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Biodegradation activities of microplastic polymers by Agelas conifera sponge-symbiotic bacteria Meutia Samira Ismet; Endang Sunarwati Srimariana; Nur Alam Dwi Cahyati; Nurlita Putri Anggraini; Lenni Mariana Simbolon; Nadya Cakasana; Mohamad Rafi
Depik Vol 14, No 2 (2025): JUNE 2025
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Research on microplastic (MP) accumulation in marine organisms' tissue have been widely conducted, as well as research on microplastic biodegradation by microbial organisms from the environment.Agelas coniferasponge is proven to accumulate MP in its tissue as well as having bacteria as symbiotic organism. This research aims to screen and study biodegradation activities ofA. coniferasymbiotic bacteria on MP polymers, alsoto identify the species of those bacteria isolates. Bacterial Isolation ofA. coniferasponge tissue was using Zobell 2216E agar media. Screening and biodegradation assay of the bacteria isolates was conducted on Marine Salt Media agar that was added with 0,5 g of MP polymers, using Kroc-Boral method. Diameter of clear (bacterial growth) zone surrounding Kroc-Boral indicated the biodegradation activity of the bacterial isolates. Statistical analysis on the activity was using Two Way ANOVA. Results showed that there were 9 bacterial isolates of 53 that could degrade several MP polymers, with different activities (ANOVA, p value 0.05). High and low-density polymers (Linear- Low Density PolyEthylene/ LLDPE, Polyethylene/PE, and Polyvinyl Chloride/ PVC) were found to be degraded by all 9 isolates. Meanwhile, Polyethylen Taraphtalene (PET) and Polystyrene (PS) only degraded by 8 and 3 isolates, respectively. There are only 5 isolates that have high biodegradation index (1.5) on different polymers, which mostly on PET polymer. Isolate Ac8MP found to be the most bacteria that have degradation index on all polymers more than 1.This research proves that symbiotic bacteria could have a biodegradation mechanism on MP polymers that accumulates by sponge-host. However, further study needs to be done to understand the mechanisms and effectiveness of sponge-bacterial biodegradation activity.
Effective DNA extraction method for metagenomic analysis of rhizosphere bacteria from mangrove sediments Siti Maysaroh; Meutia Samira Ismet; Beginer Subhan; Rita Andini; Enny Rimita Sembiring; Nurlita Putri Anggraini
Depik Vol 12, No 2 (2023): AUGUST 2023
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.12.2.32850

Abstract

Mangrove, Rhizophora mucronata, grows in the intertidal area, which contains much organic matter and varying salinity. The organic matter content is influenced by the bacterial community that inhabits the ecosystem, but information regarding the bacterial community, especially in the mangrove root system, is not widely available. There are several challenges in completing this information, one of which is that the method used is still in a conventional form. Developments in environmental DNA analysis can support and complement this information. However, this method must be optimized because the organic matter content and salt variations affect the extraction results. Thus, this study aimed to determine the optimal approach for extracting bacterial DNA from mangrove sediments. The analysis used two methodologies: manual DNA extraction techniques based on buffer modification and DNA extraction kits. There were four different treatments, namely the soil DNA isolation plus kit (M1), the fecal / soil microbial quick-DNA miniprep kit (M2), glass powder with charcoal (M3), and glass powder with skimmed milk (M4). DNA samples were obtained from each method and assessed for concentration and purity using a nanodrop. In addition, the resulting DNA's quality was analyzed using 1.5% agarose. The results obtained were in the M2 treatment, which showed optimal results compared to the others. M2 uses a bead-based beating and spin column method to achieve optimal DNA concentration through high molecular weight. The DNA obtained was also protein-free, and several samples were contaminated with humic acid, namely KL.S1, KL.S4, and T7.S4.Keywords:Bacteria 16SBead beatingDNA ExtractionSedimentSpin column
Presenting identification keys and future study on seagrass Halophila major in Indonesia Robba Fahrisy Darus; Dietriech Geoffrey Bengen; Neviaty Putri Zamani; Meutia Samira Ismet
Depik Vol 14, No 2 (2025): JUNE 2025
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.14.2.44166

Abstract

Halophila major is a new species with a wide distribution across various regions in Indonesia. The species was identified in 2020 through a combination of morphology and molecular approaches. Several studies have shown that it has a similar morphology to H. ovalis, which causes significant confusion during identification. Therefore, this study aims to describe the key identification and habitat of H. major and summarize seagrasses study opportunities based on the trend of published articles. A bibliometric analysis was used to summarize the habitat, morphometrics, molecular confirmation, and trend of seagrass topics in Indonesia. Based on the scientific articles, H. major has bigger morphometrics than H. ovalis. In addition, the number of paired and branching cross veins was reported to be an identification key of H. major. The species was also considered a deep ovalis found in 2-4 m depth. Genetically, the Internal Transcribed Spacer (ITS) gene marker was appropriate to showits phylogenetic tree. H. major was also classified into different clades with H. ovalis due to various factors. The results showed that genetics, tourism, and restoration were topics with the potential to be explored in the future. This study recommended collaborating in multi-institution to transfer knowledge, technologies, and project arrangements for seagrass exploration.Keywords:BibliometricHalophilaMarine angiospermaeMarin plantRediscoverySpecies
Biodegradation activities of microplastic polymers by Agelas conifera sponge-symbiotic bacteria Meutia Samira Ismet; Endang Sunarwati Srimariana; Nur Alam Dwi Cahyati; Nurlita Putri Anggraini; Lenni Mariana Simbolon; Nadya Cakasana; Mohamad Rafi
Depik Vol 14, No 2 (2025): JUNE 2025
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Research on microplastic (MP) accumulation in marine organisms' tissue have been widely conducted, as well as research on microplastic biodegradation by microbial organisms from the environment.Agelas coniferasponge is proven to accumulate MP in its tissue as well as having bacteria as symbiotic organism. This research aims to screen and study biodegradation activities ofA. coniferasymbiotic bacteria on MP polymers, alsoto identify the species of those bacteria isolates. Bacterial Isolation ofA. coniferasponge tissue was using Zobell 2216E agar media. Screening and biodegradation assay of the bacteria isolates was conducted on Marine Salt Media agar that was added with 0,5 g of MP polymers, using Kroc-Boral method. Diameter of clear (bacterial growth) zone surrounding Kroc-Boral indicated the biodegradation activity of the bacterial isolates. Statistical analysis on the activity was using Two Way ANOVA. Results showed that there were 9 bacterial isolates of 53 that could degrade several MP polymers, with different activities (ANOVA, p value 0.05). High and low-density polymers (Linear- Low Density PolyEthylene/ LLDPE, Polyethylene/PE, and Polyvinyl Chloride/ PVC) were found to be degraded by all 9 isolates. Meanwhile, Polyethylen Taraphtalene (PET) and Polystyrene (PS) only degraded by 8 and 3 isolates, respectively. There are only 5 isolates that have high biodegradation index (1.5) on different polymers, which mostly on PET polymer. Isolate Ac8MP found to be the most bacteria that have degradation index on all polymers more than 1.This research proves that symbiotic bacteria could have a biodegradation mechanism on MP polymers that accumulates by sponge-host. However, further study needs to be done to understand the mechanisms and effectiveness of sponge-bacterial biodegradation activity.
Taxonomy and molecular phylogeny analysis of the genus Diogenes “Edwardsii group” (Decapoda: Anomora: Diogenidae) based on mitochondrial DNA sequences TYANI FITRIAN; HAWIS MADDUPPA; MEUTIA SAMIRA ISMET; DWI LISTYO RAHAYU
Biodiversitas Journal of Biological Diversity Vol. 23 No. 10 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d231040

Abstract

Abstract. Fitrian T, Madduppa H, Ismet MS, Rahayu DL. 2022. Taxonomy and molecular phylogeny analysis of the genus Diogenes “Edwardsii group” (Decapoda: Anomora: Diogenidae) based on mitochondrial DNA sequences. Biodiversitas 23: 5302-5313. Many variations of morphological characters differ from one species to another, showing high interspecific variation within the genus Diogenes Dana, 1851. Subtle morphological differences among certain species make it difficult to identify diagnostic characters. Although the genus is very speciose, the study on the molecular is poorly reported. This study aimed to evaluate the taxonomy and phylogeny of the genus Diogenes using morphological and molecular features. The molecular phylogeny of 15 species of Diogenes included in the "Edwardsii group" was constructed based on two mitochondrial genes COI and 16S rRNA. The results of the phylogeny tree construction based on concatenated mitochondrial genes COI and 16S rRNA mtDNA formed 5 clades of monophyletic. Phylogenetic relationships of Diogenes species based on concatenated mitochondrial genes COI and 16S rRNA sequenced suggest that the morphological and molecular analyses of the species in this study corroborated one another. Among 15 species studied, three species were challenging to identify due to their resemblance to other species traits. The phylogenetic tree demonstrates that the three taxa denoted by 'sp.' were different from the known species of Diogenes so far. Diogenes sp.1 was closely related to Diogenes moosai Rahayu & Forest, 1995, Diogenes sp.2 resembles Diogenes laevicarpus Rahayu, 1996, while Diogenes sp.3 was closely related to Diogenes goniochirus Forest, 1956.
Heavy metals concentration in sediment and their bioaccumulation in several organisms in Benoa Bay and Lembongan Island, Bali, Indonesia PUTU YUDI ADITYA PUTRI; RASTINA RASTINA; TRI PRARTONO; MEUTIA SAMIRA ISMET
Biodiversitas Journal of Biological Diversity Vol. 25 No. 7 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250744

Abstract

Abstract. Putri PYA, Rastina, Prartono T, Ismet MS. 2024. Heavy metals concentration in sediment and their bioaccumulation in several organisms in Benoa Bay and Lembongan Island, Bali, Indonesia. Biodiversitas 25: 3230-3238. Human activities could be responsible for introducing heavy metals into coastal areas, and it can contaminate coastal waters such as the tourism area of Benoa Bay and coastal ecosystems of Lembongan Island, Bali, Indonesia. This study aimed to assess the extent of heavy metals on the sediment of those area and their accumulation rate in several marine organisms, including macro-algae (Ulva sp.), seagrass (Thalassia hemprichii), and sea urchin (Diadema sp.). Samples of surface sediments and marine organisms were collected at six stations for both areas and the presence of five heavy metals (Fe, Pb, Cd, Cu, and Cr) was determined using the AAS method. The concentration those metals varied significantly across the six stations. The geo-accumulation index showed that the sediment has been contaminated by Pb and Cd. The ecological risk of heavy metals contamination in Benoa Bay was apparently higher compared to that in Lembongan Island. Marine organisms in Benoa Bay also significantly accumulated heavy metals than those observed in Lembongan Island. Bioconcentration factor (BCF) shows that Cd, Cr, and Pb were the most accumulated heavy metals in marine organisms (BCF>1). It, therefore, suggest that future studies of the bioavailability of heavy metals and exploration the varying capacities of marine organisms are required. Such important information can be used to predict the accumulation of heavy metals in human bodies, and the possibly daily consumption of marine organisms caught from Benoa Bay and Lembongan Island waters.
Using morphometric characters to estimate leaf productivity of seagrass Halophila major Robba Fahrisy Darus; Dietriech Geoffrey Bengen; Neviaty Putri Zamani; Meutia Samira Ismet
Biodiversitas Journal of Biological Diversity Vol. 25 No. 12 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d251237

Abstract

Abstract. Darus RF, Bengen DG, Zamani NP, Ismet MS. 2024. Using morphometric characters to estimate leaf productivity of seagrass Halophila major. Biodiversitas 25: 4994-5004. Halophila major is a seagrass species with new records in Indonesia. This species was described based on morphometric characteristics and DNA sequencing. In this study, we expand our understanding about this species by looking at its leaf productivity. This study aims to determine the productivity of H. major leaves collected across various locations of Indonesian waters and analyses its relationship with morphometric characters. Morphometric characters (Leaf Length-LL, Leaf Width-LW, and Leaf Area-LA) were used to see the correlation with leaf productivity parameters, namely Leaf Dry Weight (LDW), Specific Leaf Area (SLA), Biomass (BIO), and Carbon Concentration (CS) estimated using allometric methods. The result of this study indicates that the biomass of H. major is greater than H. ovalis, its closest relative. LL and LW were more strongly correlated with LDW compared to LA. Biomass was also greatly influenced by LL and LDW. Leaf productivity variation is affected by the leaf size, suggesting that leaf productivity can be used to distinguish seagrass species under the similar genus. This study showed that allometric equations can be used quickly to assess the biomass of small seagrasses. However, more data is needed for knowing and interpreting a character in new seagrass species. Hence, this study must be replicated for other seagrass species.
Karakterisasi Komunitas Bakteri Indikator di Tambak Udang untuk Mengevaluasi Kesehatan Media Budidaya Hani Fattus Sabrina Anam; Ida Bagus Jelantik Swasta; Anak Agung Ayu Agung Widhyani; Meutia Samira Ismet; Ketut Mahardika; Gede Iwan Setiabudi
Vol 16 No 4 (2026): JURNAL PERIKANAN
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jp.v16i4.2414

Abstract

EFLUEN TAMBAK UDANG TERHADAP MIKROBIOMA PESISIR DAN IMPLIKASINYA TERHADAP KUALITAS AIR SERTA PROLIFERASI PATOGEN Ni Kadek Martha Ayu Saraswati; Jasmine Masyitha Amelia; Anak Agung Ayu Agung Widhyani; Meutia Samira Ismet; Ketut Mahardika; Gede Iwan Setiabudi
Vol 16 No 4 (2026): JURNAL PERIKANAN
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jp.v16i4.2421

Abstract

Shrimp aquaculture faces significant challenges from disease outbreaks and environmental degradation caused by cultivation waste. This study aims to characterize the indicator bacteria communities in the coastal areas surrounding shrimp farms and to evaluate their correlation with water quality parameters as a tool for monitoring ecosystem health. This research employed a descriptive-observational approach and conventional cultivation on selective media, including Thiosulfate-Citrate-Bile Salts-Sucrose (TCBS) Agar and Pseudomonas Isolation Agar, to quantify the abundance of Vibrio spp. and Pseudomonas spp., respectively, alongside Marine Agar. Measurements of water quality parameters such as temperature, salinity, pH, dissolved oxygen (DO), nitrate, nitrite, and ammonia were also integrated for correlation analysis. The analysis revealed that aquaculture effluent significantly increased the abundance of total heterotrophic bacteria and Vibrio spp. in the coastal waters receiving farm waste. The abundance of Vibrio was positively correlated with temperature and pH, and negatively correlated with dissolved oxygen. Bacterial communities, including Vibrionaceae and Pseudomonadaceae, were identified as core components of the microbiome in waters exposed to anthropogenic waste. Fluctuations in environmental parameters trigger shifts in these bacterial communities, elevating the risk of pathogen and toxin presence. These findings underscore the importance of monitoring indicator bacteria for sustainable farm management, mitigating disease risks, and ensuring the suitability of coastal waters as a viable medium for aquaculture.
Co-Authors Abrahamsz, Jodie G. Adriani Sunuddin Agung Putra Utama Ajie Imamsyah Alfaiz Arselan Anak Agung Ayu Agung Widhyani Anak Agung Ayu Agung Widhyani Anggraini, Nurlita Putri Arisandi, Defrian Marza Ayu, Inna Puspa Aziz, Nasrul Aznardi, Shodikin Aznardi Beginer Subhan Dea Fauzia Lestari, Dea Fauzia Dedi Soedharma Dietriech Geoffrey Bengen Dietriech Geoffrey Bengen Dietriech Geoffrey Bengen Dondy Arafat DWI LISTYO RAHAYU Endang Sunarwati Srimariana Enny Rimita Sembiring Fadel Muhammad Fery Kurniawan Gede Iwan Setiabudi Gede Iwan Setiabudi Hamdanul Fain Hani Fattus Sabrina Anam HAWIS MADDUPPA Hawis H Madduppa Hefni Effendi Husmayani, Wa Ode I Ketut Mahardika Ida Bagus Jelantik Swasta Ilham, Yuwanda Imanditya, Muhammad Fachriza Jasmine Masyitha Amelia Kadek Lila Antara Kesha Mufthi Java Tanaya Ketut Mahardika Lenni Mariana Simbolon Maestro Munru Miswandi, Muhammad Mohamad Rafi Muhammad Irlan Assidiq Kusuma Ramadhan Muhammad Izzat Nugraha Muhammad Reza Cordova, Muhammad Reza Mujizat Kawaroe Munru, Maestro Nadya Cakasana Nasrul Aziz Neviaty Putri Zamani Neviaty Putri Zamani NEVIATY PUTRI ZAMANI Ni Kadek Martha Ayu Saraswati Noferya, Efi Nur Alam Dwi Cahyati Nurlita Anggraini Nurlita Putri Anggraini Nyoman M N Natih Ocky Karna Radjasa PUTU YUDI ADITYA PUTRI Radixa Radhya Ramadhan Haryanto Rahman, Lucky Rahmat, Fadhlan Basiluddin RASTINA RASTINA Retraubun, Alex S.W. RITA ANDINI Rita Andini Rita Andini Robba Fahrisy Darus Robba Fahrisy Darus Robba Fahrisy Darus, Robba Fahrisy Ryan Adhitia Muslim Sallatu, Muh Ashry Salsabila, Afviya Samsuardi Samsuardi, Samsuardi Sawalman, Rahmat Setyaningsih, Wahyu Adi Shinta Werorilangi, Shinta Simatupang, Candida Putri Nauli Siti Maysaroh Siti Maysaroh Taqiyuddin, Muhammad Wijdan TRI PRARTONO TYANI FITRIAN Yennafri, Yennafri yoman Metta N Natih Zakiyah Rahim