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Biodegradation activities of microplastic polymers by Agelas conifera spongesymbiotic bacteria Ismet, Meutia Samira; Srimariana, Endang Sunarwati; Cahyati, Nur Alam Dwi; Anggraini, Nurlita Putri; Simbolon, Lenni Mariana; Cakasana, Nadya; Sari, Anita Edya Melliana; Rafi, Mohamad
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan Vol 14, No 2 (2025): JUNE 2025
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.14.2.35939

Research on microplastic (MP) accumulation in marine organisms' tissue have been widely conducted, as well as research on microplastic biodegradation by microbial organisms from the environment. Agelas conifera sponge is proven to accumulate MP in its tissue as well as having bacteria as symbiotic organism. This research aims to screen and study biodegradation activities of A. conifera symbiotic bacteria on MP polymers, also to identify the species of those bacteria isolates. Bacterial Isolation of A. conifera sponge tissue was using Zobell 2216E agar media. Screening and biodegradation assay of the bacteria isolates was conducted on Marine Salt Media agar that was added with 0,5 g of MP polymers, using Kroc-Boral method. Diameter of clear (bacterial growth) zone surrounding Kroc-Boral indicated the biodegradation activity of the bacterial isolates. Statistical analysis on the activity was using Two Way ANOVA. Results showed that there were 9 bacterial isolates of 53 that could degrade several MP polymers, with different activities (ANOVA, p value 0.05). High and low-density polymers (Linear- Low Density PolyEthylene/ LLDPE, Polyethylene/PE, and Polyvinyl Chloride/ PVC) were found to be degraded by all 9 isolates. Meanwhile, Polyethylen Taraphtalene (PET) and Polystyrene (PS) only degraded by 8 and 3 isolates, respectively. There are only 5 isolates that have high biodegradation index (1.5) on different polymers, which mostly on PET polymer. Isolate Ac8MP found to be the most bacteria that have degradation index on all polymers more than 1. This research proves that symbiotic bacteria could have a biodegradation mechanism on MP polymers that accumulates by sponge-host. However, further study needs to be done to understand the mechanisms and effectiveness of sponge-bacterial biodegradation activity.Keywords:Dumbo catfishLeukocytesErythrocytesGlucoseMusa AcuminataAeromonas hydrophila

Presenting identification keys and future study on seagrass Halophila major in Indonesia Darus, Robba Fahrisy; Bengen, Dietriech Geoffrey; Zamani, Neviaty Putri; Ismet, Meutia Samira
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan Vol 14, No 2 (2025): JUNE 2025
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.14.2.44166

Halophila major is a new species with a wide distribution across various regions in Indonesia. The species was identified in 2020 through a combination of morphology and molecular approaches. Several studies have shown that it has a similar morphology to H. ovalis, which causes significant confusion during identification. Therefore, this study aims to describe the key identification and habitat of H. major and summarize seagrasses study opportunities based on the trend of published articles. A bibliometric analysis was used to summarize the habitat, morphometrics, molecular confirmation, and trend of seagrass topics in Indonesia. Based on the scientific articles, H. major has bigger morphometrics than H. ovalis. In addition, the number of paired and branching cross veins was reported to be an identification key of H. major. The species was also considered a deep ovalis found in 2-4 m depth. Genetically, the Internal Transcribed Spacer (ITS) gene marker was appropriate to showits phylogenetic tree. H. major was also classified into different clades with H. ovalis due to various factors. The results showed that genetics, tourism, and restoration were topics with the potential to be explored in the future. This study recommended collaborating in multi-institution to transfer knowledge, technologies, and project arrangements for seagrass exploration.Keywords:BibliometricHalophilaMarine angiospermaeMarin plantRediscoverySpecies

Biodegradation activities of microplastic polymers by Agelas conifera spongesymbiotic bacteria Ismet, Meutia Samira; Srimariana, Endang Sunarwati; Cahyati, Nur Alam Dwi; Anggraini, Nurlita Putri; Simbolon, Lenni Mariana; Cakasana, Nadya; Sari, Anita Edya Melliana; Rafi, Mohamad
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan Vol 14, No 2 (2025): JUNE 2025
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.14.2.35939

Research on microplastic (MP) accumulation in marine organisms' tissue have been widely conducted, as well as research on microplastic biodegradation by microbial organisms from the environment. Agelas conifera sponge is proven to accumulate MP in its tissue as well as having bacteria as symbiotic organism. This research aims to screen and study biodegradation activities of A. conifera symbiotic bacteria on MP polymers, also to identify the species of those bacteria isolates. Bacterial Isolation of A. conifera sponge tissue was using Zobell 2216E agar media. Screening and biodegradation assay of the bacteria isolates was conducted on Marine Salt Media agar that was added with 0,5 g of MP polymers, using Kroc-Boral method. Diameter of clear (bacterial growth) zone surrounding Kroc-Boral indicated the biodegradation activity of the bacterial isolates. Statistical analysis on the activity was using Two Way ANOVA. Results showed that there were 9 bacterial isolates of 53 that could degrade several MP polymers, with different activities (ANOVA, p value 0.05). High and low-density polymers (Linear- Low Density PolyEthylene/ LLDPE, Polyethylene/PE, and Polyvinyl Chloride/ PVC) were found to be degraded by all 9 isolates. Meanwhile, Polyethylen Taraphtalene (PET) and Polystyrene (PS) only degraded by 8 and 3 isolates, respectively. There are only 5 isolates that have high biodegradation index (1.5) on different polymers, which mostly on PET polymer. Isolate Ac8MP found to be the most bacteria that have degradation index on all polymers more than 1. This research proves that symbiotic bacteria could have a biodegradation mechanism on MP polymers that accumulates by sponge-host. However, further study needs to be done to understand the mechanisms and effectiveness of sponge-bacterial biodegradation activity.Keywords:Dumbo catfishLeukocytesErythrocytesGlucoseMusa AcuminataAeromonas hydrophila

Presenting identification keys and future study on seagrass Halophila major in Indonesia Darus, Robba Fahrisy; Bengen, Dietriech Geoffrey; Zamani, Neviaty Putri; Ismet, Meutia Samira
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan Vol 14, No 2 (2025): JUNE 2025
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.14.2.44166

Halophila major is a new species with a wide distribution across various regions in Indonesia. The species was identified in 2020 through a combination of morphology and molecular approaches. Several studies have shown that it has a similar morphology to H. ovalis, which causes significant confusion during identification. Therefore, this study aims to describe the key identification and habitat of H. major and summarize seagrasses study opportunities based on the trend of published articles. A bibliometric analysis was used to summarize the habitat, morphometrics, molecular confirmation, and trend of seagrass topics in Indonesia. Based on the scientific articles, H. major has bigger morphometrics than H. ovalis. In addition, the number of paired and branching cross veins was reported to be an identification key of H. major. The species was also considered a deep ovalis found in 2-4 m depth. Genetically, the Internal Transcribed Spacer (ITS) gene marker was appropriate to showits phylogenetic tree. H. major was also classified into different clades with H. ovalis due to various factors. The results showed that genetics, tourism, and restoration were topics with the potential to be explored in the future. This study recommended collaborating in multi-institution to transfer knowledge, technologies, and project arrangements for seagrass exploration.Keywords:BibliometricHalophilaMarine angiospermaeMarin plantRediscoverySpecies

EVALUASI MULTITEMPORAL INDEKS KESEHATAN KARANG DI KAWASAN KONSERVASI PULAU PIEH DAN LAUT SEKITARNYA (2021-2024): ANALISIS BIOFISIK DAN IMPLIKASINYA TERHADAP PENGELOLAAN BERBASIS EKOSISTEM Noferya, Efi; Bengen, Dietriech Geoffrey; Zamani, Neviaty Putri; Ismet, Meutia Samira; Muslim, Ryan Adhitia; Arisandi, Defrian Marza; Rahmat, Fadhlan Basiluddin; Ilham, Yuwanda; Utama, Agung Putra; Yennafri, Yennafri; Samsuardi, Samsuardi; Miswandi, Muhammad
Jurnal Kebijakan Perikanan Indonesia Vol 17, No 1 (2025): (Mei) 2025
Publisher : Badan Penyuluhan dan Pengembangan Sumberdaya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jkpi.17.1.2025.59-75

Terumbu karang merupakan ekosistem laut yang memiliki peran penting dalam menjaga biodiversitas dan ketahanan lingkungan pesisir. Namun, dalam beberapa dekade terakhir, degradasi terumbu karang terus meningkat akibat aktivitas antropogenik dan perubahan iklim. Penelitian ini bertujuan untuk menganalisis Indeks Kesehatan Karang (Coral Health Index / CHI) di Kawasan Konservasi Pulau Pieh dan perairan sekitarnya dalam periode 2021-2024 menggunakan pendekatan biofisik dan statistik. Analisis dilakukan terhadap 16 stasiun pengamatan, menggunakan data parameter bentik (tutupan karang hidup, resiliensi) dan biomassa ikan karang yang diperoleh melalui metode Underwater Photo Transect (UPT) dan Underwater Visual Census (UVC). Hasil penelitian menunjukkan bahwa nilai CHI berkisar antara 3 hingga 10, dengan beberapa stasiun menunjukkan tren peningkatan sementara lainnya mengalami stagnasi atau penurunan. Analisis Spearman correlation mengindikasikan bahwa parameter kualitas air (pH, DO, suhu, dan salinitas) tidak memiliki hubungan signifikan terhadap CHI pada semua tahun pengamatan (p-value > 0,05). Analisis Principal Component Analysis (PCA) menunjukkan bahwa faktor kualitas air bukan penentu utama perubahan CHI, tetapi tekanan ekologis dan aktivitas antropogenik lebih berperan dalam menentukan kondisi kesehatan terumbu karang. Hasil penelitian ini memberikan rekomendasi untuk pengelolaan berbasis ekosistem guna menjaga keberlanjutan ekosistem terumbu karang.Coral reefs are marine ecosystems that play a crucial role in maintaining biodiversity and coastal environmental resilience. However, in recent decades, coral reef degradation has continued to increase due to anthropogenic activities and climate change. This study aims to analyze the Coral Health Index (CHI) in the Pieh Island Conservation Area and its surrounding waters from 2021 to 2024 using biophysical and statistical approaches. The analysis was conducted at 16 observation stations using benthic parameters (live coral cover, resilience) and reef fish biomass data collected through the Underwater Photo Transect (UPT) and Underwater Visual Census (UVC) methods. The results indicate that CHI values range from 3 to 10, with some stations showing an increasing trend while others experience stagnation or decline. Spearman correlation analysis indicates that water quality parameters (pH, DO, temperature, and salinity) do not have a significant relationship with CHI in all observation years (p-value > 0.05). Principal Component Analysis (PCA) shows that water quality factors are not the main determinants of CHI changes; instead, ecological pressure and anthropogenic activities play a more significant role in determining coral reef health. This study provides recommendations for ecosystem-based management to ensure the sustainability of coral reef ecosystems.

Effective DNA extraction method for metagenomic analysis of rhizosphere bacteria from mangrove sediments Maysaroh, Siti; Ismet, Meutia Samira; Subhan, Beginer; Andini, Rita; Sembiring, Enny Rimita; Anggraini, Nurlita Putri
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan Vol 12, No 2 (2023): AUGUST 2023
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.12.2.32850

Mangrove, Rhizophora mucronata, grows in the intertidal area, which contains much organic matter and varying salinity. The organic matter content is influenced by the bacterial community that inhabits the ecosystem, but information regarding the bacterial community, especially in the mangrove root system, is not widely available. There are several challenges in completing this information, one of which is that the method used is still in a conventional form. Developments in environmental DNA analysis can support and complement this information. However, this method must be optimized because the organic matter content and salt variations affect the extraction results. Thus, this study aimed to determine the optimal approach for extracting bacterial DNA from mangrove sediments. The analysis used two methodologies: manual DNA extraction techniques based on buffer modification and DNA extraction kits. There were four different treatments, namely the soil DNA isolation plus kit (M1), the fecal / soil microbial quick-DNA miniprep kit (M2), glass powder with charcoal (M3), and glass powder with skimmed milk (M4). DNA samples were obtained from each method and assessed for concentration and purity using a nanodrop. In addition, the resulting DNA's quality was analyzed using 1.5% agarose. The results obtained were in the M2 treatment, which showed optimal results compared to the others. M2 uses a bead-based beating and spin column method to achieve optimal DNA concentration through high molecular weight. The DNA obtained was also protein-free, and several samples were contaminated with humic acid, namely KL.S1, KL.S4, and T7.S4.Keywords:Bacteria 16SBead beatingDNA ExtractionSedimentSpin column

Phytoplankton in Eutrophic Waters of Hurun Bay: An Examination of Composition Patterns, Structure, and Impacts on Aquatic Ecosystems Munru, Maestro; Ismet, Meutia Samira; N.Natih, Nyoman Metta; Subhan, Beginer; Aziz, Nasrul
International Journal of Marine Engineering Innovation and Research Vol 9, No 3 (2024)
Publisher : Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j25481479.v9i3.21625

Ecological conditions are shifting due to increased anthropogenic activities along coastal areas. These activities are affecting the life cycle of aquatic organisms, especially phytoplankton. This study, conducted in the unique ecosystem of Hurun Bay Lampung, aims to analyze the composition and structure of phytoplankton communities in eutrophic waters and water quality conditions. Sampling was conducted in two different months, August and October, by vertical hauling from a depth of 5 meters to the surface. The results revealed a rich diversity of phytoplankton, with 72 genera, 48 families, and 6 classes identified in August, and 55 genera, 42 families, and 6 classes identified in October. The diatom group was the most prevalent, accounting for over 90% of the total, with the highest abundance found in Skeletonema and Chaetoceros. The diversity, uniformity, and dominance indices were all in the low to medium category, indicating a balanced ecosystem

Effective DNA extraction method for metagenomic analysis of rhizosphere bacteria from mangrove sediments Maysaroh, Siti; Ismet, Meutia Samira; Subhan, Beginer; Andini, Rita; Sembiring, Enny Rimita; Anggraini, Nurlita Putri
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan Vol 12, No 2 (2023): AUGUST 2023
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.12.2.32850

Mangrove, Rhizophora mucronata, grows in the intertidal area, which contains much organic matter and varying salinity. The organic matter content is influenced by the bacterial community that inhabits the ecosystem, but information regarding the bacterial community, especially in the mangrove root system, is not widely available. There are several challenges in completing this information, one of which is that the method used is still in a conventional form. Developments in environmental DNA analysis can support and complement this information. However, this method must be optimized because the organic matter content and salt variations affect the extraction results. Thus, this study aimed to determine the optimal approach for extracting bacterial DNA from mangrove sediments. The analysis used two methodologies: manual DNA extraction techniques based on buffer modification and DNA extraction kits. There were four different treatments, namely the soil DNA isolation plus kit (M1), the fecal / soil microbial quick-DNA miniprep kit (M2), glass powder with charcoal (M3), and glass powder with skimmed milk (M4). DNA samples were obtained from each method and assessed for concentration and purity using a nanodrop. In addition, the resulting DNA's quality was analyzed using 1.5% agarose. The results obtained were in the M2 treatment, which showed optimal results compared to the others. M2 uses a bead-based beating and spin column method to achieve optimal DNA concentration through high molecular weight. The DNA obtained was also protein-free, and several samples were contaminated with humic acid, namely KL.S1, KL.S4, and T7.S4.Keywords:Bacteria 16SBead beatingDNA ExtractionSedimentSpin column

Toxicity and antibacterial activity of Rhizophora apiculata mangrove using different solvent extraction : Toksisitas dan aktivitas antibakteri mangrove Rhizophora apiculata dengan pelarut ekstraksi yang berbeda Imanditya, Muhammad Fachriza; Ismet, Meutia Samira; Zamani, Neviaty Putri; Natih, Nyoman MN; Srimariana, Endang Sunarwati; Andini, Rita; Anggraini, Nurlita Putri
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 28 No. 1 (2025): Jurnal Pengolahan Hasil Perikanan Indonesia 28(1)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v28i1.60031

Rhizophora apiculata merupakan jenis mangrove yang banyak digunakan sebagai obat tradisional. Potensi ini dihasilkan oleh kandungan metabolit sekunder yang dapat dipengaruhi oleh kondisi lingkungan, usia, dan beragam faktor lainnya. Penelitian ini bertujuan untuk menentukan pelarut terbaik ekstrak R. apiculate berdasarkan parameter toksisitas dan antibakteri. R. apiculata yang digunakan dalam penelitian ini berasal dari daerah Kuala Langsa dan Pulau Telaga Tujuh (Aceh Timur), dengan ukuran diameter pohon yang berbeda (2,4; 4,8; 5; 13; 23,3; & 26 cm). Ekstraksi dengan pelarut metanol 99,8% (MeOH) dan etanol 99,9% (EtOH) dilakukan dengan maserasi (3x24 jam), ekstraksi dengan pelarut akuades dilakukan dengan perebusan (15 menit pada suhu 85-90°C). Ekstraksi menggunakan perbandingan 10:1 (v/w) dengan 2 g serbuk simplisia dan 20 mL pelarut. Uji toksisitas dilakukan dengan metode brine shrimp lethality assay dan uji antibakteri menggunakan metode disk diffusion assay. Uji toksisitas menunjukkan ekstrak etanol memiliki nilai toksisitas tertinggi dengan nilai LC50 sebesar 26,879 μg/mL. Ekstrak akuades menghasilkan zona hambat tertinggi dengan nilai 11,37±0,85 mm pada bakteri Escherichia coli dan 17,67±1,19 mm pada bakteri Staphylococcus aureus. Hasil ANOVA dua arah menunjukkan pelarut berpengaruh nyata terhadap zona hambat bakteri (p<0,05), sementara diameter pohon tidak berpengaruh nyata (p<0,05). Ekstrak pelarut etanol menghasilkan nilai toksisitas tertinggi, sedangkan ekstrak pelarut akuades menghasilkan zona hambat terbesar.

AKUMULASI MIKROPLASTIK PADA SPESIES IKAN EKONOMIS PENTING DI PERAIRAN PULAU BARRANGLOMPO, MAKASSAR Sawalman, Rahmat; Zamani, Neviaty Putri; Werorilangi, Shinta; Ismet, Meutia Samira
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 13 No. 2 (2021): Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jitkt.v13i2.34587

Mikroplastik (MP) telah ditemukan di berbagai lingkungan laut khususnya pada ikan, dan informasi terkait akumulasi mikroplastik pada organ-organ ikan masih sangat terbatas. Penelitian ini bertujuan untuk membuktikan keberadaan mikroplastik pada organ insang, saluran pencernaan, dan daging ikan ekonomis penting (Hemiramphus far, Siganus virgatus, dan Lethrinus lentjan) di perairan Pulau Barranglompo, Makassar, serta mengidentifikasi karakteristik mikroplastik meliputi warna, bentuk, dan ukuran. Setiap organ diekstraksi menggunakan KOH 20% dan pengamatan karakteristik MP dilakukan secara visual menggunakan mikroskop stereo. Hasil penelitian menunjukkan bahwa mikroplastik ditemukan di insang, saluran pencernaan, dan daging dari ketiga spesies ikan. Tingkat deteksi mikroplastik pada masing-masing H. far, S. virgatus, dan L. lentjan adalah 100%, 100%, dan 82%. Karakteristik warna mikroplastik didominasi oleh biru, bentuk mikroplastik didominasi oleh line, dan kisaran ukuran mikroplastik didominasi 1–5 mm. Penelitian lebih lanjut perlu dilakukan untuk melihat akumulasi mikroplastik pada setiap organ secara eksperimental di laboratorium.

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