of Faculty of Fisheries and Marine Airlangga University. The purpose of this study was to determine the predilection of Lernaea cyprinacea that mostly infested on Goldfish (Carassius auratus) and knowing the change of anatomical pathology in Goldfish (Carassius auratus) infested by Lernaea cyprinacea. The method used was a survey method with random sampling technique and change in anatomic pathology described by the scoring method. The result showed that the predilection of Lernaea cyprinacea mostly infested on Goldfish (Carassius auratus) on dorsal and caudal fin, anatomic pathology changes on Goldfish (Carassius auratus) that infested by Lernaea cyprinacea are abscess, haemorhage, laceration, and fins damaged
Pengaruh Ekstrak Rimpang Kencur (Kaempferia galanga L.) terhadap Tingkat Kesembuhan Benih Ikan Lele Dumbo (Clarias sp.) yang Terinfeksi Saprolegnia sp. [Effect of Rhizome Extract (Kaempferia kalanga L.) for the Cure Rate of Catfish (Clarias sp.) Infected by Saprolegnia sp. ] Hari Suprapto; Nadia Fierias Fathma Riza; Rahayu Kusdarwati
Jurnal Ilmiah Perikanan dan Kelautan Vol. 6 No. 2 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v6i2.11304

Abstract

Abstract Saprolegnia sp. is a fungus that causes the disease Saprolegniasis on eggs and fresh water fish such as catfish (Clarias sp.). Treatment of Saprolegniasis have done using chemical drugs such as malachite green, formalin, sodium chloride and asetic acid. However, the use of chemical drugs is bad for the environment and biota. The purpose of this study is to determined the antifungal activity from rhizome extract (Kaempferia galanga L.) for the cure rate of catfish fry (Clarias sp) infected by Saprolegnia sp. The research was conducted on June 2013 at Fisheries and Marine Faculty of Airlangga University. The method used in this study is experimental method using Kruskall Wallis followed by Two Sample Independent test design with 5 treatments and four repetitions. Results showed that the extract of Kaempferia galanga L. has antifungal activity to recovery infected catfish fry. The optimal concentration from this research is 64%. The existence of antifungal activity of Kaempferia galanga L. extracts to Saprolegnia sp. showed that Kaempferia galanga L. extract consists some compounds as fungistatic and fungicidal such as flavonoid, saponin, phenolic and essential oil compounds.
Uji Aktivitas Antifungi Perasan Daun Kemangi (Ocimum sanctum Linn.) terhadap Aspergilllus terreus secara In Vitro [Antifungal Activity Test of Basil Leaves Juice (Ocimum sanctum Linn.) against Aspergilllus terreus by in Vitro] Sudarno Sudarno; Tyfany Imanu Sabrina; Hari Suprapto
Jurnal Ilmiah Perikanan dan Kelautan Vol. 6 No. 2 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v6i2.11305

Abstract

Abstract Aspergillus terreus is a fungus that causes aspergillosis disease. The infected fishes showed grey white patches over the body. Haemorrhagic ulceratic patches were observed on the gill and skin. The infections resulted in the death of the fishes. The use of chemicals to control fungal attack A. terreus can harm fish, the environment and humans who eat them. Treatment of fungal diseases that use a lot of chemicals that can harm the fish, the environment and humans who eat them. The use of medicinal plants is a safe way to inhibit and kill fungus growth as well as environmentally friendly. One of them uses the basil plant (Ocimum sanctum). The purpose of this study was to determine the minimum concentration of activity and basil leaf juice (O. sanctum Linn) as antifungal against A. terreus growth in vitro . The experiment was conducted at the Laboratory of the Faculty of Fisheries and Marine Airlangga University in July 2013. The method used in this research that the paper disc diffusion method and diffusion pitting. The analysis used in this study is descriptive statistica. The results of research using the juice of basil leaves (O. santum) of juice concentration of 50% (2,5 ml juice of basil leaves + 2,5 ml NaCl) to concentration 100% (5 ml juice of basil leaves) did not produce a clear zone around the paper discs and pitting, it is the same as the negative control. The positive control did not show fungus growing on Sabouraud Dextrose Agar medium (SDA).
Tingkat Kejadian Aeromonasis pada Ikan Koi (Cyprinus carpio carpio) yang Terinfeksi Myxobolus koi pada Derajat Infeksi yang Berbeda [ Aeromonasis Incidence Rate In Koi (Cyprinus carpio carpio) That Infected Myxobolus koi At Different Degrees Of Infection ] Gunanti Mahasri; Riris Ulfiana; Hari Suprapto
Jurnal Ilmiah Perikanan dan Kelautan Vol. 4 No. 2 (2012): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v4i2.11568

Abstract

Abstract Myxobolus infection in koi fish allows followed by opportunistic microorganisms infection. Aeromonas hydrophila bacteria classified as opportunistic because it can cause illness in certain condition such as when environmental conditions change, stress and the condition of the host that has been infected by the parasite. This study purpose to determine the incidence rate Aeromonasis in koi (Cyprinus carpio carpio) infected Myxobolus koi at different degrees of infection. The results showed that of 120 samples taken, infected with M. koi in a mild degree of infection is 68.3%. Furthermore the medium infection is 22.5%, 1.7% severe infection and uninfected fish 7.5%. Each level of infection of M. koi taken samples for examination bacteria with skin samples and water. The results of bacterial examination showed that 17 positive samples of fish infected with A. hydrophila (85%) from 20 fish sample. 17 positive samples of fish infected with A. hydrophila consist of 3 sample normal fish, 7 sample M. koi mild infection, 6 sample M. koi medium infection and 1 sample severe infection. The results showed that the fish infected or not infected with M. koi, positive with A. hydrophila infection that needs to be done by using a bacterial colony calculation method of determining the Angka Lempeng Total (ALT) or Total Plate Count (TPC). The calculation results from the number of bacterial colony in various levels of infection of M. koi are sample 1 (normal koi fish) of 1.15 x108 CFU/ml, sample 2 (koi fish with a mild infection of M. koi) as much as 1.3 x108 CFU/ml. While the sample 3 (koi fish with a medium infection of M. koi) 2.02 x108 CFU/ml and sample 4 (with the severe infection of M. koi) 2.60 x108 CFU/ml.
Strategi Bakteri Probiotik untuk Menekan Pertumbuhan Bakteri Patogen di dalam Pencernaan Kerapu Chromileptes altivelis dengan Memproduksi Beberapa Bakterial Substansi [ Probiotic Bacteria Strategy For Depressing Patogenic Bacteria Growth In Digestion Of Grouper Fish (Chromileptes altivelis) With Producing Of Substancy Bacterial ] Agustono Agustono; Hari Suprapto; Muhajir Muhajir
Jurnal Ilmiah Perikanan dan Kelautan Vol. 4 No. 2 (2012): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v4i2.11572

Abstract

Abstract The aim of the research is looking for probiotic bacteria strategy for depresing the growth of patogenic bacteria in digestion of grouper fish. Some of anti bacteria can be produced by bacteria, such as protease, bacteriocia (germicidine, pyrocydia, circulin), amilase lipase, and lyzozym. In this case, Bacillus sp. is chosen because it can produce antibacteria againts Vibrio sp. and probiotic multifactor effect, that is enzyme production and competition of nutrient and space.
Pemantauan Virus dengan Metode PCR (Polymerase Chain Reaction) di Pantai Utara Jawa Timur [Monitoring Virus By PCR Method (Polymerase Chain Reaction) In North Coast, East Java] Hari Suprapto; Yulia Kartika
Jurnal Ilmiah Perikanan dan Kelautan Vol. 4 No. 1 (2012): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v4i1.11587

Abstract

Abstract The disease most dangerous for the cultivation activity is virus. Viruses are organisms subseluler that contain only nucleic acid (RNA or DNA) as genetic material. Koi Herpes Virus is one type of virus that causes mortality in cultured Cyprinids. KHV disease in Indonesia started in Blitar, East Java on March 2002 because the entry of imported koi fish that carry the virus KHV, while mortality prosentase could reach 80% - 85%, which causes loss of about 5 billion rupiah. In addition of KHV, there are several types of viral diseases in shrimp is White Spot Syndrome Virus (WSSV), Taura Syndrome Virus (TSV), dan Yellow Head Virus (YHV). Disease can cause losses in farming activities, such as WSSV. WSSV is an endemic disease since 1995. disease WSSV is exotic viral disease that attacks the shrimp monodon in 1998/1999 has resulted in decreased production of very large, so the Indonesian shrimp exports down 33,000 tons. Treatment of viral diseases is difficult because the virus resistant to certain antibiotics and chemical compounds. Therefore, prevention needs to be done, one through the monitoring activities conducted on the northern coast of East Java. The method implemented is monitoring in location and identification of viruses by PCR (Polymerase Chain Reaction). Monitoring in location includes water quality measurements and sampling. Identification of virus carried by IQ 2000TM. The identification procedure includes extraction, amplification and electrophoresis. Regional monitoring conducted on the northern coast of East Java includes Gresik, Lamongan, Tuban, Bangkalan, Sampang, Pamekasan, and Sumenep. Water quality at locations quite well. Results activities of monitoring on the northern coast of East Java is disease White Spot Syndrome Virus (WSSV) was found positive in several locations: Gresik, Lamongan and Tuban, while the virus Taura Syndrome Virus (TSV) and Yellow Head Virus (YHV) was not found at all locations . In tilapia, disease Koi Herpes Virus (KHV) was found positive in Tuban.
Co-Authors . Suwarno A Kusyairi Abyan Farras Agustono - Ajik Azmijah Akhmad Syafroni Affandi Angga Bahtera Siswanto Boedi Setya Rahardja D. Handiyatno Dahlia Dahlia de Vries, Garry Cores Fabi Aisah Setiorini Faricha Risma Nurani Garry Cores de Vries Gunanti Mahasri I Made Satya Widnyana Istikhara Mentari Tito Kismiyati , Kusnoto Kusnoto Kusyairi Kusyairi Kusyairi, A Kusyairi, Kusyairi L. Sumartiwi Laksmi Sulmartiwi Madyowati, Sri Oetami Meidiana Salsabila Miftahul Jannah Mirni Lamid Muhajir Muhajir Muhammad Ghufron Muhammad Sungging Pradana N. Juni Triastuti Nadia Ayu Fadila Asshaufi Nadia Fierias Fathma Riza Nunik Diantiwi Nurul Qomariyah Oetami, Sri Oktantia Frenny Anggani Rahayu Kusdarwati Reny Lidhia Widi Samsisko Riris Ulfiana S. Prawesthirini Sari, Putri Desi Wulan Setiawati Sigit Sri Oetami Sri Oetami Madyowati Sudarno Sudarno Sudarno, Sudarno Sulmartiwi, L. Sumaryam Sumaryam Sumaryam Sumaryam Sumaryam, Sumaryam Titik Desi Sukmawati Tyfany Imanu Sabrina Widy Ayunanti Yenta Kusuma Wardhani Yudhistira, Ary Yulia Kartika