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ISOLASI PROTOPLAS TANAMAN KACANG HIJAU (Vigna radiata (L.) Wilczek) SECARA ENZIMATIK DENGAN KOMPOSISI ENZIM BERBEDA Mohamad Nurzaman; Tia Setiawati
BIOTIKA Jurnal Ilmiah Biologi Vol 1, No 1 (2002): Biotika Juni 2002
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/bjib.v1i1.149

Abstract

The effects of different enzyme compositions and age of explants on protoplasts isolation  of mungbean (Vigna radiata (L) Wilczek) had been carried out. Protoplasts were isolated from the first leaves of 5, 6, 7, and 8 days old of mungbean seedling, which were incubated in 5 types enzyme composition for 4 hours. The results showed that the yield of protoplasts more than 106 protoplas/g leaf for all combinations. The highest yield of  protoplasts (3,4 x 107 protoplasts/g leaf) were obtained from 7 days old seedling using a combination of 4% Meicelase P-1, 1% Macerozyme R-10 and 0.5 M mannitol. From these results, it can be concluded that the type of enzyme compositions and age of explants influent the yield of protoplasts isolation
PEMANFAATAN TUMBUHAN SEBAGAI OBAT OLEH MASYARAKAT KAMPUNG ARENG KABUPATEN BANDUNG BARAT Asep Zainal Mutaqin; Mohamad Nurzaman; Tia Setiawati; Ruly Budiono; Azifah An’amillah; Johan Iskandar
BIOTIKA Jurnal Ilmiah Biologi Vol 14, No 1 (2016): BIOTIKA JUNI 2016
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/bjib.v14i1.14411

Abstract

Penelitian ini dilakukan untuk mendokumentasikan pengetahuan masyarakat Kampung Areng Desa Wangunsari Kecamatan Lembang Kabupaten Bandung Barat Propinsi Jawa Barat mengenai jenis-jenis tumbuhan yang dimanfaatkan sebagai obat tradisional. Metode yang digunakan untuk penelitian ini adalah metode kualitatif bersifat deskriptif analisis. Teknik pengumpulan data dilakukan dengan cara observasi partisipatif dan wawancara semi struktur terhadap informan kunci. Penentuan informan dilakukan dengan teknik snowball sampling. Hasil wawancara menunjukkan bahwa terdapat 63 spesies tumbuhan yang digunakan sebagai obat tradisional oleh masyarakat Kampung Areng. Tumbuhan-tumbuhan obat tersebut diperoleh masyarakat dari kebon tegal, kebon, dan pekarangan. Masyarakat mengolah tumbuhan obat dengan berbagai cara, di antaranya adalah dengan cara direbus, dicincau (diperas), dibuat ramuan, ditempel, dibalur, dan dimakan langsung.
HUBUNGAN KARAKTERISTIK DAUN DENGAN AKUMULASI PARTIKULAT DAN TIMBAL (Pb) PADA DAUN POHON Pterocarpus indicus Willd., Swietenia macrophylla King., dan Filicium decipiens (Wight & Arn) Thwaites DI KAWASAN TAMAN LANSIA KOTA BANDUNG Julianty Nur C.; Mohamad Nurzaman; Asep Zainal Mutaqin
BIOTIKA Jurnal Ilmiah Biologi Vol 13, No 2 (2015): BIOTIKA DESEMBER 2015
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/bjib.v13i2.10108

Abstract

STUDI MORFOLOGI BEBERAPA JENIS LAMUN DI PANTAI TIMUR DAN PANTAI BARAT, CAGAR ALAM PANGANDARAN Tia Setiawati; Maryam Alifah; Asep Zainal Mutaqin; Mohamad Nurzaman; Budi Irawan; Ruly Budiono
Jurnal Pro-Life Vol. 5 No. 1 (2018): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Sea grass is one component of biodiversity in marine waters composed of many species with various morphological characters. The aim of this study was to obtain the morphological data of seagrass species on the East and West Coasts in Nature Reserve of Pangandaran. The method used was survey method and line transect as far as 200 meters with random sampling technique. Measurements were made for each species of seagrass in the leaf length, leaf width, leaf thickness, stem length and rhizomes length and root length. The results showed that on the East Coast founded three species of seagrass were Cymodocea rotundata, Thalassia hemprichii and Halodule pinifolia, while on the West Coast founded one species of seagrass was Thalassia hemprichii. Cymodocea rotundata has characteristic rounded edges with slight curvature in the middle, Thalassia hemprichii has rounded, brown tip and a brown patterned rhizome, and Halodule pinifolia has side toothed leaf tip with part of the middle there are brown leaf veinsKey words: sea grass, morphology, Pangandaran Nature Reserve
RESPON PERTUMBUHAN STEK CABANG BAMBU AMPEL KUNING (Bambusa vulgaris Schard.Ex Wendl.var. Striata) DENGAN PEMBERIAN ZAT PENGATUR TUMBUH NAA (Naphthalein Acetic Acid) DAN Rootone F Tia Setiawati; Noviyanti Soleha; Mohamad Nurzaman
Jurnal Pro-Life Vol. 5 No. 3 (2018): November
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Yellow ampel bamboo is one type of bamboo that has economic value. One effort that can be done for maintaining the existence of bamboo population so that its can be sustainably used through the cultivation of branch cuttings. The success growth of branch cutting can improve with using plant growth regulator (PGR), such as NAA and Rootone F. The aim of this study was to get the best kind and concentration of PGR that can promote the growth of ampel yellow bamboobranch cutting. This study used experimental methods with a completely randomized design (CRD) two factors. The first factor wasthe kind of PGR which consists of two levels: z1 = NAA and z2 = Rootone F. The second factor was the concentration of PGR which consists of six levels: k1 = 0 ppm, k2 = 100 ppm, 200 ppm = k3, k4 = 300 ppm, 400 ppm = k5, and k6 = 500 ppm. Data were analyzed using ANOVA (α = 5%) and to know the difference between treatments used Tukey test (α= 5%). The results showed that the Rootone F had a better effect than NAA on the growth of yellow bamboo branch cutting. Concentration of 200 ppm was the best concentration to the growth for branch cuttings of yellow ampel bamboo. Interaction Rootone F and concentration 200 ppm showed the best result to the growth for branch cuttings ampel bamboo.Keywords :branch cutting, ampel yellow bamboo, NAA, Rootone F
INDUKSI KALUS KRISAN (Chrysanthemum morifolium Ramat var. Tomohon Kuning) DENGAN 2,4-Dichlorophenoxyacetic Acid (2,4-D) DAN 6-Benzylaminopurine (BAP) PADA KONDISI PENCAHAYAAN BERBEDA Tia Setiawati; Annisa Nur Arofah; Mohamad Nurzaman
Jurnal Pro-Life Vol. 7 No. 1 (2020): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

This study aims to obtain the optimum concentration of 2,4-D and BAP plant growth regulators (PGRs) to induceChrysanthemum callus in light and dark conditions. The method used is an experimental method in the laboratoryusing a Completely Randomized Design (CRD) with 4 treatment of 2,4-D + BAP concentration combinations. Theculture was incubated under different lighting conditions for 45 days after planting. The parameters observedincluded ctexture and color of callus, other responses produced by explants, size, fresh weight and dry weight ofcallus. Data were analyzed descriptively. The results showed that 4 ppm 2,4-D + 0.5 ppm BAP treatment was thebest combination in inducing Chrysanthemum callus in both light and dark conditions. In bright conditions, mostof the callus were dark green and dark brown with a compact texture, callus size of 1.36 cm, and the highest freshweight and dry weight of callus were 0.62 gram and 0.17 gram respectively. Meanwhile, in the dark conditionsmost of the callus were light green and light brown with a compact texture, callus size 1.18 cm, and the highestfresh weight and dry weight of the callus produced were 0.51 grams and 0.15 grams, respectively. Keywords: Callus, Chrysanthemum morifolium, 2,4-Dichlorophenoxyacetic acid (2,4-D), 6-Benzylaminopurine(BAP)
RESPON PERTUMBUHAN KAPULAGA LOKAL (Amomum compactum Soland Ex. Maton) DAN KAPULAGA SABRANG (Elettaria cardamomum (L.) Maton Var. Mysore) TERHADAP CEKAMAN KEKERINGAN Mohamad Nurzaman; Sri Ratu Dewi Pridani; Tia Setiawati
Jurnal Pro-Life Vol. 7 No. 1 (2020): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Drought stress is an inhibiting factor in agricultural, one of the effort to overcome these problems is to use varietiesof plants that are tolerant to drought stress. Cardamom is a plant of the Zingiberaceae which is able to grow indrought stress. This research aims to obtain the growth response of local Javanese cardamom (A. compactum)and true cardamom (E. cardamomum) and to obtain the kind of cardamom that is tolerant to drought stress. Thisresearch was conducted using experimental method and completely randomized design (CRD) with 2 x 4 factorialand four repetitions. The first factor was the variation of field capacity (K) and the second factor was the type ofcardamom (J). Parameters measured include the increase of plant height (cm), plant weight (grams), leaf area(cm2), number of leaves, leaf chlorophyll content (mg / l), and leaf proline content (ppm). The data was analysedusing analysis of variance (ANOVA) and Duncan test α 5%. Results showed that local Javanese cardamom (A.compactum) has better growth response and was more tolerant to drought stress compared to true cardamom (E.cardamomum). Keywords: drought stress, local Javanese cardamom (A. compactum), true cardamom (E. cardamomum).
ANALISIS PERTUMBUHAN DAN KANDUNGAN TOTAL FLAVONOID KULTUR KALUS KRISAN (Chrysanthemum morifolium Ramat) DENGAN PEMBERIAN ASAM 2,4-DIKLOROFENOKSIASETAT (2,4-D) DAN AIR KELAPA Tia Setiawati; Anggita Levi Astuti; Mohamad Nurzaman; Nining Ratningsih
Jurnal Pro-Life Vol. 8 No. 1 (2021): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Chrysanthemum morifolium contains flavonoids which have antioxidant properties and was useful in treating various diseases. The production of flavonoids from plants can be done in vitro through callus culture. Auxin 2,4-D growth regulators and organic compounds such as coconut water added to the media can affect the success of callus formation and secondary metabolite synthesis. The purpose of this study was to obtain the best treatment of 2,4-D concentration and coconut water that can increase the growth and total flavonoid levels of C. morifolium callus cultures. The method used in this research was the experimental method completely randomized design (CRD) with 8 treatments, namely 1 ppm 2,4-D + 10% coconut water (CW); 2 ppm 2,4-D + 10% CW; 3 ppm 2,4-D + 10% CW ; 4 ppm 2,4-D + 10% CW; 1 ppm 2,4-D; 2 ppm 2,4-D; 3 ppm 2,4-D; 4 ppm 2,4-D in 4 replication. The results showed that the treatment of 4 ppm 2,4-D and 10% CW was the best treatment combination, resulting in an average callus size (1.4 cm), fresh weight (0.19 grams), dry weight (0.16 gram) and total flavonoid content (1.873 mgQE/g)
Pemberian Asam Humat untuk Meningkatkan Pertumbuhan dan Kandungan Total Flavonoid Sawi Hijau (Brassica juncea L.) pada Perbedaan Kadar Salinitas Tia Setiawati; Tentani Buhti Amadea; Mohamad Nurzaman; Nining Ratningsih
Jurnal Pro-Life Vol. 8 No. 3 (2021): November
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Salt affects plant growth due to increased soil osmotic pressure and plant nutrients disturbance. Most plants are susceptible to high salinity which can cause physiological and biochemical interference. This research was conducted to obtain a possible salinity level for mustard greens (Brassica juncea L.) to grow well and the best humic acid dosage to reduce salinity damages and to gain high total flavonoid content. This research used Randomized Block Design method with two factors and four replications. The factors are salinity levels (control, 50, 75, and 100 mM) and humic acid dosage (control, 2, 4, and 8 g/kg). In the parameters which had interactions between the two treatments, the highest results were obtained with a combination of 100 mM salinity and 12 g humic acid, such as plant height (33.35 cm), leaf area (379.66 cm2), fresh weight (22.41 g) and dry weight (1.54 g). The highest results on leaf number (8.5 leaves) and total flavonoid content (0.074 mgQE/g sample) were obtained with 100 mM salinity. Humic acid dose of 12 g gave the highest result on leaf number (8.69 leaves) and total flavonoid content (0.095 mgQE/g sample).
Pengaruh Kombinasi Konsentrasi Pupuk Daun Bayfolan dan Ekstrak Kecambah Kacang Hijau/ Tauge (Vigna radiata L.) Terhadap Pertumbuhan Tanaman Buncis Tegak (Phaseolus vulgaris [L.] cv. Balitsa 2) Tia Setiawati; Maulidiyah Maulidiyah; Mohamad Nurzaman; Asep Zainal Mutaqin
EduMatSains : Jurnal Pendidikan, Matematika dan Sains Vol 2 No 2 (2018): Januari
Publisher : Fakultas Keguruan dan Ilmu Pendidikan, Universitas Kristen Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/edumatsains.v2i2.605

Abstract

The growth of bean plants can be enhached through the provision of fertilizer and organic material. The purpose of this research is to get the best concentration of Bayfolan leaf fertilizer and mung bean sprout (Vigna radiata L.)extract in improving the growth of green bean plants(Phaseolus vulgaris [L.] cv. Balitsa 2). This study was conducted using an experimental method in the greenhouse with a single factor Block Randomized design (BRD) consisting of 20 combinations of Bayfolan fertilizer and the extract of mung bean sprouts (V. radiata). Bayfolan fertilizer concentration used was 0, 2, 2.5, and 3 mL / L, while the concentration of bean sprout extract used was 0%, 20%, 40%, 60%, and 80%. The results of the Analysis of Variance (ANOVA) showed that the combination of 3 mL/L Bayfolan foliar fertilizer and 60% extract of mung bean sprouts was the best concentrations for the growth of green bean plants on plant height parameters, the number of leaves, the number of flowers, wide of leaf, and the weight of dried bush bean plants. Keywords: Phaseolus vulgaris, extract of mung bean sprouts, Bayfolan fertilizer, growth.