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All Journal Microbiology Indonesia Biomedical Engineering Jurnal Florea Al Ishlah Jurnal Pendidikan JPM (Jurnal Pemberdayaan Masyarakat) BIOEDUSCIENCE Jurnal SOLMA Jurnal Penelitian dan Pengkajian Ilmu Pendidikan: e-Saintika Bubungan Tinggi: Jurnal Pengabdian Masyarakat Indian Journal of Forensic Medicine & Toxicology Berkala Penelitian Hayati Media Didaktika Journal of Biodiversity and Biotechnology Jurnal Penelitian Pendidikan Indonesia (JPPI) Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Edumaspul: Jurnal Pendidikan
Supiana Dian Nurtjahyani
Universitas PGRI Ronggolawe
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Other

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Identification and Characterization of Pathogenic Fungi Causing Necrosis in Vigna unguiculata Afiya, Dina; Mustofa, Ali; Nurtjahyani, Supiana Dian
BIOEDUSCIENCE Vol 10 No 1 (2026): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/18508

Abstract

Background: Vigna unguiculata (cowpea) is an important legume crop whose productivity can be affected by foliar diseases, including leaf necrosis caused by fungal pathogens. Identification of fungi associated with necrotic symptoms is essential as baseline information for plant disease management. Methods: This study aimed to identify fungi associated with necrotic lesions on cowpea leaves. Leaf samples showing necrotic symptoms were collected from agricultural fields in Tengger Wetan Village, Kerek Subdistrict, Tuban Regency, East Java. Fungal isolation was performed from infected leaf tissues to obtain pure cultures. Identification was conducted based on macroscopic colony characteristics and microscopic features using slide culture techniques and standard morphological identification keys. Results: Two dominant fungal isolates were consistently associated with necrotic symptoms on cowpea leaves. These fungi were identified morphologically as Alternaria alternata (isolate D1) and Nigrospora sp. (isolate D3). A. alternata was characterized by yellowish-white colonies, hyaline septate hyphae, and dark oval conidia, whereas Nigrospora sp. exhibited cottony white colonies with brown, globose conidia. Both fungi were associated with brown to black necrotic spots on the leaf surface. Conclusions: The findings provide initial information on fungal species associated with leaf necrosis in cowpea based on morphological identification. This information is useful as a foundation for further pathological and molecular studies to confirm species identity and support the development of appropriate disease management strategies.
Isolation and Identification of Arbuscula Mycorrhiza (Ma) in Plant Rhizosphere in Greenbelt Area of PT. Semen Indonesia Tbk Tuban Avivi Nur Aina; Supiana Dian Nurtjahyani; Sriwulan Sriwulan; Eko Purnomo
Journal of Biodiversity and Biotechnology Vol 3, No 1 (2023)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v3i1.72038

Abstract

The Green Belt area is a buffer area in an industrial area that functions as a barrier or separates the mining area from other regions, such as residential areas or community agriculture. The Green belt area consists of various plant vegetation that absorbs carbon dioxide and air conditioning, preventing sedimentation and erosion in the mining area. Arbuscular mycorrhiza has the potential to live in the green belt area by forming a mutualism symbiosis with plants or vegetation in the area. This study aimed to determine arbuscular mycorrhizae found in the Greenbelt area of PT Semen Indonesia Tbk Tuban. Sampling used purposive sampling techniques at three sampling stations, namely rhizosphere eucalyptus (Malaleuca cajuputi), trembesi (Samanea saman), and sawo (Manilkara zapota). The study was conducted in November 2021 through three stages: sampling, sample isolation, and species identification. The results showed seven types of arbuscular mycorrhiza from five different genera, namely the genus Glomus, Acaulospora, Gigaspora, Racocetra, and Archaeospora. The most found species is the genus Glomus. This research contributes as a source of literacy and information about the types of AMF found in PT Semen Indonesia's Greenbelt area, which can be used as biological fertilizer, support plant growth, help reclaim mining areas, and protect plants from drought pressure in mining areas.
Pelatihan Bertanam Sayuran dengan Teknik Vertikultur pada Paguyuban Wali Murid Raudhotul Athfal Thoriqul Falah Dede Nuraida; Tabitha Sri Hartati Wulandari; Supiana Dian Nurtjahyani; Sulistiono
Jurnal Pemberdayaan Masyarakat Vol 8 No 1 (2023): Mei
Publisher : Direktorat Penelitian dan Pengabdian kepada Masyarakat (DPPM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21067/jpm.v8i1.7509

Abstract

Raudhotul Athfal (RA) Thoriqul Falah is located in Talang Kembar Village. Mothers in Talang Kembar Village are generally unproductive housewives, so there needs to be empowerment to be more productive. The purpose of this training is to provide skills for the parents of the RA Thoriqul Falah Student, in making gardening places using vertical culture, making media, as well as selecting and planting seeds. This training activity is carried out in 2 methods, namely socialization about verticulture, and practice. The socialization method was carried out in the RA meeting room, while the practice was carried out in the school yard. The vertical planting is made from used mineral water bottles and paralon. The vegetables used were lettuce, pokcoy, and celery grown in a mixture of soil, husk and manure in a ratio of 2:1:1. Questionnaire given to all participants to find out the participants' interest in applying this technique. The results of this training showed that all participants were able to make vertical planting. All participants were able to make a composition of planting media, and were able to select and plant seeds. The results of the questionnaire showed that 90% of the participants were interested in applying this technique.
Co-Authors *Ali Mustofa Afiya, Dina Agusti Agusti Ahmad Yogi Pambudi Ahmad Zainal Arifin Ainur Rofieq Ali Mustafa Ali Mustofa Anik Winarni Arga Dias Pratama Dias Pratama Arifin, Ahmad Zaenal Avivi Nur Aina Christina Innocenti Tumiar Pangabean Christina Innocenti Tumiar Panggabean Dede Nuraida Devi Shintya Agustin Devi Shyntya Agustin Dhiaulhaq, M. Rafi Dwi Oktafitria Dwi Oktafitria Dwi Oktafitria Dwi Oktavitria Eko Purnomo Eko Purnomo eko purnomo Eko Purnomo Eko Purnomo Eko Purnomo Endah Mutiara Marhaeni Putri Endri Ulfa Kusmawati IS Fajrina Zulfa Darumiarsi Gea Elina Handajan, Retno Hanifa Aasaa Mufida Heny Sulistyaningrum Imas Cintamulya Indah Yana Indra Sugiarsi Kholisotul Hidayah Miftahus Surur MOCHAMAD AMIN Mochammad Amin Mohammad Amin Muhammad Nadjib Mujahid Nia Nurfitria Nurfitria Nova Maulidina Nova Maulidina Nugroho Budi Utomo Nugroho Budi Utomo Nurrohman Nurrohman Nurrohman, Endrik Nurul Hidayah Rafwan Year Perry Burhan Restiani Agusvita Retno Handajani Riris Suharti Lestari Roimil Latifa Rr. Eko Susetyarini Ruril Rudianto Spanton, Perdana Ixbal Sri Wulan Sri Wulan Sriwulan Sriwulan Sriwulan, Sriwulan Sukisno Sulistiono Sulistiono Tabitha Sri Hartati Wulandari Tegar Kurnia, Dwi Wahyu Nur Susanto Zuli Nofiyanti