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Dhiah Novalina
program Studi Biosain PPs UNS
Aerospace Engineering Agriculture, Biological Sciences & Forestry Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Economics, Econometrics & Finance Engineering Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Journal : Borneo Journal of Medical Laboratory Technology

 1 
Potensi Dan Uji Stabilitas Ekstrak Lawsonia Inermis Sebagai Cat Penutup Pada Gram Staining Dengan Variasi Suhu: Potency and Stability Test of Lawsonia inermis Extract as Counterstain on Gram Staining with Temperature Variation Asfiya, Nida Azki; Novalina, Dhiah; Astuti, Tri Dyah
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 2 (2024): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i2.6736

Abstract

Gram staining is one of the methods used for bacterial identification. Safranin is one of the dye substances employed in Gram staining. The toxicity and health hazards associated with the use of safranin have been extensively reported, especially the risk of contamination from synthetic dye waste. The use of synthetic dyes is highly hazardous as it can lead to cancer, kidney, and liver damage. A potential alternative for Gram staining is the use of henna leaves (Lawsonia inermis), which contain the dye substance lawsone. However, natural dyes are susceptible to temperature changes.This research aims to determine the ability of henna leaves to stain bacteria as a counterstain in Gram staining and to assess the temperature stability of the henna leaf extract under various temperature variations. This study is a qualitative descriptive research with an experimental design conducted in the Microbiology Laboratory of the Faculty of Health Sciences at 'Aisyiyah University Yogyakarta. The results of Gram staining for Gram-negative bacteria (Escherichia coli) at temperatures of 40°C and 80°C show no contrast with the background, while Gram-positive staining for Staphylococcus aureus appears contrasting against the background. It can be concluded that the Gram staining of Escherichia coli bacteria with Lawsonia inermis extract at temperatures of 40°C and 80°C yields staining results that lack contrast between bacterial cells and the background. In contrast, Staphylococcus aureus bacteria exhibit contrasting staining results between bacterial cells and the background. The stability test of Lawsonia inermis extract indicates greater stability at 40°C compared to 80°C
Analisis Quality Control (QC) Pemeriksaan Albumin Dan Bilirubin Menggunakan Kontrol Harian Dan Matriks Sigma: Quality Control Analysis Of Albumin And Bilirubin Testing Using Daily Controls And Sigma Matrix Rahmani, Afina Rusyda; Amalia, Arifiani Agustin; Novalina, Dhiah
Borneo Journal of Medical Laboratory Technology Vol. 7 No. 1 (2024): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v7i1.7863

Abstract

Quality control (QC) merupakan suatu proses evaluasi pengujian mutu laboratorium yang bertujuan untuk menjamin sistem mutu laboratorium. Six sigma merupakan indikator kualitas yang digunakan untuk mengevaluasi kinerja analitik. Penelitian ini bertujuan untuk mengetahui nilai data kontrol harian melalui evaluasi grafik Levey-jennings dan mengetahui nilai sigma beserta DPMO pada pemeriksaan albumin dan bilirubin total. Metode penelitian yang digunakan yaitu metode deskriptif kuantitatif dengan pendekatan penelitian cross sectional. Metode pengambilan sampel pada penelitian ini menggunakan Teknik total sampling. Populasi pada penelitian ini yaitu data sekunder kontrol harian dari pemeriksaan albumin dan bilirubin bulan Oktober 2023 – Maret 2024. Berdasarkan hasil evaluasi grafik Levey-jennings dengan aturan westgard pada pemeriksaan albumin dan bilirubin terdapat nilai kontrol yang melanggar aturan 12s, 13s, dan 41s selama enam bulan berturut-turut. Pada pemeriksaan albumin terdapat nilai bias PME 2,07%, rata-rata CV PMI 1,46%, serta nilai sigma 5,4. Pada pemeriksaan bilirubin total terdapat nilai bias PME 7,79%, rata-rata CV PMI 2,78%, serta nilai sigma 4,4. Hasil evaluasi grafik Levey-jennings dengan aturan westgard pada pemeriksaan albumin dan bilirubin terdapat nilai kontrol yang melanggar aturan 12s, 13s, dan 41s. Nilai sigma pemeriksaan albumin 5,4 dengan DPMO 40 dan pada pemeriksaan bilirubin total 4,4 dengan DPMO 1.860. Quality control (QC) is a process for evaluating laboratory testing quality to ensure the integrity of the laboratory's quality system. Part of this process involves using Six sigma, a quality indicator that assesses analytical performance. This study aims to determine the value of daily control data through the evaluation of the Levey-jennings chart and determine the sigma value and DPMO in the albumin and total bilirubin of testing. This study used quantitative descriptive method with cross-sectional approach. The sampling method employed was total sampling. The population composed of secondary data from daily controls of albumin and bilirubin tests conducted October 2023 – March 2024. Based on the evaluation of the Levey-jennings chart using Westgard rules for albumin and bilirubin tests, control values violating the 12s, 13s, and 41s rules for six consecutive months. For albumin testing, the PME bias was 2.07%, the average CV PMI was 1.46%, and the sigma value was 5.4. For total bilirubin testing, the PME bias was 7.79%, the average CV PMI was 2.78%, and the sigma value was 4.4. In conclusion, the evaluation of the Levey-jennings chart using Westgard rules showed that the control values for albumin and bilirubin tests violated the 12s, 13s, and 41s rules. The sigma value for albumin testing was 5.4 with a DPMO of 40, while for total bilirubin testing, it was 4.4 with a DPMO of 1.860.
Rhodamine B Sebagai Pewarna Alternatif Carbol Fuchsin Pada Perwarnaan Tahan Asam Ziehl Neelsen : Rhodamine B as an Alternative to Carbol Fuchsin in Acid-Fast Staining Using the Ziehl-Neelsen Method Fauziyah, Fitri Akbar; Novalina, Dhiah; Putri, Novita Eka
Borneo Journal of Medical Laboratory Technology Vol. 7 No. 1 (2024): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v7i1.7932

Abstract

Bakteri merupakan mikroorganisme yang umumnya tidak memiliki warna dan sulit untuk melihat atau mengidentifikasi bentuk selnya di bawah mikroskop. Oleh karena itu, dilakukan pengecatan atau pewarnaan sel bakteri untuk memperjelas bentuknya dan memudahkan identifikasi sel bakteri. Penelitian ini bertujuan untuk mengetahui potensi rhodamine B sebagai pewarna alternatif carbol fuchsin pada pewarna Bakteri Tahan Asam metode Ziehl neelsen. Metode yang digunakan ialah menggunakan metode Quasi eksperimen dengan desain nonequivalent control group design yaitu dengan adanya kelompok eksperimen dan kontrol. Penelitian dilakukan dengan menggunakan data primer di Rumah Sakit Umum Daerah Wates. Hasil penelitian ini menunjukan bahwa data skor kriteria pewarnaan pada 24 sediaan dengan 4 perlakuan dan 6 pengulangan adalah pada kontrol positif didapatkan jumlah skor 150, pada rhodamine B 4% pelarut aquades dan etanol didapatkan skor 90 dengan nilai presentase efektivitas didapatkan 60% dan untuk rhodamine B 3,98% pelarut aquades yang ditambahkan fenol didapatkan jumlah skor 150 dengan nilai persentase efektivitas 100%. Berdasarkan penelitian yang dilakukan rhodamine B 4% pelarut aquades dan etanol tidak efektif digunakan sebagai alternatif pengganti carbol fuchsin sedangkan hasil penelitian rhodamine B 3,98% yang dilarutkan menggunakan aquades dan ditambahkan fenol efektif digunakan sebagai pewarna alternatif carbol fuchsin.
Co-Authors Aliffani, Muthi’ah Fadhilah Anandarianto, Annisa Shafa Anggraeni, Rosmita Aprianto, Muhammad Eko ARI SUSILOWATI Arifiani Agustin Amalia Asfiya, Nida Azki Astuti, Tri Dyah Bagus Widyantara, Aji Bara, Maria Kornelia Damayanti, Sunita Fauziana, Dwi Ari Fauziyah, Fitri Akbar Hadi, Wahid Syamsul Irfani, Farida Noor Kasim, Allya Nurkhairunnisa Kumalasari, Dei Mabruroh, Indah Nahdotul Permata Sari, Diah Putri, Novita Eka Putri, Suci Primulya Rahmani, Afina Rusyda Rahmasari, Nely Sri Martuti Sugiyarto Sugiyarto Syamsul Hadi, Wahid Triani, Andi Rizkita Umimi, Kamelia Widyaningtias, Septy Widyantara, Aji Bagus Wiwit Probowati Yeni Rahmawati, Yeni